Expression of 20 SCPP genes during tooth and bone mineralization in Senegal bichir.

The African bichir (Polypterus senegalus) is a living representative of Polypteriformes. P. senegalus possesses teeth composed of dentin covered by an enameloid cap and a layer of collar enamel on the tooth shaft, as in lepisosteids. A thin layer of enamel matrix can also be found covering the cap enameloid after its maturation and during the collar enamel formation. Teleosts fish do not possess enamel; teeth are protected by cap and collar enameloid, and inversely in sarcopterygians, where teeth are only covered by enamel, with the exception of the cap enameloid in teeth of larval urodeles. The presence of enameloid and enamel in the teeth of the same organism is an opportunity to solve the evolutionary history of the presence of enamel/enameloid in basal actinopterygians. In silico analyses of the jaw transcriptome of a juvenile bichir provided twenty SCPP transcripts. They included enamel, dentin, and bone-specific SCPPs known in sarcopterygians and several actinopterygian-specific SCPPs. The expression of these 20 genes was investigated by in situ hybridizations on jaw sections during tooth and dentary bone formation. A spatiotemporal expression patterns were established and compared with previous studies of SCPP gene expression during enamel/enameloid and bone formation. Similarities and differences were highlighted, and several SCPP transcripts were found specifically expressed during tooth or bone formation suggesting either conserved or new functions of these SCPPs.

[Mothers' responses to teething in children in Ferlo, Senegal]. / Réponses thérapeutiques des mères face à l'éruption dentaire chez les enfants du Ferlo au Sénégal.

INTRODUCTION: Tooth eruption is a process that begins in infancy and continues throughout childhood and adolescence. This complex phenomenon induces systemic disorders requiring specific management. The objective of this study was to identify the therapeutic responses to teething in children in Ferlo, Senegal. METHODS: This qualitative study focused on mothers of teething children (infants and young children). Information was collected by individual interviews (20 persons) and two focus groups (six persons/group) concerning the symptoms and signs of teething, the first-line solution in the presence of such signs, the type and form of prevention and the mother's psychological aspects during this period. RESULTS: The methods used by mothers to treat signs of teething were incantations on strings of knots, amulets, plants and various hard objects such as coins, cowry shells, or wild donkey teeth. CONCLUSION: These methods responded to the mothers' concerns to prevent and treat the signs accompanying teething. However, mass communication sessions may be necessary to encourage visits to the dentist to improve the management of complications.

Mitogenomic phylogeny of cone snails endemic to Senegal.

Cone snails attain in Senegal one of their highest peaks of species diversity throughout the continental coast of Western Africa. A total of 15 endemic species have been described, all placed in the genus Lautoconus. While there is ample data regarding the morphology of the shell and the radular tooth of these species, virtually nothing is known regarding the genetic diversity and phylogenetic relationships of one of the most endangered groups of cones. In this work, we determined the complete or near-complete (only lacking the control region) mitochondrial (mt) genomes of 17 specimens representing 11 endemic species (Lautoconus belairensis, Lautoconus bruguieresi, Lautoconus cacao, Lautoconus cloveri, Lautoconus cf. echinophilus, Lautoconus guinaicus, Lautoconus hybridus, Lautoconus senegalensis, Lautoconus mercator, Lautoconus taslei, and Lautoconus unifasciatus). We also sequenced the complete mt genome of Lautoconus guanche from the Canary Islands, which has been related to the cones endemic to Senegal. All mt genomes share the same gene arrangement, which conforms to the consensus reported for Conidae, Neogastropoda and Caenogastropoda. Phylogenetic analyses using probabilistic methods recovered three major lineages, whose divergence coincided in time with sea level and ocean current changes as well as temperature fluctuations during the Messinian salinity crisis and the Plio-Pleistocene transition. Furthermore, the three lineages corresponded to distinct types of radular tooth (robust, small, and elongated), suggesting that dietary specialization could be an additional evolutionary driver in the diversification of the cones endemic to Senegal. The reconstructed phylogeny showed several cases of phenotypic convergence (cryptic species) and questions the validity of some species (ecotypes or phenotypic plasticity), both results having important taxonomic and conservation consequences.

