RESUMEN
The synthesis and gene transfection efficiency of a series of amphiphilic copolymers with poly(dimethylaminoethyl methacrylate) (PDMAEMA), and poly(propylene glycol methacrylate) (PPGMA) segments is reported. The hydrophobic PPGMA interior allows a cell-sensitizing drug such as paclitaxel to be incorporated while the cationic and hydrophilic PDMAEMA corona allows the complexation of anionic DNA to form a nano-sized polyplex. These drug-encapsulated copolymers display excellent gene transfection efficiency as compared to PEI or PDMAEMA homopolymers.
Asunto(s)
ADN/química , Portadores de Fármacos/química , Metacrilatos/química , Micelas , Nylons/química , Paclitaxel/química , Polímeros/química , Animales , Células COS , Chlorocebus aethiops , ADN/metabolismo , Interacciones Hidrofóbicas e Hidrofílicas , Nanocompuestos/química , TransfecciónRESUMEN
The synthesis and gene transfection efficiency of a series of novel amphiphilic triblock copolymers with two hydrophilic poly(2-(dimethylamino)ethyl methacrylate) (DMAEMA) blocks flanking a central hydrophobic poly[(R)-3-hydroxybutyrate] (PHB) block is reported. These copolymers have significantly lower toxicity compared to the polyethyleneimine (PEI) and PDMAEMA homopolymer. Unexpectedly, the incorporation of PHB significantly improves the gene transfection efficiency as compared to PEI or PDMAEMA homopolymers.