ABSTRACT
Dissolved organic matter (DOM) is one of the most complex, dynamic and abundant sources of organic carbon, but its chemical reactivity remains uncertain1-3. Greater insights into DOM structural features could facilitate understanding its synthesis, turnover and processing in the global carbon cycle4,5. Here we use complementary multiplicity-edited 13C nuclear magnetic resonance (NMR) spectra to quantify key substructures assembling the carbon skeletons of DOM from four main Amazon rivers and two mid-size Swedish boreal lakes. We find that one type of reaction mechanism, oxidative dearomatization (ODA), widely used in organic synthetic chemistry to create natural product scaffolds6-10, is probably a key driver for generating structural diversity during processing of DOM that are rich in suitable polyphenolic precursor molecules. Our data suggest a high abundance of tetrahedral quaternary carbons bound to one oxygen and three carbon atoms (OCqC3 units). These units are rare in common biomolecules but could be readily produced by ODA of lignin-derived and tannin-derived polyphenols. Tautomerization of (poly)phenols by ODA creates non-planar cyclohexadienones, which are subject to immediate and parallel cycloadditions. This combination leads to a proliferation of structural diversity of DOM compounds from early stages of DOM processing, with an increase in oxygenated aliphatic structures. Overall, we propose that ODA is a key reaction mechanism for complexity acceleration in the processing of DOM molecules, creation of new oxygenated aliphatic molecules and that it could be prevalent in nature.
Subject(s)
Carbon , Fresh Water , Carbon/analysis , Carbon/chemistry , Carbon-13 Magnetic Resonance Spectroscopy , Fresh Water/chemistry , Lakes/chemistry , Lignin/chemistry , Oxidation-Reduction , Oxygen/chemistry , Polyphenols/chemistry , Rivers/chemistry , Sweden , Tannins/chemistry , Carbon CycleABSTRACT
Cis-regulatory changes are thought to play a major role in adaptation. Threespine sticklebacks have repeatedly colonized freshwater habitats in the Northern Hemisphere, where they have evolved a suite of phenotypes that distinguish them from marine populations, including changes in physiology, behavior, and morphology. To understand the role of gene regulatory evolution in adaptive divergence, here we investigate cis-regulatory changes in gene expression between marine and freshwater ecotypes through allele-specific expression (ASE) in F1 hybrids. Surveying seven ecologically relevant tissues, including three sampled across two developmental stages, we identified cis-regulatory divergence affecting a third of genes, nearly half of which were tissue-specific. Next, we compared allele-specific expression in dental tissues at two timepoints to characterize cis-regulatory changes during development between marine and freshwater fish. Applying a genome-wide test for selection on cis-regulatory changes, we find evidence for lineage-specific selection on several processes between ecotypes, including the Wnt signaling pathway in dental tissues. Finally, we show that genes with ASE, particularly those that are tissue-specific, are strongly enriched in genomic regions of repeated marine-freshwater divergence, supporting an important role for these cis-regulatory differences in parallel adaptive evolution of sticklebacks to freshwater habitats. Altogether, our results provide insight into the cis-regulatory landscape of divergence between stickleback ecotypes across tissues and during development, and support a fundamental role for tissue-specific cis-regulatory changes in rapid adaptation to new environments.
Subject(s)
Smegmamorpha , Animals , Smegmamorpha/genetics , Fresh Water , Adaptation, Physiological/genetics , Genome , AcclimatizationABSTRACT
Pollution from microplastics and anthropogenic fibres threatens lakes, but we know little about what factors predict its accumulation. Lakes may be especially contaminated because of long water retention times and proximity to pollution sources. Here, we surveyed anthropogenic microparticles, i.e., microplastics and anthropogenic fibres, in surface waters of 67 European lakes spanning 30° of latitude and large environmental gradients. By collating data from >2,100 published net tows, we found that microparticle concentrations in our field survey were higher than previously reported in lakes and comparable to rivers and oceans. We then related microparticle concentrations in our field survey to surrounding land use, water chemistry, and plastic emissions to sites estimated from local hydrology, population density, and waste production. Microparticle concentrations in European lakes quadrupled as both estimated mismanaged waste inputs and wastewater treatment loads increased in catchments. Concentrations decreased by 2 and 5 times over the range of surrounding forest cover and potential in-lake biodegradation, respectively. As anthropogenic debris continues to pollute the environment, our data will help contextualise future work, and our models can inform control and remediation efforts.
