Controllable Preparation of Ag-Ce/ZnO Nanorods Photocatalyst and Its Growth on Modified Polyester Surface in Water Bath.

In this paper, a simple and effective method to prepare Ag-Ce/ZnO nanorods photocatalyst and grow them controllably on modified polyester fabrics was presented to fabricate multifunctional textiles. Analytical grade zinc acetate dihydrate and sodium hydroxide were used as the main raw materials to prepare Ag-Ce/ZnO nanorods. Morphological, structural and chemical characterization of the Ag-Ce/ZnO nanorods was performed by XRD, UV-vis and other spectroscopies. The results showed that the Ag-Ce/ZnO nanorods had a hexagonal wurtzite structure. After 60 minutes of irradiation under ultraviolet light, the Ag-Ce/ZnO nanorods showed a percentage photodegradation of 93.14% for a methylene blue (MB) solution. Modified polyester fabrics covered with the Ag-Ce/ZnO nanorods were then prepared in a water bath. By a series of tests, it was observed that the Ag-Ce/ZnO nanorods on the modified polyester surface were neatly arranged and had good photocatalytic properties. Moreover, the UPF of the modified polyester fabric after finishing increased from 30.4 to 877.2. The multifunctional properties of the finished fabric exhibited good durability.

[Quantification and phenotypic analysis of hCMV specific CTL in peripheral blood from HLA-A2+ donors].

AIM: To optimize tetramer staining condition using HLA-A*0201 tetramer (A2-NLV tetramer) loaded with NLV peptide (pp65(495-503)) derived from structural protein pp65 of human cytomegalovirus and to investigate its application in phenotyping of specific cytotoxic T lymphocytes (CTL). METHODS: Peripheral blood from HLA-A2(+) donors was first stained with A2-NLV tetramer/PE under different conditions and then labeled with anti-CD3-FITC and anti-CD8-APC. The stained samples were analyzed with flow cytometry to find out the optimized staining condition. Meanwhile, the phenotype and activation antigen expression were determined. RESULTS: Tetramer staining with whole blood was superior to peripheral blood mononuclear cells. The optimized condition for tetramer staining was incubating 100 muL of whole blood with 0.3 mug of A2-NLV tetramer for 1 h at 4 degrees Celsius. Under this condition the specific staining was strong while unspecific staining of CD8(-) T cells was quite weak. Phenotypic analysis under this condition showed that the ratio of CD28 positive A2-NLV tetramer specific CTL was lower than that of nonspecific CTL, whereas the ratio of CD57 positive specific CTL was higher than that of nonspecific CTL. CD25 molecules were only expressed on the activated specific CTL. CONCLUSION: The optimized tetramer staining condition can increase the specificity of tetramer staining and decrease unspecific binding, therefore it is applicable for phenotyping and functional analysis of antigen-specific CTL.