A comprehensive review on the source, ingestion route, attachment and toxicity of microplastics/nanoplastics in human systems.

Microplastics (MPs)/nanoplastics (NPs) are widely found in the natural environment, including soil, water and the atmosphere, which are essential for human survival. In the recent years, there has been a growing concern about the potential impact of MPs/NPs on human health. Due to the increasing interest in this research and the limited number of studies related to the health effects of MPs/NPs on humans, it is necessary to conduct a systematic assessment and review of their potentially toxic effects on human organs and tissues. Humans can be exposed to microplastics through ingestion, inhalation and dermal contact, however, ingestion and inhalation are considered as the primary routes. The ingested MPs/NPs mainly consist of plastic particles with a particle size ranging from 0.1 to 1 µm, that distribute across various tissues and organs within the body, which in turn have a certain impact on the nine major systems of the human body, especially the digestive system and respiratory system, which are closely related to the intake pathway of MPs/NPs. The harmful effects caused by MPs/NPs primarily occur through potential toxic mechanisms such as induction of oxidative stress, generation of inflammatory responses, alteration of lipid metabolism or energy metabolism or expression of related functional factors. This review can help people to systematically understand the hazards of MPs/NPs and related toxicity mechanisms from the level of nine biological systems. It allows MPs/NPs pollution to be emphasized, and it is also hoped that research on their toxic effects will be strengthened in the future.

A comparative study on the adsorption behavior of pesticides by pristine and aged microplastics from agricultural polyethylene soil films.

Compared with pristine agricultural polyethylene (PE) soil films microplastics (MPs), aged agricultural polyethylene (APE) soil films MPs have a rougher surface, more cracks and have some oxygen-containing functional groups that makes them adsorb organic pollutants, such as pesticides more easily. This may be more harmful to human beings than marine MPs as the agricultural soil films are closer to our living environment. But few works focused on the adsorption of pesticides on pristine or aged agricultural polyethylene soil films MPs. In order to promote the risk assessment of co-exposure of pesticides and agricultural polyethylene soil films MPs, a comparative study on the adsorption behavior and mechanism of four pesticides (carbendazim, diflubenzuron, malathion, difenoconazole) by pristine PE MPs and APE MPs were carried out in this paper. The results showed microcracks and surface oxidation observed on APE MPs. The adsorption kinetics and isotherm models indicated that the adsorption capacity of APE MPs was higher than that of PE MPs, which attribute to the larger surface area of APE MPs. The adsorption capacities of pesticides on APE MPs were positively correlated with LogKow (Water octanol partition coefficient) values of these four pesticides, showed the hydrophobic partitioning played the most important part in the adsorption, but also some H-bonding between secondary amines in the molecular of diflubenzuron and polar O-containing functional groups on APE MPs may be formed. And electrostatic forces or interactions are not the determining factor for these pesticides adsorption behavior of PE MPs, and the effect of pH is mainly driven by changes in sorbate properties rather than changes in surface properties of MPs. The results presented herein show the APE MPs can be a better vector of most hydrophobic pesticides than pristine MPs in the agricultural field, and more attention should be paid to the problem of films and pesticides residue in farmland soil.

Fluorescence-tagged amphiphilic brush copolymer encapsulated Gd2O3 core-shell nanostructures for enhanced T 1 contrast effect and fluorescent imaging.

To obtain suitable T 1 contrast agents for magnetic resonance imaging (MRI) application, aqueous Gd2O3 nanoparticles (NPs) with high longitudinal relativity (r 1) are demanded. High quality Gd2O3 NPs are usually synthesized through a non-hydrolytic route which requires post-synthetic modification to render the NPs water soluble. The current challenge is to obtain aqueous Gd2O3 NPs with high colloidal stability and enhanced r 1 relaxivity. To overcome this challenge, fluorescence-tagged amphiphilic brush copolymer (AFCP) encapsulated Gd2O3 NPs were proposed as suitable T 1 contrast agents. Such a coating layer provided (i) superior aqueous stability, (ii) biocompatibility, as well as (iii) multi-modality (conjugation with fluorescence dye). The polymeric coating layer thickness was simply adjusted by varying the phase-transfer parameters. By reducing the coating thickness, i.e. the distance between the paramagnetic centre and surrounding water protons, the r 1 relaxivity could be enhanced. In contrast, a thicker polymeric layer coating prevents Gd(3+) ions leakage, thus improving its biocompatibility. Therefore, it is important to strike a balance between the biocompatibility and the r 1 relaxivity behaviour. Lastly, by conjugating fluorescence moiety, an additional imaging modality was enabled, as demonstrated from the cell-labelling experiment.

