Effects of polyethylene, polyvinyl chloride, and polystyrene microplastics on the vermitoxicity of fluoranthene in soil.

Polycyclic aromatic hydrocarbons (PAHs) and microplastics (MPs) may coexist in soil. Consequently, the toxicity of PAHs to "soil engineers" earthworms (vermitoxicity), may be influenced by various MPs. However, studies on this issue are scarce. In particular, the differential effect of MPs of different polymers on the vermitoxicity of PAHs remains unclear. Therefore, a series of microcosm experiments were conducted to evaluate the potential vermitoxicity of fluoranthene (Fla) in the presence of polyethylene (PE), polyvinyl chloride (PVC), and polystyrene (PS) MPs at an environmentally relevant concentration (125 mg kg-1). The results indicated that Fla exerted vermitoxicity and MPs enhanced the adverse effects. Specifically, after the introduction of MPs, the 14-d LC50s of Fla on earthworms decreased from 130.9 to 98.0-123.6 mg kg-1; in addition, the growth inhibition rates improved from 5.37%-15.34% to 15.63%-33.38%, and the avoidance rate increased by 10%-100% at the same exposure doses in most cases. In most cases, the neutral red retention time was shortened by 14.3%-47.9%, indicating that the integrity of the coelomocytes' lysosomal membrane had worsened. The affected antioxidant enzyme activity and improved malondialdehyde content indicated enhanced oxidative damage in the treatments containing MPs. However, the aggravation of the vermitoxicity varied by MPs type and toxicological endpoints. Overall, Fla + PS MPs exerted the greatest effect on the mortality of earthworms. On the contrary, PVC and PE MPs exhibited higher subacute effects on the vermitoxicity of Fla. Our study also demonstrated that MPs at environmentally relevant doses may directly induce vermitoxicity. In particular, damaged coelomocytes' lysosomal membrane stability by MPs was second reported to the best of our knowledge. Our results revealed the differences in the effects of various MPs on the vermitoxicity of PAHs, which provides new data in assessing the ecological effects of PAHs and MPs in soils.

A safe and novel method for video-assisted thoracic surgery preoperative localization of small pulmonary nodules by using ZT medical glue (2-octyl cyanoacrylate).

BACKGROUND: Accurate and fast localization of small pulmonary nodules is required for local pulmonary resection. In this study, we introduced and assessed a novel technique for the preoperative localization of small pulmonary nodules by using ZT medical glue (2-octyl cyanoacrylate). METHODS: 101 patients who had a combined total of 106 small pulmonary nodules located by ZT glue and 53patients with 53 small pulmonary nodules located by hookwire were selected. Guided by computed tomography (CT), the surgeon injected certain volume ZT glue into an area adjacent to the small pulmonary nodule, then, the adjacent lung tissue infiltrated by ZT glue formed into a depressed hard nodule which can be used for preoperative localization with an obvious mark on lung surface or different hand touch. After localization, Wedge resection was performed via video-assisted thoracoscopic surgery and the specimen obtained from the procedure was immediately sent for pathological examination, followed by a standard surgical procedure. A contrast has been made between the ZT glue method and the hookwire. RESULTS: 101 operations were successfully performed by using this novel technique, and 106 small pulmonary nodules were successfully located. Compared with the hookwire location, ZT glue method obviouslyextended the Time interval between localization and operation (P = 0.00) and a same complication rate (P = 0.07). CONCLUSIONS: The use of ZT glue is a safe and effective method for the localization of small pulmonary nodules. TRIAL REGISTRATION: This study was approved by the ethics committee of Shaoxing People's Hospital (Number:2016-004, Date:2016,2,24), and informed consent was obtained from all enrolled patients.