Source Apportionment, Hydrodynamic Influence, and Environmental Stress of Pharmaceuticals in a Microtidal Estuary with Multiple Outlets in South China.

Pharmaceutical residues in the environment are of great concern as ubiquitous emerging contaminants. This study investigated the presence of 40 pharmaceuticals in water and sediment of the Pearl River Estuary (PRE) in the wet season of 2020. Among psychiatric drugs, only diazepam was found in water samples while six of them were detected in the sediment. The Σantibiotics levels ranged from 6.18 to 35.9 ng/L and 2.63 to 140 ng/g dry weight in water and sediment samples, respectively. Fluoroquinolones and tetracyclines were found well settling in the outlet sediment, while sulfonamides could be released from disturbed sediment under stronger tidal wash-out conditions. After entering the marine waters, pharmaceuticals tended to deposit at the PRE mouth by the influence of the plume bulge and onshore invasion of deep shelf waters. Low ecological risks to the aquatic organisms and of causing antimicrobial resistance were identified. Likewise, hydrological modeling results revealed insignificant risks: erythromycin-H2O and sulfamethoxazole discharged through the outlets constituted 30.8% and 6.74% of their environmental capacity, respectively. Source apportionment revealed that pharmaceutical discharges through the Humen and Yamen outlets were predominantly of animal origin. Overall, our findings provide strategic insights on environmental regulations to further minimize the environmental stress of pharmaceuticals in the PRE.

Occurrence and Characteristics of Microplastic Pollution in Xiangxi Bay of Three Gorges Reservoir, China.

Microplastic pollution in inland waters is receiving growing attentions. Reservoirs are suspected to be particularly vulnerable to microplastic pollution. However, very limited information is currently available on pollution characteristics of microplastics in reservoir ecosystems. This work studied the distribution and characteristics of microplastics in the backwater area of Xiangxi River, a typical tributary of the Three Gorges Reservoir. Microplastics were detected in both surface water and sediment with concentrations ranging from 0.55 × 105 to 342 × 105 items km-2 and 80 to 864 items m-2, respectively. Polyethylene, polypropylene, and polystyrene were identified in surface water, whereas polyethylene, polypropylene, and polyethylene terephthalate, and pigments were observed in sediment. In addition, microplastics were also detected in the digestion tracts of 25.7% of fish samples, and polyethylene and nylon were identified. Redundancy analysis indicates a weak correlation between microplastics and water quality variables but a negative correlation with water level of the reservoir and Secchi depth. Results from this study confirm the presence of high abundance microplastics in reservoir impacted tributaries, and suggest that water level regulated hydrodynamic condition and input of nonpoint sources are important regulators for microplastic accumulation and distribution in the backwater area of reservoir tributaries.

Microplastics in Hong Kong's marine waters: Impact of rainfall and Pearl River discharge.

This study provided a systematic investigation of microplastics in Hong Kong's surface marine waters during the pandemic from 2019 to 2021. Microplastics (2.07 ± 4.00 particles/m3) exhibited significant temporal variations with higher abundance in the wet season, without a consistent trend after the mandatory mask-wearing requirement was announced. The impact of pandemic restrictions on microplastic distribution was found to be relatively minor. However, significant correlations between microplastic abundances and rainfall highlighted the substantial contribution of local emissions through surface runoff. Notably, sites in closer proximity to the Pearl River Delta exhibited higher microplastic abundances, indicating their association with emission sources. The influence of rainfall and adverse weather on marine microplastic loads demonstrated different sensitivities among various locations but can generally last for one month. These results revealed the impact of seasonal rainfall on coastal microplastics and emphasized the need for efforts to reduce microplastic discharge from land-based sources.

Microplastic occurrence and ecological risk assessment in the eight outlets of the Pearl River Estuary, a new insight into the riverine microplastic input to the northern South China Sea.