[Epidemiological, clinical and forensic physical violence against women in Tambacounda (Senegal)]. / Aspects épidémiologiques, cliniques et judiciaires des violences physiques faites aux femmes dans la région de Tambacounda (Sénégal).

INTRODUCTION: To contribute to the fight against physical violence against women, this work aimed to make a 10-year review of cases treated at the Regional Court of Tambacounda. METHODOLOGY: This observational, retrospective, descriptive and analytical study was conducted from 15 October 2014 to 15 April 2015. It covered all the court records of victims of intentional injury from 2006 to 2015. The data was entered and analyzed using Epi info 3.3.2 software. RESULTS: In all, 113 cases were treated. The mean age was 26.5±10.08 years for victims versus 32.5±13.8 years for the aggressors. The victims were all female, and 77.9% of offenders were male. The assaults took place mostly during the day (57.5%), and especially in the homes of victims (61.0%), in the street (16.8%) in the bush (12.4%). The consultation period was less than 24hours for 54.9% of the victims. Clinical examination differentiated: fractures (15%); contusions (13.5%); penetrating wounds (10.6%); bruises (9.7%); eye injuries (7.9%); broken teeth (7.9%); diffuse pains (7.1%), 6.2% polytrauma (6.2%), and 5.3% of trauma on pregnancies. Among the violence, 33.6% were domestic; 11.5% were associated with rape (7.1%); psychological violence (3.6%); rape and death threat (1.8%). Rape was statistically more common among child victims [OR=10.7 (3.2-35.5)] and/or educated victims [OR=5.8 (1.7-19.9)] and aggression in the bush [OR=7.5 (2.2 to 14.2)]. The attackers were lonely and imprisoned in 94.7% and 73.5% of cases respectively. The sentence was firm imprisonment for 89.2% of cases. CONCLUSION: In Tambacounda, health and judicial authorities should enhance public awareness concerning the risk of violence. The extension of this type of study to the national level would have enabled better orientation of control strategies against this scourge.

[Oral health status, supply of oral and dental care among Senegalese children: a review of available data]. / État de santé, offre et recours aux soins bucco-dentaires chez les enfants sénégalais?: synthèse des données disponibles.

INTRODUCTION: Oral and dental diseases are a major public health problem due to their high prevalence and their impact on general health. OBJECTIVE: This article reviews the oral health status and oral health care supply and uptake in children. METHOD: The available literature were reviewed. The Medline and Embase databases, and the Senegal Ministry of Health and Social Action and National Agency of Statistics and Demography (ANSD) websites were consulted. A total of 7 articles, 3 PhD theses and 8 reports were selected for analysis. The review was supplemented by interviews. RESULTS: Overall, children have poor oral health status. Health care supply is insufficient (1 dentist/38,000 residents) and poorly distributed (mostly in the capital) with unequal oral health care uptake. CONCLUSION: This systematic review of the literature revealed a lack of reliable data on the oral health of children. It also concluded on a generally poor oral health status.

Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus.

BACKGROUND: Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence. METHODS: We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5% Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates. RESULTS: All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50% of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva. CONCLUSION: All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal.

Stroke and periodontal disease in Senegal: case-control study.

