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INTRODUCTION: Lanqin Oral Liquid (LQL) is a traditional Chinese medicine preparation (TCMP) containing five herbal medicines and has been commonly used for the treatment of pharyngitis and hand-foot-and-mouth disease in clinic. The material basis of LQL has been reported in our previous study, but the contents of the major components and the features of saccharide in LQL are still unclear. OBJECTIVES: This study aimed to establish accurate and rapid methods for the quantification of the major components and profiling of saccharide in LQL. The quantitative results combined with similarity evaluation were applied to improve the quality control of LQL. METHODOLOGY: An ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-QQQ-MS) method was utilised to determine 44 major components. Cosine similarity was used to evaluate the similarities among 20 batches of LQL based on the quantitative results of 44 major components. The physicochemical properties, structure, composition, and contents of saccharide in LQL were detected by a combination of chemical and instrumental analysis. RESULTS: A total of 44 compounds, including flavonoids, iridoid glycosides, alkaloids, and nucleosides, were accurately determined. The 20 batches of LQL were remarkably similar (> 0.95). In addition, d-glucose, galactose, d-glucuronic acid, arabinose, and d-mannose were detected in saccharide of LQL. The contents of saccharide in LQL were 13.52-21.09 mg/ml. CONCLUSIONS: The established methods can be applied for the comprehensive quality control of LQL, including characterisation of saccharide and quantification of representative components. Our study will provide a robust chemical foundation for disclosing the quality markers of its therapeutic effect.
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Medicamentos Herbarios Chinos , Medicamentos Herbarios Chinos/química , Medicina Tradicional China , Espectrometría de Masas en Tándem/métodos , Flavonoides/análisis , Control de Calidad , Cromatografía Líquida de Alta Presión/métodosRESUMEN
BACKGROUND: The repair of large bone defects is a great challenge for orthopedics. Although the development of three-dimensional (3D) printed titanium alloy (Ti6Al4V) implants with optimized the pore structure have effectively promoted the osseointegration. However, due to the biological inertia of Ti6Al4Vsurface and the neglect of angiogenesis, some patients still suffer from postoperative complications such as dislocation or loosening of the prosthesis. METHODS: The purpose of this study was to construct 3D printed porous Ti6Al4V scaffolds filled with bone marrow mesenchymal stem cells (BMSC) and endothelial progenitor cells (EPC) loaded hydrogel and evaluate the efficacy of this composite implants on osteogenesis and angiogenesis, thus promoting osseointegration. RESULTS: The porosity and pore size of prepared 3D printed porous Ti6Al4V scaffolds were 69.2 ± 0.9 % and 593.4 ± 16.9 µm, respectively, which parameters were beneficial to bone ingrowth and blood vessel formation. The BMSC and EPC filled into the pores of the scaffolds after being encapsulated by hydrogels can maintain high viability. As a cell containing composite implant, BMSC and EPC loaded hydrogel incorporated into 3D printed porous Ti6Al4V scaffolds enhancing osteogenesis and angiogenesis to repair bone defects efficiently. At the transcriptional level, the composite implant up-regulated the expression levels of the osteogenesis-related genes alkaline phosphatase (ALP) and osteocalcin (OCN), and angiogenesis-related genes hypoxia-inducible factor 1 alpha (HIF-1α), and vascular endothelial growth factor (VEGF). CONCLUSIONS: Overall, the strategy of loading porous Ti6Al4V scaffolds to incorporate cells is a promising treatment for improving osseointegration.
