RESUMEN
Mechanical force across sutures is able to promote suture osteogenesis. Orthodontic clinics often use this biological characteristic of sutures to treat congenital cranio-maxillofacial malformations. However, the underlying mechanisms still remain poorly understood. Craniofacial sutures provide a special growth source and support primary sites of osteogenesis. Here, we isolated rat sagittal suture cells (rSAGs), which had mesenchymal stem cell characteristics and differentiating abilities. Cells were then subjected to mechanical tension (5% elongation, 0.5 Hz; sinusoidal waveforms) showing that mechanical tension could enhance osteogenic differentiation but hardly affect proliferation of rSAGs. Besides, mechanical tension could increase Rho-associated kinase (ROCK) expression and enhance transcriptional coactivator with PDZ-binding motif (TAZ) nuclear translocation. Inhibiting ROCK expression could suppress tension-induced osteogenesis and block tension-induced upregulation of nuclear TAZ. In addition, our results indicated that TAZ had direct combination sites with runt-related transcription factor 2 (Runx2) in rSAGs, and knock-downed TAZ simultaneously decreased the expression of Runx2 no matter with or without mechanical tension. In summary, our findings demonstrated that the multipotency of rSAGs in vitro could give rise to early osteogenic differentiation under mechanical tension, which was mediated by ROCK-TAZ signal axis.
Asunto(s)
Subunidad alfa 1 del Factor de Unión al Sitio Principal/genética , Suturas Craneales/metabolismo , Péptidos y Proteínas de Señalización Intracelular/genética , Osteogénesis/genética , Transactivadores/genética , Quinasas Asociadas a rho/genética , Animales , Diferenciación Celular/genética , Suturas Craneales/crecimiento & desarrollo , Suturas Craneales/patología , Fenómenos Mecánicos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Ratas , Transducción de Señal/genética , Proteínas Coactivadoras Transcripcionales con Motivo de Unión a PDZRESUMEN
PURPOSE: The aim of this study is to locate pterygopalatine fossa (PPF) and the opening of its communicating canals by accessing the relationship between PFF and the endoscopic landmarks such as the tubercular recess (TR) and middle lowest point of sellar floor (SF) as well as analyze the relation between PPF and important structures such as internal carotid artery (ICA) and optic canal (OC). MATERIALS AND METHODS: Computer topographic angiography (CTA) images of 118 PPF regions were reviewed. The measurement was on coronal, sagittal, and axial planes after multiplanar reconstruction (MPR). The location of PPF and its relationship to the sphenoid sinus, ICA, and OC were studied. The communicating canals of PPF, which were related to the transsphenoid approach, were three-dimensionally measured by the stationary structures, such as the middle lowest point of SF, the sagittal midline, and the top and bottom wall of sphenoid sinus. RESULT: The posterior part of PPF was located by the middle lowest point of SF. The anterior opening of sphenopalatine foramen (SPF), pterygoid canal (PC), palatovaginal canal (PVC), and foramina rotundum (FR) have relative stationary position, which can be located by the landmarks of sellar region during the endoscopic surgery. CONCLUSIONS: Pterygopalatine fossa is related to numerous neurovascular structures. Accurate understanding of the radiologic anatomy of PPF is beneficial for the PPF disease diagnosis, the selection of treatment plan and the prognosis evaluation.
Asunto(s)
Fosa Pterigopalatina/diagnóstico por imagen , Silla Turca/diagnóstico por imagen , Adolescente , Adulto , Anciano , Puntos Anatómicos de Referencia/diagnóstico por imagen , Angiografía/métodos , Arteria Carótida Interna/diagnóstico por imagen , Endoscopía/métodos , Femenino , Humanos , Procesamiento de Imagen Asistido por Computador/métodos , Imagenología Tridimensional/métodos , Masculino , Persona de Mediana Edad , Órbita/diagnóstico por imagen , Paladar Duro/diagnóstico por imagen , Hueso Esfenoides/diagnóstico por imagen , Seno Esfenoidal/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Adulto JovenRESUMEN
Microarc oxidation (MAO) has become a promising technique for the surface modification of implants. Therefore, the aims of this study were to further quantitatively and qualitatively evaluate the osteointegration abilities of MAO-treated and smooth surface (SF) implants in vivo and to investigate the areas in which the superiority of MAO-treated implants are displayed. In a rabbit model, a comprehensive histomorphological, osteogenic, mineralizational, and integrative assessment was performed using light microscopy, fluorescence microscopy, confocal laser scanning microscopy, and radiographic analyses. Compared with the SF groups, the MAO-treated groups exhibited more active contact osteogenesis, as well as distant osteogenesis, under fluorescence examination, the mineral apposition rate was found to be greater for all of the MAO-treated implants, and the osteointegration index (OI) value was greater in the MAO-treated groups at different times. In conclusion, the calcium-rich amorphous layer created by MAO provided a better environment for osteointegration, with more active contact osteogenesis, a more rapid mineral apposition rate and greater OI values.