OBJECTIVE: To determine the periodontal factors associated with stroke in melanodermic subjects in Senegal. STUDY DESIGN: Case-control study. METHODS: One hundred and twenty cases and 120 controls were included in this study. Cases had been diagnosed with stroke by a neurologist, with the diagnosis confirmed by scanner. Controls had never had any type of stroke. Data were collected regarding sociodemographic characteristics, lifestyle behaviours, general history, type of stroke (ischaemic or haemorrhagic) and periodontal parameters [plaque index, papillary bleeding index, pocket depth, clinical attachment loss, Community Periodontal Index of Treatment Needs and periodontitis (defined by clinical attachment loss >2 mm and pocket depth >3 mm)]. Logistic regression analysis was performed using R software to isolate a final model after adjustment for the 5% threshold. RESULTS: All periodontal characteristics were more common among cases than among controls. Periodontitis (odds ratio 1.58, 95% confidence interval 1.1-3.022) and periodontal parameters were significantly associated with stroke, adjusted for hypertension, sedentary lifestyle, and the interaction between periodontitis and age. CONCLUSIONS: Periodontal disease is associated with stroke in the Senegalese population. Prospective longitudinal studies should be undertaken to improve understanding.

[Sagittal photogrammetric evaluation of the soft tissue profile between two different racial groups: a comparative study]. / Caractéristiques photogrammétriques sagittales de profil des tissus mous chez deux groupes raciaux. Etude comparative.

INTRODUCTION: Facial soft-tissue analyses made from photographic records (photogrammetric analysis of the soft tissues) showed interracial dimorphism. Standard of these facial analyses, originally obtained from Caucasian might not be appropriate for the diagnosis and treatment planning of other groups of orthodontic patients. The purpose of this study was to compare the sagittal photogrammetric soft tissue profile characteristics between Senegalese and Moroccan adults. MATERIALS AND METHODS: A cross-sectional study was performed in a group of Senegalese and Moroccan students in the Faculty of Medicine, Pharmacy and Dentistry of Dakar. Standardized facial-profile photographs were taken with each student in natural head position, centric relation, and relaxed lip posture. Twenty four linear parameters were measured on paper sheet using a graduated ruler. For each variable mean and SD were calculated. In addition independent samples t-test was performed to detect sexual and racial dimorphism. Results were regarded as significant at p = 0.05. RESULTS: Senegalese subjects had significantly more lips protrusion than Moroccan who had significantly a more elongated and more anterior positioned nose, a more chin prominence and a tendency to cutaneous class II than Senegalese. CONCLUSION: Further studies including different age groups subjects would allow having longitudinal data according to age.

Knowledge of and management attitude regarding dentin hypersensitivity among dentists from a West African country.

AIM: The aim of the present study was to assess knowledge of, and management attitude of dentists regarding dentin hypersensitivity. MATERIALS AND METHODS: The study involved all the dentists from private and public sectors, exerting in Senegal. The following data were requested from the surveyed dentists using an anonymous questionnaire; sociodemographics (i.e. age, gender, area of activity, etc.) and knowledge on triggering factor, type of pain, diagnosis, preventive and curative procedures. RESULTS: Out of the 238 dentists who received the questionnaire, 68.9% returned properly filled forms. They were 116 males and 48 females with a mean age of 41.99 ± 8.50 years. Eighty three percent of the participants had a good understanding of the characteristics of pain related to DH and 92% recognized chemical and thermal stimuli as triggering factor while mechanical stimulus was not evoked. Many responders (90.9%) did not have any idea of the mechanism for pain transmission across the dentin. Regarding diagnosis technique, 68% use mechanical stimuli to elicit DH pain. Regarding management procedure, the use of desensitizing tooth paste is the mostly chosen option followed by professional topical application of fluoride. More than 1/3rd of the surveyed dentists confess resorting to root canal to manage DH. CONCLUSION: We recommend incorporation of basic science knowledge on orofacial pain and competencies to manage painful conditions like dentin hypersensitivity. Also, health regulatory institutions should make continuing dental education a requirement to preserve the dental licensure.

[Prevalence of dental caries: national pilot study comparing the severity of decay (CAO) vs ICDAS index in Senegal]. / Prévalence de la carie dentaire: étude pilote nationale comparant l'indice de sévérité de la carie (CAO) vs ICDAS au Sénégal.