Estuaries are unique transition zones connecting terrestrial and coastal environments and are recognized as primary conveyors for land-derived plastics to open oceans. Riverine microplastics (MPs) have been commonly investigated using sequential sampling which might not effectively reflect the actual load. In this study, sampling at eight outlets was performed during a complete tidal cycle to estimate the MP flux to the Pearl River Estuarine (PRE) using a concurrent sampling strategy. The MP abundances ranged from 2.90 ± 0.57-5.9 ± 2.27 particles/L. A remarkable difference between tides in MP abundances suggests tidal effect should not be overlooked in assessment. The MP load through the eight outlets was estimated at 304 trillion particles or 1102 tons into the PRE annually. Additionally, similar potential ecological risk assessment among eight rivers implied that environmental threats posed by less urbanized and populated rural areas on the western side have been under-evaluating for decades.

Microplastics: A major source of phthalate esters in aquatic environments.

Phthalate esters (PAEs) are predominant additives in plastics, their widespread contamination in aquatic environments has raised global concern. Here, twelve plastic products were prepared as microplastics to investigate their release behaviors of PAEs. Six out of 15 PAEs were quantified after 14 days of incubation in water. The leaching potentials were plastic type-specific, where the pencil case (polyvinyl chloride, PVC) represented the highest migrations with total ∑15 PAEs concentration of 6660 ± 513 ng/g, followed by the cleaning brush-1 (polyamide, PA, ~1830 ng/g) and rubber glove (1390 ± 57.5 ng/g). Conversely, the straw (polypropylene, PP), cleaning brush-2 (polyethylene terephthalate, PET) and shampoo bottle (PET) released the lowest amounts of PAEs, with 50.3 ± 8.21, 93.9 ± 91.8 and 104.35 ng/g, respectively. The release patterns of PAE congeners were polymer type-related, where di-n-butyl phthalate (DBP) dominated the leaching from PA, PP and PET microplastics (47-84%), diethyl phthalate leached the most from PVC and rubber microplastics (45-92%), while diisobutyl phthalate and DBP dominated the leaching from PE microplastics (68-94%). Water chemical properties could affect PAEs migration and the kinetic leaching process was well fitted with the pseudo-first-order model. Approximately 57.8-16,100 kg/year of PAEs were estimated to be released into oceans from microplastics.

Microplastic occurrence in the northern South China Sea, A case for Pre and Post cyclone analysis.

Microplastics (MPs) have become a great concern owing to their persistence and ecotoxicity in various environmental compartments. MPs can be transported from land to oceans via the aquatic system, and the oceans are believed to be the final sink for MPs. To resolve the lack of data concerning MP contamination in the northern South China Sea (NSCS), coastal seawater and sediments were investigated. The sample sites included the coastal area from Hong Kong to western Guangdong Province, which provided a representative coverage of variable distance to the shore. MP particles were observed in all samples, suggesting a widespread MP pollution in NSCS. The average MP abundance was relatively high in South China Sea, but it was lower than that in other Chinese coastal areas. In all samples, the dominant MP polymer was polyethylene terephthalate fibers. No statistically significant correlation was found between the abundances of MPs in seawater and sediment. The MP distribution in surface water affected by the monsoon exhibited high abundance in the east and low in the west, and MPs were more abundant near the shore than away from it. The tropical cyclone had no obvious effect on the overall MP abundance except in the Hong Kong west marine water (R4), which may attribute to proximity to Hong Kong and other islands.

Significant riverine inputs of typical plastic additives-phthalate esters from the Pearl River Delta to the northern South China Sea.

