Role of HIF-2α/NF-κB pathway in mechanical stress-induced temporomandibular joint osteoarthritis.

OBJECTIVES: Many activities overload temporomandibular joint (TMJ) and cause mandibular condylar cartilage (MCC) degradation by inducing the expression of hypoxia-inducible factor-2α (HIF-2α). Although NF-κB signaling pathway has been reported to induce HIF-2α expression, the underlying mechanisms need to be verified. The aim was to investigate the effects of NF-κB/HIF-2α on MCC degradation induced by mechanical stress and the regulatory mechanism of NF-κB in the HIF-2α pathway. METHODS: Chondrocytes were subjected to cyclic compressive forces in a hypoxic environment. Western blotting was used to test the effects of stress on the expression of NF-κB and HIF-2α. HIF-2α siRNA and shRNA were constructed and transfected into MCC cells in vitro and in vivo to inhibit HIF-2α expression. To test the regulatory effect of the NF-κB pathway on HIF-2α, siRNA p65 was transfected into MCC. RESULTS: The results showed that mechanical stress could cause cartilage degradation and significantly increased the expression of NF-κB, HIF-2α, and downstream degradation factors (MMP13 and ADAMTs-4). Blockade of HIF-2α decreased cartilage degradation and related degradation factors. Suppression of p65 significantly decreased the expression of HIF-2α. CONCLUSIONS: Our results indicated that the upstream NF-κB pathway exerted a regulatory effect on HIF-2α in the degradation of MCC induced by stress.

The comparative evaluation of transcrestal and lateral sinus floor elevation in sites with residual bone height ≤6 mm: A two-year prospective randomized study.

OBJECTIVES: To compare clinical and radiographic outcomes between transcrestal sinus floor elevation (TSFE) and lateral sinus floor elevation (LSFE) approaches of simultaneous implant placement in atrophic maxilla. MATERIALS AND METHODS: Patients with a residual bone height (RBH) ≤6 mm were enrolled and randomly assigned to TSFE and LSFE groups. Patients in both groups simultaneously underwent sinus floor elevation with bovine-derived xenograft and implant placement. Clinical and radiographic results were evaluated immediately after surgery and after 6, 12, 18, and 24 months. The endo-sinus bone gain (ESBG), apical implant bone height (ABH), endo-sinus bone-implant contact rate (EBICR), and crestal bone level (CBL) were assessed using panoramic radiographs. RESULTS: Forty-one implants (TSFE: 21, LSFE: 20) were placed in cases with a mean RBH of 3.77 ± 1.16 mm. All implants obtained clinical success and satisfactory ESBG at 24 months. No significant differences were found in ESBG and ABH between two groups immediately after surgery, but LSFE group showed significantly higher values than TSFE group thereafter. Grafts in TSFE group reached stability 6 months earlier than that in LSFE group. In both groups, EBICR was almost 100%, and CBL showed no detectable changes. CONCLUSIONS: LSFE can achieve higher ESBG 2 years after surgery. Otherwise, TSFE could be an alternative to LSFE, when the access for lateral window preparation is limited. Both approaches were highly predictable for RBH ≤6 mm during 24-month observation period for the implants placed simultaneously.

Time to Safeguard the Future Generations from the Omnipresent Microplastics.

Microplastics (MPs) as a ubiquitous environmental pollutant have drawn growing attention, and it is concerning that children are more sensitive to MPs than adults. Unfortunately, information about the link between children and MPs is insufficient. Therefore, we review the sources and exposure routes of children to MPs and collect evidence for the potential risks. Children can ingest and/or inhale MPs derived from various foodstuffs and plastic products. Despite the limited knowledge on the toxicity to humans, current studies have proved the accumulation and translocation of MPs in different tissues and organs. Main damages including cytotoxicity, neurotoxicity, and immunotoxicity can be caused by pristine polymers and/or co-contaminants. There is much more to be understood about MPs, especially their health effects, and this study has made it clear that it is time to protect our future generations from the threat of MPs.