UNLABELLED: This pilot study has for main objective to measure the applicability and the utility of ICDAS index in a context of prevention in developing countries. Dental caries prevalence was evaluated among schoolchildren using DMF (WHO basic method) vs. ICDAS index in Senegal. MATERIALS AND METHOD: A representative stratified random cluster sample of 677 primary and college schoolchildren aged 12 and 15 years was examined for caries prevalence. The clinical examination was conducted in two steps for each. The investigator proceeded at first to the inventory of the number of teeth decayed (D), missing (M) or filled (F) according to the WHO basic method. Then, after cleaning and drying all teeth, a two-digit ICDAS code was used to record data at each dental surface. RESULTS: Caries prevalence (96%) was higher than expected in Senegal. ICDAS index provides 43% moreover information than DMF. The need for prevention (ICDAS1: 66%/72% and ICDAS2: 54%/58%) and intercept (ICDAS3: 40%/42% and ICDAS4: 31%/33%) are higher than the need of curative treatment (ICDAS5: 18%/23% and ICDAS6: 27%/33%) respectively among primary and college schoolchildren. CONCLUSION: Preventive programs are urgently needed in Senegal. It's necessary to lead epidemiological studies in other African countries for determining caries prevalence using the ICDAS criteria to harmonize oral health regional planning.

[Relationship between bizygomatic width and the size of maxillary anterior teeth among young Senegalese black people recruited in army]. / Corrélation entre la distance bizygomatique et la largeur des dents antéromaxillaires chez les jeunes sénégalais mélanodermes recrutés dans le service militaire.

INTRODUCTION: Prosthetic teeth size and placement must be in perfect harmony with the bucco-facial structures for a successful and esthetic removable prosthesis. This study aimed to determine among young Senegalese black people facial index estimating maxillary central incisor width and distance between canine points starting from bizygomatic width. PATIENTS AND METHODS: 621 black Senegalese participated to this transversal study. Measurements were made using anthropometric slide caliper and electronic slide caliper. The comparative analysis was carried out by the test t of Student. The correlative analysis used the chi-square test. The risk of error was fixed at 5%. RESULTS: Positive correlations were found between variables. Distance between canine points was significantly (p = 0.000) correlated to bizygomatic width and central incisor width. Using bizygomatic width, the average dividing factors to estimate central incisor width and distance between canine points were respectively 15.13 and 3.75. CONCLUSION: Distance between canine points and central incisor width can be given starting from bizygomatic width, as guide of selecting maxillary anterior prosthetic teeth width.

[Prevalence of dental caries among 12-year old schoolchildren in the Dakar region]. / Prévalence de la carie dentaire chez les élèves de 12 ans de la région de Dakar.

Oral diseases are considered as major public health problem. Dental caries restricts the school activities and are the dominant cause of dental morbidity. The aims of this survey were to determine prevalence of dental caries among pupils of Dakar and to study their food habits and oral hygiene. A descriptive transversal study was conducted from February 15th to May 07th, 2011 with respect of WHO directives (protocol of 1997) readjusted to Senegalese context. Our results showed a prevalence of 41.8%; a mean DMF index of 0.644; a proportion of teeth decayed of 65%, 32.2% for missing teeth and 2.7% of filled teeth. The multivariate logistic regression analysis showed that snacking, consumption of sweetened drinks far from meals and brushing teeth before the meals were significantly linked to the presence of dental caries. These results showed that the state of oral health and the prevention level of dental caries among these children were not so good. A new orientation of oral health policy based on messages regarding the main etiologic factors is imperative.

Socio-cultural aspects of oral health among the Fulani in Ferlo (Senegal): a qualitative study.