Phthalate esters (PAEs) are representative additives used extensively in plastics. In this study, 15 PAEs were investigated at the eight riverine outlets of the Pearl River Delta (PRD). The total concentrations of Σ15PAEs, including both the dissolved and particulate phases, ranged from 562 to 1460 ng/L and 679 ng/L-2830 ng/L in the surface and bottom layers, respectively. Dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP) dominated in the dissolved and suspended particulate matter (SPM) phases, respectively, accounting for >50 % and > 80 % of Σ15PAEs. Riverine input of wastewater from the PRD was possibly the primary source of the contamination. Higher levels of PAEs occurred at the eastern outlets than at the western ones. The dissolved and particulate PAEs varied seasonally, with significantly higher concentrations observed in the dry season than in the wet season. However, no significant differences of PAE levels in both phases were observed among low, medium, and high tides. The partitioning results demonstrated that SPM is important in the transportation of pollutants in estuaries, where more hydrophobic DEHP was predominantly transported by the SPM phase, while those more hydrophilic ones were regularly transported by the dissolved phase. The total annual flux of Σ15PAEs through the eight outlets to the SCS reached 1390 tons.

Occurrence, spatial distribution, and partitioning behavior of marine lipophilic phycotoxins in the Pearl River Estuary, South China.

The occurrence, spatial distribution, and partitioning behavior of 17 marine lipophilic phycotoxins (MLPs) in surface and bottom seawater, particulate organic matter (POM), and surface sediment from the Pearl River Estuary (PRE) were investigated to understand current contamination and the potential risks to marine ecosystems in this region. Nine MLPs were detected, including azaspiracid1-3, gymnodimine, okadaic acid, dinophysistoxin 1-2, pectenotoxin2 (PTX2), and homoyessotoxin, with Σ17MLP concentrations ranging 545-12,600 pg L-1 and 619-8,800 pg L-1 in surface and bottom seawater, respectively; 0-294 ng g-1 and 0.307-300 ng g-1 dry weight (dw) in surface and bottom POM, respectively; and 3.90-982 pg g-1 dw in surface sediment. Lower Σ17MLP levels in the seawater were found at the mouth of the PRE, and gradually increased with increasing distance offshore. According to the calculated partition coefficient, the affinity of MLPs for the aquatic environment components was as follows (from highest to lowest): POM > seawater > sediment. Overall, the distribution and migration of MLPs in the PRE may depend on partition coefficients, the organic carbon fraction, and environmental factors.

A systematic study of microplastic occurrence in urban water networks of a metropolis.

The release of microplastics from sewage treatment works (STWs) into the oceans around coastal cities is well documented. However, there are fewer studies on the microplastic abundance in stormwater drains and their emissions into the coastal marine environment via sewage and stormwater drainage networks. Here, we comprehensively investigated microplastic abundance in 66 sewage and 18 sludge samples collected from different process stages at three typical STWs and 36 water samples taken from six major stormwater drains during the dry and wet seasons in Hong Kong, which is a metropolitan city in south China. The results showed that microplastics were detected in all the sewage and stormwater samples, with the abundance ranging from 0.07 to 91.9 and from 0.4 to 36.48 particles/L, respectively, and in all the sludge samples with the abundance ranging from 167 to 936 particles/g (d. w.). There were no significant seasonal variations in the microplastic abundance across all samples of sewage, sludge, and stormwater. For both waterborne sample types, a smaller size (0.02-0.3 mm) and fiber shape were the dominant characteristics of the microplastics. Polyethylene terephthalate (PET) and polypropylene (PP) were the most abundant polymer types in the sewage samples, while polyethylene (PE), PET, PP, and PE-PP copolymer were the most abundant polymer types in the stormwater samples. The estimated range of total daily microplastic loads in the effluent from STWs in Hong Kong is estimated to be 4.48 × 109 - 2.68 × 1010 particles/day, demonstrating that STWs are major pathways of microplastics in coastal environments despite the high removal percentage of microplastics in sewage treatment processes examined. This is the first comprehensive study on microplastics in the urban waters of a coastal metropolis. However, further studies on other coastal cities will enable an accurate estimation of the microplastic contribution of stormwater drains to the world's oceans.

Understanding plastic degradation and microplastic formation in the environment: A review.

