Glucosamine-Modified Reduction-Responsive Polymeric Micelles for Liver Cancer Therapy.

In this work, glucose transporter-1 (GLUT-1) and glutathione (GSH) over-expression in liver cancer was utilized to design a reduction-responsive and active targeting drug delivery system AG-PEG-SS-PCL (APSP) for the delivery of sorafenib (SF). The SF-APSP micelles were prepared using the thin film hydration method and characterized by various techniques. In vitro release experiments showed that the cumulative release of SF-APSP micelles in the simulated tumor microenvironment (pH 7.4 with GSH) reached 94.76 ± 1.78% at 48 h, while it was only 20.32 ± 1.67% in the normal physiological environment (pH 7.4 without GSH). The in vitro study revealed that glucosamine (AG) enhanced the antitumor effects of SF, and SF-APSP micelles inhibited proliferation by targeting HepG2 cells and suppressing cyclin D1 expression. The in vivo antitumor efficacy study further confirmed that the SF-APSP micelles had excellent antitumor effects and better tolerance against nude mouse with HepG2 cells than other treatment groups. All in all, these results indicated that SF-APSP micelles could be a promising drug delivery system for anti-hepatoma treatment.

Research on preparation and antitumor activity of redox-responsive polymer micelles co-loaded with sorafenib and curcumin.

A novel redox-responsive mPEG-SS-PLA (PSP) polymeric micelle was synthesized and prepared for the delivery of sorafenib (SAF) and curcumin (CUR). And a series of validations were conducted to confirm the structure of the synthesized polymer carriers. Using the Chou-Talalay approach, the combination indexes (CI) of SAF and CUR were determined, and explore the inhibitory effects of the two drugs on HepG2R cells at different ratios. SAF/CUR-PSP polymeric micelles were prepared by thin film hydration method, and the physicochemical properties of nanomicelles were evaluated. The biocompatibility, cell uptake, cell migration, and cytotoxicity assays were assessed in HepG2R cells. The expression of the phosphoinositol-3 kinase (PI3K)/serine/threonine kinase (Akt) signaling pathway was detected by Western blot assay. Additionally, the tumor suppressive effect of SAF/CUR-PSP micelles was clearly superior to free drug monotherapy or their physical combination in HepG2 cell-induced tumor xenografts. The current study revealed that mPEG-SS-PLA polymer micelles loaded with SAF and CUR showed the enhanced therapeutic effects against hepatocellular carcinoma in vitro and in vivo models. It has promising applications for cancer therapy.

Trueness of One Stationary and Two Mobile Systems for Three-Dimensional Facial Scanning.

PURPOSE: To evaluate the trueness of one stationary and two mobile systems for 3D facial scanning. MATERIALS AND METHODS: Twenty participants were included in this study. After marking facial soft tissue landmarks, their faces were scanned using three facial scanning systems: the Bellus3D Dental Pro app on an iPad Pro 2020 (Apple; IP); the ARC-7 Face Scanning System (Bellus3D; BA); and the EinScan Pro 2X Plus (Shining 3D Tech; EP) following the manufacturers' operating instructions. Three-dimensional images were reconstructed with corresponding software and saved in object (OBJ) file format. The interlandmark distances were measured and compared to direct caliper measurements, and absolute error (AE) was chosen as the measurement to determine the trueness of the three scanners. The normal distribution and variance of homogeneity were measured, and then the data were analyzed using one-way ANOVA or Kruskal-Wallis H test. The significance level was set at P = .05. RESULTS: For the measurement of interlandmark distances, no significant differences were found among the four measuring techniques, and the mean AEs of the IP, BA, and EP systems were 1.17 ± 0.80 mm, 0.76 ± 0.61 mm, and 0.69 ± 0.65 mm. CONCLUSION: The three facial scanning systems tested provided a reliable 3D facial reconstruction. The portable IP system could meet the clinical requirements for facial scanning, but it is suggested to select the EP and BA systems when a higher trueness is required.