Influence of Microplastics on the Mobility, Bioavailability, and Toxicity of Heavy Metals: A Review.

Microplastics (MPs) can pose ecological risk to the environment and have the potential to negatively affect human health, raising serious public concerns. It is recognized that MPs could act as a vector for various environmental pollutants including heavy metals and potentially influencing their mobility, fate, and bioavailabilty in the environment. However, knowledge on the mechanisms underpinning the interaction processes between MPs and heavy metals is far from clear. This review discusses the effects of MPs on the adsorption/desorption, speciation and bioavailability, and toxicity of various heavy metals. The present review also systematically identifies the environmental factors (e.g., pH, ionic strength, and organic matters) that could affect their interaction processes. This work aims to establish a meaningful perspective for a comprehensive understanding of the indirect ecological risks of MPs as vectors for contaminants. The work also provides a reference for the development of better regulatory strategies in mitigating the negative effects caused by the co-existence of MPs and heavy metals.

Microplastics in waters and soils: Occurrence, analytical methods and ecotoxicological effects.

Microplastics (MPs) are ubiquitous in the environment and more abundant in the marine environment. Consequently, increasing focus has been put on MPs in oceans and seas, while little importance has been attached to their presence in freshwaters and soils. Therefore, this paper aimed to provide a comprehensive review of the occurrence, analysis and ecotoxicology of MPs. The abundance and distribution of MPs in several typical freshwater systems of China were summarized. It suggested that the surface water of Poyang Lake contained the highest concentration of 34 items/L MPs among all the 8 freshwater systems, and the content of MPs in sediments were higher than that of the surface water. Net-based zooplankton sampling methods are the most frequently utilized sampling methods for MPs, and density separation, elutriation and digestion are three major pretreatment methods. Fourier transform infrared spectroscopy, Raman spectroscopy and pyrolysis-gas chromatography coupled to mass spectrometry are often used to identify the polymer types of MPs. Besides, MPs might damage the digestive tract of various organisms and negatively inhibit their growth, feeding and reproduction. The ways of human exposure to MPs are by ingestion, inhalation and dermal exposure, digestive and respiratory system might be adversely influenced. However, potential health risks of MPs to humans are remained insufficiently researched. Overall, by showing the presence of MPs in freshwaters and soils as well as possible ecotoxicological effects on the environment and humans, this paper provided a framework for future research in this field.

Enhanced enzymatic hydrolysis of wheat straw by two-step pretreatment combining alkalization and adsorption.

Wheat straws (WS) were pretreated by a two-step pretreatment for enhanced enzymatic hydrolysis. The raw wheat straw (RWS) was firstly soaked in microwave-assisted NaOH solution at the presence of a mixed solution of H2O2 and hexadecyl trimethyl ammonium chloride (HTAC), and the residue was put into methyl orange (MO) solution to obtain the final substrate for enzymatic hydrolysis. It was interesting to find that the WS after the first step of the pretreatment could effectively remove MO in the second step of the pretreatment with the highest removal efficiency of 98.86% at the MO concentration of 25 mg/L and at the adsorbent-adsorbate ratio of 0.8%. Meanwhile, the enzymatic hydrolysis efficiency of the WS that was pretreated by the two steps was higher than that of the RWS as well as the first-step-pretreated WS. Enzymatic hydrolysis experiment showed that the glucose concentration after 48-h hydrolysis was 4.85 g/L at the solid loading of 5%, compared with the RWS of 0.90 g/L and the first-step-pretreated WS of 4.33 g/L. This suggested that the two-step pretreatment method could not only make WS as a good adsorbent but also a more biodegradable lignocellulosic material. Characterization analysis showed that the specific surface area and the cellulose content of the WS were both increased after the two-step pretreatment, indicating that the pretreatment method was significant to properly utilizing WS as biosorbent and biofuel. Besides, a possible mechanism for the effect of the pretreatment on the WS was proposed.

