RESUMEN
The aim of the present study was to compare the characteristics of acellular dermal matrix (ADM), small intestinal submucosa (SIS) and BioGide scaffolds with acellular vascular matrix (ACVM)0.25% humanlike collagen I (HLCI) scaffold in tissue engineering blood vessels. The ACVM0.25% HLCI scaffold was prepared and compared with ADM, SIS and BioGide scaffolds via hematoxylin and eosin (H&E) staining, Masson staining and scanning electron microscope (SEM) observations. Primary human gingival fibroblasts (HGFs) were cultured and identified. Then, the experiment was established via the seeding of HGFs on different scaffolds for 1, 4 and 7 days. The compatibility of four different scaffolds with HGFs was evaluated by H&E staining, SEM observation and Cell Counting Kit8 assay. Then, a series of experiments were conducted to evaluate water absorption capacities, mechanical abilities, the ultramicrostructure and the cytotoxicity of the four scaffolds. The ACVM0.25% HLCI scaffold was revealed to exhibit the best cell proliferation and good cell architecture. ADM and BioGide scaffolds exhibited good mechanical stability but cell proliferation was reduced when compared with the ACVM0.25% HLCI scaffold. In addition, SIS scaffolds exhibited the worst cell proliferation. The ACVM0.25% HLCI scaffold exhibited the best water absorption, followed by the SIS and BioGide scaffolds, and then the ADM scaffold. In conclusion, the ACVM0.25% HLCI scaffold has good mechanical properties as a tissue engineering scaffold and the present results suggest that it has better biological characterization when compared with other scaffold types.
Asunto(s)
Materiales Biocompatibles/química , Ingeniería de Tejidos , Andamios del Tejido/química , Materiales Biocompatibles/metabolismo , Proliferación Celular , Células Cultivadas , Colágeno/química , Colágeno Tipo I/química , Matriz Extracelular/química , Fibroblastos/citología , Fibroblastos/metabolismo , Encía/citología , Humanos , Microscopía Fluorescente , Resistencia a la TracciónRESUMEN
The effect of bioaugmentation with an acetate-type fermentation bacterium in the phylum Bacteroidetes on the anaerobic digestion of corn straw was evaluated by batch experiments. Acetobacteroides hydrogenigenes is a promising strain for bioaugmentation with relatively high growth rate, hydrogen yields and acetate tolerance, which ferments a broad spectrum of pentoses, hexoses and polyoses mainly into acetate and hydrogen. During corn straw digestion, bioaugmentation with A. hydrogenigenes led to 19-23% increase of the methane yield, with maximum of 258.1 mL/g-corn straw achieved by 10% inoculation (control, 209.3 mL/g-corn straw). Analysis of lignocellulosic composition indicated that A. hydrogenigenes could increase removal rates of cellulose and hemicelluloses in corn straw residue by 12% and 5%, respectively. Further experiment verified that the addition of A. hydrogenigenes could improve the methane yields of methyl cellulose and xylan (models for cellulose and hemicelluloses, respectively) by 16.8% and 7.0%.