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1.
J Prosthodont ; 33(3): 273-280, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36973859

RESUMEN

PURPOSE: This in vitro study evaluated the fracture strength of screw-retained zirconia crowns connected to zirconia (Zr) and titanium (Ti) implants after undergoing a simulation of 5 years of clinical use. MATERIALS AND METHODS: Forty-eight screw-retained zirconia crowns were fabricated and assembled on four implant systems, with 12 in each group: (1) Zr implant (pure ceramic; Straumann AG) (PZr); (2) Zr implant (NobelPearl; Nobel Biocare) (NPZr); (3) Ti-Zr implant (Bone Level Roxolid; Straumann AG) (RSTiZr); (4) Ti implant (Conical Connection PMC; Nobel Biocare) (NRTi). Crowns were luted to their associated abutments using resin cement and then torqued to their assigned implants at the recommended torque value. Specimens were subjected to dynamic loading for 1,200,000 loading cycles. Fracture strength, measured in Newtons (N), was tested under static compression load using a universal testing machine at an angle of 30°. One-way ANOVA and Tukey's multiple comparisons post hoc test were used to compare the mean fracture values between the groups at a significance level of 0.05. RESULTS: The average fracture strengths for the RSTiZr and NRTi groups were 1207 ± 202 and 1073 ± 217 N, respectively, which was significantly (p < 0.0001) higher than the PZr and NPZr groups (712 ± 76 and 571.6 ± 167 N, respectively). However, no significant difference was found between the fracture strength value of RSTiZr and NRTi (p = 0.260) or PZr and NPZr (p = 0.256) groups. CONCLUSIONS: Zirconia crowns connected to Zr implants have the potential to withstand the average physiological occlusal forces which occur in the anterior and premolar regions.


Asunto(s)
Implantes Dentales , Porcelana Dental , Resistencia Flexional , Titanio , Ensayo de Materiales , Pilares Dentales , Fracaso de la Restauración Dental , Análisis del Estrés Dental , Coronas , Circonio
2.
Clin Oral Implants Res ; 34(10): 1106-1117, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37485737

RESUMEN

OBJECTIVES: To assess accuracy of intraoral optical scanning (IOS) and stereophotogrammetry (SPG), complete-arch digital implant impressions in vivo. MATERIALS AND METHODS: Consecutive patients needing implant-supported screw-retained zirconia complete-arch fixed-dental prostheses (ISZ-FDP) were recruited. For each patient, three impressions were taken: IOS, SPG (tests), and open-tray plaster (reference). Linear (ΔX, ΔY, and ΔZ), three-dimensional (ΔEUC), and angular deviations (ΔANGLE) were evaluated and stratified according to scanning technology for each implant. Potential effects of impression device (IOS and SPG), arch (maxilla and mandible), and implant number (4 and 6) were evaluated through multivariable analysis. Significance level was set at .05. RESULTS: A total of 11 complete arches (5 maxillae, 6 mandibles) in 11 patients were rehabilitated with ISZ-FDPs supported by 4 (n = 8) and 6 implants (n = 3). A total of 50 implants and 100 implant positions were captured by two investigated devices and compared to respective reference (mean ΔEUC IOS 137.2, SPG 87.6 µm; mean ΔANGLE 0.79, 0.38°). Differences between measurements (SPG-IOS) were computed for each implant, with negative values indicating better SPG accuracy. Significant mean ΔEUC difference of -49.60 µm (p = .0143; SD 138.15) and mean ΔANGLE difference of -0.40° (p < .0001; SD 0.65) were observed in favor of SPG. Multivariable analysis showed significant effect on ΔEUC (p = .0162) and ΔANGLE (p = .0001) only for impression devices, with SPG performing better. CONCLUSIONS: SPG experienced significantly higher linear and angular accuracy. No effect of type of arch or implant number was detected. Higher extreme deviations were experienced for IOS. SPG can be feasible for complete-arch digital impressions with caution, and rigid prototype try-in is recommended before screw-retained prosthesis manufacturing.

3.
Clin Oral Investig ; 27(9): 5041-5048, 2023 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-37421492

RESUMEN

OBJECTIVES: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects. MATERIALS AND METHODS: A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated. RESULTS: The test group yielded statistically significantly (p = 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (p = 0.94). CONCLUSIONS: The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing. CLINICAL RELEVANCE: The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.