Trend of Cadherin-11 expression and its impact on cartilage degradation in the temporomandibular joints of guinea pigs with spontaneous osteoarthritis.

BACKGROUND: This study aims to investigate spatial and temporal changes in cadherin-11 (CAD-11) expression and their effects on cartilage degeneration in the temporomandibular joint (TMJ) of guinea pigs with spontaneous osteoarthritis (OA). METHODS: Dunkin-Hartley (DH) and Bristol strain 2 (BS2) guinea pigs at ages of 1, 3, 6, 9, and 12 months were categorized into two groups and analyzed. The bilateral TMJ condyles of DH and BS2 guinea pigs were harvested and fixed. The distribution and expression profiles of CAD-11, collagen type II, and matrix metalloproteinase 3 (MMP-3) were detected by immunohistological assays. Histological micrographs of the condyle cartilage were obtained and analyzed. RESULTS: Osteoarthritis can be spontaneously induced by mechanical stress in DH guinea pigs. The main histopathological changes in the TMJ structure and increased expression of MMP-3 occurred within 6-9 months of ages in DH guinea pigs with spontaneous OA. By contrast, minimal to mild cartilage degradations were observed in the TMJ of BS2 guinea pigs even at the age of 12 months. From as early as 3 months of age, the expression levels of CAD-11 were upregulated in the TMJ of DH guinea pigs compared with those in BS2 animals. CAD-11 expression differed between the two groups at 12 months of age. CONCLUSIONS: Increased CAD-11 expression within cartilage is associated with the development and progression of OA between the two strains of guinea pigs. Therefore, CAD-11 expression in TMJ could be an important predisposing factor for the development of spontaneous OA.

Hydrostatic Compress Force Enhances the Viability and Decreases the Apoptosis of Condylar Chondrocytes through Integrin-FAK-ERK/PI3K Pathway.

Reduced mechanical stimuli in many pathological cases, such as hemimastication and limited masticatory movements, can significantly affect the metabolic activity of mandibular condylar chondrocytes and the growth of mandibles. However, the molecular mechanisms for these phenomena remain unclear. In this study, we hypothesized that integrin-focal adhesion kinase (FAK)-ERK (extracellular signal-regulated kinase)/PI3K (phosphatidylinositol-3-kinase) signaling pathway mediated the cellular response of condylar chondrocytes to mechanical loading. Primary condylar chondrocytes were exposed to hydrostatic compressive forces (HCFs) of different magnitudes (0, 50, 100, 150, 200, and 250 kPa) for 2 h. We measured the viability, morphology, and apoptosis of the chondrocytes with different treatments as well as the gene, protein expression, and phosphorylation of mechanosensitivity-related molecules, such as integrin α2, integrin α5, integrin ß1, FAK, ERK, and PI3K. HCFs could significantly increase the viability and surface area of condylar chondrocytes and decrease their apoptosis in a dose-dependent manner. HCF of 250 kPa resulted in a 1.51 ± 0.02-fold increase of cell viability and reduced the ratio of apoptotic cells from 18.10% ± 0.56% to 7.30% ± 1.43%. HCFs could significantly enhance the mRNA and protein expression of integrin α2, integrin α5, and integrin ß1 in a dose-dependent manner, but not ERK1, ERK2, or PI3K. Instead, HCF could significantly increase phosphorylation levels of FAK, ERK1/2, and PI3K in a dose-dependent manner. Cilengitide, the potent integrin inhibitor, could dose-dependently block such effects of HCFs. HCFs enhances the viability and decreases the apoptosis of condylar chondrocytes through the integrin-FAK-ERK/PI3K pathway.

Correction of the transverse discrepancy-induced spontaneous mandibular protrusion in Class II Division 1 adolescent patients.

