RESUMO
"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.
Assuntos
Quelantes , DNA Bacteriano/isolamento & purificação , Bactérias Gram-Negativas/genética , Bactérias Gram-Positivas/genética , Reação em Cadeia da Polimerase/métodos , Amplificação de Genes , Técnicas In Vitro , Resinas Sintéticas , Streptococcus agalactiae/genéticaRESUMO
During a 10-week period, 16 patients in a neurosurgery intensive care unit were involved in an outbreak of Serratia marcescens. The epidemic strain was found in several flasks of 1:4 diluted hexetidine solution, an antiseptic used for patient mouth washing. Testing of the bactericidal activity of the diluted antiseptic revealed that all the epidemic strains were able to grow in the diluted antiseptic solution. Strains isolated from clinical samples and from the antiseptic solution were compared by random amplification of polymorphic DNA. Epidemiologic typing data implicated the diluted antiseptic solution as the single source of this S. marcescens outbreak.
Assuntos
Anti-Infecciosos Locais , Infecção Hospitalar/etiologia , Surtos de Doenças , Contaminação de Medicamentos , Hexitidina , Infecções por Serratia/etiologia , Serratia marcescens , Humanos , Controle de Infecções , Testes de Sensibilidade Microbiana , Antissépticos Bucais , Técnica de Amplificação ao Acaso de DNA Polimórfico , SoluçõesRESUMO
We propose an one-step DNA extraction method suitable for the polymerase chain reaction. This procedure utilizes Chelex 100, a chelating in exchange resin. This technique was compared with a traditional technique (proteinase K lysis, phenol-chloroform extraction and ethanol precipitation) for isolation of human cytomegalovirus DNA from clinical samples. The procedure using Chelex 100 appeared to be a simple and fast extraction method for human cytomegalovirus DNA.
Assuntos
Infecções por Citomegalovirus/diagnóstico , DNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase , Líquido da Lavagem Broncoalveolar/microbiologia , Humanos , Leucócitos/microbiologia , Saliva/microbiologia , Urina/microbiologiaRESUMO
The authors report a case of pseudo-tumoral thoracic actinomycosis with lysis of a rib in a young man who was a heavy smoker and drinker. This observation is an opportunity to review the usual difficulties of diagnosis in patients with this rare disease occurring most often in a patient who is debilitated with important disease in the teeth and gingival margins. The standard treatment is penicillin G, which leads to a cure within one month.