OBJECTIVES: Health is a subjective concept that considers the social, cultural, environmental and behavioural problems of the individual. This study was conducted with the objective of better understanding the sociocultural aspects related to the oral health of the Fulani populations of Ferlo, Senegal. METHODS: The study was qualitative and based in the area of the Great Green Wall (GGW) in the region known as Ferlo, northern Senegal. Data were collected by semi-structured interviews in a sample of the population and through discussions with a focus group. It concerned health and aesthetics of the teeth, care and traditional herbal recipes, teeth and superstitions. RESULTS: It appears that people were using the toothpick, the chewing stick and/or charcoal to clean their teeth. Confusion persisted with respect to the types of food consumed that were implicated in the occurrence of dental caries: tea, rice, 'jumbo' (a seasoning spice) and tobacco. 'Borom bop', which means 'master of the head', was the most commonly reported cause of caries. Healthy, beautiful teeth were attributes of beauty and elegance, enhanced by tattoos and crafted crowns in the Fulani. Their health problems were generally managed by healers or traditional practitioners who based their practices on empirical and 'handed down' knowledge. Socio-anthropological meanings were given to children with neonatal teeth. CONCLUSIONS: It is therefore important to consider the sociocultural aspects in oral health projects and programmes; the place of herbal medicine in dentistry should be recognized and maybe researched in the region of the Great Green Wall.

A study of cephalometric soft tissue profile among adolescents from the three West African countries of Nigeria, Ghana and Senegal.

BACKGROUND: Since the introduction of cephalometry, numerous studies have established normal values for Caucasian populations. In Africa, most investigations have established norms and ethnic variations associated with the skeletal pattern. To date, there has been no study comparing soft tissue patterns among adolescents in the West African sub-region. OBJECTIVES: The objective of this investigation was to determine and compare soft tissue patterns among 12- to 16-year-old Nigerian, Ghanaian and Senegalese adolescents, establish any gender dimorphism and compare them with published Caucasian norms. METHODOLOGY: Lateral cephalometric radiographs of adolescents with a normal incisor relationship aged between 12 and 16 years from Nigeria, Ghana, and Senegal were taken under standardized conditions and traced to determine soft tissue patterns. Data obtained were subjected to statistical analysis. RESULTS: The total sample consisted of 165 females and 135 males with a mean age of 13·96 (1·58) years. A number of soft tissue parameters showed significant differences (P<0·05). These included comparison between males and females, and Nigerian, Ghanaian and Senegalese, including lip separation, upper lip length, upper lip exposure, Li-esthetic line, lower lip-NP, nasal tip angle, N-Pr-Pg, Pg-Ls, B-N pogonion and pogonion-mandibular angle. Differences also existed between these West African soft tissue values and published Caucasian norms, including nasolabial angle, mentolabial angle, nasal depth, nose tip, total soft tissue facial convexity and nasal depth angle. CONCLUSIONS: The comparative analysis of soft tissue patterns among 12- to 16-year-old adolescents from Nigeria, Ghana and Senegal demonstrated statistically significant differences in soft tissue value between these West African adolescents and published Caucasian soft tissue norms. This study provides useful data in relation to soft tissue parameters for subjects originating from the West African sub-region.

[Pharmacovigilance among dentists: a survey of practitioners in Dakar, Senegal]. / Pharmacovigilance chez les chirurgiens-dentistes: enquête dans la région de dakar, Sénégal.

AIM: Since 1968, the World Health Organization has used an international pharmacovigilance programme to monitor and examine the effects of drugs. The purpose of this study was to assess the knowledge and attitudes of dentists toward pharmacovigilance. METHODS: The paper presents the results of a cross-sectional descriptive study of 103 dentists in Senegal, based on an exhaustive selection process. Data were collected on the dentists' sociodemographic characteristics and their knowledge and attitudes toward pharmacovigilance. RESULTS: Over 50% of the sample were general practitioners. The average number of years of professional experience was 9 years. Painkillers (41.9%) were the most widely prescribed drugs, followed by antibiotics, which were particularly involved in the onset of adverse effects (41.8%). Adverse effects were observed by just 3.8% of the sample. Almost 90% of the participants were not aware of the existing pharmacovigilance system. CONCLUSION: The results suggest that pharmacovigilance training and education sessions for dentists are needed to improve their knowledge of pharmacovigilance and to foster positive attitudes toward adverse effects.

