Uncommon true isolated macrodontia of a maxillary tooth.

Macrodontia is a rare dental anomaly which consists on the presence of any tooth or teeth larger than the average. In the present report the case of a child with an uncommon true isolated macrodontia of a maxillary central incisor is presented. The maxillary left central incisor was considered a macrodontic tooth since its mesiodistal crown dimension was 12.41 mm while the mesiodistal crown dimension of the maxillary right central incisor was 10.06 mm. The radiographic examination confirmed an enlarged maxillary left central incisor and a normal developing dentition. The patient did not report esthetic complaints, however, due the presence of anterior open bite and crowding, he was referred to orthodontic clinic. Thus, it must be point out the importance of the radiographic diagnosis to support the clinical findings, helping to plan and provide better treatment for the patient.

Eficácia da intervenção educacional à distância na capacitação de cirurgiões-dentistas da rede pública sobre o uso consciente da tomografia computadorizada de feixe cônico: um ensaio clínico controlado quase-experimental / Efficacy of distance educational intervention in training public dentists on the conscious use of cone beam computed tomography: a quasi-experimental controlled clinical trial

Objetivo: Verificar a eficácia da intervenção educacional por videoconferência, para dentistas de um serviço público de saúde, sobre aquisição de conhecimentos em tomografia computadorizada de feixe cônico. Materiais e Métodos: Trata-se de um ensaio clínico controlado, com aplicação de questionários estruturados sobre uso criterioso da tomografia computadorizada de feixe cônico, antes e depois da intervenção educacional. Os participantes do estudo foram divididos em grupos controle (aula presencial) e experimental (aula por videoconferência). Participaram desta pesquisa 133 dentistas no pré-teste (questionário aplicado antes da intervenção) e 129 no pós-teste (questionário aplicado após a intervenção). As análises foram executadas para avaliar as variáveis da metodologia de ensino e comparar a proporção das respostas corretas e "não sei" entre os tempos, discriminadas pela especialidade odontológica dos participantes. O nível de significância adotado foi de 0,05. Foram utilizados os testes de qui-quadrado e o modelo de regressão logística binária e, quando significativo, foi aplicado o teste post-hoc de Bonferroni. Resultados: A análise das respostas "não sei" e respostas corretas entre as modalidades de ensino presencial e por videoconferência demonstrou que não houve mudança significativa dos resultados. O resultado das respostas "não sei" evidenciou uma grande diferença entre os tempos, onde o percentual dessas respostas, após a intervenção, variou de 23,6% para 4,7% (p<0,001). Em todas as especialidades, essa redução foi significativa nas questões referentes ao conhecimento sobre radioproteção. O aumento de respostas corretas foi de quase 10% (p<0,001). Conclusão: A capacitação profissional através do método de videoconferência mostrou-se eficaz e comparável ao método presencial para a aquisição de conhecimentos em tomografia computadorizada de feixe cônico.

Objective: To verify the effectiveness of an educational intervention by videoconference, for dentists of a public health service, on acquiring knowledge in cone-beam computed tomography. Materials and Methods: This is a controlled clinical trial with structured questionnaires on the judicious use of cone-beam computed tomography, before and after the educational intervention. Study participants were divided into control (faceto-face class) and experimental (videoconference class) groups. A total of 133 dentists participated in the pre-test (questionnaire applied before the intervention) and 129 in the post-test (questionnaire applied after the intervention). The analyzes were performed to evaluate the variables of the teaching methodology and to compare the proportion of correct and "I do not know" answers between times, broken down by the dental specialty of the participants. The level of significance adopted was 0.05. The chi-square tests and the binary logistic regression model were used and, when significant, the Bonferroni post-hoc test was applied. Results: The analysis of the answers "I don't know" and correct answers between the teaching modalities showed no significant change in the results. The result of the "I don't know" answers showed a significant difference between the times, where the percentage of these answers, after the intervention, varied from 23.6% to 4.7% (p<0.001). In all specialties, this reduction was significant in questions related to knowledge about radioprotection. The increase incorrect answers were almost 10% (p<0.001). Conclusion: Professional training through the videoconference method proved effective and comparable to the face-to-face method for acquiring knowledge in cone-beam computed tomography.

Myeloid sarcoma of the oral cavity: A case report and review of 89 cases from the literature.