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Oseointegración , Osteogénesis , Aleaciones , Humanos , Porosidad , Impresión Tridimensional , Andamios del Tejido , Titanio , Factor A de Crecimiento Endotelial VascularRESUMEN
Gastrodia elata is a well-known medicinal and heterotrophic orchid. Its germination, limited by the impermeability of seed coat lignin and inhibition by abscisic acid (ABA), is triggered by symbiosis with fungi such as Mycena spp. However, the molecular mechanisms of lignin degradation by Mycena and ABA biosynthesis and signaling in G. elata remain unclear. In order to gain insights into these two processes, this study analyzed the transcriptomes of these organisms during their dynamic symbiosis. Among the 25 lignin-modifying enzyme genes in Mycena, two ligninolytic class II peroxidases and two laccases were significantly upregulated, most likely enabling Mycena hyphae to break through the lignin seed coats of G. elata. Genes related to reduced virulence and loss of pathogenicity in Mycena accounted for more than half of annotated genes, presumably contributing to symbiosis. After coculture, upregulated genes outnumbered downregulated genes in G. elata seeds, suggesting slightly increased biological activity, while Mycena hyphae had fewer upregulated than downregulated genes, indicating decreased biological activity. ABA biosynthesis in G. elata was reduced by the downregulated expression of 9-cis-epoxycarotenoid dioxygenase (NCED-2), and ABA signaling was blocked by the downregulated expression of a receptor protein (PYL12-like). This is the first report to describe the role of NCED-2 and PYL12-like in breaking G. elata seed dormancy by reducing the synthesis and blocking the signaling of the germination inhibitor ABA. This study provides a theoretical basis for screening germination fungi to identify effective symbionts and for reducing ABA inhibition of G. elata seed germination.
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Ácido Abscísico/metabolismo , Agaricales/patogenicidad , Proteínas Fúngicas/genética , Gastrodia/microbiología , Lignina/metabolismo , Proteínas de Plantas/genética , Agaricales/genética , Agaricales/metabolismo , Dioxigenasas/genética , Dioxigenasas/metabolismo , Proteínas Fúngicas/metabolismo , Gastrodia/genética , Gastrodia/crecimiento & desarrollo , Gastrodia/metabolismo , Germinación , Lacasa/genética , Lacasa/metabolismo , Lignina/genética , Peroxidasas/genética , Peroxidasas/metabolismo , Proteínas de Plantas/metabolismo , Simbiosis , TranscriptomaRESUMEN
BACKGROUND: Currently, in the field of total joint arthroplasty (TJA), there are no studies that have demonstrated the value of the sequential application of hydrogen peroxide, povidone-iodine, and physiological saline during the surgical procedure in decreasing postoperative infections in total knee arthroplasty (TKA), and in decreasing the incidence of periprosthetic joint infections (PJI) in particular. This study aimed to assess the efficacy of the sequential application of hydrogen peroxide, povidone-iodine, and physiological saline in reducing postoperative infections in TKA. METHODS: The study prospectively included 4743 patients, with Group A (2371, 49.9%) receiving sequential intraoperative application of hydrogen peroxide, povidone-iodine, and physiological saline irrigation of the incision, and Group B (2372, 50.1%) receiving intraoperative application of physiological saline irrigation of the incision only, to collect the patients' baseline data and clinical characteristics, and to statistically assess the incidence of superficial infections and the PJI during the follow up period to evaluate the clinical value of the study. RESULTS: The baseline levels of patients in Groups A and B were comparable. There were 132 (2.8%) lost visits during the study period. The incidence of superficial infections within 30 days after surgery was 0.22% in Group A and 1.17% in Group B, the difference between the two groups was statistically significant (P = 0.007). The incidence of PJI was 0.17% in Group A and 1.26% in Group B, the difference between the two groups was statistically significant (P = 0.0121). CONCLUSION: Sequential application of hydrogen peroxide, povidone-iodine, and physiological saline to irrigate incision in TKA can significantly reduce the incidence of postoperative superficial infections and PJI. The scientific and rational application of this therapy intraoperatively greatly reduces the incidence of PJI and postoperative superficial infections, which is of great benefit to the patient's prognosis.