[Relationships between the dental arches measurements and the peak nasal inspiratory flow (PNIF): a cross sectional study on 78 Senegalese teenagers]. / Relations entre les mensurations des arcades dentaires et le débit nasal inspiratoire de pointe: etude transversale sur 78 adolescents Senegalais.

INTRODUCTION: Upper airways obstruction can result in the reduction of the transversal dimensions of the maxillary arch which affect the vertical and the sagittal dimensions. The PNIF measure is an objec- tive and cheap way to assess the upper airways permeability. The aim of this study was to determine the relationship between PNIF and dental arch sizes. MATERIALS AND METHODS: Seventy eight patients (40 girls and 38 boys) age ranged 10-15 years were included in this cross sectional study. The PNIF was measured with a Youlten peak flow meter (Clement Clarke International, London, United Kingdom). The sagittal, vertical and transversal dimensions of the dental arches were evaluated on dental casts with a digital caliper (Mitutoyo Corporation, Tokyo, Japan). T test was used to assess differences in PNIF and dental arches variables between subjects grouped according to sex (male and female). The Pearson's correlation coefficient was used to analyze the relationship between the dental arches sizes and the PNIF. The significance was set at p < 0.05. RESULTS: PNIF rate was higher in girls than in boys but the difference was not significant. There were sexual dimorphism regarding the anterior and total lengths of the upper arch, the total length of the lower arch and the maxillary intermolar width which are significantly more important for boys (p respectively equal to 0.05; 0.03; 0.04 and 0.04). The PNIF was significantly and negatively correlated with the total length of the upper arch (r = -0.25). The others measurements did not show significant correlation with PNIF. CONCLUSION: Others parameters are needed to complement the peak nasal inspiratory flow rate for the respiratory disorders related dental arches sizes abnormalities diagnosis.

[Digestive pathology and oral condition in the rural populations of the Ferlo in Senegal]. / Pathologie digestive et état bucco-dentaire chez les populations rurales du Ferlo au Sénégal.

UNLABELLED: The aim of this work was to study the association between dental and periodontal status and digestive pathology in the Ferlo populations in Senegal. METHOD: This was a cross sectional study involving 300 people living in the area of the Ferlo in the Centre East of Senegal. Selected individuals must be over the age of 15 years and resided in the area. Cluster sampling helped recruit individuals. Collected information related to digestive pathology (yes or no), periodontal status (plate index, inflammation, bleeding, loss of attachment, the depth of pocket and the CPITN.) Dental status was evaluated by the DMFT. The data analyzed with software R and the Student's t test used to compare the averages of the indices in both groups at the threshold of 5%. Multivariate logistic regression analysis was used to isolate the final model with a threshold of 5%. RESULTS: Averages of dental and periodontal index were broadly equal in both groups (sick and not sick). The DMF and missing teeth were significantly associated with the digestive pathology after adjustment on gender, age, BMI, marital status, the number of cigarettes smoked and the depth of pocket. CONCLUSION: This study revealed a significant association between dental status and digestive pathology. The comprehensive care of patients therefore raises the interest of collaboration between surgeons, dentists and gastroenterologists for effective and adequate treatment.

HIV shedding in the oral cavity: an assessment of HIV type, immunovirologic, demographic and oral factors.

