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1.
Res Vet Sci ; 135: 42-58, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33440244

RESUMEN

In the present research work, we propose a new antimicrobial treatment for pyoderma via cutaneous permeation of bacteriophage particles conveyed in a hydroxyethylcellulose (HEC) gel integrating ionic liquid as a permeation enhancer. Ionic liquids are highly viscous fluids constituted exclusively by ions, that are usually hydrolytically stable and promote solubilization of amphipathic molecules such as proteins, hence serving as green solvents and promoting the transdermal permeation of biomolecules. In the research effort entertained herein, the synthesis and use of choline geranate for integrating a HEC gel aiming at the structural and functional stabilization of a cocktail of isolated lytic bacteriophage particles was sought, aiming at transdermal permeation in the antimicrobial treatment of animal pyoderma. The results obtained showed a high ability of the ionic liquid in enhancing transdermal permeation of the bacteriophage particles, with concomitant high potential of the HEC gel formulation in the antimicrobial treatment of animal skin infections.


Asunto(s)
Celulosa/análogos & derivados , Colina/química , Terapia de Fagos/veterinaria , Staphylococcus intermedius/virología , Administración Cutánea , Animales , Bacteriófagos , Línea Celular , Supervivencia Celular , Celulosa/química , Perros/microbiología , Caballos/microbiología , Humanos , Líquidos Iónicos/química , Líquidos Iónicos/metabolismo , Pruebas de Mutagenicidad , Permeabilidad , Piodermia/tratamiento farmacológico , Piodermia/veterinaria , Piel/metabolismo , Solventes
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