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Antiinfecciosos Locales , Artroplastia de Reemplazo de Rodilla , Peróxido de Hidrógeno , Povidona Yodada , Infecciones Relacionadas con Prótesis , Solución Salina , Infección de la Herida Quirúrgica , Humanos , Artroplastia de Reemplazo de Rodilla/efectos adversos , Povidona Yodada/administración & dosificación , Povidona Yodada/uso terapéutico , Peróxido de Hidrógeno/administración & dosificación , Masculino , Femenino , Estudios Prospectivos , Antiinfecciosos Locales/administración & dosificación , Anciano , Persona de Mediana Edad , Infección de la Herida Quirúrgica/prevención & control , Infección de la Herida Quirúrgica/epidemiología , Infección de la Herida Quirúrgica/etiología , Infecciones Relacionadas con Prótesis/prevención & control , Infecciones Relacionadas con Prótesis/etiología , Infecciones Relacionadas con Prótesis/epidemiología , Solución Salina/administración & dosificación , Irrigación Terapéutica/métodos , IncidenciaRESUMEN
Lignocellulosic biomass valorization is regarded as a promising approach to alleviate energy crisis and achieve carbon neutrality. Bioactive enzymes have attracted great attention and been commonly applied for biomass valorization owing to their high selectivity and catalytic efficiency under environmentally benign reaction conditions. Same as biocatalysis, photo-/electro-catalysis also happens at mild conditions (i.e., near ambient temperature and pressure). Therefore, the combination of these different catalytic approaches to benefit from their resulting synergy is appealing. In such hybrid systems, harness of renewable energy from the photo-/electro-catalytic compartment can be combined with the unique selectivity of biocatalysts, therefore providing a more sustainable and greener approach to obtain fuels and value-added chemicals from biomass. In this review, we firstly introduce the pros/cons, classifications, and the applications of photo-/electro-enzyme coupled systems. Then we focus on the fundamentals and comprehensive applications of the most representative biomass-active enzymes including lytic polysaccharide monooxygenases (LPMOs), glucose oxidase (GOD)/dehydrogenase (GDH) and lignin peroxidase (LiP), together with other biomass-active enzymes in the photo-/electro- enzyme coupled systems. Finally, we propose current deficiencies and future perspectives of biomass-active enzymes to be applied in the hybrid catalytic systems for global biomass valorization.
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Lignina , Biomasa , Catálisis , BiocatálisisRESUMEN
Current cellulose-based adsorbents suffer from the drawbacks of low adsorption capacity or slow adsorption rate for heavy metal ions. It is imperative to prepare new cellulose-based materials to improve the adsorption ability. In this work, we aim to introduce phosphonate groups to improve the adsorption ability of cellulose and select polyethyleneimine (PEI) for synergistic adsorption. A novel cellulose phosphonate/polyethyleneimine composite (MCCP-PEI) is prepared via the Mannich reaction. The structure and composition of MCCP-PEI are characterized by various advanced microscopy and spectroscopy techniques, and the results show that MCCP-PEI possesses abundant nano-porous structure, strong chelating sites, and excellent hydrophilicity. Besides, the adsorption behavior of MCCP-PEI for heavy metals has been systematically investigated. The results show that the adsorbent can quickly remove toxic Cu(II) and Pb(II) from water within 15 min and 20 min, respectively. The saturated adsorption capacity for Cu(II) and Pb(II) is 250.0 and 534.7 mg·g-1, respectively. X-ray photoelectron spectroscopy analysis combined with Density Functional Theory calculations reveal that the adsorption mechanism is chemical complexation and electrostatic attraction, and the phosphonate group plays a key role in the adsorption process.