Plastic waste are introduced into the environment inevitably and their exposure in the environment causes deterioration in mechanical and physicochemical properties and leads to the formation of plastic fragments, which are considered as microplastics when their size is < 5 mm. In recent years, microplastic pollution has been reported in all kinds of environments worldwide and is considered a potential threat to the health of ecosystems and humans. However, knowledge on the environmental degradation of plastics and the formation of microplastics is still limited. In this review, potential hotspots for the accumulation of plastic waste were identified, major mechanisms and characterization methods of plastic degradation were summarized, and studies on the environmental degradation of plastics were evaluated. Future research works should further identify the key environmental parameters and properties of plastics affecting the degradation in order to predict the fate of plastics in different environments and facilitate the development of technologies for reducing plastic pollution. Formation and degradation of microplastics, including nanoplastics, should receive more research attention to assess their fate and ecological risks in the environment more comprehensively.

Intra-day microplastic variations in wastewater: A case study of a sewage treatment plant in Hong Kong.

Wastewater treatment plants (WWTPs) in coastal region play a primary role in transferring microplastics into the marine environment. Wastewater is closely related to anthropogenic activities, thus the intra-day variation of abundance of microplastics in the influent should be large and could have significant impact on their estimation of the daily mass load. In this study, a 2-hour interval sampling campaign was conducted at a secondary WWTP in Hong Kong to investigate the intra-day variations and daily loads of microplastics in influent. Results show that the average microplastic abundances increased from 7.1 ± 6.0 to 12.8 ± 5.8 particles/L over time, with predominant particle sizes ranging 1-5 mm. Approximately 80% of the microplastics in samples collected from 9:30-15:00 were polyethylene and polyester, while most samples collected at 17:00 were polypropylene and polyurethane. Microplastic loads exhibited large intra-day variations ranging 6.60 × 108-1.16 × 109 particles/day, indicating that calculated daily microplastic loads based on a specific sampling period may inaccurately estimate the actual daily load.

The hydro-fluctuation belt of the Three Gorges Reservoir: Source or sink of microplastics in the water?

Reservoirs can be an important environmental compartment for microplastic pollution. Previous investigations have found that surface waters and sediments in the Three Gorges Reservoir (TGR) have had high microplastic abundance, and the Xiangxi River, which is one of the largest primary tributaries of the TGR, has had much higher microplastic abundance than several marine and freshwater systems in China. A strip of land on the bank of the reservoir area, which is called the hydro-fluctuation belt (HFB), is periodically exposed due to the special hydrodynamic conditions in the TGR. The HFB may be an important source and/or sink of microplastics in TGR. In this study, microplastic occurrence in sediments from the Xiangxi River HFB was investigated to reflect the local microplastic pollution status and to evaluate its potential to serve as a source/sink of microplastics in the TGR. Seven sampling sites were selected, and sediments within the HFB and above the belt were collected in summer when the water level was low. The results showed that the microplastic abundance ranged from 0.55 ±â€¯0.12 × 103 to 14.58 ±â€¯5.67 × 103 particles m-2, which was one to two orders of magnitude higher than that in sediments from the Xiangxi River in our previous study (80-846 particles m-2). Statistical analysis revealed that the microplastic abundance within the HFB was significantly higher than that of the area above the HFB. The results indicate that the HFB can be an important microplastic sink when the water level is low, and the belt can turn into a potential source when the water level is high. Cluster analysis was applied to reveal the characteristics of the microplastics collected at different sites, and the results suggest that the cluster analysis may be a useful tool in elucidating the source and fate of microplastics.

Microplastics in the intestinal tracts of East Asian finless porpoises (Neophocaena asiaeorientalis sunameri) from Yellow Sea and Bohai Sea of China.