Cerebral neurotoxicity of amino-modified polystyrene nanoplastics in mice and the protective effects of functional food Camellia pollen.

Accumulating evidence suggests that nanoplastics contribute to an increased risk of brain damage, however, the precise underlying mechanisms remain unclear. Here, we subjected mice to long-term exposure to amino-modified polystyrene nanoplastics (APS-NPs). These nanoplastics were detected in the mouse brain; coupled with the observed upregulation of Alzheimer's disease-associated genes (APP and MAPT). To further explore nanoplastic damage mechanisms and the corresponding protective strategies against these mechanisms in vitro, we used hCMEC/D3 and HT22 cells. Results showed that APS-NPs disrupted tight junction proteins (Occludin and ZO-1) via TLR2/MMP9 axis, resulting in blood-brain barrier permeation; this was significantly mitigated by functional food Camellia pollen treatment. APS-NPs initiated iNOS and nNOS upregulation within neurons resulting in Sirtuin 1 deacetylase inactivation and CBP acetyltransferase stimulation, ultimately leading to Ac-Tau formation. This process was attenuated by Camellia pollen, which also ameliorated the APS-NPs-induced neuronal apoptosis mediated by the p53/Bax/Bcl-2 axis. Network pharmacology analysis of Camellia pollen offered a further theoretical understanding of its potential applications in preventing and treating nervous system disorders, such as Alzheimer's disease. This study established that Camellia pollen protects the brain against APS-NPs-mediated blood-brain barrier damage and alleviates neuronal apoptosis and Alzheimer's disease-like neurotoxicity. This study elucidates the mechanisms underlying polystyrene-induced brain damage and can be used to inform future prevention and treatment strategies.

Optimize lettuce washing methods to reduce the risk of microplastics ingestion: The evidence from microplastics residues on the surface of lettuce leaves and in the lettuce washing wastewater.

Airborne microplastics have been identified as an emerging contaminant that can adhere to the surface of leafy vegetables, and if not completely removed by washing, there is a high risk that human consumption of these plastics may cause harm to humans. In this study, we simulated atmospheric pollution by spraying microplastic particles (MPs) with particle sizes of 100 nm and 500 nm to determine whether MPs particles would adhere to the lettuce surface and whether different common cleaning methods (water rinsing, ultrasonic vibration cleaning, and edible detergent cleaning) would be effective in removing MPs from the leaf surfaces. We scanned the leaf surface with a laser confocal microscope and examined the wash water with a flow cytometer and found that simple rinsing was not effective in removing plastic particles from lettuce leaves. In comparison, ultrasonic vibratory cleaning showed a better efficiency, 4 times higher than more MPs being washed from the leaves. The most effective method was detergent washing, with the washing efficiency increased by 6.9 times. Ultrasonic vibrations can partially break the chemical bonds between MPs and plant surfaces, and detergents' surfactants can enhance MPs' hydrophilicity. MPs with a particle size of 100 nm were more difficult to clean than those with a particle size of 500 nm. This finding has important implications for the interaction of MPs contamination with vegetables and the cleaning of vegetables.

Uptake, transport and accumulation of micro- and nano-plastics in terrestrial plants and health risk associated with their transfer to food chain - A mini review.

Waste plastics enter the environment (water, soil, and atmosphere) and degrade into micro- and nano-plastics (MNPs) through physical, chemical, or biological processes. MNPs are ubiquitous in the environment and inevitably interact with terrestrial plants. Terrestrial plants have become important potential sinks, and subsequently, the sources of MNPs. At present, many studies have reported the effects of MNPs on plant physiology, biochemistry, and their phototoxicity. However, the source, detection method, and the absorption process of MNPs in terrestrial plants have not been systematically studied. In order to better understand the continuous process of MNPs entering terrestrial plants, this review introduces the sources and analysis methods of MNPs in terrestrial plants. The uptake pathways of MNPs in terrestrial plants and their influencing factors were systematically summarized. Meanwhile, the transport pathways and the accumulation of MNPs in different plant organs (roots, stems, leaves, calyxes, and fruits) were explored. Finally, the transfer of MNPs through food chains to humans and their health risks were discussed. The aim of this work is to provide significant theoretical knowledge to understand the uptake, transport, and accumulation of MNPs in terrestrial plants and the potential health risks associated with their transfer to humans through food chain.

