Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome.

Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrophilic nanofibers with an aligned topography by incorporating polyethylene glycol and polycaprolactone using axial electrospinning. We investigated the effect of the nanofibers on macrophage behavior and the underlying mechanisms. With the increase of hydrophilicity of aligned nanofibers, the inflammatory gene expression of macrophages adhering to them was downregulated, and M2 polarization was induced. We further presented clear evidence that the inflammasome NOD-like receptor thermal protein domain associated protein 3 (NLRP3) was the cellular sensor by which macrophages sense the biomaterials, and it acted as a regulator of the macrophage-mediated response to foreign bodies and implant integration. In vivo, we showed that the fibers shaped the implant-related immune microenvironment and ameliorated peritendinous adhesions. In conclusion, our study demonstrated that hydrophilic aligned nanofibers exhibited better biocompatibility and immunological properties.

Elaborate design of shell component for manipulating the sustained release behavior from core-shell nanofibres.

BACKGROUND: The diversified combination of nanostructure and material has received considerable attention from researchers to exploit advanced functional materials. In drug delivery systems, the hydrophilicity and sustained-release drug properties are in opposition. Thus, difficulties remain in the simultaneous improve sustained-release drug properties and increase the hydrophilicity of materials. METHODS: In this work, we proposed a modified triaxial electrospinning strategy to fabricate functional core-shell fibres, which could elaborate design of shell component for manipulating the sustained-release drug. Cellulose acetate (CA) was designed as the main polymeric matrix, whereas polyethylene glycol (PEG) was added as a hydrophilic material in the middle layer. Cur, as a model drug, was stored in the inner layer. RESULTS: Scanning electron microscopy (SEM) results and transmission electron microscopy (TEM) demonstrated that the cylindrical F2-F4 fibres had a clear core-shell structure. The model drug Cur in fibres was verified in an amorphous form during the X-ray diffraction (XRD) patterns, and Fourier transformed infrared spectroscopy (FTIR) results indicated good compatibility with the CA matrix. The water contact angle test showed that functional F2-F4 fibres had a high hydrophilic property in 120 s and the control sample F1 needed over 0.5 h to obtain hydrophilic property. In the initial stage of moisture intrusion into fibres, the quickly dissolved PEG component guided the water molecules and rapidly eroded the internal structure of functional fibres. The good hydrophilicity of F2-F4 fibres brought relatively excellent swelling rate around 4600%. Blank outer layer of functional F2 fibres with 1% PEG created an exciting opportunity for providing a 96 h sustained-release drug profile, while F3 and F4 fibres with over 3% PEG provided a 12 h modified drug release profile to eliminate tailing-off effect. CONCLUSION: Here, the functional F2-F4 fibres had been successfully produced by using the advanced modified triaxial electrospinning nanotechnology with different polymer matrices. The simple strategy in this work has remarkable potential to manipulate hydrophilicity and sustained release of drug carriers, meantime it can also enrich the preparation approaches of functional nanomaterials.

Electrochemical reduction of Cr (VI) using a palladium/graphene modified stainless steel electrode.

In this study, a palladium/graphene modified stainless steel electrode was successfully prepared and applied in an electrochemical reduction device to remove Cr (VI) from the wastewater. Pd was modified onto the electrode mainly via interacting with the carboxyl group of graphene. The Cr (VI) removal efficiency was up to 99.70 ± 0.00% under the optimal condition (Pd content proportion of 3%, electrode potential of -0.9 V, pH = 2 and electrolyte concentration of 6 g/L). It was found that Cr (VI) was removed via the following processes: (1) direct electrochemical reduction by accepting electrons, (2) indirect electrochemical reduction by H2O2 that was generated from H2 in the presence of Pd, (3) adsorption through hydrogen bond, and (4) chemical reduction through alkoxy groups donating electrons. The indirect electrochemical reduction considerably promoted the Cr (VI) removal while a small amount of Cr (VI) was removed via adsorption and chemical reduction. The method could not only be used as a pretreatment technology to solve the problem of excessive Cr (VI) concentration of industrial wastewater, but also could provide reference for the electrochemical reduction of similar metal ions.

Electrospun PVP-Core/PHBV-Shell Fibers to Eliminate Tailing Off for an Improved Sustained Release of Curcumin.