Asunto(s)
Recesión Gingival , Humanos , Animales , Porcinos , Amelogenina/farmacología , Porcinos Enanos , Recesión Gingival/tratamiento farmacológico , Recesión Gingival/cirugía , Cicatrización de Heridas , Cemento Dental , Resultado del Tratamiento , Raíz del Diente/patología , Tejido Conectivo
4.
J Prosthet Dent ; 130(2): 187-201, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34750013

RESUMEN

STATEMENT OF PROBLEM: As socket grafting with commercially available biomaterials has become popular, reports of the root submergence technique for ridge preservation have decreased. A systematic review of this partial extraction therapy is lacking. PURPOSE: The purpose of this systematic review was to review the root submergence technique as well as critically appraise the available data. MATERIAL AND METHODS: A review was carried out that observed the Participant, Intervention, Comparison, Outcomes (PICO) strategy and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The focused question was "What are the outcomes of the different methods to submerge tooth roots for ridge preservation?" Medical subject headings (MeSH) terms that related to the root submergence technique were searched in PubMed/MEDLINE, Scopus, and the Cochrane Library databases. RESULTS: A total of 7709 abstracts and study titles were individually screened from the initial search results. After reviewing the full-text articles and applying the selection criteria, the final included search results totaled 47 full-text articles for in-depth review. In 10 animal studies, 258 roots were studied in 34 dogs and 7 monkeys. Histological data confirmed that coronal bridging (bone or cementum growth over the cut root) was a common outcome. Of the vital roots submerged, the majority maintained their vitality. In 37 human studies, 475 roots were submerged and reported on. Subjective ridge preservation was often reported. Among the adverse healing outcomes, exposure of the root through the mucosa was the most common. Nonetheless, in animals, 86.8% of roots remained submerged; in humans, 74.7%. CONCLUSIONS: Root submergence is an established technique for ridge preservation. Exposure is a common complication, and correct technique may be key to its prevention. Further research of this partial extraction therapy is encouraged.


Asunto(s)
Aumento de la Cresta Alveolar , Alveolo Dental , Animales , Perros , Humanos , Proceso Alveolar/cirugía , Aumento de la Cresta Alveolar/métodos , Materiales Biocompatibles , Extracción Dental/métodos , Alveolo Dental/cirugía
5.
J Prosthet Dent ; 130(2): 146-154, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34895903

RESUMEN

Submerging roots for ridge preservation was the earliest partial extraction therapy and has been described since the 1970s. Despite the approximately 47 animal and human studies published since, an updated and contemporary step-by-step protocol has not yet been provided. This technique report describes in detail how to manage submerged roots at single tooth sites and at short-span edentulous areas.


Asunto(s)
Extracción Dental , Alveolo Dental , Animales , Humanos , Extracción Dental/métodos , Raíz del Diente/diagnóstico por imagen , Raíz del Diente/cirugía
6.
BMC Oral Health ; 20(1): 351, 2020 12 01.
Artículo en Inglés | MEDLINE | ID: mdl-33261592

RESUMEN

BACKGROUND: In the regenerative treatment of intrabony periodontal defects, surgical strategies are primarily determined by defect morphologies. In certain cases, however, direct clinical measurements and intraoral radiographs do not provide sufficient information on defect morphologies. Therefore, the application of cone-beam computed tomography (CBCT) has been proposed in specific cases. 3D virtual models reconstructed with automatic thresholding algorithms have already been used for diagnostic purposes. The aim of this study was to utilize 3D virtual models, generated with a semi-automatic segmentation method, for the treatment planning of minimally invasive periodontal surgeries and to evaluate the accuracy of the virtual models, by comparing digital measurements to direct intrasurgical measurements. METHODS: Four patients with a total of six intrabony periodontal defects were enrolled in the present study. Two months following initial periodontal treatment, a CBCT scan was taken. The novel semi-automatic segmentation method was performed in an open-source medical image processing software (3D Slicer) to acquire virtual 3D models of alveolar and dental structures. Intrasurgical and digital measurements were taken, and results were compared to validate the accuracy of the digital models. Defect characteristics were determined prior to surgery with conventional diagnostic methods and 3D virtual models. Diagnostic assessments were compared to the actual defect morphology during surgery. RESULTS: Differences between intrasurgical and digital measurements in depth and width of intrabony components of periodontal defects averaged 0.31 ± 0.21 mm and 0.41 ± 0.44 mm, respectively. In five out of six cases, defect characteristics could not be assessed precisely with direct clinical measurements and intraoral radiographs. 3D models generated with the presented semi-automatic segmentation method depicted the defect characteristics correctly in all six cases. CONCLUSION: It can be concluded that 3D virtual models acquired with the described semi-automatic segmentation method provide accurate information on intrabony periodontal defect morphologies, thus influencing the treatment strategy. Within the limitations of this study, models were found to be accurate; however, further investigation with a standardized validation process on a large number of participants has to be conducted.