BACKGROUND: A Class Il malocclusion is the most frequent sagittal skeletal disharmony presenting for orthodontic treatment. A transverse interarch discrepancy ITID) may be considered as a possible functional cause of a Class 11 relationship. OBJECTIVE: The purpose of the present study was to determine transverse interarch width dimensions before and after orthodontic therapy and their possible relationship with increased mandibular projection following treatment. METHODS: The sample included 40 adolescent patients who were divided into two groups, one possessing and one without a transverse discrepancy. Interarch width differences (including ICWD, IPWD, IMWD, IAWD) were measured before and after treatment, and Pogonion (Pog) to Nasion (NJ perpendicular was similarly measured in each group. RESULTS: The differences in arch and alveolar width dimensions between the two groups (including ICWD, IPWDI, IPWDII, IMWD, IAWD) before treatment were statistically significant (p < 0.05). A comparison of Pog to N perpendicular between the two groups showed that mandibular protrusion after treatment in the transverse discrepancy group was 2.6 ± 1.3 mm, while mandibular protrusion after treatment in the group without a transverse discrepancy was 0.6 ±0.3 mm. The statistical comparison showed that the differences were significant (p < 0.01). CONCLUSION: A transverse interarch discrepancy may have a functional relationship with mandible retrusion. If a transverse discrepancy is corrected via orthodontic treatment, the mandible may spontaneously protrude.

Effective On-Line Performance Modulation and Efficient Continuous Preparation of Ultra-Long Twisted and Coiled Polymer Artificial Muscles for Engineering Applications.

Artificial muscle is a kind of thread-like actuator that can produce contractile strain, generate force, and output mechanical work under external stimulations to imitate the functions and achieve the performances of biological muscles. It can be used to actuate various bionic soft robots and has broad application prospects. The electrically controlled twisted and coiled polymer (TCP) artificial muscles, with the advantages of high power density, large stroke and low driving voltage, while also being electrolyte free, are the most practical. However, the relationship between the muscle performances and its preparation parameters is not very clear yet, and the complete procedure of designing and preparing TCP muscles according to actual needs has not been established. Besides, current preparation approaches are very time-consuming and cannot make ultra-long TCP muscles. These problems greatly limit wide applications of TCP artificial muscles. In this study, we studied and built the relationship between the actuating performances of TCP muscles and their preparation parameters, so that suitable TCP muscles can be easily designed and prepared according to actual requirements. Moreover, an efficient preparation method integrating one-step annealing technique has been developed to realize on-line performance modulation and continuous fabrication of ultra-long TCP muscles. By graphically assembling long muscles on heat-resist films, we designed and produced a series of fancy soft robots (butterfly, flower, starfish), which can perform various bionic movements and complete specific tasks. This work has achieved efficient on-demand preparation and large-scale assembly of ultra-long TCP muscles, laying solid foundations for their engineering applications in soft robot field.

Sulfoxide-containing polymers conjugated prodrug micelles with enhanced anticancer activity and reduced intestinal toxicity.

Poly(ethylene glycol) (PEG) is widely utilized as a hydrophilic coating to extend the circulation time and improve the tumor accumulation of polymeric micelles. Nonetheless, PEGylated micelles often activate complement proteins, leading to accelerated blood clearance and negatively impacting drug efficacy and safety. Here, we have crafted amphiphilic block copolymers that merge hydrophilic sulfoxide-containing polymers (psulfoxides) with the hydrophobic drug 7-ethyl-10-hydroxylcamptothecin (SN38) into drug-conjugate micelles. Our findings show that the specific variant, PMSEA-PSN38 micelles, remarkably reduce protein fouling, prolong blood circulation, and improve intratumoral accumulation, culminating in significantly increased anti-cancer efficacy compared with PEG-PSN38 counterpart. Additionally, PMSEA-PSN38 micelles effectively inhibit complement activation, mitigate leukocyte uptake, and attenuate hyperactivation of inflammatory cells, diminishing their ability to stimulate tumor metastasis and cause inflammation. As a result, PMSEA-PSN38 micelles show exceptional promise in the realm of anti-metastasis and significantly abate SN38-induced intestinal toxicity. This study underscores the promising role of psulfoxides as viable PEG substitutes in the design of polymeric micelles for efficacious anti-cancer drug delivery.