Subject(s)
Lakes , Microplastics , Particulate Matter , Water Pollutants/analysis , Ecosystem , Europe , Fresh Water/chemistry , Hydrology , Plastics , WastewaterABSTRACT
Overkill of large mammals is recognized as a key driver of Pleistocene megafaunal extinctions in the Americas and Australia. While this phenomenon primarily affected mega-mammals, its impact on large Quaternary reptiles has been debated. Freshwater turtles, due to the scarcity of giant forms in the Quaternary record, have been largely neglected in such discussions. Here we present a new giant podocnemidid turtle, Peltocephalus maturin sp. nov., from the Late Pleistocene Rio Madeira Formation in the Brazilian Amazon, that challenges this assumption. Morphological and phylogenetic analyses of the holotype, a massive partial lower jaw, reveal close affinities to extant Amazonian species and suggest an omnivorous diet. Body size regressions indicate Pe. maturin possibly reached about 180 cm in carapace length and is among the largest freshwater turtles ever found. This finding presents the latest known occurrence of giant freshwater turtles, hinting at coexistence with early human inhabitants in the Amazon.
Subject(s)
Turtles , Animals , Humans , Phylogeny , Brazil , Reptiles , Fresh Water , MammalsABSTRACT
Pathogenic bacteria in drinking-water pose a health risk to consumers, as they compromise the quality of portable water. Chemical disinfection of water containing dissolved organic matter (DOM) causes harmful disinfection by-products. In this work, 4-hydroxybenzoic acid (4-HBA) blended polyethersulfone membranes were fabricated and characterised using microscopic and spectroscopic techniques. The membranes were evaluated for the removal of bacteria and DOM from synthetic and environmental water. Permeate flux increased from 287.30 to 374.60 l m-2 h-1 at 3 bars when 4-HBA increased from 0 to 1.5 wt.%, suggesting that 4-HBA influenced the membrane's affinity for water. Furthermore, 4-HBA demonstrated antimicrobial properties by inhibiting bacterial growth. The membrane with 1 wt.% 4-HBA recorded 99.4 and 100% bacteria removal in synthetic and environmental water, respectively. Additionally, DOM removal of 55-73% was achieved. A flux recovery ratio (FRR) of 94.6% was obtained when a mixture of bacteria and humic acid was filtered, implying better fouling layer reversibility during cleaning. Furthermore, 100% FRR was achieved when a multimedia granular filtration step was installed prior to membrane filtration. The results illustrated that the membranes had a high permeate flux with low irreversible fouling. This indicated the potential of the membranes in treating complex feed streams using simple cleaning protocols.
Subject(s)
Bacteria , Biofilms , Biofouling , Fresh Water , Membranes, Artificial , Water Purification , Biofilms/drug effects , Biofilms/growth & development , Biofouling/prevention & control , Water Purification/methods , Fresh Water/microbiology , Bacteria/drug effects , Humic Substances/analysis , Filtration/methods , Parabens/chemistry , Sulfones/chemistry , Polymers/chemistryABSTRACT
Microplastic has emerged as a global threat owing to its chronic ubiquity and persistence. Microplastics' small size expedites their ingestion at each trophic level causing biomagnification and bioaccumulation, which has raised public concerns. The present study isolated, quantified and characterized the abundance, shape, size, color, and chemical composition of the microplastics from water and sediments of the Hirakud Reservoir through a scanning electron microscope and FTIR. The ecological risk associated with the microplastics was assessed using the species sensitivity distribution (SSD) method to derive the Predicted No-Effect Concentration (PNEC) value and risk quotient (RQ). The abundance of microplastics in the surface water and sediments of the Hirakud Reservoir was estimated at 82-89 particles/L and 159-163 particles/kg, respectively. Fiber-shaped microplastics dominated both surface water (46.21%) and sediment samples (44.86%). Small-sized microplastics (53-300 µm) prevailed in all samples. Color delineation exhibited an abundance of transparent microplastics. Chemical characterization indicated the dominance of polypropylene (38%), followed by high-density polyethylene, low-density polyethylene, and polystyrene. The calculated PNEC value was 3,954 particles/m3, and the RQ was estimated to be 0.02073-0.04122 indicating negligible ecological risk to freshwater species in all the sampling sites.