The direct evidences for the ingestion of microplastics by cetaceans, especially the cetaceans in Asian marine areas are limited. In this study, residue of microplastics in the intestinal tracts of East Asian finless porpoises (Neophocaena asiaeorientalis sunameri) was investigated. Microplastics were detected in all specimens, with mean abundance of 19.1 ±â€¯7.2 items/individual. With respect to microplastics properties, fibers, blue items, and polypropylene were predominant in shapes, colors, and plastic materials, respectively. Trophic transfer and unintentional ingestion might be the potential pathways for microplastics ingested by finless porpoise. The specific intestinal structure might account for the predominance of fibers and the accumulation of microplastics at the beginning portion of intestines. This study indicates that cetaceans in Chinese marine areas also suffer from microplastics pollution. Further studies on the fate and ecological effects of microplastics should be conducted to reveal their potential risks to cetaceans.

Uptake and depuration of paralytic shellfish toxins in the green-lipped mussel, Perna viridis: a dynamic model.

Uptake and depuration of paralytic shellfish toxins in the green-lipped mussel, Perna viridis, were investigated by exposing the mussels to dinoflagellates (Alexandrium tamarense, ACTI01) under laboratory conditions for 8 d, then depurating them in clean seawater for 14 d. First-order linear differential equations were set up for five tissue compartments: Viscera, gill, hepatopancreas, adductor muscle, and foot. The solutions to these equations were used to fit the experimental data. We then estimated the parameters governing the model, which depend on the elimination rate from each compartment and the transfer coefficient between compartments. An assumption of the model is that the gills transport the dinoflagellates directly to the mouth and then to the viscera, where the ingested cells are broken down, releasing the toxins. The toxins absorbed are transferred to other tissues. During the uptake phase, the transfer coefficients from viscera to gill, hepatopancreas, adductor muscle, and foot were 0.03, 0.24, 0.01, and 0.004 per day, respectively. During the depuration phase, the transfer coefficients were 0.01, 0, 0.01, and 0.003 per day, respectively. In terms of the anatomical distribution of N-sulfocarbamoyl-11-hydroxysulfate (C2) toxins in various tissues, viscera and hepatopancreas contained the highest percentages (47-74% and 8-41%, respectively). Together, these two tissue compartments accounted for 71 to 96% of all C2 toxins present. The biokinetic model allows a quantitative prediction of C2 toxins in whole ussel as well as individual tissue compartments based on the density estimates and toxin load of dinoflagellate cells in the surrounding waters over time.

Uptake and depuration of PAHs and chlorinated pesticides by semi-permeable membrane devices (SPMDs) and green-lipped mussels (Perna viridis).

Semi-batch seawater experiments were conducted to follow the uptake and release of selected PAHs (anthracene, fluorathene, pyrene and B[a]P) and organochlorine pesticides (alpha-HCH, aldrin, dieldrin, p,p'-DDT) in semi-permeable membrane devices (SPMDs) and green-lipped mussels (Perna viridis). Mathematical models were applied to describe the uptake and elimination curves of the contaminants for SPMDs, and kinetic parameters, such as uptake rate constants, and equilibrium triolein/water partitioning coefficients were calculated. SPMD data showed a good fit to estimate rate constant and partition coefficient equations, but only those contaminants which partitioned mainly in the dissolved phase (alpha-HCH and dieldrin) were well explained for mussels. Poor conformity of the other contaminants indicated mussels uptake by routes other than diffusion, such as ingestion of algae. An apparent equilibrium state was only noted for alpha-HCH in mussels. Aldrin was not detected in mussels in the first few days of exposure, indicating potential metabolism of this compound. B[a]P was not detected in the triolein of SPMDs, which suggests that the membrane may act as a reservoir. Loss of spiked B[a]P from the triolein was evident in a depuration experiment, which may indicate transfer to the membrane. Rate constants for mussels were higher than those for SPMDs, but the reverse was true for partition coefficients. Overall, mussels and SPMDs had similar uptake rates for all compounds in this study, excluding p,p'-DDT and dieldrin. Contaminant elimination took place more rapidly in mussels, implying that SPMDs are better candidates for detecting episodic discharge of organic contaminants.

