Factors impacting the interactions of engineered nanoparticles with bacterial cells and biofilms: Mechanistic insights and state of knowledge.

Since their advent a few decades ago, engineered nanoparticles (ENPs) have been extensively used in consumer products and industrial applications and their use is expected to continue at the rate of thousands of tons per year in the next decade. The widespread use of ENPs poses a potential risk of large scale environmental proliferation of ENPs which can impact and endanger environmental health and safety. Recent studies have shown that microbial biofilms can serve as an important biotic component for partitioning and perhaps storage of ENPs released into aqueous systems. Considering that biofilms can be one of the major sinks for ENPs in the environment, and that the field of biofilms itself is only three to four decades old, there is a recent and growing body of literature investigating the ENP-biofilm interactions. While looking at biofilms, it is imperative to consider the interactions of ENPs with the planktonic microbial cells inhabiting the bulk systems in the vicinity of surface-attached biofilms. In this review article, we attempt to establish the state of current knowledge regarding the interactions of ENPs with bacterial cells and biofilms, identifying key governing factors and interaction mechanisms, as well as prominent knowledge gaps. Since the context of ENP-biofilm interactions can be multifarious-ranging from ecological systems to water and wastewater treatment to dental/medically relevant biofilms- and includes devising novel strategies for biofilm control, we believe this review will serve an interdisciplinary audience. Finally, the article also touches upon the future directions that the research in the ENP-microbial cells/biofilm interactions could take. Continued research in this area is important to not only enhance our scientific knowledge and arsenal for biofilm control, but to also support environmental health while reaping the benefits of the 'nanomaterial revolution'.

Review of chemical pretreatment of lignocellulosic biomass using low-liquid and low-chemical catalysts for effective bioconversion.

Chemical pretreatment of lignocellulosic biomass (LCB) is essential for effective biological conversion in subsequent steps to produce biofuels or biochemicals. For effective pretreatment, high lignin content and its recalcitrant nature of LCB are major factors influencing bioconversion, especially lignin is known to be effectively solubilized by alkaline, organic, and deep eutectic solvents, ionic liquids, while hemicellulose is effectively dissolved by various acid catalysts and organic solvents. Depending on the pretreatment method/catalyst used, different pretreatment process scheme should be applied with different amounts of catalyst and water inputs to achieve a satisfactory effect. In addition, the amount of processing water required in the following processes such as washing, catalyst recovery, and conditioning after pretreatment is critical factor for scale-up (commercialization). In this review, the amount of catalyst and/or water used, and the effect of pretreatment, properties of the products, and recovery of liquid are also discussed.

Rapid immobilization of viable Bacillus pseudomycoides in polyvinyl alcohol/glutaraldehyde hydrogel for biological treatment of municipal wastewater.

A new approach for easy synthesis of Bacillus pseudomycoides immobilized polyvinyl alcohol (PVA)/glutaraldehyde (GA) hydrogel for application in a wastewater treatment system is reported. Optimization studies revealed that GA/PVA mass ratio of 0.03 and acidic pH of 2 were required for hydrogel synthesis and eventually for bacterial cell immobilization. The synthesized crosslinked matrix possessed a pore size suitable for microbial cell entrapment while maintaining cell accessibility to external environment for bioremediation. Possible crosslinking and bacterial cell immobilization in the hydrogel were evidenced by FTIR, XRD, and SEM studies, respectively. Further, the extent of crosslinking of GA with PVA was investigated and confirmed by transmittance and permeability experiments. The viability and proliferation of hydrogel embedded cells (after 25 days) was confirmed by confocal fluorescence microscopy which also indicated that acidic pH of polymer solution did not affect the immobilized live cells. B. pseudomycoides immobilized hydrogel were demonstrated to be effective for treatment of municipal wastewater and reduced biochemical oxygen demand (BOD), chemical oxygen demand (COD), and protein content below the recommended levels. Overall, the results from this bench-scale work show that employing bacteria-embedded PVA/GA hydrogel for the treatment of municipal wastewater yield promising results which should be further explored in pilot/field-scale studies.