Treatment of a synthetic decanted oily seawater in a pilot-scale hollow fiber membrane filtration process: Experimental investigation.

This study investigates the performance of a pilot-scale submerged hollow fiber (HF) ultrafiltration (UF) polytetrafluoroethylene (PTFE) membrane filtration system for the treatment of two different types of oily seawater (i.e., seawater contaminated with light and heavy crude oil). The effects of membrane flux and aeration flow rate on membrane performance and the removal efficiency of different fractions of hydrocarbon, including polycyclic aromatic hydrocarbons (PAHs) were examined. The results for both heavy and light crude oil contaminated wastewater reveal that total petroleum hydrocarbon (TPH) removal efficiency of more than 91% was achieved. This research paper determined the optimal operational parameters for an HF membrane filtration system to obtain a good TPH removal efficiency. This system can easily be upscaled and placed on a barge to treat oily wastewater generated from marine oil spills, which can significantly improve the oil spill response capacity.

Comparative analysis of the sorption behaviors and mechanisms of amide herbicides on biodegradable and nondegradable microplastics derived from agricultural plastic products.

Coexisting of microplastics (MPs) and residual herbicides has received substantial attention due to concerns about the pollutant vector effect. Here, the widely used amide herbicides were examined for their sorption behaviors on the priority biodegradable and nondegradable MPs identified in intensive agriculture. The fitting results indicated that the interactions between napropamide (Nap)/acetochlor (Ace) and the MPs, i.e., poly (butyleneadipate-co-terephthalate) microplastic (PBATM), polyethylene microplastic (PEM), and polypropylene microplastic (PPM), may be dominated by hydrophobic absorptive partitioning on the heterogeneous surfaces. Additionally, chemisorption cannot be ignored for the sorption of Nap/Ace on the biodegradable MPs. The sorption capacities of Nap/Ace on the MPs followed the order of PBATM > PEM > PPM. The differences in sorption capacity which varied by the MP colors were not significant. The hydrophobicity of the herbicides and the MPs, the rubber regions, surface O-functional groups, benzene ring structures and large specific surface area of the biodegradable MPs played key roles in the better performance in sorbing amide herbicides. Moreover, MPs, especially biodegradable MPs, might lead to a higher vector effect for residual amide herbicides than some other common environmental media. This study may provide baseline insights into the great potential of biodegradable MPs to serve as carriers of residual amide herbicides in intensive agrosystems.

A comparative study on the adsorption behavior and mechanism of pesticides on agricultural film microplastics and straw degradation products.

Straw will degrade into segment, powder and crystalline cellulose, while the agricultural film will degrade into microplastics (MPs) in farmland soils. The specific surface area of these micro-particles increases and many new functional groups are formed in the degradation process, which can be a good vector of pesticides. To more accurately and truly analyze the risk of main imported substances and their degradation products against pollutants in soil, the adsorption behavior and mechanism of four commonly used pesticides on aged polyethylene microplastics (APE), wheat straw segment (WSS), wheat straw powder (WSP), and straw crystalline cellulose (SCC) were analyzed and compared through batch adsorption experiments and infrared spectrum. The adsorption kinetics of four pesticides on MPs and straw degradation products tended to be pseudo-second-order kinetics; the adsorption isotherms of pesticides on APE and SCC tended to fit the Freundlich model, while on WSP and WSS tended to fit the Langmuir model. The adsorption was a spontaneous endothermic increase process, suggesting that the main adsorption force of pesticides on MPs and straw degradation products was hydrophobic diffusion. The adsorption of pesticides against WSP and WSS still had a certain π-π conjugation and electrostatic interaction. And the adsorption amount on the straw degradation products followed the order of WSP > WSS > APE > SCC, presumably related to the specific surface area and pore volume of the adsorbent. As WSP, WSS could adsorb more pesticides, the straw returning to the field can be used for slow-release of pesticides to reduce the dosage of pesticides.

Adsorption of three pesticides on polyethylene microplastics in aqueous solutions: Kinetics, isotherms, thermodynamics, and molecular dynamics simulation.

