Core-Shell Au@Nanoplastics as a Quantitative Tracer to Investigate the Bioaccumulation of Nanoplastics in Freshwater Ecosystems.

Studies on the adverse effects of nanoplastics (NPs, particle diameter <1000 nm) including physical damage, oxidative stress, impaired cell signaling, altered metabolism, developmental defects, and possible genetic damage have intensified in recent years. However, the analytical detection of NPs is still a bottleneck. To overcome this bottleneck and obtain a reliable and quantitative distribution analysis in complex freshwater ecosystems, an easily applicable NP tracer to simulate their fate and behavior is needed. Here, size- and surface charge-tunable core-shell Au@Nanoplastics (Au@NPs) were synthesized to study the environmental fate of NPs in an artificial freshwater system. The Au core enables the quantitative detection of NPs, while the polystyrene shell exhibits NP properties. The Au@NPs showed excellent resistance to environmental factors (e.g., 1% hydrogen peroxide solution, simulating gastric fluid, acids, and alkalis) and high recovery rates (>80%) from seawater, lake water, sewage, waste sludge, soil, and sediment. Both positively and negatively charged NPs significantly inhibited the growth of duckweed (Lemna minor L.) but had little effect on the growth of cyanobacteria (Microcystis aeruginosa). In addition, the accumulation of positively and negatively charged NPs in cyanobacteria occurred in a concentration-dependent manner, with positively charged NPs more easily taken up by cyanobacteria. In contrast, negatively charged NPs were more readily internalized in duckweed. This study developed a model using a core-shell Au@NP tracer to study the environmental fate and behavior of NPs in various complex environmental systems.

Effects of size and surface charge on the sedimentation of nanoplastics in freshwater.

The environmental issues caused by nanoplastics (NPs) are increasingly noticeable. Environmental behavior study of the NPs could provide vital information for their environmental impact assessment. However, associations between NPs' inherent properties and their sedimentation behaviors were seldom investigated. In this study, six types of PSNPs (polystyrene nanoplastics) with different charges (positive and negative) and particle sizes (20-50 nm, 150-190 nm and 220-250 nm) were synthesized, and their sedimentations under different environmental factors, (e.g., pH value, ionic strength (IS), electrolyte type and natural organic matter) were investigated. Results displayed that both particle size and surface charge would affect the sedimentation of PSNPs. The maximum sedimentation ratio of 26.48% was obtained in positive charged PSNPs with size of 20-50 nm, while the minimum sedimentation ratio of 1.02% was obtained in negative charged PSNPs with size of 220-250 nm at pH 7.6. The pH value shift (range of 5-10) triggered negligible changes of sedimentation ratio, the average particle size and the Zeta potential. Small size PSNPs (20-50 nm) showed higher sensitivity to IS, electrolyte type and HA condition than large size PSNPs. At high IS value ( [Formula: see text]  = 30 mM or ISNaCl = 100 mM), the sedimentation ratios of the PSNPs all increased differently according to their properties, and the sedimentation promoting effect of CaCl2 was more significant on negative charged PSNPs than positive charged PSNPs. When [Formula: see text] increased from 0.9 to 9 mM, the sedimentation ratios of negative charged PSNPs increased by 0.53%-23.49%, while that of positive charged PSNPs increased by less than 10%. Besides, humic acid (HA) addition (1-10 mg/L) would lead to a stable suspension status for PSNPs in water with different degree and perhaps different mechanism due to their charge characteristics. These results showed new light on influence factor studies of NPs' sedimentation and would be helpful for further knowledge of NPs' environmental behaviors.

Effects of nanoplastics on microalgae and their trophic transfer along the food chain: recent advances and perspectives.

Nanoplastics (NPs) have drawn increasing attention in recent years due to their potential threats to aquatic ecosystems. Microalgae are primary producers, which play important roles in the normal functioning of ecosystems. According to the source of production and laboratory experiments, both NPs and microalgae are likely to be widely found in various water environments, so they have a great chance of interacting with each other. Although tremendous efforts have been made to explore these potential interactions, a timely and critical review is still missing. In this paper, the effects of NPs on microalgae and their trophic transfer along the food chain are summarized. The toxic impact of NPs on microalgae is tightly associated with the concentrations, sizes and surface charge of NPs, as well as the microalgal species. In addition, NPs could also interact with many other contaminants, thus leading to combined effects on microalgae. NP exposure might block substance and energy exchange between microalgae and their surrounding environment, lead to a shading effect on microalgae, promote the production of reactive oxygen species (ROS) or induce direct physical damage on microalgae, thereby inhibiting the growth of microalgae. Moreover, NPs could also be trophically transferred along the food chain through microalgae and subsequently affect the species at a higher trophic level. Yet importantly, current understanding of the interactions between NPs and microalgae is still quite limited, and needs to be further studied.