Remote Activation of M2 Macrophage Polarization via Magneto-Mechanical Stimulation To Promote Osteointegration.

Osteoimmunomodulation has been considered to play a key role in osteointegration of orthopedic biomaterials. However, regulation of the macrophage phenotype in vivo with a spatiotemporal controllable way still remains a challenge. In this study, we designed a novel magnetic-responsive mineralized collagen coating to exert remotely controlled magneto-mechanical stimulation on macrophages using an external magnetic field. The magneto-mechanical stimulation exhibited immunomodulatory capability to activate M2 macrophage polarization via triggering the integrin-related cascade pathway and suppressing the phosphorylation of JNK in the MAPK pathway. The optimized inflammatory microenvironment subsequently promoted the osteogenic differentiation of bone marrow-derived mesenchymal stem cells and the osteointegration in vivo. This work, therefore, provides a remote spatiotemporal controllable strategy to promote the osteointegration of orthopedic biomaterials via regulation of the osteoimmune microenvironment.

Mesoporous polydopamine nanoparticles for sustained release of rapamycin and reactive oxygen species scavenging to synergistically accelerate neurogenesis after spinal cord injury.

Spinal cord injury (SCI) is an intractable condition with complex pathological processes and poor prognosis. Reactive oxygen species (ROS) generation induced by the mammalian target of the rapamycin (mTOR) protein is one of the causes of secondary inflammation of SCI. Rapamycin (Rapa) is a pharmacological inhibitor of mTOR, which can inhibit ROS overproduction mediated by abnormal activation of the mTOR protein. Polydopamine, as a nanocarrier with excellent biological safety, has been reported to possess satisfactory ROS scavenging ability. Therefore, we designed a mesoporous polydopamine nanoparticle loaded with Rapa (mPDA@Rapa) for combination therapy, which simultaneously inhibited abnormally activated mTOR-mediated ROS production and eliminated already generated ROS. The synthesized mPDA nanoparticles could realize the effective encapsulation and sustained release of Rapa due to their mesoporous cavities and a hydrophobic benzene ring structure. In vitro experiments proved that mPDA@Rapa nanoparticles had a good ROS scavenging ability towards hydrogen peroxide and hydroxyl radicals. Furthermore, mPDA@Rapa also showed a good therapeutic effect in SCI model rats, which was evidenced by a smaller injury cavity, more coordinated hind limb movements, and a higher degree of neurogenesis and tissue regeneration. Our work provides a combined strategy to inhibit ROS overproduction and eliminate excess ROS, with potential applications not only in SCI, but also in other ROS-induced inflammations.

How do humans recognize and face challenges of microplastic pollution in marine environments? A bibliometric analysis.

Microplastics (MPs) are abundant in marine environments, drawing global attention from scientists and rendering it significant to review the research progress and predict future trends of this field. To achieve that, we collected 1898 publications on marine MPs from Web of Science and performed a bibliometric analysis by CiteSpace and VOSviewer. Additionally, we utilized an unrestricted retrieval of literature from ScienceDirect to supplement our major findings. Trends in publication numbers show the growth in study from the initial stage ( 2012 and before), when microplastic (MP) occurrence, abundance, and distribution were primarily investigated. Throughout the ascent stage (between 2013-2016), when diverse sampling and analytical methods were applied to capture and identify MPs from the ocean, baseline data have been gleaned on physiochemical properties of MPs. The research focus then shifted to the bioaccumulation and ecotoxicological effects of MPs on marine biota, further highlighting their potential deleterious impacts on human health via dietary exposure, and this period was defined as the exploration stage (2017 and onwards). Nevertheless, key challenges including the lack of standard procedures for MP sampling, technical limitations in MP detecting and identification, and controversy about the underlying effects on the marine ecosystems and humans have also been arisen in the last decade. The present study elucidates how we gradually recognize MP pollution in marine environments and what challenges we face, suggesting future avenues for MP research.