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Metales Pesados , Contaminantes Químicos del Agua , Agua , Polietileneimina/química , Plomo , Celulosa , Metales Pesados/química , Contaminantes Químicos del Agua/química , Adsorción , CinéticaRESUMEN
To elucidate the genomic traits of ecological diversification in the Hymenochaetales, we sequenced 15 new genomes, with attention to ectomycorrhizal (EcM) Coltricia species. Together with published data, 32 genomes, including 31 Hymenochaetales and one outgroup, were comparatively analyzed in total. Compared with those of parasitic and saprophytic members, EcM species have significantly reduced number of plant cell wall degrading enzyme genes, and expanded transposable elements, genome sizes, small secreted proteins, and secreted proteases. EcM species still retain some of secreted carbohydrate-active enzymes (CAZymes) and have lost the key secreted CAZymes to degrade lignin and cellulose, while possess a strong capacity to degrade a microbial cell wall containing chitin and peptidoglycan. There were no significant differences in secreted CAZymes between fungi growing on gymnosperms and angiosperms, suggesting that the secreted CAZymes in the Hymenochaetales evolved before differentiation of host trees into gymnosperms and angiosperms. Nevertheless, parasitic and saprophytic species of the Hymenochaetales are very similar in many genome features, which reflect their close phylogenetic relationships both being white rot fungi. Phylogenomic and molecular clock analyses showed that the EcM genus Coltricia formed a clade located at the base of the Hymenochaetaceae and divergence time later than saprophytic species. And Coltricia remains one to two genes of AA2 family. These indicate that the ancestors of Coltricia appear to have originated from saprophytic ancestor with the ability to cause a white rot. This study provides new genomic data for EcM species and insights into the ecological diversification within the Hymenochaetales based on comparative genomics and phylogenomics analyses.
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Basidiomycota , Micorrizas , Filogenia , Genómica , Lignina/metabolismoRESUMEN
Hand, foot and mouth disease is a common acute viral infectious disease that poses a serious threat to the life and health of young children. With the development of an effective inactivated EV71 vaccine, CA16 has become the main pathogen causing HFMD. Effective and safe vaccines against this disease are urgently needed. In our previous study, a bivalent inactivated vaccine was shown to have good immunogenicity and to induce neutralizing antibodies in mice and monkeys. Repeated administration toxicity is a critical safety test in the preclinical evaluation of vaccines. In this study, BALB/c mice were used to evaluate the toxicity of the bivalent vaccine after multiple intradermal administrations. Clinical observation was performed daily, and body weight, food intake, hematological characteristics, serum biochemical parameters, antinuclear antibodies, CD4+/CD8a+ T-cell proportions, bone marrow smear results and pathology results were recorded. The results showed that there was no significant change at the injection site and no adverse reactions related to the vaccine. The bivalent inactivated EV71-CA16 vaccine exhibits good safety in mice, and these results provide a sufficient basis for further clinical trials.
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Enterovirus Humano A , Enfermedad de Boca, Mano y Pie , Vacunas Virales , Animales , Ratones , Enfermedad de Boca, Mano y Pie/prevención & control , Anticuerpos Antivirales , Vacunas de Productos Inactivados , Anticuerpos Neutralizantes , Ratones Endogámicos BALB CRESUMEN
The flapping-motion of the caudal fin allows the fish to swim with high efficiency and mobility, particularly in terms of persistence, propulsion, and acceleration. This has led to theoretical and practical research on the development of robotic caudal fin thrusters that offer similar properties and performance. However, the current caudal fin thrusters are driven by electromagnetic motors, which require a transmission system that makes them difficult to miniaturize, and need protection against water intrusion. To address these issues, this paper proposes a novel piezoelectric caudal fin thruster with a fully open structure that has no chambers in any of its parts. The converse, piezoelectric effect and direct friction drive principle are used to make a rotation unit for the piezoelectric actuator drive and achieve a reciprocating motion that makes the caudal fin flap. The proposed piezoelectric caudal fin thruster has an open and simple structure. It has a weight of 30 g, a length of 89 mm, and a thrust of 0.07 N. It is easy to miniaturize and is lighter, smaller, and more efficient than previously reported caudal fin thrusters that were based on ionic polymer-metal composites and shape memory alloys. Experimental results verified the effectiveness of the proposed design, which can be easily scaled up or down in size depending on the operating situation.
