Preparation of a Dual-Functional Sulfated Galactofucan Polysaccharide/Poly(vinyl alcohol) Hydrogel to Promote Macrophage Recruitment and Angiogenic Potential in Diabetic Wound Healing.

A diabetic foot ulcer is a common high-risk complication in diabetic patients, but there is still no universal dressing for clinical treatment. In this study, a novel dual-functional sulfated galactofucan polysaccharide/poly(vinyl alcohol) hydrogel (DPH20) is developed during freeze-thaw cycles. Experimental results indicated that DPH20 had a high specific surface area, a dense porous structure, and a good swelling property, which could effectively adsorb the exudates and keep the wound moist. Furthermore, DPH20 exhibited remarkably recruited macrophage capability and accelerated the inflammation stage by improving the expression of the mRNA of CCL2, CCR2, and CCL22 in macrophages. DPH20 could promote cell migration and growth factor release to accelerate tube formation under hyperglycemic conditions in cell models of L929s and HUEVCs, respectively. Significantly, DPH20 accelerates the reconstruction of the full-thickness skin wound by accelerating the recruitment of macrophages, promoting angiogenesis, and releasing the growth factor in the diabetic mouse model. Collectively, DPH20 is a promising multifunctional dressing to reshape the damaged tissue environment and accelerate wound healing. This study provides an efficient strategy to repair and regenerate diabetic skin ulcers.

Sema3A secreted by sensory nerve induces bone formation under mechanical loads.

Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A (Sema3A), expressed by sensory nerves, in mechanical loads-induced bone formation and nerve withdrawal using orthodontic tooth movement (OTM) model. Firstly, bone formation was activated after the 3rd day of OTM, coinciding with a decrease in sensory nerves and an increase in pain threshold. Sema3A, rather than nerve growth factor (NGF), highly expressed in both trigeminal ganglion and the axons of periodontal ligament following the 3rd day of OTM. Moreover, in vitro mechanical loads upregulated Sema3A in neurons instead of in human periodontal ligament cells (hPDLCs) within 24 hours. Furthermore, exogenous Sema3A restored the suppressed alveolar bone formation and the osteogenic differentiation of hPDLCs induced by mechanical overload. Mechanistically, Sema3A prevented overstretching of F-actin induced by mechanical overload through ROCK2 pathway, maintaining mitochondrial dynamics as mitochondrial fusion. Therefore, Sema3A exhibits dual therapeutic effects in mechanical loads-induced bone formation, both as a pain-sensitive analgesic and a positive regulator for bone formation.

Diagnostic validity of different gonial angle segmentation for the assessment of mandibular growth direction: A retrospective study.

BACKGROUND: The development of the mandible largely determines the sagittal and vertical lateral appearance. The gonial angle (Articulare-Gonion-Menton, Ar-Go'-Me), as a composite indicator, represents the growth direction of the mandible. We proposed a method based on the Frankfort horizontal (FH) plane and its vertical plane (VFH) to divide the gonial angle into sagittal and vertical components (Articulare-Gonion-VFH / Menton-Gonion-FH, Ar-Go'-VFH / Me-Go'-FH) and to compare the accuracy of diagnosing the development of the mandible and maxillofacial structures with other methods. METHODS: Lateral cephalometric films from 736 volunteers aged 6-30 years were collected and analyzed for cephalometric measurements. Four groups of segmentation-based angle, including the FH-based segmentation (Ar-Go'-VFH / Me-Go'-FH), the SN-based segmentation (Articulare-Gonion- Sellion-Nasion plane' vertical plane/ Menton-Gonion- Sellion-Nasion plane, Ar-Go'-VSN / Me-Go'-SN), the Go'-S based segmentation(Articulare-Gonion-Sellion / Menton-Gonion-Sellion, Ar-Go'-S / Me-Go'-S), and the Go'-N based segmentation (Articulare-Gonion-Nasion / Menton-Gonion-Nasion, Ar-Go'-N / Me-Go'-N), as well as commonly used sagittal and vertical indices were measured. Pearson correlation analysis was used to show the representativeness of different segmentation methods on the mandibular growth direction. RESULTS: As the gonial angle decreased with age, all the segmentation-based metrics decreased. The plane-based segmentation metrics, including Ar-Go'-VFH / Me-Go'-FH, Ar-Go'-VSN / Me-Go'-SN, were superior to the point-based segmentation metrics (Ar-Go'-S / Me-Go'-S, and Ar-Go'-N / Me-Go '-N) in evaluating vertical and sagittal development of the mandible. The sagittal indicators displayed alteration of ramus and condyle, while these vertical indicators responded to the alteration of the mandibular corpus and gonial angle. CONCLUSIONS: The gonial angle should be clinically segmented with planes (including SN plane and FH plane) rather than points (including Go'-S and Go'-N) to assess mandibular development. The FH plane-based segmentation method facilitated chair-side diagnosis of the mandibular growth direction.