Tailing off release in the sustained release of water-insoluble curcumin (Cur) is a significant challenge in the drug delivery system. As a novel solution, core-shell nanodrug containers have aroused many interests due to their potential improvement in drug-sustained release. In this work, a biodegradable polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and hydrophilic polyvinylpyrrolidone (PVP) were exploited as drug delivery carriers by coaxial electrospinning, and the core-shell drug-loaded fibers exhibited improved sustained release of Cur. A cylindrical morphology and a clear core-shell structure were observed by scanning and transmission electron microscopies. The X-ray diffraction pattern and infrared spectroscopy revealed that Cur existed in amorphous form due to its good compatibility with PHBV and PVP. The in vitro drug release curves confirmed that the core-shell container manipulated Cur in a faster drug release process than that in the traditional PHBV monolithic container. The combination of the material and structure forms a novel nanodrug container with a better sustained release of water-insoluble Cur. This strategy is beneficial for exploiting more functional biomedical materials to improve the drug release behavior.

Study on the Formation and Properties of Trapped Nanobubbles and Surface Nanobubbles by Spontaneous and Temperature Difference Methods.

Trapped nanobubbles are gas domains trapped at nanopits on the solid-liquid interface. This is different from surface nanobubbles that usually form at the smooth surface. Herein, both trapped nanobubbles and surface nanobubbles formed on the nanopitted polystyrene film were studied by a spontaneous formation method and a temperature difference method. Trapped nanobubbles behave more flexibly than surface nanobubbles under different scanning loads. The nanopits under trapped nanobubbles appear after being subjected to large force scanning, and both trapped nanobubbles and surface nanobubbles can recover after reducing the scan load. The contact angles of the two kinds of nanobubbles were calculated and were found to be approximately constant. Configurations of trapped nanobubbles including under the pit mouth, protruding out but pinning at the pit mouth, and protruding out and extending around the pit mouth were experimentally observed. Gas oversaturation in the liquid after replacing the low-temperature water with high-temperature water was evaluated and was found to be a key factor for nanobubble formation and led to trapped nanobubbles protruding out and extending. Our study should be helpful in understanding the formation mechanism and properties of trapped nanobubbles and surface nanobubbles, and it will also be useful for further research on the control of nanobubble distribution.

Electrospun multi-chamber core-shell nanofibers and their controlled release behaviors: A review.

Core-shell structure is a concentric circle structure found in nature. The rapid development of electrospinning technology provides more approaches for the production of core-shell nanofibers. The nanoscale effects and expansive specific surface area of core-shell nanofibers can facilitate the dissolution of drugs. By employing ingenious structural designs and judicious polymer selection, specialized nanofiber drug delivery systems can be prepared to achieve controlled drug release. The synergistic combination of core-shell structure and materials exhibits a strong strategy for enhancing the drug utilization efficiency and customizing the release profile of drugs. Consequently, multi-chamber core-shell nanofibers hold great promise for highly efficient disease treatment. However, little attention concentration is focused on the effect of multi-chamber core-shell nanofibers on controlled release of drugs. In this review, we introduced different fabrication techniques for multi-chamber core-shell nanostructures, including advanced electrospinning technologies and surface functionalization. Subsequently, we reviewed the different controlled drug release behaviors of multi-chamber core-shell nanofibers and their potential needs for disease treatment. The comprehensive elucidation of controlled release behaviors based on electrospun multi-chamber core-shell nanostructures could inspire the exploration of novel controlled delivery systems. Furthermore, once these fibers with customizable drug release profiles move toward industrial mass production, they will potentially promote the development of pharmacy and the treatment of various diseases. This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies.

N-doped carbon fibers in situ prepared by hydrothermal carbonization of Camellia sinensis branches waste for efficient removal of heavy metal ions.

N-doped carbon fibers (NCFs) were in situ prepared by Camellia sinensis branches waste through hydrothermal carbonization with urea/ZnCl2 at 160-280 °C under 0.8-8.9 MPa. The structural characteristics of NCFs were investigated by elemental analysis, SEM, TEM, XRD, XPS, Raman spectra, and BET surface area. The highest N content of NCFs obtained at 280 °C was 8.96%, and the main forms of doped N were pyridinic N, pyrrolic N, and graphitic N. Moreover, NCFs were applied to remove metal ions successfully. The results showed that NCF-240 had the maximum adsorption amounts of 106.52, 125.23, and 153.49 mg/g for Cu2+, Pb2+, and Zn2+, respectively, while NCF-280 had the best removal ability on Cr6+ (145.67 mg/g). Finally, it demonstrated that the adsorption behavior of NCFs was well fitted by the pseudo-second-order kinetic and the Langmuir adsorption isotherm models.

