Efficacy and safety of a novel submucosal injection solution for endoscopic resection in porcine models.

OBJECTIVE: A submucosal injection is usually required to improve the efficacy and safety of endoscopic submucosal dissection (ESD) and endoscopic mucosal resection (EMR). This study aimed to evaluate the performance of 3.3% sodium carboxymethyl starch (Na-CMS) solution, a novel submucosal injection solution, for ESD and EMR. METHODS: Na-CMS, normal saline (NS) and two commercially available agents (sigMAVisc and Eleview) were injected into the esophageal submucosa of randomly grouped pigs. The level of submucosal elevation was examined. Subsequently, ESD or EMR procedures using 3.3% Na-CMS or NS as submucosal injections were performed in the gastrointestinal tract of the pigs. RESULTS: Submucosal elevation was significantly higher and more sustained in the 3.3% Na-CMS group than in the controls (P < 0.05). The volume required for ESD or EMR was significantly lower in the 3.3% Na-CMS group than in the NS group (ESD: 12.21 ± 4.09 mL vs 28.25 ± 8.02 mL, P < 0.001; EMR: 3.99 ± 1.98 mL vs 7.15 ± 3.67 mL, P = 0.001). The ESD resection time was significantly shorter in the 3.3% Na-CMS group than in the NS group (16.58 ± 7.30 min vs 25.29 ± 11.89 min, P = 0.004). Hemorrhage after ESD in the 3.3% Na-CMS group was less severe than that in the NS group (P = 0.006). CONCLUSION: 3.3% Na-CMS is an effective, safe and low-cost submucosal injection solution and holds promise as preferable agent for submucosal injection in ESD and EMR procedures.

[Effects of optimal nitrogen fertilizer management on water and fertilizer utilization efficiency and yield under double-ridge-furrow sowing with the whole plastic film mulching in maize in a semi-arid area]. / åŠå¹²æ—±åŒºæ°®è‚¥è¿ç­¹å¯¹å ¨è†œåŒåž„æ²Ÿæ’­çŽ‰ç±³æ°´è‚¥åˆ©ç”¨å’Œäº§é‡çš„å½±å“.

Improper fertilization style is one of the main reasons for low water and fertilizer use efficiency of double-ridge-furrow sowing with the whole plastic film mulching in maize production in the semi-arid area. Understanding the effects of reduction, postponing, and organic fertilizer substitution of nitrogen fertilizer on water and fertilizer use efficiency and yield of maize can provide theore-tical basis for effective management of water and fertilizer in maize production. Based on a 4-year field experiment with three treatments: all fertilizers as base fertilizer under double-ridge-furrow sowing with the whole plastic film mulching (CK), nitrogen fertilizer reduced by 15% and topdres-sing in tasseling stage (RN), 30% of the chemical fertilizer replaced by organic fertilizer and topdressing in tasseling stage (RNM), we measured water consumption characteristics, growth and development, water and fertilizer utilization efficiency of maize. The results showed that fertilization pattern significantly affected water and fertilizer utilization efficiency and yield of maize, which was dependent on annual rainfall. In dry and normal rainfall year, water consumption in pre-flowering stage of RN was decreased by 16.1%-18.8% and that in post-flowering stage was increased by 18.0%-22.2%, while water consumption in pre-flowering and post-flowering stages of RNM did not differ from that in CK. In wet year, water consumption in pre-flowering stage of RN and RNM was decreased by 16.7% and 6.3%, while that in post-flowering stage was increased by 11.4% and 29.7%, respectively. Compared with CK, RN significantly increased the relative content of chlorophyll (SPAD) of maize leaves after topdressing, the biomass in post-flowering stage was increased by 15.6%-44.9%, the ear length, the number and weight of grains per spike and the 100-grain weight were increased significantly, yield was increased by 9.8%-17.0%, and water use efficiency (WUE) was increased by 6.3%-21.4%, with the partial productivities of fertilizer (PEPT), N (PEPTN), P (PEPTP) and K (PEPTK) were all increased significantly. In conclusion, RN could improve water consumption and the SPAD value in post-flowering stage of maize in different precipitation years, increase post-flowering biomass, and optimize the ear character, obviously improve yield, water and fertilizer use efficiency. It was a effective fertilizer management mode with high-efficiency utilization of water and fertilizer under double-ridge-furrow sowing with the whole plastic film mulching in maize in the semi-arid area.

