RESUMEN
This study was investigated the role of magnesium (Mg2+) ion substituted biphasic calcium phosphate (Mg-BCP) spherical micro-scaffolds in osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs). Mg-BCP micro-scaffolds with spherical morphology were successfully prepared using in situ co-precipitation and spray drying atomization process. The in vitro cell proliferation and differentiation of hAT-MSCs were determined up to day 14. After in vitro biological tests, Mg-BCP micro-scaffolds with hAT-MSCs showed more enhanced osteogenicity than pure hAT-MSCs as control group by unique biodegradation of TCP phase and influence of substituted Mg2+ ion in biphasic nanostructure. Therefore, these results suggest that Mg-BCP micro-scaffolds promote osteogenic differentiation of hAT-MSCs.
Asunto(s)
Hidroxiapatitas/química , Magnesio/química , Células Madre Mesenquimatosas/citología , Nanosferas/química , Osteoblastos/citología , Andamios del Tejido , Adipocitos/citología , Adipocitos/fisiología , Sustitutos de Huesos/síntesis química , Diferenciación Celular/fisiología , Células Cultivadas , Diseño de Equipo , Análisis de Falla de Equipo , Humanos , Iones , Ensayo de Materiales , Células Madre Mesenquimatosas/fisiología , Nanopartículas del Metal/química , Nanopartículas del Metal/ultraestructura , Nanosferas/ultraestructura , Osteoblastos/fisiología , Osteogénesis/fisiología , Tamaño de la Partícula , Propiedades de SuperficieRESUMEN
Porous hydroxyapatite (HAp)/chitosan-alginate composite scaffolds were prepared through in situ co-precipitation and freeze-drying for bone tissue engineering. The composite scaffolds were highly porous and interconnected with a pore size of around 50-220 µm at low concentrations of HAp. As the HAp content increased, the porosity of the scaffolds decreased from 84.98 to 74.54%. An MTT assay indicates that the obtained scaffolds have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that the composite scaffold provides a strong positive effect on bone formation in vivo in mice. Furthermore, that HAp/chitosan-alginate composite scaffold has been shown to be more effective for new bone generation than chitosan-alginate scaffold.