Conjugated Polymer Nanoparticles for Tumor Theranostics.

Water-dispersible conjugated polymer nanoparticles (CPNs) have demonstrated great capabilities in biological applications, such as in vitro cell/subcellular imaging and biosensing, or in vivo tissue imaging and disease treatment. In this review, we summarized the recent advances of CPNs used for tumor imaging and treatment during the past five years. CPNs with different structures, which have been applied to in vivo solid tumor imaging (fluorescence, photoacoustic, and dual-modal) and treatment (phototherapy, drug carriers, and synergistic therapy), are discussed in detail. We also demonstrated the potential of CPNs as cancer theranostic nanoplatforms. Finally, we discussed current challenges and outlooks in this field.

Semiconducting polymer dots as fluorescent probes for in vitro biosensing.

Semiconducting polymer dots (Pdots) have emerged as novel fluorescent probes with excellent characteristics, such as ultrahigh molar extinction coefficient, easy tunable absorption and emission bands, high brightness, and excellent photostability. Combined with good biocompatibility properties, much effort has been devoted to Pdots for in vivo biological imaging and therapy applications, such as deep-tissue fluorescent imaging, photodynamic therapy, photothermal therapy, and nanocarriers of genes or chemical drugs. Many reviews have been presented in these fields. On the other hand, a large number of studies employing Pdots for in vitro biosensing applications have been reported during the past few years, and there are barely any relevant reports to summarize the progress in this area. Hence, it is necessary to review these studies to promote the comprehensive application of Pdots. Herein, we introduce the properties and functionalization of Pdots, and systematically summarize the progress in the in vitro applications of Pdots, including the detection of DNAs, microRNAs, proteins, enzymatic activity, and some biological small molecules and ions. Finally, we share our perspectives on the future direction of this field.

Recent Advances of NIR-II Emissive Semiconducting Polymer Dots for In Vivo Tumor Fluorescence Imaging and Theranostics.

Accurate diagnosis and treatment of tumors, one of the top global health problems, has always been the research focus of scientists and doctors. Near-infrared (NIR) emissive semiconducting polymers dots (Pdots) have demonstrated bright prospects in field of in vivo tumor fluorescence imaging owing to some of their intrinsic advantages, including good water-dispersibility, facile surface-functionalization, easily tunable optical properties, and good biocompatibility. During recent years, much effort has been devoted to developing Pdots with emission bands located in the second near-infrared (NIR-II, 1000-1700 nm) region, which hold great advantages of higher spatial resolution, better signal-to-background ratios (SBR), and deeper tissue penetration for solid-tumor imaging in comparison with the visible region (400-680 nm) and the first near-infrared (NIR-I, 680-900 nm) window, by virtue of the reduced tissue autofluorescence, minimal photon scattering, and low photon absorption. In this review, we mainly summarize the latest advances of NIR-II emissive semiconducting Pdots for in vivo tumor fluorescence imaging, including molecular engineering to improve the fluorescence quantum yields and surface functionalization to elevate the tumor-targeting capability. We also present several NIR-II theranostic Pdots used for integrated tumor fluorescence diagnosis and photothermal/photodynamic therapy. Finally, we give our perspectives on future developments in this field.

Spatiotemporal control of cell-cell reversible interactions using molecular engineering.

Manipulation of cell-cell interactions has potential applications in basic research and cell-based therapy. Herein, using a combination of metabolic glycan labelling and bio-orthogonal click reaction, we engineer cell membranes with ß-cyclodextrin and subsequently manipulate cell behaviours via photo-responsive host-guest recognition. With this methodology, we demonstrate reversible manipulation of cell assembly and disassembly. The method enables light-controllable reversible assembly of cell-cell adhesion, in contrast with previously reported irreversible effects, in which altered structure could not be reused. We also illustrate the utility of the method by designing a cell-based therapy. Peripheral blood mononuclear cells modified with aptamer are effectively redirected towards target cells, resulting in enhanced cell apoptosis. Our approach allows precise control of reversible cell-cell interactions and we expect that it will promote further developments of cell-based therapy.

Detection of telomerase on upconversion nanoparticle modified cellulose paper.

Herein we report a convenient and sensitive method for the detection of telomerase activity based on upconversion nanoparticle (UCNP) modified cellulose paper. Compared with many solution-phase systems, this paper chip is more stable and easily stores the test results. What's more, the low background fluorescence of the UCNPs increases the sensitivity of this method, and the low telomerase levels in different cell lines can clearly be discriminated by the naked eye.

Recent progress in nanosensors for sensitive detection of biomolecules.

Developing sensitive, rapid, and cost-effective methods for detection of biomolecules is important for both clinical and numerous non-clinical applications. During the last two decades, functional nanomaterials with unique physical and chemical properties have provided significant advantages for biological detection. In this feature article, we introduce recent progress in nanobiosensor development by exploiting the optical, electrical and catalytic properties of a range of nanomaterials, with a focus on gold nanoparticles, carbon nanotubes, graphene and carbon dots. In addition, the perspectives on future opportunities and unsolved challenges are also discussed.

3D graphene oxide-polymer hydrogel: near-infrared light-triggered active scaffold for reversible cell capture and on-demand release.

An active cell scaffold based on a graphene-polymer hydrogel has been successfully fabricated. The macroporous hydrogel can efficiently capture cells not only through the bioadhesive ligand RGD but also through on-demand release of cells with an NIR light stimulus. The latter process shows better dynamic control over cells than traditional passive-hydrogel-based cell depots.