Subject(s)
Environmental Monitoring , Microplastics , Water Pollutants, Chemical , Microplastics/analysis , Microplastics/toxicity , India , Water Pollutants, Chemical/analysis , Risk Assessment , Geologic Sediments/chemistry , Geologic Sediments/analysis , Fresh Water/chemistry , Fresh Water/analysisABSTRACT
From review of the very few topical studies to date, we conclude that while effects are variable, microplastics can induce direct ionoregulatory disturbances in freshwater fish and invertebrates. However, the intensity depends on microplastic type, size, concentration, and exposure regime. More numerous are studies where indirect inferences about possible ionoregulatory effects can be drawn; these indicate increased mucus production, altered breathing, histopathological effects on gill structure, oxidative stress, and alterations in molecular pathways. All of these could have negative effects on ionoregulatory homeostasis. However, previous research has suffered from a lack of standardized reporting of microplastic characteristics and exposure conditions. Often overlooked is the fact that microplastics are dynamic contaminants, changing over time through degradation and fragmentation and subsequently exhibiting altered surface chemistry, notably an increased presence and diversity of functional groups. The same functional groups characterized on microplastics are also present in dissolved organic matter, often termed dissolved organic carbon (DOC), a class of substances for which we have a far greater understanding of their ionoregulatory actions. We highlight instances in which the effects of microplastic exposure resemble those of DOC exposure. We propose that in future microplastic investigations, in vivo techniques that have proven useful in understanding the ionoregulatory effects of DOC should be used including measurements of transepithelial potential, net and unidirectional radio-isotopic ion flux rates, and concentration kinetic analyses of uptake transport. More sophisticated in vitro approaches using cultured gill epithelia, Ussing chamber experiments on gill surrogate membranes, and scanning ion selective electrode techniques (SIET) may also prove useful. Finally, in future studies we advocate for minimum reporting requirements of microplastic properties and experimental conditions to help advance this important emerging field.
Subject(s)
Fishes , Fresh Water , Gills , Invertebrates , Microplastics , Water Pollutants, Chemical , Animals , Gills/drug effects , Gills/metabolism , Microplastics/toxicity , Fishes/physiology , Fishes/metabolism , Water Pollutants, Chemical/toxicity , Invertebrates/drug effects , Invertebrates/physiologyABSTRACT
European sea bass (Dicentrarchus labrax [Linnaeus, 1758]) is a euryhaline marine migrant fish highly valuable for fisheries and aquaculture. Although juveniles are known to use estuaries and occasionally move to freshwater environments, these freshwater incursions had not been reported for adults. Recently, this behavior was observed in the Tagus River (Portugal) for adults occurring up to 150 km from the river mouth, about 80 km upstream from the tidal influence, suggesting the existence of a freshwater contingent. Fisheries management of sea bass should consider the putative existence of a freshwater contingent.
Subject(s)
Bass , Animals , Aquaculture , Fisheries , Fresh Water , SeafoodABSTRACT
Microplastic (MP) pollution has become a pressing concern in global freshwater ecosystems because rivers serve as essential channels for the transport of terrestrial debris to the ocean. The current researches mostly focus on the large catchments, but the impact on the small catchments remains underexplored. In this study, we employed Strahler's stream order classification to delineate the catchment structure of the Beijiang River in South China. The distribution pattern of MP contamination and the factors influencing the distribution pattern, were assessed across the streams at different orders. We found that the Beijiang River was moderately polluted compare to other rivers in China, with an average MP abundance of 2.15 ± 1.65 items/L. MP abundance ranged from 3.17 to 1.45 items/L in the streams at different orders, and significantly decreased with increasing stream order (R2 = 0.93). This highlights the key role of small rivers as the channels for the transport of MPs from watersheds to main streams. The high abundance of PP and PE fibers, the high correlation between the stream order and the resin proportion (R2 = 0.89), and the significant correlation between MP abundance and proximity to urban centers (P = 0.02), indicated that MP pollution across the streams at different orders was predominantly influenced by anthropogenic activities, rather than natural environmental factors. By integrating MP data with hydrographic information, the annual MP loads for the streams at Orders 1 to Order 5 were estimated to be 4.63, 39.38, 204.63, 503.06, and 1137.88 tons/yr, respectively. Additionally, an ecological risk assessment indicates that MP pollution led to a low risk in the Beijiang River. Our findings deepen the understanding of MP pollution within freshwater river networks, and emphasize the crucial role of tributary systems in transporting MPs to main river channels.
