RESUMEN
The residues of phenothiazines and benzodiazepines in foods of animal origin are dangerous to consumers. For inspection of their abuses, this study for the first time reported on the use of a chemiluminescence array sensor for the simultaneous determination of four phenothiazines and five benzodiazepines in pig urine. Two molecularly imprinted polymers were coated in different wells of a conventional 96-well microtiter plate as the recognition reagents. After sample loading, the absorbed analytes were initiated directly by using an imidazole enhanced bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide system to emit light. The assay process consisted of only one sample-loading step prior to data acquisition, so one test was finished within 10 min. The limits of detection for the nine drugs in the pig urine were in a range of 0.1 to 0.6 pg/mL, and the recoveries from the fortified blank urine samples were in a range of 80.3 to 95%. Furthermore, the sensor could be reused six times. Therefore, this sensor could be used as a simple, rapid, sensitive and reusable tool for routine screening for residues of phenothiazines and benzodiazepines in pig urine.
Asunto(s)
Benzodiazepinas/orina , Mediciones Luminiscentes/métodos , Fenotiazinas/orina , Polímeros/química , Animales , Diseño de Equipo , Peróxido de Hidrógeno/química , Límite de Detección , Mediciones Luminiscentes/instrumentación , Microscopía Electrónica de Rastreo , Impresión Molecular , Nitrazepam/química , Oxalatos/química , Prometazina/química , Sensibilidad y Especificidad , Porcinos , Factores de TiempoRESUMEN
The 3D structures of two dummy templates and four phenothiazine drugs were studied by using computational simulation method. Then the two dummy templates were used to synthesize two molecularly imprinted polymers respectively. Results showed that the recognition abilities of the two polymers were consistent with the theoretical calculation. Then a solid phase extraction column was developed for extraction of the four phenothiazines in meat (pork, chicken) followed by determination with high performance liquid chromatography. The column showed high adsorption capacities (850-962ng analyte per milligram of polymer) and high recoveries (93-98%) to the four drugs, and could be recycled for sixty times. The limits of detection were in the range of 1.0-10ng/g, and the recoveries from the fortified blank samples were in the range of 70.3-96.1%. This is the first study reporting the use of molecularly imprinted polymer-based method for determination of phenothiazines residues in foods.