RESUMEN
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 µg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 µg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Asunto(s)
Proteínas Hedgehog/metabolismo , Hueso Paladar/embriología , ARN Interferente Pequeño/genética , Transducción de Señal , Animales , Proteínas Hedgehog/genética , Factores de Transcripción de Tipo Kruppel/genética , Factores de Transcripción de Tipo Kruppel/metabolismo , Ratones , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/metabolismo , Hueso Paladar/metabolismo , ARN Interferente Pequeño/metabolismo , Proteína Gli2 con Dedos de Zinc , Proteína Gli3 con Dedos de ZincRESUMEN
A laboratory-scale fluidized-bed gasifier was designed and used to investigate the characteristics of waste tires gasification. Granulated tires were gasified with different excessive air ratios at a temperature range of 400-700 degrees C. The gasification efficiency, carbon conversion efficiency, heating value, yield and components of syngas were analyzed. Results showed that the optimum operation conditions were achieved when the gasification temperature was 700 degrees C and the excessive air radio (EAR) was 0.4. A gaseous product, mainly containing CH4, CO, H2, C2H6, and longer-chain hydrocarbon with a lower heating value (LHV) of about 4804 kJ/m3, can be generated at the highest gasification efficiency of 47.96% under the optimum operation conditions.