Subject(s)
Environmental Monitoring , Microplastics , Rivers , Rivers/chemistry , Microplastics/analysis , China , Fresh Water/chemistry , Water Pollutants, Chemical/analysis , EcosystemABSTRACT
Water bodies play a crucial role in supporting life, maintaining the environment, and preserving the ecology for the people of India. However, in recent decades, human activities have led to various alterations in aquatic environments, resulting in environmental degradation through pollution. The safety of utilizing surface water sources for drinking and other purposes has come under intense scrutiny due to rapid population growth and industrial expansion. Surface water pollution due to micro-plastics (MPs) (plastics < 5 mm in size) is one of the emerging pollutants in metropolitan cities of developing countries because of its utmost resilience and synthetic nature. Recent studies on the surface water bodies (river, pond, Lake etc.) portrait the correlation between the MPs level with different parameters of pollution such as specific conductivity, total phosphate, and biological oxygen demand. Fibers represent the predominant form of MPs discovered in surface water bodies, exhibiting fluctuations across seasons. Consequently, present study prioritizes understanding the adaptation, prevalence, attributes, fluctuations, and spatial dispersion of MPs in both sediment and surface water environments. Furthermore, the study aims to identify existing gaps in the current understanding and underscore opportunities for future investigation. From the present study, it has been reported that, the concentration of MPs in the range of 0.2-45.2 items/L at the Xisha Islands in the south China sea, whereas in India it was found in the range of 96 items/L in water samples and 259 items/kg in sediment samples. This would certainly assist the urban planners in achieving sustainable development goals to mitigate the increasing amount of emergent pollutant load.
Subject(s)
Environmental Monitoring , Water Pollutants, Chemical , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , India , Microplastics/analysis , Fresh Water/chemistryABSTRACT
Plastic pollution in the form of microplastics (MPs), poses a significant threat to natural ecosystems, with detrimental ecological, social, and economic impacts. This review paper aims to provide an overview of the existing research on the interaction between microbial biofilms and MPs in natural environments. The review begins by outlining the sources and types of MPs, emphasizing their widespread presence in marine, freshwater, and terrestrial ecosystems. It then discusses the formation and characteristics of microbial biofilms on MPs surfaces, highlighting their role in altering the physicochemical properties of MPs and facilitating processes such as vertical transport, biodegradation, dispersion of microorganisms, and gene transfer. Different methods used to assess these interactions are discussed, including microbiological and physicochemical characterization. Current gaps and challenges in understanding the complex relationships between biofilms and MPs are identified, highlighting the need for further research to elucidate the mechanisms underlying these complex interactions and to develop effective mitigation strategies. Innovative solutions, including bioremediation techniques and their combination with other strategies, such as nanotechnology, advanced filtration technologies, and public awareness campaigns, are proposed as promising approaches to address the issue of MPs pollution. Overall, this review underscores the urgent need for a multidisciplinary approach to combating MPs pollution, combining scientific research, technological innovation, and public engagement to safeguard the health and integrity of natural ecosystems.
Subject(s)
Biodegradation, Environmental , Biofilms , Ecosystem , Microplastics , Biofilms/growth & development , Bacteria/metabolism , Bacteria/genetics , Plastics/chemistry , Environmental Pollution , Water Pollutants, Chemical/metabolism , Fresh Water/microbiologyABSTRACT
Identifying contaminants of specific bioactivities from complicated environmental matrices remains costly and time-consuming, as it requires us to not only resolve their structures but also determine their bioactivities. Herein, a novel noncovalent tagging method is integrated in mass spectrometry for identifying unknown contaminants that target dopamine (DA) receptors. Via proteolysis of bovine serum albumin, a stereoselective hexapeptide (ACFAVE) is selected for noncovalently tagging the contaminants that possess the stereostructural characteristics of binding to DA receptors. The tagged contaminants can be readily distinguished from the coexisting species for subsequent structural analysis based on the tagging-induced shifts of the mass-to-charge ratios. Thus, both bioactivity evaluation and structure analysis are accomplished via mass spectrometry. By using this method, 1,3-diphenylguanidine (DPG), a widely used additive in rubber and plastics, is successfully identified out of 2495 features detected in the Pearl River water, with its concentration determined as only 9.8 µg L-1. Furthermore, DPG is confirmed as a potential disrupter to the DA receptors via a simulated docking experiment, which has not been reported before. The present noncovalent tagging method provides a cost-effective and time-efficient way of identifying bioactive molecules in complicated matrices. And proteolysis of proteins is promising for developing more taggants with other desired stereoselectivities in the future.