Asunto(s)
Pérdida de Hueso Alveolar , Enfermedades Periodontales , Pérdida de Hueso Alveolar/diagnóstico por imagen , Tomografía Computarizada de Haz Cónico , Humanos , Procesamiento de Imagen Asistido por Computador , Imagenología Tridimensional , Enfermedades Periodontales/diagnóstico por imagen , Enfermedades Periodontales/cirugía , Radiografía
7.
J Prosthet Dent ; 121(4): 581-585, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30554825

RESUMEN

Partial extraction therapies, such as the socket-shield technique, use the patient's tooth tissues and periodontium to preserve the alveolar ridge and limit postextraction resorption. Internal exposure through the overlying peri-implant mucosa has been reported as the most common complication, suggesting that the preparation technique requires modification. This technique report describes the prosthetic management of the socket-shield technique, emphasizing preparation of the socket-shield to the bone crest, and the creation of an S-shape prosthetic emergence profile to support maximal soft tissue infill.


Asunto(s)
Pérdida de Hueso Alveolar , Implantes Dentales de Diente Único , Proceso Alveolar , Implantación Dental Endoósea , Humanos , Periodoncio , Prótesis e Implantes , Extracción Dental , Alveolo Dental
8.
J Prosthet Dent ; 121(2): 229-233, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30722985

RESUMEN

The socket-shield technique for avoiding postextraction tissue alteration was first described in 2010. The technique was developed for hopeless teeth in anterior esthetic sites but has not yet been described for molar sites. Managing postextractive ridge changes in the posterior region by prevention or regeneration remains a challenge. The socket shield aims to offset these ridge changes wherever possible, preserving the patient's residual tissues at immediate implants. This technique report describes the molar socket-shield step by step.


Asunto(s)
Carga Inmediata del Implante Dental , Estética Dental , Humanos , Diente Molar , Extracción Dental , Alveolo Dental
9.
Orv Hetil ; 159(37): 1516-1524, 2018 Sep.
Artículo en Húngaro | MEDLINE | ID: mdl-30196718

RESUMEN

INTRODUCTION: In a clinicopathological retrospective epidemiological study we investigated benign tumors and tumor-like lesions located in the orofacial region, diagnosed at the Universiy of Szeged, Department of Oral Medicine. METHOD: During a 54-year period (1960-2014), 14 661 biopsies were taken. The included subjects were 7491 patients diagnosed with benign tumors and tumor-like lesions. RESULTS: The average age of patients was 55.3 years, 2823 (37.7%) patients were male and 4668 (62.3%) female. The male : female ratio was 1 : 1.65. Most of the patients included in the study were aged 51-60 (1280, 17.1%). The number of children was 1014 (13.6%) and the number of adults was 6477 (86.3%). The number of non-neoplasms was 6420 (85.7%), being significantly higher than the number of neoplasms (1071, 14.3%). Most of the lesions were of mesenchymal origin (5574, 77.4%); the number of lesions of non-mesenchymal origin was 982 (13.1%). The most prevalent type of lesions was traumatic fibroma (fibrosis): 1806 (32.4%). The most common lesion type in the group of lesions of infectious/inflammational origin was pyogenic granuloma, the number of which was 465 (8.3%). The most common cystic lesion was mucocele (805, 10.7%). Hemangioma was the most frequent lesion type among developmental anomalies with the number of 815 (14.6%). The most common location of the lesions was the lip in 2081 cases (27.8%), followed by the gingiva in 2024 cases (27.0%), bucca in 1069 cases (14.3%), tongue in 981 cases (13.1%), and the facial skin in 695 cases (9.3%). After taking biopsy, the majority of benign lesions were treated with cryo-, laser-, or combined (cryo and laser) surgery. CONCLUSION: The present computer-aided study showed that irritational fibroma was the most common orofacial benign tumor, and the lip was the most frequent location. The diagnostic classification and the methodology are considerably different in the majority of the studies, which may hinder the exact comparison with other surveys from different regions of the world. Orv Hetil. 2018; 159(37): 1516-1524.


