[Efficacy of photodynamic therapy for peri-implantitis: A meta-analysis].

PURPOSE: This meta-analysis was undertaken to evaluate the efficacy of photodynamic therapy (PDT) in combination with non-surgical therapy compared to non-surgical therapy alone for patients with peri-implantitis. METHODS: Bibliographic databases including Cochrane Library, PubMed, EMbase, Web of Science, CNKI, VIP, Wanfang Data and CBM were searched from inception to April 1st 2018 , to collect randomized controlled trials (RCTs) of photodynamic therapy in combination with non-surgical therapy and non-surgical therapy alone for peri-implantitis. Two reviewers independently screened the literature according to the inclusion and exclusion criteria, extracted the data, and assessed the quality. Then after cross-checking, meta analyses was performed by using RevMan 5.3 software. Meta analysis of probing depth (PD), bleeding on probing(BOP) and clinical attachment level(CAL) was conducted using weighted mean differences (WMD) for continuous data with 95% confidence intervals(CI). RESULTS: Six studies met the inclusion criteria. Meta analysis results showed that compared to non-surgical therapy alone, PDT in combination with non-surgical therapy showed significant differences in PD(WMD=-0.81,95%CI -1.52 to -0.11, P=0.02), BOP(WMD=-5.15, 95%CI -6.29 to -4.01, P<0.00001), but no significant differences in CAL(WMD=-1.13, 95%CI -3.51 to 1.25, P=0.35). CONCLUSIONS: In the treatment of peri-implantitis, compared to non-surgical therapy alone, non-surgical therapy with PDT was more effective in reduction of probing depth and probing bleeding, but it did not significantly improve clinical attachment level. For the quantity and quality limitation of the included studies, the conclusion has to be further proved by performing more well-designed and high quality RCTs.

Global transcriptomic analysis of human neuroblastoma cells in response to enterovirus type 71 infection.

Human enterovirus type 71 (EV71) is the major pathogen of hand-foot-and-mouth disease (HFMD) and has been associated with severe neurological disease and even death in infants and young children. The pathogenesis of EV71 infection in the human central nervous system remains unclear. In this study, human whole genome microarray was employed to perform transcriptome profiling in SH-SY5Y human neuroblastoma cells infected with EV71. The results indicated that EV71 infection lead to altered expression of 161 human mRNAs, including 74 up-regulated genes and 87 down-regulated genes. Bioinformatics analysis indicated the possible roles of the differentially regulated mRNAs in selected pathways, including cell cycle/proliferation, apoptosis, and cytokine/chemokine responses. Finally, the microarray results were validated using real-time RT-PCR with high identity. Overall, our results provided fundamental information regarding the host response to EV71 infection in human neuroblastoma cells, and this finding will help explain the pathogenesis of EV71 infection and virus-host interaction.