Subject(s)
Water Pollutants, Chemical , Water , Mass Spectrometry , Fresh Water , Plastics , Serum Albumin, Bovine , Water Pollutants, Chemical/analysisABSTRACT
Studies on the adverse effects of nanoplastics (NPs, particle diameter <1000 nm) including physical damage, oxidative stress, impaired cell signaling, altered metabolism, developmental defects, and possible genetic damage have intensified in recent years. However, the analytical detection of NPs is still a bottleneck. To overcome this bottleneck and obtain a reliable and quantitative distribution analysis in complex freshwater ecosystems, an easily applicable NP tracer to simulate their fate and behavior is needed. Here, size- and surface charge-tunable core-shell Au@Nanoplastics (Au@NPs) were synthesized to study the environmental fate of NPs in an artificial freshwater system. The Au core enables the quantitative detection of NPs, while the polystyrene shell exhibits NP properties. The Au@NPs showed excellent resistance to environmental factors (e.g., 1% hydrogen peroxide solution, simulating gastric fluid, acids, and alkalis) and high recovery rates (>80%) from seawater, lake water, sewage, waste sludge, soil, and sediment. Both positively and negatively charged NPs significantly inhibited the growth of duckweed (Lemna minor L.) but had little effect on the growth of cyanobacteria (Microcystis aeruginosa). In addition, the accumulation of positively and negatively charged NPs in cyanobacteria occurred in a concentration-dependent manner, with positively charged NPs more easily taken up by cyanobacteria. In contrast, negatively charged NPs were more readily internalized in duckweed. This study developed a model using a core-shell Au@NP tracer to study the environmental fate and behavior of NPs in various complex environmental systems.
Subject(s)
Cyanobacteria , Microplastics , Bioaccumulation , Ecosystem , Fresh Water , Seawater , PolystyrenesABSTRACT
The genus Odontobutis is a group of freshwater fishes endemic to East Asia. Phylogenetic relationships among the Odontobutis species have never been fully tested due to incomplete taxon sampling and that molecular data have not been collected in many Odontobutis species. In the present study, we sampled 51 specimens from all known eight Odontobutis species with two outgroups (Perccottus glenii and Neodontobutis hainanensis). We collected sequence data of 4434 single-copy nuclear coding loci using gene capture and Illumina sequencing. A robust phylogeny of the Odontobutis with many individuals for each species was built, supporting the current taxonomy that all extant Odontobutis species are valid. The two species from Japan (O. hikimius + O. obscurus) formed an independent clade sister to the "continental odontobutids", whereas the species from southern China (O. sinensis + O. haifengensis) separated from the rest species of the genus. Surprisingly species from the lower reaches of the Yangtze River (O. potamophilus) was more closely related to species from the Korean Peninsula and northeastern China than to the middle reaches of the Yangtze River, such that their relationship was ((O. sinensis + O. haifengensis)(O. platycephala + (O. yaluensis + (O. potamophilus + O. interruptus)))). Divergence time among the Odontobutis was estimated using 100 most clock-like loci and three fossil calibration points. The crown group of the Odontobutis was estimated at 9.0 Ma during the late Miocene (5.6-12.7 Ma, 95% HPDs). Ancestral range of the genus was reconstructed using Reconstruct Ancestral States in Phylogenies (RASP) and BioGeoBEARS. The result suggested that the common ancestor of modern Odontobutis probably was distributed in Japan, southern China or the Korean Peninsula. A series of geographical events in East Asia since the late Miocene, such as the opening of the Japan/East Sea, rapid uplift of the Tibetan Plateau and climate change in the northern region of the Yellow River might account for diversification and current distribution pattern of the Odontobutis.