Asunto(s)
Enfermedades Maxilomandibulares/epidemiología , Enfermedades de la Boca/epidemiología , Medicina Oral , Adulto , Anciano , Biopsia/estadística & datos numéricos , Femenino , Fibroma/epidemiología , Humanos , Hungría , Masculino , Persona de Mediana Edad , Neoplasias de la Boca/epidemiología , Enfermedades Periapicales/epidemiología , Prevalencia , Estudios Retrospectivos , Enfermedades de las Glándulas Salivales/epidemiología , Factores Sexuales
10.
Orv Hetil ; 159(11): 415-422, 2018 Mar.
Artículo en Húngaro | MEDLINE | ID: mdl-29526126

RESUMEN

The aim of the present review is to provide an up-to-date picture of what we know about the connection between odontogenic foci and non-oral diseases. After a brief historical summary, we give an overview on how the odontogenic focus causes disease in distant areas of the body in general, and then we start the discussion of the particular conditions, such as cardiovascular diseases, pneumonia, diabetes mellitus, metabolic syndrome, rheumatoid arthritis and adverse pregnancy outcomes. The review is centered around the two main odontogenic foci: periodontitis and periapical periodontitis, the latter being a widely recognized but rarely discussed oral focus. Finally, we offer a few considerations that the practicing dentist may find useful when dealing with odontogenic foci. Orv Hetil. 2018; 159(11): 415-422.


Asunto(s)
Infecciones/etiología , Enfermedades Periodontales/complicaciones , Artritis Reumatoide/etiología , Enfermedades Cardiovasculares/etiología , Diabetes Mellitus/etiología , Susceptibilidad a Enfermedades , Femenino , Humanos , Masculino , Síndrome Metabólico/etiología , Neumonía Bacteriana/etiología , Embarazo , Complicaciones Infecciosas del Embarazo/etiología , Factores de Riesgo
11.
Orv Hetil ; 159(42): 1700-1709, 2018 10.
Artículo en Húngaro | MEDLINE | ID: mdl-30334485

RESUMEN

Dental amalgam has been used for more than 150 years due to its beneficial mechanical properties and durability in dentistry. In the past and to date, many questions about amalgam restorations have arisen, especially regarding the mercury content, which has been the subject of global disputes. By presenting the past and present of the 'amalgam issue', the aim of our paper is to display the current position of international literature. This summary is based on the publications in the PubMed database, the guidelines of the Council of European Dentists. Although the use of dental amalgam is widespread, concerns have been raised about the adverse effect on human health and the environment, focusing on its heavy metal pollution during waste treatment. In 2017, the European Union (EU) adopted the so-called Mercury Regulation, based on the United Nations Minamata Convention on Mercury, the recommendations of which are presented in the present review. This Regulation includes the requirement for EU Member States to develop a national action plan for the phase-down of amalgam. The feasibility plan for complete phase-out may be guaranteed by 2030. The authors discuss the advantages and disadvantages of possible amalgam alternatives by presenting glass-ionomers and resin-based composites. In the future, more material research programmes and long-term follow-up studies are necessary. In addition to several global health organizations, the Council of European Dentists also draws attention to prevent dental caries, expecting to reduce the number of restorations. Orv Hetil. 2018; 159(42): 1700-1709.