[Effects of micro-ridge-furrow with plastic mulching and bunching seeding on soil hydrothermal environment and its response to photosynthesis and grain yield of spring wheat]. / å ¨è†œå¾®åž„æ²Ÿç©´æ’­å¯¹æ˜¥å°éº¦åœŸå£¤æ°´çƒ­çŽ¯å¢ƒçš„å½±å“åŠå ¶å ‰åˆå’Œäº§é‡æ•ˆåº”.

The relieving of drought and cold restriction on spring wheat development is one of the key factors increasing wheat yield in arid areas of central Gansu Province. A field experiment with spring wheat (Longchun No. 35) was carried out in central Gansu Province from 2016 to 2018. There were three treatments: 1) micro-ridge-furrow with whole field plastic mulching and bunching seeding (PRF), 2) whole field soil plastic mulching and bunching seeding (PMS), 3) bunching seeding without mulching (CK). We measured soil temperature in 0-25 cm profile, soil water content in 0-300 cm profile, leaf SPAD, photosynthetic rate, transpiration rate, aboveground biomass in different growth stages, and grain yield to understand the effect of PRF on soil hydrothermal environment, spring wheat yield and water use efficiency (WUE) from the aspect of soil hydrothermal, canopy development and grain yield. The results showed that mean soil temperature in 0-25 cm profile of PRF and PMS increased by 2.8 ℃ and 2.5 ℃ at the seedling stage, decreased by 1.4 ℃ and 0.9 ℃ from filling to maturity stage, respectively. Soil water storage in 0-300 cm profile of PRF and PMS increased by 59.7 mm and 41.8 mm from sowing to seedling stage. Water consumption of PRF and PMS increased by 46.1 mm and 39.8 mm from seedling to filling stage. PRF increased average soil temperature in 0-25 cm profile by 0.3 ℃ at seedling stage, but decreased by 0.5 ℃ from filling to maturity stage, and increased soil water storage in 0-300 cm profile by 18.0 mm from sowing to seedling stage. PMF increased water consumption by 13.0 mm from booting to maturing stage, as compared with PMS. Based on the optimizated soil hydrothermal conditions, leaf SPAD value, aboveground biomass, net photosynthetic rate, and transpiration rate of PRF increased, as compared with PMS and CK. The PRF increased grain yield by 9.1% and 36.5%, WUE by 5.9% and 30.8% compared to PMS and CK, respectively. Consequently, PRF increased soil temperature at wheat seedling stage, reduced it from filling to maturing stage, improved wheat water consumption from sowing to filling stage, increased leaf SPAD value and aboveground biomass, promoted photosynthetic function in leaf from seedling to filling stage, and consequently led to increased yield and water utilization. Such effects were more significant in dry year (2016 and 2017).

[Effects of organic fertilizer application on flag leaf C/N ratio, photosynthetic characteristics and yield of spring wheat with full plastic film mulching]. / å ¨è†œè¦†åœŸä¸‹æ–½æœ‰æœºè‚¥å¯¹æ˜¥å°éº¦æ——å¶ç¢³æ°®æ¯”ã€å ‰åˆç‰¹æ€§å’Œäº§é‡çš„å½±å“.

A field experiment was conducted in the rain-fed semi-arid region of central Gansu in 2016 and 2017, with the treatments 1) hill-drop flat planting with full plastic film mulching (PMS), 2) hill-drop flat planting with full plastic film mulching plus organic fertilizers (PMO), and 3) hill-drop flat planting without soil mulching (CK). We investigated the relations among soil moisture, photosynthetic rate (Pn), stomatal conductance (gs) and transpiration rate (Tr), C/N ratio, and total nitrogen of flag leaf from the heading stage to the seed-filling stage in different treatments to probe into their effects on the yield and yield components of spring wheat variety 'Longchun 27'. The results showed that organic fertilizer application could increase soil moisture at the middle and late growth stages of spring wheat. PMO increased soil water storage in 0-300 cm depth from the heading stage to the seed filling stage by 4.6% and 8.5%, decreased population canopy temperature by 0.1-1.3 ℃ and 1.4-4.9 ℃, increased net photosynthetic rate of flag leaf by 9.3% and 29.7%, stomatal conductance by 30.9% and 103.8%, transpiration rate by 5.1% and 55.0%, total nitrogen content by 6.6% and 18.9%, and decreased C/N ratio by 6.4% and 22.8%, respectively. Compared with PMS and CK, PMO significantly improved grain number per spike and 1000-grain weight, and increased grain yield by 9.1% and 53.7%, respectively. From the heading stage to filling stage, the Pn and gs of flag leaf had negative correlation with C/N, while C/N was negatively correlated with grain yield. Consequently, PMO could improve soil water storage and promote photosynthesis of flag leaf, reduce the intensity of physiological drought stress and the limitations of nitrogen absorption and assimilation in flag leaf from the heading stage to the seed-filling stage, and increase grain number and grain weight and consequently the yield of spring wheat in semi-arid region.