A comparison of polycyclic aromatic hydrocarbon and petroleum hydrocarbon uptake by mussels (Perna viridis) and semi-permeable membrane devices (SPMDs) in Hong Kong coastal waters.

The ability of mussels (Perna viridis) and semi-permeable membrane devices (SPMDs) to accumulate polycyclic aromatic hydrocarbons (PAHs) and petroleum hydrocarbons (PHCs) from five sites in Hong Kong's coastal waters was compared. Mussels consistently had higher levels of contaminants, but their utility was limited at one highly polluted site due to mortality. Mussels and SPMDs ranked sites differently in terms of individual contaminant levels. Although SPMDs overcome many of the disadvantages of using living organisms to measure contaminants in marine waters, they cannot be used as "mimics" due to different PAH and PHC accumulation patterns.

Distribution and behavior of trace metals in the sediment and porewater of a tropical coastal wetland.

Vertical profiles (0-30 cm below surface) of four trace metals-Cadmium (Cd), Chromium (Cr), Lead (Pb) and Zinc (Zn)-in the sediment and sediment porewater of an ecologically important intertidal mudflat in the Mai Po and Inner Deep Bay Ramsar Site were thoroughly studied over a period of 10 months (from March 1999 to January 2000). Two surveys, one in summer and another in winter, involving a total of eight sampling stations were conducted to study the seasonal variation of the remobilization characteristics of these trace metals in the mudflat sediment. The range of depth averaged concentration of these trace metals in the mudflat sediment was: 0.3-0.8 microg/g (Cd); 9.8-91.0 microg/g (Cr); 7.3-69.1 microg/g (Pb); and 39.5-192.0 microg/g (Zn), while that in the sediment porewater was: 0.3-121.1 microg/l (Cd); 3.0-2704.1 microg/l (Cr); 2.6-105.6 microg/l (Pb); and 32.6-4238.3 microg/l (Zn). In general, levels of dissolved trace metals in the sediment porewater were much higher in the summer than in the winter while their concentrations in the sediment were more or less the same throughout the year. Enrichment of Cd, Pb and Zn in the sediment porewater of the upper oxic layer and that of Cr in the oxic-sub-oxic boundary was generally observed. Regions in the vicinity of the Mai Po mangroves and the river mouths of Shenzhen River and Shan Pui River were found to be hotspots of trace metal pollution. Benthic diffusive fluxes of trace metals from the mudflat sediment were also estimated. Of the four trace metals, cadmium showed the greatest tendency toward remobilization from the sediment phase to the more bio-available porewater phase.

Biofouling confounds the uptake of trace organic contaminants by semi-permeable membrane devices (SPMDs).

The outer layers of layflat, low density polyethylene plastic tubing (the principal component of semi-permeable membrane devices, SPMDs) were biofouled at a clean site in Hong Kong coastal waters for periods of 1-4 weeks. Following pre-fouling, triolein was added to the SPMDs and, along with control (unfouled) devices, they were exposed to a range of organochlorine pesticides (alpha-HCH, aldrin, p,p'-DDT) and PAHs (anthracene, fluoranthene and benzo(a)pyrene) under laboratory conditions. Results showed that the uptake of contaminants by SPMDs was severely reduced by as much as 50% under fouling conditions in comparison to unfouled controls. The ultimate utility of SPMDs as passive monitors is thus reduced, although alternative measures, such as the use of permeability reference compounds may compensate, and allow for realistic evaluations of dissolved environmental concentrations in aquatic environments. However, due to the complexities involved in such procedures--especially as they need to be conducted on a case-by-case basis-the utility of SPMDs appears to be limited for estimates of bioavailability unless necessary calibrations are undertaken within each environment that the sampler is used.