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Aceleración , Vibración , Animales , Fricción , Polímeros , RotaciónRESUMEN
Micro/nanoplastics (MNPs) are widespread environmental pollutants that cause high health risks. However, high heterogeneity in particle sizes and chemical compositions of MNPs make their accurate characterization extremely challenging. Herein, we established a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) strategy for the unambiguous characterization of different types of MNPs with high performance, including polystyrene, polyethylene glycol terephthalate, polyamide, polymethyl methacrylate, acrylonitrile butadiene styrene copolymer, and polycarbonate. The MNP sample preparation and detection conditions were systematically optimized by using response surface methodology, and the MS detection signal-to-noise ratios were improved 1.5 times on average. The ultrahigh mass resolution of FTICR MS is crucial to the unambiguous elucidation of MNP structures. We demonstrate that this MS strategy is highly efficient in the characterization of polymer constitutions of environmental MNPs derived from foam, bottles, cable ties, and compact discs, providing a promising tool for MNP detection and safety evaluation.
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Acrilonitrilo , Contaminantes Ambientales , Butadienos , Contaminantes Ambientales/análisis , Análisis de Fourier , Microplásticos , Nylons , Polietilenglicoles , Polímeros , Polimetil Metacrilato , Poliestirenos/análisis , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodosRESUMEN
Phylogenetic analyses inferred from the nuc rDNA ITS1-5.8S-ITS2 (ITS) data set and the combined 2-locus data set [5.8S + nuc 28S rDNA (nLSU)] of taxa of Trechisporales around the world show that Sistotremastrum family forms a monophyletic lineage within Trechisporales. Bayesian evolutionary and divergence time analyses on two data sets of 5.8S and nLSU sequences indicate an ancient divergence of Sistotremastrum family from Hydnodontaceae during the Triassic period (224.25 Mya). Sistotremastrum family is characterized by resupinate and thin basidiomata, smooth, verruculose, or odontoid-semiporoid hymenophore, a monomitic hyphal structure, and generative hyphae bearing clamp connections, the presence of cystidia and hyphidia in some species, thin-walled, smooth, inamyloid, and acyanophilous basidiospores. In addition, four new species, namely, Trechispora dentata, Trechispora dimitiella, Trechispora fragilis, and Trechispora laevispora, are described and illustrated. In addition, three new combinations, namely, Brevicellicium daweishanense, Brevicellicium xanthum, and Sertulicium limonadense, are also proposed.
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Due to high humidity, the ageing of room temperature vulcanized silicone rubber (RTV) has been a serious problem in Southwestern China. In order to solve the problem of RTV life prediction, the aging classification method was established by analyzing the microtopography of RTV samples in this paper. Besides, the comprehensive analysis of RTV element content, partial element content ratio, and major chemical groups of RTV samples in each aging level were conducted. It is found that as the ageing level increases, the element contents of C, Si, O, Al change accordingly and the ratio of C:Si drops from 2.39 to 1.54, and absorption peaks of the chemical groups of Si-(CH3)2, Si-O-Si, Si-CH3 and C-H in CH3 decrease. This work can enrich the investigation of RTV, and may provide useful reference for performance evaluation and replacement of RTV in substations.
