RESUMEN
Bacteriophages often constitute the majority of periodontal viral communities, but phages that infect oral bacteria remain uncharacterized. Here, we present the genetic analysis of the genome of a novel siphovirus, named Siphoviridae_29632, which was isolated from a patient with periodontitis using a viral metagenomics-based approach. Among 43 predicted open reading frames (ORFs) in the genome, the viral genes encoding structural proteins were distinct from the counterparts of other viruses, although a distant homology is shared among viral morphogenesis proteins. A total of 28 predicted coding sequences had significant homology to other known phage ORF sequences. In addition, the prevalence of Siphoviridae_29632 in a cohort of patients with chronic periodontitis was 41.67%, which was significantly higher than that in the healthy group (4.55%, P < 0.001), suggesting that this virus as well as its hosts may contribute to the ecological environment favored for chronic periodontitis.
Asunto(s)
Bacteriófagos/genética , Periodontitis Crónica/virología , Bolsa Periodontal/virología , Siphoviridae/genética , Bacteriófagos/aislamiento & purificación , Bacteriófagos/patogenicidad , Periodontitis Crónica/genética , Periodontitis Crónica/microbiología , Genoma Viral/genética , Genómica , Humanos , Metagenómica , Bolsa Periodontal/genética , Bolsa Periodontal/microbiología , Filogenia , Siphoviridae/aislamiento & purificaciónRESUMEN
We describe a novel species of torque teno mini virus called TTMV-204, which was isolated from the gingival epithelium of patients with periodontitis and characterized using viral metagenomics. The sequence of the full genome is 2824 nt in length. Phylogenetic analysis and genetic analyses show classic Betatorquevirus species organization with less than 40% amino acid similarity in ORF1. The prevalence of TTMV-204 in the periodontitis patient population was 18.75% (15/80), which was higher than in periodontally healthy individuals (10.00%, 10/80). However, the difference of the TTMV-204 prevalence between two groups was not statistically significant (p = 0.115). Further investigation is required to determine whether this new virus is associated with inflammation.
Asunto(s)
Epitelio/virología , Encía/virología , Periodontitis/virología , Torque teno virus/genética , Adulto , Aminoácidos/genética , Anelloviridae/genética , Infecciones por Virus ADN/virología , ADN Viral/genética , Femenino , Genoma Viral/genética , Humanos , Masculino , Filogenia , PrevalenciaRESUMEN
A new species of torque teno mini virus, named TTMV-222, was detected in gingival tissue from periodontitis patients using a viral metagenomics method. The 2803-nucleotide genome of TTMV-222 is closely related to TTMV1-CBD279, with 62.6% overall nucleotide similarity. Genetic analyses of the new virus genome revealed a classic genomic organization but a weak identity with known sequences. The prevalence of TTMV-222 in the periodontitis group (n = 150) was significantly higher than that in the healthy group (n = 150) (p = 0.032), suggesting that the new virus may be associated with inflammation in chronic periodontitis patients. However, this finding requires further investigation.