RESUMEN
Terbium doped graphitic carbon nitride (g-C3N4:Tb) gives rise to two exceptional emissions at λex/λem = 290/490 nm and 290/546 nm, with extremely narrow peak widths of FWHM < 12 nm as well as a large Stokes shift of >200 nm. The modification of g-C3N4:Tb with HOOC-PEG-COOH provides a ratiometric fluorescent probe which ensures highly sensitive detection of alkaline phosphatase (ALP) activity based on the inner filter effect (IFE).
Asunto(s)
Fosfatasa Alcalina/sangre , Colorantes Fluorescentes/química , Grafito/química , Compuestos de Nitrógeno/química , Terbio/química , Animales , Bovinos , Humanos , Límite de Detección , Nanoestructuras/química , Polietilenglicoles/química , Conejos , Espectrometría de FluorescenciaRESUMEN
In this study, CuS@PDA nanoparticles were synthesized and used to create a novel tumor-targeting nanocomposite platform composed of copper sulfide@polydopamine-folic acid/doxorubicin (CuS@PDA-FA/DOX) for performing both photothermal and chemotherapeutic cancer treatment. The nanocomposite platform has ultrahigh loading levels (4.2 ± 0.2 mg mg-1) and a greater photothermal conversion efficiency (η = 42.7%) than CuS/PDA alone. The uptake of CuS@PDA-FA/DOX nanocomposites is much higher in MCF-7 cells than in A549 cells because MCF-7 cells have much higher folic acid receptors than A549. Under near infrared (NIR) irradiation, the CuS@PDA-FA/DOX system using a synergistic combination of photothermal therapy and chemotherapy yields a better therapeutic effect than either photothermal therapy or chemotherapy alone. The treatment is very effective with the cell viability is only 5.6 ± 1.4%.