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Goma/química , Elastómeros de Silicona/química , Humedad , TemperaturaRESUMEN
The direct removal of heavy metal ions from acidic wastewater is a hard problem. In this study, a novel superabsorbent, polyvinyl alcohol phosphate ester (PVAP), was designed and prepared to remove Pb(II) from acidic wastewater (pH = 3). The PVAP can absorb water and swell to reach equilibrium within 30 s, which provides the conditions for ultrafast kinetic adsorption. For 100 mg/L Pb(II) solution, the adsorption reaches equilibrium within 5 min, and the removal ratio is more than 99.9% over a wide pH range of 3-6. Adsorption kinetics and isotherm data are consistent with pseudo-second-order and Langmuir model, respectively. The calculated maximum adsorption capacity for Pb(II) is 558.66 mg/g. Thermodynamic results show that the adsorption is spontaneous and exothermic process. The removal ratio for Pb(II) of PVAP still maintains above 99% after ten recycles. The PVAP can also simultaneously remove more than 97% of other heavy metal ions (Cu(II), Cd(II), Zn(II), Co(II), and Ni(II)) from an acidic solution. Moreover, the PVAP can efficiently purify simulated acid mine heavy metal wastewater, and the results meet EPA drinking water standards. The studies of X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT-IR) spectroscopy prove that the adsorption mechanism involves surface complexation. This new superabsorbent is a promising candidate for acidic heavy metal sewage disposal.
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Alcohol Polivinílico , Contaminantes Químicos del Agua , Adsorción , Concentración de Iones de Hidrógeno , Cinética , Plomo , Espectroscopía Infrarroja por Transformada de Fourier , Termodinámica , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
Human hand, foot, and mouth disease (HFMD), an important infectious disease in children, is caused mainly by enterovirus 71 (EV71) and coxsackievirus A16 (CA16). In this study, a bivalent inactivated EV71/CA16 vaccine is developed and evaluated in immunized BALB/c mice injected through the intradermal route. Q-RT-PCR detection of the mRNA of immune signal molecules in local epithelial tissues inoculated with the vaccine indicates activation of innate immunity, which includes upregulation of immune-related chemokines, interferons and CD molecules. Further, the finding that neutralizing antibodies and specific T cellular responses were elicited in adult mice after two immunizations with the vaccine at a 28-day interval, which endowed offspring mice to defend a viral challenge, suggests the successful induction of specific protective antiviral immunity. All these data suggest that immunization with this bivalent EV71/CA16 vaccine via the intradermal route elicits effective immunity against EV71 and CA16 infection.
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Enterovirus Humano A , Enterovirus , Enfermedad de Boca, Mano y Pie , Vacunas Virales , Animales , Anticuerpos Neutralizantes , Anticuerpos Antivirales , Enfermedad de Boca, Mano y Pie/prevención & control , Ratones , Ratones Endogámicos BALB C , Vacunas Combinadas , Vacunas de Productos InactivadosRESUMEN
Hydrogen evolution from biomass photoreforming has been widely recognized as a promising strategy for relieving the pressure from energy crisis and environmental pollution, as it could generate sustainable H2 and value-added bioproducts simultaneously. Combining p-type semiconductors with n-type semiconductors to form n-p heterojunction is an effective strategy to improve the photocatalytic quantum efficiency by enhancing the separation of photogenerated electrons and holes, which could greatly facilitate the realization of such biomass photorefinery concept. However, the incompact contact between the n-type and p-type semiconductors often induces the aggregation of photogenerated electrons and holes. In this work, we design and synthesize an ultrafine n-p heterojunction TiO2-NiO core-shell structure to overcome the incompact contact in the n-p interface. When the n-p heterojunction photocatalysts are evaluated for photocatalytic water splitting and biomass lignin photoreforming respectively, the as-fabricated TiO2-NiO nanocomposite with 3.25% NiO demonstrates the highest hydrogen generation of 23.5 mmol h-1 g-1 from water splitting and H2 (0.45 mmol h-1 g-1) and CH4 (0.03 mmol h-1 g-1) cogeneration with reasonable amount of fatty acids (palmitic acid and stearic acid) production from lignin photoreforming. The excellent photocatalytic activity is ascribed to the synergistic effects of high crystallinity of TiO2 ultrafine nanoparticles, core-shell structure and n-p heterojunction with NiO nanoclusters. This present work demonstrates a simple and efficient method to fabricate ultrafine n-p heterojunction core-shell structure for noble-metal free catalyst for both water splitting and biomass photoreforming.
