Characterization and 3D printing of a biodegradable polylactic acid/thermoplastic polyurethane blend with laccase-modified lignin as a nucleating agent.

Polylactic acid (PLA) has been used in fused deposition method (FDM) based 3D printing for many years. Alkali lignin is an undervalued industrial by-product that could upgrade PLA's poor mechanical properties. This work presents a biotechnological approach consisting of a partial degradation of alkali lignin using Bacillus ligniniphilus laccase (Lacc) L1 for its use as a nucleating agent in a polylactic acid/thermoplastic polyurethane (PLA/TPU) blend. Results showed that adding enzymatically modified lignin (EL) increased the elasticity modulus to a maximum of 2.5-fold than the control and conferred a maximum biodegradability rate of 15 % after 6 months under the soil burial method. Furthermore, the printing quality rendered satisfactory smooth surfaces, geometries and a tunable addition of a woody color. These findings open a new door for using laccase as a tool to upgrade lignin's properties and its use as a scaffold in manufacturing more environmentally sustainable filaments with improved mechanical properties for 3D printing.

Lignin valorization: Status, challenges and opportunities.

As an abundant aromatic biopolymer, lignin has the potential to produce various chemicals, biofuels of interest through biorefinery activities and is expected to benefit the future circular economy. However, lignin valorization is hindered by a series of constraints such as heterogeneous polymeric nature, intrinsic recalcitrance, strong smell, dark colour, challenges in lignocelluloses fractionation and the presence of high bond dissociation enthalpies in its functional groups etc. Nowadays, industrial lignin is mostly combusted for electricity production and the recycling of inorganic compounds involved in the pulping process. Given the research and development on lignin valorization in recent years, important applications such as lignin-based hydrogels, surfactants, three-dimensional printing materials, electrodes and production of fine chemicals have been systematically reviewed. Finally, this review highlights the main constraints affecting industrial lignin valorization, possible solutions and future perspectives, in the light of its abundance and its potential applications reported in the scientific literature.