Therapeutic Agents for the Treatment of Temporomandibular Joint Disorders: Progress and Perspective.

Temporomandibular joint disorders (TMD) are a common health condition caused by the structural or functional disorders of masticatory muscles and the temporomandibular joint (TMJ). Abnormal mandibular movement in TMD patients may cause pain, chronic inflammation, and other discomfort, which could be relieved by a variety of drugs through various delivery systems. In this study, we summarized commonly used therapeutic agents in the management of TMD as well as novel bioactive molecules in preclinical stage and clinical trials. The emerging therapy strategies such as novel intra-TMJ delivery systems and implants based on tissue engineering are also discussed. This comprehensive review will strengthen our understanding of pharmacological approaches for TMD therapy.

Likelihood of needing facial bone augmentation in the anterior maxilla of Chinese Asians: A cone beam computed tomography virtual implant study.

BACKGROUND: The perfect position and axis of an implant is often limited by the morphology of the alveolar ridge, especially in Asians. PURPOSE: The aim of this study was to investigate the frequency at which ideal implant placement position in the anterior maxilla of Chinese Asians would require facial bone augmentation given by an initial facial bone profile. MATERIALS AND METHODS: Implants were virtually placed in 303 sites without tooth loss in the anterior maxilla. The incidence and characters of implant facial plate fenestration were analyzed. If there was no facial fenestration, the thinnest buccal plate (TB) amount adjacent to the implant was measured and recorded. RESULTS: A total of 79 (26.07%) sites showed fenestrations. The mean length, width, and area of exposure in the fenestration sites were 2.94 ± 1.67 mm, 1.52 ± 0.97 mm, and 2.84 ± 3.13 mm2 , respectively. In the nonfenestrated sites, the TB of facial bone around the virtual implants was 1.10 ± 0.78 mm. Among the 224 nonfenestrated sites, 126 sites had a TB of less than 1 mm. CONCLUSIONS: It can be concluded that the initial facial bone profile should be considered when designing restorations and choosing an optimal implant position in the anterior maxilla.

Time course of expression of bcl-2 and bax in rabbit condylar chondrocytes following forward mandibular positioning.

OBJECTIVE: To clarify the expression of Bcl-2 and Bax following forward mandibular positioning (FMP) in the condylar chondrocytes of rabbits. MATERIALS AND METHODS: Sixty rabbits at 8 weeks of age were randomly allocated to the experimental group (n = 36) and control group (n = 24). Rabbits in the experimental group were induced to FMP by a functional appliance. Six rabbits from the experimental group and four from the control group were sacrificed after 3 days and 1, 2, 4, 8, and 12 weeks, respectively. All the right temporomandibular joints (TMJs) were collected and the expression of Bcl-2 and Bax was evaluated by immunohistochemical staining. RESULTS: The results showed the expression pattern of Bcl-2 and Bax during 12 weeks after FMP. The expression of Bcl-2 reached the highest level at 1 week, whereas Bax reached its maximal expression after 4 weeks. Subsequently, the expression of Bcl-2 and Bax gradually decreased. The ratio of Bcl-2/Bax began to decrease 3 days after FMP and continued to decline until 12 weeks. CONCLUSIONS: FMP with functional appliances could change the expression of Bcl-2 and Bax, which is related to apoptosis in condylar chondrocytes.

Differential expression of apoptosis-associated proteins in chondrocytes of the mandibular condyles of rabbits with anterior disk displacement.