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Lignina , Titanio , Catálisis , HidrógenoRESUMEN
Infection with human norovirus (HuNoV) is considered a common cause of foodborne illness worldwide. Foodborne HuNoV outbreaks may result from consumption of food contaminated by an infected food handler in the foodservice environment, in which bare-hand contact, lack of hand washing, and inadequate cleaning and disinfection are common contributing factors. The goal of this study was to examine cross-contamination of a HuNoV surrogate, murine norovirus (MNV-1), during common procedures used in preparing fresh produce in a food service setting, including turning water spigots, handling and chopping Romaine lettuce, and washing hands. MNV-1 transfer % was log-transformed to achieve a normal distribution of the data and enable appropriate statistical analyses to be performed. MNV-1 transfer coefficients varied by surface type, and a greater affinity for human hands and chopped lettuce was observed. For example, greater transfer was observed from a contaminated stainless steel spigot to a clean hand (24% or 1.4-log transfer %) compared to transfer from hand to spigot (0.6% or -0.2-log transfer %). During the chopping of Romaine lettuce, MNV-1 was transferred from either a contaminated cutting board (25% or 1.4-log transfer %) or knife (~100% or 2.0-log transfer %) to lettuce at a significantly greater rate (p>0.05) than from contaminated lettuce to the board (2.1% or 0.3-log transfer %) and knife (1.2% or 0.06-log transfer %). No significant difference (p>0.05) in MNV-1 transfer coefficients was observed between bare hands and Romaine lettuce during handling. For handwashing trials, only one hand was inoculated with MNV-1 prior to washing. The handwashing methods included rubbing hands under tap water for at least 5s (average 2.8-log reduction) or washing hands for at least 20s with liquid soap (average 2.9-log reduction) or foaming soap (average 3.0-log reduction), but no statistical difference between these reductions was achieved (p>0.05). Despite the reductions of MNV-1 observed, residual virions were detected on both hands after washing in every replicate trial. This observation reveals that virions are transferred from one hand to the other during washing with and without soap. Each transfer scenario was repeated at least 9 times, and the data gathered indicate that MNV-1 transfers readily between common surfaces during food preparation. These data are important for the development of quantitative risk analyses, and will assist in the development of appropriate intervention strategies for enteric viruses in food preparation.
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Manipulación de Alimentos/normas , Microbiología de Alimentos , Lactuca/virología , Norovirus/fisiología , Infecciones por Caliciviridae/prevención & control , Enfermedades Transmitidas por los Alimentos/prevención & control , Desinfección de las Manos , Humanos , Acero InoxidableRESUMEN
OBJECTIVE: To review the current crosslinking strategies for acelluar matrix scaffold, laying the foundation for subsequent experiment. DATA SOURCES: Data were mainly obtained from recent papers published in PubMed or indexed by Web of Science, with keyword like crosslinking. RESULTS: Various crosslinking strategies, including chemical, physical and biological methods, have been introduced to facilitate the performance of fresh acellular matrix. Chemical crosslinking reagents, involved in synthetic and naturally derived agents, need to be eliminated before implantation in case of their potential biotoxicity, although several crosslinking agents with less toxicity and specific characteristics have been developed. Physical crosslinking methods present to be safe, additive-free and relatively controllable for rapid surface functionalization with no consideration of remaining radioactivity. Biological crosslinking strategies have attracted great interest, and have been demonstrated to enhance collagen-based crosslinking since their preparations do not need toxic or potentially biologically contaminated substances and can be carried out under physiological conditions. CONCLUSIONS: Kinds of crosslinking methods with its potential advantages have been developed to modify raw acelluar matrix, of which the performance are promising after being crosslinked by several crosslinking treatments. Further preclinical and clinical evaluations should be taken to vertify their safety and efficacy for the tissues and organs substitutes in tissue and regenerative medicine.