Characterizing disease manifestations and treatment outcomes among patients with orofacial granulomatosis in China.

BACKGROUND: Racial variation exists in the incidence of orofacial granulomatosis (OFG). The epidemiology and clinical characteristics of OFG in Asian countries are poorly described. OBJECTIVE: To describe the epidemiologic and clinical features of OFG in China from data collected on chronic odontogenic infection and studied in actual practice regarding the long-term outcome of OFG patients receiving different treatments. METHODS: Data on demographics, medical history, chronic odontogenic infection, and the extent of disease were collected, and long-term outcomes after the end of treatments were evaluated. RESULTS: Of the 165 OFG patients, 118 (71.5%; 95% CI 64.6%-78.5%) had a chronic odontogenic infection. There was a variety of difference between OFG with and without chronic odontogenic infection. Approximately 98.3% (95% confidence interval 94.8%-100%) of OFG patients with chronic odontogenic infection who received dental treatment showed a marked response, of whom 31 patients (53.4%; 95% confidence interval 40.2%-66.7%) had complete remission. LIMITATIONS: Endoscopic investigations were not performed for most of the patients, and more detailed data were not collected, which might have demonstrated additional systemic problems. CONCLUSIONS: OFG with chronic odontogenic infection is the major clinical pattern of OFG in China, which may be a subtype of OFG. Dental treatment should necessarily be the preferred first-line therapy for such patients.

Induction of autophagy protects human dental pulp cells from lipopolysaccharide-induced pyroptotic cell death.

The NOD-like receptor protein 3/caspase-1 inflammasome can be activated in human dental pulp tissue and fibroblasts; however, the underlying mechanisms are poorly understood. In the present study, lipopolysaccharide (LPS) was used to treat dental pulp cells to establish an inflammation model. Cell viability was examined by sulforhodamine B assay. Interleukin (IL)-1ß, caspase-1, microtubule-associated protein-1 light chain 3-II/I and p62 were determined by western blotting and ELISA. The phosphorylation (p-) levels of NF-κB and NF-κB inhibitor (IκB)α protein were observed by western blotting. The results demonstrated that LPS induced pyroptotic cell death in cultured dental pulp cells, which was supported by the increased levels of IL-1ß, IL-18 and caspase-1. Rapamycin and 3-methyladenine (3-MA) were used to activate and inhibit autophagy, and it was observed that LPS increased autophagy and rapamycin reduced LPS-induced dental pulp cell pyroptosis. However, 3-MA aggravated LPS-induced dental pulp cell pyroptosis. In addition, LPS inhibited the expression of IκBα, but increased the expression of p-NF-κB. Compared with the LPS group, 3-MA further inhibited the expression of IκBα but promoted the expression of p-NF-κB. However, rapamycin produced the opposite results to LPS. Under LPS treatment, the NF-κB pathway inhibitor BAY11-7082 further enhanced the inhibitory effects of rapamycin, but inhibited the promoting effects of 3-MA on the protein expression levels of IL-1ß and caspase-1. The results of the present study demonstrated that there is an important crosstalk between autophagy, pyroptosis and the NF-κB pathway, and that the modulation of pyroptosis in dental pulp cells may be a promising strategy to pulpitis therapy.

A closed concept of extractive whole cell microbial transformation of benzaldehyde into L-phenylacetylcarbinol by Saccharomyces cerevisiae in novel polyethylene-glycol-induced cloud-point system.

Extractive microbial transformation of benzaldehyde into L-phenylacetylcarbinol (L-PAC) by Saccharomyces cerevisiae (Baker's yeast) has been carried out in a novel polyethylene-glycol-induced cloud-point system (PEG-CPS). The extractive microbial transformation in the PEG-CPS and a downstream process for stripping of the product from the microbial transformation broth with microemulsion extraction are demonstrated. The results indicate that the PEG-CPS maintains the advantage of CPS for in situ extraction of polar product in the microbial transformation. At the same time, the utilization of hydrophilic nonionic surfactant in the PEG-CPS is favorable for stripping of product from the nonionic surfactant in the microbial transformation broth by Winsor I microemulsion extraction. Thus, a closed concept of in situ extraction of polar product in microbial transformation and its downstream process of product recovery are fulfilled at the same time.