Human odontogenic epithelial cells derived from epithelial rests of Malassez possess stem cell properties.

Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of the Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. ERM cells are unique epithelial cells that remain in periodontal tissues throughout adult life. They have a functional role in the repair/regeneration of cement or enamel. Here, we isolated odontogenic epithelial cells from ERM in the periodontal ligament, and the cells were spontaneously immortalized. Immortalized odontogenic epithelial (iOdE) cells had the ability to form spheroids and expressed stem cell-related genes. Interestingly, iOdE cells underwent osteogenic differentiation, as demonstrated by the mineralization activity in vitro in mineralization-inducing media and formation of calcification foci in iOdE cells transplanted into immunocompromised mice. These findings suggest that a cell population with features similar to stem cells exists in ERM and that this cell population has a differentiation capacity for producing calcifications in a particular microenvironment. In summary, iOdE cells will provide a convenient cell source for tissue engineering and experimental models to investigate tooth growth, differentiation, and tumorigenesis.

An in vitro study of digital impressions and three-dimensional printed models of orbital defects using mobile devices and monoscopic photogrammetry.

PURPOSE: The purpose of this study was to perform an in vitro evaluation of digital impressions using a mobile device and monoscopic photogrammetry in cases of orbital defects with undercuts. METHODS: Three 10-mm-square cubes were attached to a diagnostic cast of a patient with a right orbital defect. Still images acquired with a mobile device were used to generate facial three-dimensional (3D) data. Two types of still images were used: one was a whole face image, and the other was a defect site-focused image. For comparison, an extraoral scanner was used to obtain facial 3D data. Five dental technicians fabricated 3D printed models using additive manufacturing and measured the distances between the measurement points using a digital caliper. The discrepancy between the distances measured on the diagnostic cast of the patient and the 3D printed model was calculated. Friedman test was used to analyze the discrepancy, and the Bonferroni test was used to verify the differences between the pairs. RESULTS: Statistical significance was found with respect to the type of 3D model fabrication method. CONCLUSION: Within the limitations of this in vitro study, the results suggested that the workflow can be applied to digital impressions of the maxillofacial region.

Influence of dental abrasives on surface roughness, gloss, and color of titanium.

PURPOSE: The influence of three dental abrasives on the surface roughness, glossiness, and color of titanium (Ti) after mechanical polishing was investigated. METHODS: The specimens were divided into groups, referred to as SM, SR, and TP, to distinguish the different polishing processes. SM: rubber polishing using a rubber point containing SiC abrasive particles at 20,000 rpm, followed by gloss polishing; SR: rubber polishing using a rubber point containing Al2O3 abrasive particles at 20,000 rpm, followed by gloss polishing; TP: rubber polishing using a rubber point containing TiO2 abrasive particles at 6,000 rpm, followed by gloss polishing. Gloss polishing was carried out at 10,000 rpm using a leather buffing wheel. Measurements to evaluate the surface conditions of Ti were performed after each polishing process. RESULTS: There were no significant differences in the Ra and glossiness values between the SM and TP groups; these specimens were not as smooth and glossy as the SR group. The SM group showed relatively high values of lightness and chroma after gloss polishing. CONCLUSION: Rubber polishing with abrasives containing SiC or TiO2 particles before gloss polishing did not achieve appropriate surface conditions on Ti.