RESUMEN
Chinese bayberry leaf proanthocyanidins (BLPs) are Epigallocatechin gallate (EGCG) oligomers or polymers, which have a lot of health-promoting activity. The activity is closely related to their behavior during in vitro digestion, which remains unknown and hinders further investigations. To clarify the changes of BLPs during gastrointestinal digestion, further research is required. For in vitro digestion, including gastric-intestinal digestion, colon fermentation was applied. Caco-2 monolayer transportation was also applied to investigate the behavior of different BLPs with different degrees of polymerization. The trimers and the tetramers were significantly decreased during in vitro gastric-intestinal digestion resulting in a significant increase in the content of dimers. The dimers and trimers were the main compounds utilized by gut microbiota and they were assumed not to degrade through cleavage of the inflavan bond. The monomers and dimers were able to transport through the Caco-2 monolayer at a rate of 10.45% and 6.4%, respectively.
Asunto(s)
Biopolímeros/metabolismo , Ácido Gálico/análisis , Myrica/química , Proantocianidinas/metabolismo , Disponibilidad Biológica , Biopolímeros/análisis , Biopolímeros/química , Células CACO-2 , Catequina/análogos & derivados , Catequina/análisis , Catequina/química , Catequina/metabolismo , Digestión , Fermentación , Ácido Gálico/química , Ácido Gálico/metabolismo , Mucosa Gástrica/enzimología , Microbioma Gastrointestinal , Humanos , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Intestinos/enzimología , Hojas de la Planta/química , Polimerizacion , Proantocianidinas/análisis , Proantocianidinas/química , Saliva/enzimologíaRESUMEN
Polydopamine (PDA) possesses high aqueous dispersibility, strong optical absorption, and a zwitterionic property, which give it multitudes of advantages to coat light-sensitive hydrophobic curcumin (Cur) for pH-responsive release. However, PDA is formed in alkaline conditions, which hinders its potential application for alkali-sensitive curcumin coating. Here, we developed a method to prepare PDA-coated Cur nanoparticles (NPs), which reduced chemical degradation of Cur in alkaline conditions. Encapsulation efficiency and loading capacity decreased to 73.69% and 51.80%, as the time for dopamine polymerization went on. PDA could protect Cur from light-induced degradation in powder and solution forms. Controlled release and pH-responsive delivery of PDA-coated Cur were observed under stomach and intestinal conditions compared to free Cur, which resulted from the coverage and thickness of the PDA shell and the electrostatic attraction between PDA and Cur. PDA-coated Cur NPs could be a promising way for the application of Cur in the beverage and food industry.