Apoptosis is a potential contributor to anterior disk displacement (ADD) in temporomandibular joint disorders (TMD). The aim of this study was to investigate the expression of key protein regulators involved in apoptosis in the chondrocytes of mandibular condyles with induced ADD in experimental animals. ADD was surgically induced in the left temporomandibular joint (TMJ) of 15 rabbits without opening their bursas. A sham operation was performed on the right TMJ without displacing the disk forward. At the end of one, two, and four weeks after surgery, mandibular condyle samples were collected for protein extraction. The production of apoptosis-associated proteins Fas, capase-8, Bcl-2, and Bax was determined using Western blotting. The results indicate that the production of Fas and caspase-8 increased continuously after ADD. Meanwhile, the level of Bcl-2 decreased, and the production of Bax gradually increased following ADD. These results indicate that alterations in protein production of Fas, caspase-8, and the ratio of Bcl-2 to Bax are consistent with an increase of apoptotic activity in the chondrocytes, which may eventually lead to TMD.

Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-κB pathway.

Severe malocclusion can contribute to several serious dental and physical conditions, such as digestive difficulties, periodontal disease, and severe tooth decay. Orthodontic treatment is mainly used to treat malocclusion. Forces in orthodontic tooth results in bone resorption on the pressure side and bone deposition on the tension side. Osteoblasts have been considered as the key component in bone regeneration on the tension side. However, the underlying mechanisms remain unclear. In this study, we focus on how mechanical stretch regulates the osteogenesis during orthodontic treatment. Human jaw bone marrow mesenchymal stem cells (hJBMMSCs) were isolated from healthy adult donors and cultured in regular medium (control) or osteogenic medium (OS). Under OS culture, hJBMMSCs presented osteogenic differentiation potentials, as evidenced by increased mineralization, enhanced calcium deposition, and upregulated expression of osteogenesis markers (ALP, osterix, and Runx). What's more, the OS-induced osteogenesis of hJBMMSCs is associated with the dephosphorylation of IKK, activation of IKBα, and phosphorylation/nucleic accumulation of P65, which all indicated the inhibition of NF-κB activity. Overexpressing P65 in hJBMMSCs, which could constantly activate NF-κB, prevented the osteogenic differentiation in the OS. After that, we applied the Flexcell tension system, which could cause mechanical stretch on cultured hJBMMSCs to mimic the tension forces during tooth movement. Mechanical stretch resulted in 3.5-fold increase of ALP activity and 2.4-fold increase of calcium deposition after 7 days and 21 days treatment, respectively. The expression levels of ALP, Run×2, and Osterix were also significantly upregulated. In the meantime, applying mechanical stretch on OS-cultured hJBMMSCs also dramatically promoted the OS-induced osteogenesis. Both OS and mechanical stretch downregulated NF-κB activity. By overexpressing P65 in hJBMMSCs, neither OS nor mechanical stretch could induce their osteogenesis. These results indicated that, like OS induction, mechanical stretch-facilitated osteogenesis of hJBMMSCs by inhibiting NF-κB in the noninflammatory environments.

Mandibular lateral shift induces the increased expression of TGF-ß, VEGF, and Col-II in the condyle of rat temporomandibular joints.

OBJECTIVE: The present study evaluates histologic changes to and expression of angiogenic factors in rats with mandibular functional shift (MFS). STUDY DESIGN: After 1, 2, and 4 weeks of MFS, rats in the experimental and control groups were killed. Histologic micrographs of the ipsilateral condyle cartilage were obtained. The localization and expression of vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-ß), and type-II collagen (Col-II) in temporomandibular joints (TMJs) were evaluated through immunohistochemical staining. RESULTS: The results showed that structural changes in the condyle cartilage could be observed 2 weeks after MFS. TGF-ß expression reached its peak 2 weeks post-MFS, whereas VEGF and Col-II reached their peaks 4 weeks posttreatment. CONCLUSIONS: Compressive forces applied to the TMJ could enhance the expressions of VEGF, TGF-ß, and Col-II, and activate angiogenesis. The proteins appear to play important roles in the remodeling of the TMJ.