[Effects of fertilizer application on water consumption characteristics and yield of potato cultured under ridge-furrow and whole filed plastic mulching in rain-fed area.] / æ–½è‚¥å¯¹æ—±åœ°å ¨è†œè¦†ç›–åž„æ²Ÿç§æ¤é©¬é“ƒè–¯è€—æ°´ç‰¹å¾åŠäº§é‡çš„å½±å“.

Chemical fertilizer reduction and organic manure substitution are the useful methods to increase potato water-and nutrient use efficiency, which is cultured under ridge-furrow and whole soil surface mulched by plastic film in semiarid rain-fed area. A 4-year field experiment was carried out from 2011 to 2014 with three treatments: 1) traditional chemical fertilizer application (F), 2) chemical nitrogen fertilizer reduced by 25% and dressing at flowering stage (DF), and 3) chemical nitrogen fertilizer reduced by 50% and organic manure substitution (OF). The soil moisture and potato yield were investigated, and seasonal water consumption, water use efficiency (WUE) were calculated to study the regulations of different nutrient management methods on potato water use process, as well as its effects on potato tuber yield and WUE. The results showed that soil water storage in potato flowering stage was the highest under DF treatment, but there were no significant difference among these three treatments. The depth of soil water depletion in DF and OF showed an increasing trend at post-flowering stage. Potato water consumption decreased significantly at pre-flowering stage, but increased by 36.2%, 23.2%, 24.8% and 19.0% respectively at post-flowering stage in 2011-2014 under DF treatment, as compared with those under F treatment. OF treatment increased potato water consumption by 20.7% and 16.3% than that under F treatment at post-flowering stage from 2011 to 2012, respectively, but exerted no significant effect at pre-flowering stage. Compared with F, DF increased potato tuber yield averagely by 2595.1 kg·hm-2 from 2012 to 2014 and significantly increased the WUE by 14.4% and 6.3% in 2013 and 2014, respectively; OF significantly increased potato tuber yield averagely by 2945 kg·hm-2 tuber yield in 4 experimental years and WUE was significantly higher than that under F from 2012 to 2014. It was indicated that both DF and OF could regulate water consumption between pre-flowering and post-flowering stages, and increase potato tuber yield and WUE. OF showed more significant effects than DF on the increment of tuber yield and WUE.

Delivery of DNA-based cancer vaccine with polyethylenimine.

DNA-based vaccine directed to tumor-specific antigens is an attractive strategy in cancer prevention and therapy. In view of the poor immunogenicity of most tumor-associated antigens, studies were carried out here to examine the adjuvant effect of polyethylenimine (PEI), a cationic polymer widely used in non-viral gene delivery, on the efficacy of cancer vaccine strategy. Treatment of animals with the DNA/PEI complexes resulted in antigen-specific cell lysis and activation of B3Z cells, an ovalbumin/K(b)-specific cytotoxic clone that recognizes the target cells through the class I major histocompatibility complex (MHC) molecules. Immunohistochemical examination showed that PEI-mediated DNA vaccination induced cell death and significant lymphocyte infiltration at the injection sites. Immunization of C57BL/6J mice with the DNA/PEI complexes either preceded or after tumor cell inoculation suppressed tumor growth and prolonged the survival rate of the animals. Results obtained in this study illustrated the potential use of PEI as an adjuvant in DNA-based cancer vaccination for induction of protective and therapeutic immunity.