Subject(s)
Perciformes , Animals , Phylogeny , Sequence Analysis, DNA , Asia, Eastern , Fresh Water , PhylogeographyABSTRACT
Bonytongues (Osteoglossomorpha) constitute an ancient clade of teleost fishes distributed in freshwater habitats throughout the world. The group includes well-known species such as arowanas, featherbacks, pirarucus, and the weakly electric fishes in the family Mormyridae. Their disjunct distribution, extreme morphologies, and electrolocating capabilities (Gymnarchidae and Mormyridae) have attracted much scientific interest, but a comprehensive phylogenetic framework for comparative analysis is missing, especially for the species-rich family Mormyridae. Of particular interest are disparate craniofacial morphologies among mormyrids which might constitute an exceptional model system to study convergent evolution. We present a phylogenomic analysis based on 546 exons of 179 species (out of 260), 28 out of 29 genera, and all six families of extant bonytongues. Based on a recent reassessment of the fossil record of osteoglossomorphs, we inferred dates of divergence among transcontinental clades and the major groups. The estimated ages of divergence among extant taxa (e.g., Osteoglossomorpha, Osteoglossiformes, and Mormyroidea) are older than previous reports, but most of the divergence dates obtained for clades on separate continents are too young to be explained by simple vicariance hypotheses. Biogeographic analysis of mormyrids indicates that their high species diversity in the Congo Basin is a consequence of range reductions of previously widespread ancestors and that the highest diversity of craniofacial morphologies among mormyrids originated in this basin. Special emphasis on a taxon-rich representation for mormyrids revealed pervasive misalignment between our phylogenomic results and mormyrid taxonomy due to repeated instances of convergence for extreme craniofacial morphologies. Estimation of ancestral phenotypes revealed contingent evolution of snout elongation and unique projections from the lower jaw to form the distinctive Schnauzenorgan. Synthesis of comparative analyses suggests that the remarkable craniofacial morphologies of mormyrids evolved convergently due to niche partitioning, likely enabled by interactions between their exclusive morphological and electrosensory adaptations. [Africa; ancestral state estimation; diversity; exon capture; freshwater fishes; Phylogenomics.].
Subject(s)
Electric Fish , Animals , Electric Fish/genetics , Fishes/genetics , Fossils , Fresh Water , Phylogeny , TongueABSTRACT
Protective responses are pivotal in aiding organismal persistence in complex, multi-stressor environments. Multiple-stressor research has traditionally focused on the deleterious effects of exposure to concurrent stressors. However, encountering one stressor can sometimes confer heightened tolerance to a second stressor, a phenomenon termed 'cross-protection'. Cross-protection has been documented in a wide diversity of taxa (spanning the bacteria, fungi, plant and animal kingdoms) and habitats (intertidal, freshwater, rainforests and polar zones) in response to many stressors (e.g. hypoxia, predation, desiccation, pathogens, crowding, salinity, food limitation). Remarkably, cross-protection benefits have also been shown among emerging, anthropogenic stressors, such as heatwaves and microplastics. In this Commentary, we discuss the mechanistic basis and adaptive significance of cross-protection, and put forth the idea that cross-protection will act as a 'pre-adaptation' to a changing world. We highlight the critical role that experimental biology has played in disentangling stressor interactions and provide advice for enhancing the ecological realism of laboratory studies. Moving forward, research will benefit from a greater focus on quantifying the longevity of cross-protection responses and the costs associated with this protective response. This approach will enable us to make robust predictions of species' responses to complex environments, without making the erroneous assumption that all stress is deleterious.
Subject(s)
Adaptation, Physiological , Plastics , Animals , Ecosystem , Acclimatization , Fresh WaterABSTRACT
Packaging ensures the safe handling and distribution of fresh and processed food products via diverse supply chains, and has become an indispensable component of the food industry. However, the rapidly expanding use of plastics, especially single-use plastics, as packaging material leads to inadequate waste management, littering, and consequently serious environmental damage, which predominantly affects marine and freshwater sources. Thus, the use of plastics for packaging purposes has become a major public concern and hence a concern among global policymakers. Notably, 26% of the total volume of global plastic production is primarily used for packaging, of which single-use plastics account for 50%, resulting in pollution that may last hundreds of years. This review provides an overview of the manner in which molded pulp products can be utilized to improve sustainability of food packaging applications, by highlighting the manufacturing processes, signifying characteristics features of recyclable molded pulp, and coupling circularity with eco-friendly and safe food product packaging. In this regard, current concepts advocate the implementation of a dynamic and sustainable approach using molded pulp products. This approach encompasses the design and production of eco-friendly packaging, distribution and consumption of packaged products, and collection and recycling of used packaging for subsequent reuse.
