RESUMEN
As the most aggressive and malignant glioma, glioblastoma multiforme (GBM) abnormally expresses genes that mediate glycolytic metabolism and tumour cell growth. In this study, we investigated myricetin incorporated nanoliposomes and ascertained their prospect in effectively treating cancer via the employment of the GBM cell line DBTRG-05MG. Notably, the myricetin nanoliposomes (MYR-NLs) displayed potent inhibition of proliferation and significantly regulated the levels of proteins related to both glycolytic metabolism and cell survival. Most importantly, SIRT3 and phosphorylated p53 were also down-regulated by MYR-NLs, indicating that the MYR-NLs inhibited GBM cell growth through the SIRT3/p53-mediated PI3K/Akt-ERK and mitochondrial pathways. Our findings thus provide rational evidence that liposomal myricetin targeted at alternative cell death pathways may be a useful adjuvant therapy in glioblastoma treatment.
Asunto(s)
Apoptosis/efectos de los fármacos , Neoplasias Encefálicas , Flavonoides , Glioblastoma , Glucólisis/efectos de los fármacos , Nanopartículas , Proteínas de Neoplasias/biosíntesis , Sirtuina 3/biosíntesis , Neoplasias Encefálicas/tratamiento farmacológico , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patología , Línea Celular Tumoral , Flavonoides/química , Flavonoides/farmacología , Glioblastoma/tratamiento farmacológico , Glioblastoma/metabolismo , Glioblastoma/patología , Humanos , Liposomas , Nanopartículas/química , Nanopartículas/uso terapéuticoRESUMEN
With the development of medical radiography, cone beam computed tomography (CBCT) has been widely used in stomatology because of its numerous advantages, such as adequate spatial resolution, low radiation, undistorted three- dimensional tissue information, and good applicability for hard tissue imaging. Traditional periapical radiography is based on two-dimensional image which displays the three-dimensional object and its surrounding structures. Periapical radiography can only show the overlapping mesio-distally direction bone destruction, can't show buccolingual direction bone destruction. As a kind of three-dimensional imaging technology, CBCT can show three-dimensional structure of the organization from sagittal, coronal and axial direction, to overcome the defect of two-dimensional image such as overlap and deformation. CBCT has a unique advantage than periapical radiography in the diagnosis and treatment for periapical disease. This review will summarize the difference between CBCT and periapical radiography in the periapical disease.