Asunto(s)
Resinas Acrílicas/uso terapéutico , Resinas Compuestas/uso terapéutico , Amalgama Dental/uso terapéutico , Restauración Dental Permanente/métodos , Resinas Acrílicas/efectos adversos , Resinas Compuestas/efectos adversos , Amalgama Dental/efectos adversos , Dentición Permanente , Humanos
12.
Intervirology ; 59(2): 123-129, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27924796

RESUMEN

OBJECTIVES: Because torque teno virus (TTV) has been implicated in tumorigenesis as a cocarcinogen, we studied TTV prevalence in saliva and biopsy samples from head and neck cancer (HNCC) patients, patients with premalignant lesions of oral cancer, and controls. We also wished to determine the TTV genotypes in HNCC patients. METHODS: A seminested polymerase chain reaction (PCR) amplifying the N22 region of the TTV genome, as well as direct sequencing of PCR fragments, was used. RESULTS: TTV prevalence was higher in HNCC patients (saliva: 27/71, 38%; tumor biopsy: 22/74, 30%) than in controls (saliva: 8/56, 14%; oral mucosa: 1/19, 5%). TTV prevalence was also high in patients with premalignant lesions of oral carcinoma (saliva: 9/18, 50%; biopsy: 5/21, 24%). By phylogenetic analysis, TTV belonging mostly to genotypes 1 and 2 was found in HNCC patients. In most of the cases, identical TTV strains were present in the biopsy and salivary sample of the same HNCC patient. In addition, the same TTV strain was detected in 2 laryngeal carcinoma biopsies obtained from 2 independent patients. CONCLUSIONS: Our data are compatible with the idea that TTV might act as a cocarcinogen in certain cases of HNCC. Alternatively, HNCC may facilitate either TTV replication or TTV entry into the saliva.


Asunto(s)
Infecciones por Virus ADN/epidemiología , Neoplasias de Cabeza y Cuello/virología , Saliva/virología , Torque teno virus/genética , Torque teno virus/aislamiento & purificación , Adulto , Biopsia , Infecciones por Virus ADN/diagnóstico , ADN Viral , Femenino , Genoma Viral , Genotipo , Humanos , Neoplasias Laríngeas/virología , Masculino , Persona de Mediana Edad , Neoplasias de la Boca/virología , Filogenia , Reacción en Cadena de la Polimerasa , Prevalencia , Glándulas Salivales/patología , Glándulas Salivales/virología , Torque teno virus/clasificación , Torque teno virus/fisiología
13.
Implant Dent ; 24(6): 675-9, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26134690

RESUMEN

OBJECTIVES: Acidic pH and high fluoride (F(-)) concentration impair the corrosion resistance of titanium (Ti). Caries-preventive products contain high amounts of F(-) and are applied at low pH. The purpose of this study was to evaluate whether fluoride applied in different forms has different short-, mid-, and long-term effects on the growth of the bacteria Streptococcus mutans. MATERIALS AND METHODS: Ti discs with polished surface were treated with a rinse containing 0.025% olaflur, a gel containing 1.25% olaflur, or a 1% aqueous solution of NaF (pH 4.5), and they were incubated with S mutans for 21 days. Control discs did not get prophylactic treatment. Protein assay analysis was performed at regular intervals to estimate the amount of S mutans. Scanning electron microscopic (SEM) images were also taken. RESULTS: Bacterial protein quantity became significantly different only by the 21st day. Fluoride in rinse and gel proved to be superior to NaF in aqueous solution or no treatment (P < 0.01 and P < 0.05, respectively). However, the discs treated with fluoride in gel showed signs of corrosion in SEM images. CONCLUSION: The results suggest that the use of fluoride-containing mouthwashes might be the best and safest oral hygienic choice for patients with oral implants. Furthermore, olaflur seems to be superior to NaF for long-term use at low pH.


Asunto(s)
Profilaxis Antibiótica/métodos , Fluoruros/uso terapéutico , Streptococcus mutans/efectos de los fármacos , Titanio , Diaminas/administración & dosificación , Diaminas/uso terapéutico , Fluoruros/administración & dosificación , Microscopía Electrónica de Rastreo , Fluoruro de Sodio/administración & dosificación , Fluoruro de Sodio/uso terapéutico , Streptococcus mutans/crecimiento & desarrollo , Factores de Tiempo
14.
Fogorv Sz ; 108(4): 115-9, 2015 Dec.
Artículo en Húngaro | MEDLINE | ID: mdl-26863815

