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Infect Immun ; 72(3): 1657-65, 2004 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-14977973

RESUMEN

The lipopeptide FSL-1 [S-(2,3-bispalmitoyloxypropyl)-Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe, Pam(2)CGDPKHPKSF] synthesized on the basis of the N-terminal structure of a Mycoplasma salivarium lipoprotein capable of activating normal human gingival fibroblasts to induce the cell surface expression of ICAM-1 revealed an activity to induce production of monocyte chemoattractant protein 1, interleukin-6 (IL-6), and IL-8. FSL-1 also activated macrophages to produce tumor necrosis factor alpha as the Mycoplasma fermentans-derived lipopeptide MALP-2 (Pam(2)CGNNDESNISFKEK), a potent macrophage-activating lipopeptide, did. The level of the activity of FSL-1 was higher than that of MALP-2. This result suggests that the difference in the amino acid sequence of the peptide portion affects the activity because the framework structure other than the amino acid sequence of the former is the same as that of the latter. To determine minimal structural requirements for the activity of FSL-1, the diacylglyceryl Cys and the peptide portions were examined for this activity. Both portions did not reveal the activity. A single amino acid substitution from Phe to Arg and a fatty acid substitution from palmitic acid to stearic acid drastically reduced the activity. Similar results were obtained in measuring the NF-kappaB reporter activity of FSL-1 to human embryonic kidney 293 cells transfected with Toll-like receptor 2 and 6, together with a NF-kappaB-dependent luciferase reporter plasmid. These results suggest that both the diacylglyceryl and the peptide portions of FSL-1 are indispensable for the expression of biological activities and for the recognition by Toll-like receptors 2 and 6 and that the recognition of FSL-1 by Toll-like receptors 2 and 6 appears to be hydrophobic.


Asunto(s)
Proteínas Bacterianas/química , Proteínas Bacterianas/inmunología , Diglicéridos/química , Diglicéridos/inmunología , Glicoproteínas de Membrana/metabolismo , Mycoplasma salivarium/inmunología , Oligopéptidos/química , Oligopéptidos/inmunología , Receptores de Superficie Celular/metabolismo , Acilación , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Proteínas Bacterianas/genética , Proteínas Bacterianas/toxicidad , Línea Celular , Diglicéridos/genética , Diglicéridos/toxicidad , Fibroblastos/inmunología , Encía/inmunología , Humanos , Lipopéptidos , Glicoproteínas de Membrana/genética , Datos de Secuencia Molecular , Estructura Molecular , Monocitos/inmunología , Mycoplasma fermentans/genética , Mycoplasma fermentans/inmunología , Mycoplasma fermentans/patogenicidad , Mycoplasma salivarium/genética , Mycoplasma salivarium/patogenicidad , Oligopéptidos/genética , Oligopéptidos/toxicidad , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores de Superficie Celular/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Relación Estructura-Actividad , Receptor Toll-Like 2 , Receptor Toll-Like 6 , Receptores Toll-Like
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