OBJECTIVE: To quantify the prevalence and burden of HIV type 2 (HIV-2) and HIV-1 RNA in the oral cavity of antiretroviral therapy-naive HIV-infected Senegalese individuals and to identify correlates of oral HIV viral loads. DESIGN: A cross-sectional study of 163 HIV-1 and 27 HIV-2-infected antiretroviral therapy-naive Senegalese adults. METHODS: Participants received clinical and oral exams and provided blood and oral wash samples for viral load and plasma CD4 count ascertainment. Logistic and interval regression models were used to identify univariate and multivariable associations between presence and level of oral HIV RNA and various immunovirologic, local and demographic factors. RESULTS: Presence of detectable oral HIV RNA was less common in HIV-2-infected compared with HIV-1-infected study participants (33% vs 67%, OR 0.25, 95% CI 0.11 to 0.59). HIV type was no longer associated with oral shedding of HIV when plasma viral load was considered. Detection of oral HIV RNA was associated with increased plasma viral load in both HIV-1-infected and HIV-2-infected individuals (HIV-1, OR 1.89, 95% CI 1.24 to 2.61; HIV-2, OR 1.93, 95% CI 1.1 to 3.39). Oral HIV-1 detection was also associated with periodontal disease (OR 3.02, 95% CI 1.16 to 7.87). CONCLUSIONS: Oral shedding of HIV-2 RNA is less common than HIV-1 RNA, a likely consequence of lower overall viral burden. Both systemic and local factors may contribute to shedding of HIV in the oral cavity.

Reconstruction of periodicity of repetitive linear enamel hypoplasia from perikymata counts on imbricational enamel among dry-adapted chimpanzees (Pan troglodytes verus) from Fongoli, Senegal.

Periodicity of repetitive linear enamel hypoplasia (rLEH) in apes from high latitudes with single wet and dry seasons annually has not been described. We reconstruct periodicity and duration of rLEH in canine teeth from three recently deceased chimpanzees from Fongoli, Senegal with a marked seven-month dry season. High-resolution dental molds were taken in the field for magnified imaging with digital microscopy. Photomontages allowed counting of perikymata between episodes of rLEH for reconstruction of periodicity and duration of physiological stress. Where rLEH spans the imbricational enamel, the number of events is consistent with years required to form canine imbricational enamel; i.e., periodicity of rLEH seems circannual. We predicted perikymata counts between rLEH events ranging from 52 to 61 based on reported "long counts" of 7-6 days. Counts ranged from 29.5 to 44, individual mean of 36.7. This discrepancy could be explained by recurrent stress with a periodicity of 7.2-8.4 months, or by long counts of 10 days per stria. Neither is supported in the literature. Since we find evidence of rLEH with circannual periodicity, we postulate the existence of non-emergent imbricational striae. Based on evidence that stress at Fongoli recurs annually, we reconstruct stress duration of 2-3 months, longer than reported for chimpanzees living in other habitats, which we attribute to heat stress and food shortage near shrinking waterholes. We conclude that canine teeth from a small mortality cohort of chimpanzees at Fongoli preserve a faithful record of dry season stress in an extreme environment.

Prevalence of necrotizing ulcerative gingivitis and associated factors in Koranic boarding schools in Senegal.

BACKGROUND: Necrotizing ulcerative gingivitis is the most common clinical syndrome preceding noma. It is found in developing countries and in malnourished children and especially in deprived groups such as children at Koranic boarding schools. The objective of this study was to determine the prevalence of necrotizing ulcerative gingivitis and factors associated with its occurrence in a boarding school population. METHODS: This was a cross-sectional study of children in Koranic boarding schools in the city of Touba, Senegal. A multistage sampling strategy was used and 8 out of 17 schools were selected. The variables collected were gender, age, oral hygiene habits, duration of residence, presence of ulcerative gingivitis and plaque, and gingival bleeding index. A logistic regression analysis with R software using the manual procedure down was used to identify factors associated with the dependent variables. RESULTS: There were 501 participants and boys made up 92% of the study group. The mean age was 9.3 (sd 4.0) years. The mean of duration residence was 3.4 (sd 1.5) years. The prevalence of necrotizing ulcerative gingivitis was 37% and 81% of children did not use a toothbrush or a chewing-stick. The length of residence, school size, hygiene habits and plaque and bleeding indices were significantly associated with necrotizing gingivitis after adjustment for other variables. CONCLUSION: It is necessary to develop oral hygiene programs, to establish policies to manage the oral health of children and to improve health and nutrition at Koranic boarding-schools.