RESUMEN

OBJECTIVES: The purpose of this in vitro investigation was to evaluate the reinforcing effect of different fibre-reinforced composite (FRC) posts and insertion techniques in premolar teeth when using minimal invasive post space preparation. MATERIALS AND METHODS: Thirty two extracted and endodontically treated premolar teeth were used and divided into four groups (n = 8) depending on the post used (Group 1-4). 1: one single conventional post, 2: one main conventional and one collateral post, 3: one flexible post, 4: one main flexible and one collateral post. After cementation and core build-up the specimens were submitted to static fracture toughness test. Fracture thresholds and fracture patterns were recorded and evaluated. RESULTS: The multi-post techniques (group 2 and 4) showed statistically higher fracture resistance compared to group one. Regarding fracture patterns there was no statistically significant difference between the tested groups. CONCLUSION: The application of multiple posts seems to be beneficial regarding fracture resistance independent from the used FRC post. Fracture pattern was not influenced by the elasticity of the post.


Asunto(s)
Diente Premolar , Implantes Dentales , Materiales Dentales , Vidrio , Ensayo de Materiales , Fracturas de los Dientes/prevención & control , Elasticidad , Humanos , Técnicas In Vitro , Proyectos Piloto , Tratamiento del Conducto Radicular , Extracción Dental
15.
Med Sci Monit ; 20: 476-80, 2014 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-24658020

RESUMEN

Alagille syndrome is a rare, autosomal, complex, dominant disorder associated with dysfunction of the liver, heart, skeleton, and eyes, as well as characteristic facial appearance. It is associated with the defect in component of the Notch signalling pathway. Here, we review the main features of Alagille syndrome with special focus on oro-facial manifestations like prominent forehead, moderate hypertelorism with deep-set eyes, a saddle or straight nose with a flattened, bulbous tip, and large ears. The article is based on the most recent and significant literature available from the Medline database. Contrary to healthy children, patients with Alagille syndrome have many problems, depending on several factors like the severity of cholestasis and scarring in the liver, heart or lung problems, presence of infections, or other problems related to poor nutrition that can manifest in their oral cavity in the dental and periodontal tissues, as well as oral mucosa. From the dentist's view, the most important elements are careful observation, accurate diagnosis, and planned management of such patients, especially during the patient's formative years, to prevent complications. Aggressive preventive oral care and consultations with medical specialists before any invasive procedure are obligatory. All this can improve quality of life in patients with Alagille syndrome.


Asunto(s)
Síndrome de Alagille/terapia , Salud Bucal , Dentición , Humanos , Especificidad de Órganos
16.
Clin Oral Investig ; 18(3): 829-38, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23907470

RESUMEN

AIMS: The aims of this double-blind, controlled, crossover study were to assess the influence of food preservatives on in situ dental biofilm growth and vitality, and to evaluate their influence on the ability of dental biofilm to demineralize underlying enamel over a period of 14 days. MATERIALS AND METHODS: Twenty volunteers wore appliances with six specimens each of bovine enamel to build up intra-oral biofilms. During four test cycles of 14 days, the subjects had to place the appliance in one of the assigned controls or active solutions twice a day for a minute: negative control 0.9 % saline, 0.1 % benzoate (BA), 0.1 % sorbate (SA) and 0.2 % chlorhexidine (CHX positive control). After 14 days, the biofilms on two of the slabs were stained to visualize vital and dead bacteria to assess biofilm thickness (BT) and bacterial vitality (BV). Further, slabs were taken to determine mineral loss (ML), by quantitative light-induced laser fluorescence (QLF) and transversal microradiography (TMR), moreover the lesion depths (LD). RESULTS: Nineteen subjects completed all test cycles. Use of SA, BA and CHX resulted in a significantly reduced BV compared to NaCl (p < 0.001). Only CHX exerted a statistically significant retardation in BT as compared to saline. Differences between SA and BA were not significant (p > 0.05) for both parameters. TMR analysis revealed the highest LD values in the NaCl group (43.6 ± 44.2 µm) and the lowest with CHX (11.7 ± 39.4 µm), while SA (22.9 ± 45.2 µm) and BA (21.4 ± 38.5 µm) lay in between. Similarly for ML, the highest mean values of 128.1 ± 207.3 vol% µm were assessed for NaCl, the lowest for CHX (-16.8 ± 284.2 vol% µm), while SA and BA led to values of 83.2 ± 150.9 and 98.4 ± 191.2 vol% µm, respectively. With QLF for both controls, NaCl (-33.8 ± 101.3 mm(2) %) and CHX (-16.9 ± 69.9 mm(2) %), negative values were recorded reflecting a diminution of fluorescence, while positive values were found with SA (33.9 ± 158.2 mm(2) %) and BA (24.8 ± 118.0 mm(2) %) depicting a fluorescence gain. These differences were non-significant (p > 0.05). CONCLUSION: The biofilm model permited the assessment of undisturbed oral biofilm formation influenced by antibacterial components under clinical conditions for a period of 14 days. An effect of BA and SA on the demineralization of enamel could be demonstrated by TMR and QLF, but these new findings have to be seen as a trend. As part of our daily diet, these preservatives exert an impact on the metabolism of the dental biofilm, and therefore may even influence demineralization processes of the underlying dental enamel in situ.


