RESUMEN
We demonstrated a novel process to reconstitute a decellularized extracellular matrix (Recon-ECM) of heart and liver tissue using a combination of mechanical homogenization and enzymatic digestion. Such Recon-ECM was used as a biomaterial to produce flat or micro-patterned 2D films after crosslinking using replica molding. The mechanical properties of the resulting films were tuned by changing the type of crosslinking reagents. We also demonstrated the fabrication of mechanically robust 3D scaffolds by freeze-drying of the Recon-ECM solution. The porosity of the 3D scaffold was controlled by changing the concentration of the Recon-ECM. HepG2 cells were used to investigate the potential substrate of these engineered 2D patterned and 3D porous structures. The cell attachment, proliferation, and urea synthesis were evaluated, and the results indicate that the scaffold generated from Recon-ECM provides a biologically friendly environment for cells to grow. This method provides a new way to use decellularized ECM as a source of biomaterial to produce novel scaffolds with better controlled micro- and nano-scale structures, tunable physicochemical properties with desired biological functions.
Asunto(s)
Materiales Biocompatibles/síntesis química , Materiales Biomiméticos/síntesis química , Supervivencia Celular/efectos de los fármacos , Matriz Extracelular/química , Nanoestructuras/química , Ingeniería de Tejidos/instrumentación , Andamios del Tejido , Supervivencia Celular/fisiología , Sistema Libre de Células/química , Módulo de Elasticidad , Diseño de Equipo , Análisis de Falla de Equipo , Matriz Extracelular/ultraestructura , Células Hep G2 , Humanos , Ensayo de Materiales , Nanoestructuras/ultraestructura , Resistencia a la TracciónRESUMEN
Cell migration plays important roles in natural processes involving embryonic development, inflammation, wound healing, cancer metastasis and angiogenesis. Cell migration on various biomaterials is also believed to improve the rate of wound healing and implant therapies in the tissue-engineering field. This study measured the distance traversed, or mileage, of mouse fibroblasts on a silk fibroin surface. Fibroblasts on the fibroin surface moved with better progress during 24 h than cells on collagen or fibronectin surfaces. Results obtained by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) revealed that fibroblasts on the fibroin surface expressed transforming growth factor ß-induced protein (TGFBI), which is an extracellular matrix (ECM) protein, stronger than on other surfaces in the early cell-culture stages. These results demonstrate that the fibroin surface shows higher potential to enhance cell migration and the production of ECM than a collagen or fibronectin surface.