RESUMO
A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.
Assuntos
Núcleo Celular/química , DNA Cruciforme/química , DNA de Cadeia Simples/química , Géis/química , Bicamadas Lipídicas/química , Modelos Biológicos , Núcleo Celular/genética , Núcleo Celular/metabolismo , DNA Cruciforme/genética , DNA Cruciforme/metabolismo , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/metabolismo , Dimetilpolisiloxanos/química , Escherichia coli/genética , Géis/metabolismo , Redes Reguladoras de Genes , Proteínas de Fluorescência Verde , Interações Hidrofóbicas e Hidrofílicas , Bicamadas Lipídicas/metabolismo , Conformação de Ácido Nucleico , Plasmídeos , Biossíntese de ProteínasRESUMO
Liposomes are widely used in industrial engineering systems including cosmetics and pharmaceutical and biotechnological applications. A fundamental understanding of the dynamics of lipid bud formation is required to efficiently produce various high-technological liposomes with well-controlled sizes and shapes. In this study, a novel patterning method of controlling the uniformity of the sizes and shapes of liposome buds nucleated from a flat lipid film casted on a flat substrate is reported. The dried-out lipid components had been swelling during aqueous hydration, and they pinched away in approximately one hour. The sizes and spacing of the liposome buds were monitored during hydration. It was observed that the average size of the buds slowly increased, but their spacing did not significantly change. Moreover, the bud size distribution was a very narrow Gaussian, which implies the formation of buds with uniform sizes. The analytical calculation of the equilibrium state was theoretically developed and compared with the experiments. It is envisioned that this study will provide insights on a sustained-release drug vehicle.
Assuntos
Lipossomos/química , Lipossomos/metabolismo , Lipídeos de Membrana/química , Modelos Químicos , Biotecnologia , Cinética , Nanotecnologia , Tamanho da Partícula , Fosfatidilcolinas/química , Água/químicaRESUMO
A facile deposition process was developed for the fabrication of a new superhydrophobic layer composed of an underlying zinc oxide nanoparticle support and a gold top layer doped with the hydrophobic chemical, heptadecafluoro-1-decanethiol (HDFT). The resulting microscaled and spherical DNA-based hydrogels could serve as a platform for pseudo-nucleus mimics.