Asunto(s)
Biopelículas , Esmalte Dental , Conservantes de Alimentos , Desmineralización Dental , Animales , Bovinos
17.
Acta Odontol Scand ; 72(3): 216-27, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23964635

RESUMEN

OBJECTIVES: The role was studied of multiple single nucleotide polymorphisms in tooth agenesis in the Hungarian population using a complex approach. METHODS: Eight SNPs, PAX9 -912 C/T, PAX9 -1031 A/G, MSX1 3755 A/G, FGFR1 T/C rs881301, IRF6 T/C rs764093, AXIN2-8150 A/G, AXIN2-8434 A/G and AXIN2-30224 C/T, were studied in 192 hypodontia and 17 oligodontia cases and in 260 healthy volunteers. Case-control analysis was performed to test both allelic and genotypic associations as well as associations at the level of haplotypes. Multivariate exploratory Bayesian network-based multi-level analysis of relevance (BN-BMLA) as well as logistic regression analysis were performed. RESULTS: Conventional statistics showed that PAX9 SNP -912 C/T and the MSX1 SNP changed the incidence of hypodontia, although after Bonferroni correction for multiple hypothesis testing, the effects were only borderline tendencies. Using a statistical analysis better suited for handling multiple hypotheses, the BN-BMLA, PAX9 SNPs clearly showed a synergistic effect. This was confirmed by other multivariate analyses and it remained significant after corrections for multiple hypothesis testing (p < 0.0025). The PAX9-1031-A-PAX9-912-T haplotype was the most relevant combination causing hypodontia. Interaction was weaker between PAX9 and MSX1, while other SNPs had no joint effect on hypodontia. CONCLUSION: This complex analysis shows the important role of PAX9 and MSX1 SNPs and of their interactions in tooth agenesis, while IRF6, FGFR1 and AXIN2 SNPs had no detectable role in the Hungarian population. These results also reveal that risk factors in hypodontia need to be identified in various populations, since there is considerable variability among them.


Asunto(s)
Polimorfismo de Nucleótido Simple , Enfermedades Dentales/genética , Teorema de Bayes , Genética de Población , Humanos , Hungría
18.
Fogorv Sz ; 107(4): 115-23, 2014 Dec.
Artículo en Húngaro | MEDLINE | ID: mdl-25730939

RESUMEN

The aim of the study was to evaluate the knowledge of dental trauma management in the southern part of Hungary among parents, teachers, medical doctors and dentists. A cross-sectional study was performed among the mentioned groups, with a structured questionnaire. The participants consisted of 275 people, most of them were parents (185), the other groups were smaller (30-30). 73.62% of parents recognised that the maxillary incisor of a 9-year-old girl is probably a permanent tooth. In case of the coronal fracture of this tooth 65.03% responded correctly. The correct response rate for the management of an avulsed tooth was only 39.26%, but in case of loss of consciousness 92.64% would act correctly. The Chi-square test indicated that there was a significant association between the correct recognition of the type of incisor and higher education (p < 0.01), and the number of seen TDIs. The 76.7% of the parents was not satisfied with his knowledge about TDLs, and 74.9% was interested in more information. In our study the parents performed generally better compared to other studies, but compared to the other three groups their results were worse. The knowledge of the teachers and the doctors was almost equivalent. The dentist had the best results. The level of knowledge of dental trauma management was inadequate among parents, but the teachers and doctors should improve their knowledge too. We suggest that it's necessary to start educational campaigns among groups that could be the primary caregivers of traumatic dental injuries.