Subject(s)
Plastics , Recycling , Food Packaging , Food , Fresh WaterABSTRACT
Sea ice can serve as a temporary sink for microplastics (MPs), and thus, it too can function as a secondary source of and transport medium for MPs. This study aimed to explore the effect of various MP properties and environmental characteristics on the entrainment and enrichment of MPs in ice under varying turbulence conditions. It was found that high rotation speed in freshwater distinctively enhanced the entrainment of hydrophobic MPs in ice, this being attributable to the combined effects of frazil ice and air bubbles. The hydrophobic nature of these MPs caused them to be attracted to the water/air or water/ice interface. However, in saline water, high turbulence inhibited the entrainment of all of the MP types under study. The ice crystals formed a loose structure in saline water instead of congealing, and this allowed the exchange of MPs between ice and water, leading to the rapid expulsion of MPs from the ice. The enrichment factors of all the MPs under study increased in calm saline water compared to in calm freshwater. The results revealed that the entrainment and enrichment of MPs in ice can be critical pathways affecting their fate in cold regions.
Subject(s)
Microplastics , Water Pollutants, Chemical , Plastics , Water Pollutants, Chemical/analysis , Environmental Monitoring , Fresh WaterABSTRACT
While microplastic transport, fate, and effects have been a focus of studies globally, the consequences of their presence on ecosystem functioning have not received the same attention. With increasing evidence of the accumulation of microplastics at sediment-water interfaces there is a need to assess their impacts on ecosystem engineers, also known as bioturbators, which have direct and indirect effects on ecosystem health. This study investigated the impact of microplastics on the bioturbator Tubifex tubifex alongside any effects on the biogeochemical processes at the sediment-water interface. Bioturbators were exposed to four sediment microplastic concentrations: 0, 700, 7000, and 70000 particles kg-1 sediment dry weight. Though no mortality was present, a significant response to oxidative stress was detected in tubificid worms after exposure to medium microplastic concentration (7000 particles kg-1 sediment dry weight). This was accompanied by a reduction in worm bioturbation activities assessed by their ability to rework sediment and to stimulate exchange water fluxes at the sediment-water interface. Consequently, the contributions of tubificid worms on organic matter mineralization and nutrient fluxes were significantly reduced in the presence of microplastics. This study demonstrated that environmentally realistic microplastic concentrations had an impact on biogeochemical processes at the sediment-water interface by reducing the bioturbation activities of tubificid worms.
Subject(s)
Microplastics , Water Pollutants, Chemical , Plastics , Ecosystem , Geologic Sediments , Water Pollutants, Chemical/analysis , Fresh Water , Water , Environmental MonitoringABSTRACT
Cationic polymer (CP) ecotoxicity is important to understand and investigate as they are widely used in industrial and consumer applications and have shown toxic effects in some aquatic organisms. CPs are identified as "polymers of concern" and are to be prioritized in upcoming regulatory reviews, (e.g., REACH). Algae have generally been found to be the most sensitive trophic level to CP. This study aimed at elucidating the magnitude of cationic polyquaternium toxicity towards algae and to understand key toxicological drivers. A suite of polyquaterniums with varying charge density (charged nitrogen moieties) and molecular weight were selected. Highly charged polyquaternium-6 and -16 were toxic towards the freshwater green microalgae Raphidocelis subcapitata with ErC50-values ranging between 0.12 and 0.41 mg/L. Lower charge density polyquaternium-10 materials had much lower toxicity with ErC50 > 200 mg/L, suggesting that charge density is an important driver of algal toxicity. These levels of toxicity were in line with historic CP data in literature. Algal agglomeration was observed in all tests but was not linked with impacts on algal growth rate. However, agglomeration can pose challenges in the technical conduct of tests and can impair interpretation of results. The toxicity mitigation potential of humic acid was also explored. The addition of 2-20 mg/L humic acid completely mitigated PQ6 and PQ16 toxicity at concentrations higher than clean water ErC50-values. CP toxicity mitigation has also been observed in fish and invertebrate tests, suggesting that CP mitigation should be accounted for in all trophic levels within an environmental safety framework.