RESUMEN
Cellulose in different forms has extensively been applied in biomedical treatments, including scaffolding, tissue engineering and tissue formation. To evaluate the therapeutic effects of a liquid bandage (LB) prepared with cellulose powders from Styela clava tunics (SCT) and Broussonetia kazinoki bark (BSLB) for healing cutaneous wounds, the remedial effects of a low concentration (LoBSLB) and a high concentration (HiBSLB) of BSLB on skin regeneration and toxicity in Sprague Dawley rats. Results indicated that the total area of skin involved in the surgical wound was lower in the BSLBtreated group compared with the Vehicletreated group at days 412, although some variations were observed in the HiBSLBtreated group. In addition, the BSLBtreated group showed significantly enhanced width of the reepithelialization region and epidermal thickness when compared with the Vehicletreated group. Furthermore, significant stimulation in the expression level of collagen1 and the signaling pathway of VEGF after topical application of BSLB was indicated. No liver or kidney toxicities were detected for either doses of BSLB. Overall, the results of the present study suggest that BSLB accelerates the process of wound healing in surgical skin wounds of Sprague Dawley rats through stimulation of reepithelialization and connective tissue formation, without any accompanying significant toxicity.
Asunto(s)
Broussonetia/química , Celulosa/farmacología , Polvos/farmacología , Piel/efectos de los fármacos , Herida Quirúrgica/tratamiento farmacológico , Urocordados/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Vendajes , Colágeno Tipo I/metabolismo , Epidermis/efectos de los fármacos , Epidermis/metabolismo , Masculino , Ratas , Ratas Sprague-Dawley , Piel/metabolismo , Herida Quirúrgica/metabolismo , Ingeniería de Tejidos/métodosRESUMEN
PURPOSE: Aims of this study is to evaluate the therapeutic effects and toxicity of Se-loaded cellulose film originated from Styela clava tunic (SeSCTF) on cutaneous wounds during diabetic conditions. MATERIALS AND METHODS: Alterations in skin regeneration, angiogenesis and toxicity were examined using streptozotocine (STZ)-induced diabetic Sprague Dawley® (SD) rats with surgical skin wounds after application of SeSCTF for 12 days. RESULTS: SCTF showed high tensile strength (1.64 MPa), low elongation (28.59%), low water vapor transmission rate (WVTR) and outstanding porous structure. Although SeSCTF application did not induce any significant alterations in glucose concentration or toxicity, wound morphology was rapidly recovered in the SeSCTF treated group relative to the gauze (GZ) and SCTF treated group. Moreover, recovery of re-epithelization, wound contraction and number of blood vessel was observed in SeSCTF treated groups when compared with all other groups. Furthermore, the SeSCTF treated group showed complete recovery of key protein expressions of the downstream signaling pathway of vascular endothelial growth factor (VEGF), angiopoietin-2/1 (Ang-2/1), the signaling pathway of insulin receptors and anti-oxidative status. CONCLUSIONS: Overall, the results of this study suggest that SeSCTF accelerates the healing process of cutaneous wounds in STZ-induced diabetic SD rats through stimulation of angiogenesis and the glucose receptor signaling pathway.