Microplastics could act as a carrier for pesticides in the water environment and pose a potential risk. This study mainly investigated the effects of reaction time, microplastics dosages, pH, and NaCl salinity on the adsorption characteristics of three pesticides (Imidacloprid, Buprofezin, Difenoconazole) on polyethylene (PE) microplastics in aqueous solution. The results showed that high pH and low NaCl salinity were conducive to the adsorption. The adsorption data were well fitted by the Freundlich isotherm model and the pseudo-first-order kinetics, indicating that it was mainly controlled by physical function. The adsorption capacity of three pesticides on PE microplastics followed the order of Difenoconazole > Buprofezin > Imidacloprid. The thermodynamic study indicated the adsorption of all pesticides as spontaneous and exothermic processes, and the elevated temperature was favorable to the adsorption. SEM-EDS and FTIR results verified that pesticides were adsorbed on the microplastics but the adsorption process was mainly controlled by intermolecular Van Der Waals Force and the microporous filling mechanism. Grand Canonical Monte Carlo (GCMC) and molecular dynamics (MD) simulation results indicated that surface adsorption was the exclusive mechanism for the adsorption of pesticides on microplastics, and the final adsorption configurations revealed that there were complex interactions between the pesticide molecules and the C, H atoms in PE molecules. The results of this study illustrated that PE microplastics are potential carriers for pesticides in the water environment.

Adsorption of tetracyclines onto polyethylene microplastics: A combined study of experiment and molecular dynamics simulation.

Microplastics are a kind of new organic pollutant in the environment. In this study, the adsorption of tetracyclines (TCs), including tetracycline hydrochloride (TC), chlortetracycline hydrochloride (CTC) and oxytetracycline hydrochloride (OTC) onto polyethylene (PE) microplastics in aqueous solutions were investigated. The mechanism of the adsorption behavior was preliminarily explored by adsorption kinetics, isotherms, and thermodynamics, in combination with scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). In addition, molecular dynamics (MD) simulation was applied to investigate the adsorption processes of TCs on PE at a molecular level. It was found that the adsorption behaviors of TCs reached an equilibrium state within 30 h. The experimental data showed that adsorption capacities of TCs onto PE were as follows: OTC (64.40 ± 2.38 µg/g)>CTC (63.36 ± 4.92 µg/g)>TC (53.52 ± 3.43 µg/g). TC sorption onto PE increased with pH, peaking at around pH 6 and then decreased. The increase of ionic strength in the solution led to the reduced adsorption capacity of TC onto PE. The results indicated that the experimental data were well fitted by the pseudo-second-order model and the Freundlich isotherm model, indicating both monolayer and multilayer coverage of TCs onto the surface of PE. The results of MD simulation showed that PE can effectively adsorb the TCs molecule mainly through non-bond interactions, and PE exhibited the highest affinity for CTC and OTC, followed by TC.

Adsorption behavior and mechanism of five pesticides on microplastics from agricultural polyethylene films.

Polyethylene (PE) agricultural soil films are easily embrittled and decomposed to microplastics (MPs) in environment. As widely used pesticides in vegetable farmland, carbendazim, dipterex, diflubenzuron, malathion, difenoconazole have potential environmental and human safety risks. They are often coexisting with MPs in the environment, and may cause consequential pollution to the ecosystem. Studying the adsorption behavior between pesticides and PE agricultural soil films MPs would be helpful for the risk assessment of co-exposure of pesticides and MPs. Herein, a systematic study on batch adsorption experiments was performed to determine the adsorption process of pesticides on MPs, the environmental factors on adsorption capacity were evaluated, and the adsorption mechanisms were discussed. Results suggested that all these five pesticides can adsorb on MPs, especially for diflubenzuron and difenoconazole. The adsorption kinetics and isotherm fitted to the Pseudo-second-order and Freundlich model, respectively, indicating that besides the adsorption onto surface sites, mass transfer and intraparticle diffusion were involved in the adsorption process, and the adsorption process was mostly controlled by physical and chemical interactions. The adsorption amounts of 5 pesticides on PE MPs follow the order of DIF > DIFE > MAL > CAR > DIP with KF correlated positively with octanol-water partition coefficients (LogKow). The thermodynamic study indicates the adsorption of all pesticides as spontaneous and exothermic processes. The results of this study illustrated that PE MPs can be a good carrier of pesticides in agricultural field.