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Ingeniería de Tejidos/métodos , Materiales Biocompatibles/química , Reactivos de Enlaces Cruzados , Humanos , Andamios del Tejido/químicaRESUMEN
Coxsackie A virus is one of the major pathogens associated with hand, foot and mouth disease (HFMD). The etiological characteristics of Coxsackie A virus type 16 (CA16) are thought to correlate with the pathological process of its infection. Two CA16 strains that were isolated from a severe HFMD patient presented with different plaque forms. This observation, along with biological analysis, indicated that the differences in the strains' biological characteristics, such as proliferation kinetics and immunogenicity, correlate with differences in their pathogenicity toward neonatal mice. Furthermore, these differences are thought to be associated with the sequence of the 5' non-coding region of the viral genome and the VP1 structural region sequence. The results suggest that the biological and genetic characteristics of the CA16 viral strains are relevant to their pathogenicity.
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Enterovirus Humano A/genética , Enterovirus Humano A/patogenicidad , Enfermedad de Boca, Mano y Pie/virología , Regiones no Traducidas 5'/genética , Animales , Animales Recién Nacidos , Chlorocebus aethiops , Enterovirus Humano A/clasificación , Genoma Viral/genética , Genotipo , Humanos , Ratones , Filogenia , Especificidad de la Especie , Células Vero , Proteínas Estructurales Virales/genética , Virulencia/genéticaRESUMEN
In this work, a highly selective sample cleanup procedure that combining molecular imprinting technique (MIT) and solid phase extraction (SPE) was developed for the isolation of domoic acid (a fascinating marine toxin) from seafood samples. The molecular imprinting polymer (MIP) for domoic acid was prepared using 1,3,5-pentanetricarboxylic acid as the template molecule instead of domoic acid. 4-Vinyl pyridine was used as the functional monomer and ethylene glycol dimethacrylate was used as the cross-linking monomer. The obtained imprinted polymer showed high affinity to domoic acid and was used as selective sorbent for the SPE of domoic acid from seafood samples. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by high-performance liquid chromatography (HPLC) with diode-array detection for the detection of domoic acid was also established. Good linearity was obtained from 0.5 mg L(-1) to 25 mg L(-1) (R(2)>0.99) with a quantitation limit of 0.1 mg L(-1), which was sufficient to determine domoic acid at the maximum level permitted by several authorities. The mean recoveries of domoic acid from mussel extracts were 93.4-96.7%. It was demonstrated that the proposed MISPE-HPLC method could be applied to direct determination of domoic acid from seafood samples.
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Bivalvos/química , Cromatografía Líquida de Alta Presión/métodos , Ácido Kaínico/análogos & derivados , Polímeros/química , Alimentos Marinos/análisis , Animales , Ácido Kaínico/aislamiento & purificación , Límite de Detección , Reproducibilidad de los Resultados , Extracción en Fase SólidaRESUMEN
In this paper, a highly selective sample cleanup procedure combining molecular imprinting and solid-phase extraction (MI-SPE) was developed for the isolation of melamine in dairy products. The molecularly imprinted polymer (MIP) was prepared using melamine as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The melamine imprinted polymer was used as selective sorbent for the solid-phase extraction of melamine from dairy products. An off-line MI-SPE method followed by high-performance liquid chromatography with diode-array detection for the detection of melamine was also established. The mean recoveries of melamine from ultra-heat treatment (UHT) milk and milk powders were 92.9-98.0% and 91.6-102.8%, respectively. Good linearity was obtained from 0.5 microM to 10 microM (r>0.999) with a quantitation limit of 0.5 micromol/L (0.06 ppm) which was sufficient to analyse melamine at the maximum level permitted by U.S. Food and Drug Administration (1 ppm) in dairy products. It was demonstrated that the proposed MI-SPE-HPLC method could be applied to direct determination of melamine in dairy products.