Asunto(s)
Odontólogos/estadística & datos numéricos , Docentes/estadística & datos numéricos , Conocimientos, Actitudes y Práctica en Salud , Padres , Traumatismos de los Dientes/diagnóstico , Traumatismos de los Dientes/terapia , Adulto , Distribución de Chi-Cuadrado , Escolaridad , Femenino , Educación en Salud Dental , Humanos , Hungría/epidemiología , Incisivo/lesiones , Masculino , Persona de Mediana Edad , Encuestas y Cuestionarios , Avulsión de Diente/diagnóstico , Avulsión de Diente/terapia , Fracturas de los Dientes/diagnóstico , Fracturas de los Dientes/terapia , Inconsciencia/diagnóstico , Inconsciencia/terapia
19.
Fogorv Sz ; 107(3): 87-92, 2014 Sep.
Artículo en Húngaro | MEDLINE | ID: mdl-25509509

RESUMEN

Papillon-Lefèvre syndrome, a rare disease with autosomal recessive inheritance, is characterized by aggressive periodontitis and palmoplantar hyperkeratosis. Mutations of the cathepsin C gene are responsible for the development of the disease. In this study, we aimed to describe in details the clinical symptoms and to determine the underlying genetic abnormality in two Hungarian siblings affected by Papillon-Lefèvre syndrome. The siblings are under regular dental and dermatological care since their symptoms appeared, but, due to the fact that genetic analysis of Papillon-Lefèvre syndrome has been available for one or two years in Hungary, their mutation screenings were just recently performed. We have identified a homozygous missense mutation on the cathepsin C gene, which is an already published mutation and was originally reported from Germany. Our investigations would like to draw attention to a rare disease, Papillon-Lefèvre syndrome, in which first symptom can be the aggressive periodontitis, and in which genetic testing and for helping child-bearing and family planning is now available.


Asunto(s)
Catepsina C/genética , Boca Edéntula/genética , Boca Edéntula/rehabilitación , Mutación Missense , Enfermedad de Papillon-Lefevre/genética , Adulto , Periodontitis Agresiva/genética , Periodontitis Agresiva/rehabilitación , Europa (Continente) , Femenino , Humanos , Hungría , Enfermedad de Papillon-Lefevre/diagnóstico , Linaje , Análisis de Secuencia de ADN
20.
J Prosthodont Res ; 68(1): 172-180, 2024 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-37574278

RESUMEN

PURPOSE: To assess and compare the accuracies of intraoral scanners (IOS) and stereophotogrammetry (SPG) devices for complete-arch digital implant impressions. METHODS: A 4-analog model was digitized using a desk scanner to obtain a reference file. Thirty test scans were conducted using the investigated IOS device, while an additional 30 scans were performed using the SPG device. Using the best-fit algorithm, the resulting 60 test files were aligned with the reference file. Linear (ΔX, ΔY, and ΔZ-axis) and angular deviations (ΔANGLE) were evaluated. Three-dimensional (3D) deviation was calculated based on the Euclidean distance (ΔEUC). The analysis was stratified according to the scanning device and implant position. Fisher's F and t-tests were used to compare the variances and expected values of the two scanning systems. RESULTS: IOS expressed a higher 3D (ΔEUC) mean deviation than SPG (52.8 µm vs. 33.4 µm, P < 0.0001), with extreme measurements up to 181.9 µm. A significantly higher standard deviation (SD) was associated with IOS (37.1 µm vs. 17.7 µm, P < 0.0001). Considering angular deviations, the IOS showed slightly higher angular mean deviations (ΔANGLE) than the SPG (0.28° vs. 0.24°, P = 0.0022), with extreme measurements of up to 0.73°. The SPG SD values were significantly lower than the IOS SD values (0.14° vs. 0.04°, P < 0.0001). CONCLUSIONS: The SPG showed significantly higher 3D and angular accuracies for complete arch implant impressions, with consistent repeatability. IOS scanning revealed significantly higher extreme deviations exceeding the acceptable threshold value. Despite study limitations, SPG appears more feasible than IOS for complete-arch digital implant impressions.


Asunto(s)
Implantes Dentales , Imagenología Tridimensional , Técnica de Impresión Dental , Modelos Dentales , Diseño Asistido por Computadora
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