Myeloid sarcoma is a tumor mass of immature myeloid or granulocytic cells that affects extramedullary anatomic sites, including uncommonly the oral cavity. A 24-year-old female was referred for evaluation of a fast growing painful gingival swelling lasting 2 weeks, associated with fever, fatigue, and cervical lymphadenopathy. Intraoral examination showed a bluish swelling on the right posterior lower gingiva exhibiting necrotic surface. Incisional biopsy of the gingival lesion displayed diffuse infiltration of undifferentiated tumor cells with granulocytic appearance, strongly immunopositive for CD99, myeloperoxidase and Ki-67 (60%), and negative for CD20, CD3, CD34 and TdT. Blood tests presented a severe pancytopenia, and genetic analysis confirmed the diagnosis of acute promyelocytic leukemia. The final diagnosis was of oral myeloid sarcoma associated with acute promyelocytic leukemia with t(15;17). The patient was submitted to chemotherapy but died of the disease one month later. The clinicopathologic and immunohistochemical features of the present case are compared with the 89 cases of oral myeloid sarcoma previously reported in the English-language literature. Key words:Myeloid sarcoma, chloroma, granulocytic sarcoma, gingiva, oral, acute promyelocytic leukemia, acute myeloid leukemia.

Are salivary flow rates associated with histopathologic aspects in patients with rheumatoid arthritis?

Abstract The aim of this retrospective cross-sectional study was to verify the association between salivary flow rates (SFR) and the histopathologic aspects of labial salivary glands (LSG) in patients with rheumatoid arthritis (RA). Patients presenting rheumatologic diseases referred for oral evaluation were included in the study if they had RA and had SFR measured and LSG biopsy performed. Patients were excluded if they had systemic conditions that affect SFR or if they were being treated for hyposalivation. Cases without enough material for histopathologic analysis were also excluded. Data were collected through questionnaires, oral examination, resting and stimulated SFR, and LSG biopsies. A histopathologic reevaluation was carried out in order to seek for additional histopathologic aspects. Fifty-one patients met the inclusion criteria. The mean age was 53.5 years (25-77), and 94.1% were women. The median resting and stimulated SFRs were 0.24 mL/min and 1.02 mL/min, respectively. The presence of lymphocytic focus and fibrosis were significantly associated with stimulated SFR, but not with resting SFR. The odds ratio of patients who had hyposalivation for presenting a positive lymphocytic focus was 7.33 (confidence interval CI: 1.53-35.23) by the stimulated technique, and 2.56 (CI: 0.57-11.40) in resting SFR. In the medical records, 14 (31.80%) patients had been diagnosed with secondary Sjögren's syndrome. In conclusion, stimulated SFR represent a good screening test to predict lymphocytic focus in LSG in patients with RA, which represents the most specific test to diagnose Sjögren's syndrome.

Oral lichen planus part I: epidemiology, clinics, etiology, immunopathogeny, and diagnosis.

This article presents a review of the literature on oral lichen planus, focusing on important aspects of its epidemiology, etiopathogeny, and clinical manifestations. The oral form is an important clinical presentation of lichen planus. It may precede or accompany the skin lesions, or it may be the only manifestation of the disease. Dermatologists, dentists, otorhinolaryngologists, and other specialists who deal with the oral cavity must be aware of this disease so they can diagnose, treat, and keep these patients under observation.

Clinicopathologic analysis and syndecan-1 and Ki-67 expression in calcifying cystic odontogenic tumors, dentinogenic ghost cell tumor, and ghost cell odontogenic carcinoma.

OBJECTIVE: Benign and malignant tumor cells can express altered adhesion properties, and these features can be associated with their proliferative and invasive characteristics. This study aimed to evaluate syndecan-1 and Ki-67 expression in ghost cell-containing odontogenic tumors. STUDY DESIGN: Clinical data were retrieved from laboratory records, and hematoxylin-eosin-stained slides and sections, labeled with monoclonal antibodies anti-syndecan-1 and anti-Ki-67 using the immunoperoxidase technique, were evaluated. RESULTS: Included were 21 central calcifying cystic odontogenic tumors (CCOTs) (4 associated with odontoma), 2 peripheral CCOTs, 1 dentinogenic ghost cell tumor, and 1 ghost cell odontogenic carcinoma (GCOC). Syndecan-1 was mainly expressed in cells resembling stellate reticulum and in stromal cells from the fibrous capsule. The mean Ki-67 labeling index was 4.1% (49.3% for GCOC), but it was not associated with syndecan-1 expression. CONCLUSIONS: Syndecan-1 is variably expressed in cells resembling the stellate reticulum, stromal cells, and basal cells and might be associated with the biology of these tumors.

Tumor-infiltrating macrophage and microvessel density in oral squamous cell carcinoma.

Tumor-associated macrophages (TAM) are the main cellular component in stroma of many tumors and participate in tumor angiogenesis. The aim of present study was to compare the microvascular density (MVD) and infiltrating macrophage density (IMD) in oral squamous cell carcinomas (OSCCs) with different histological grades. A histomorphometric analysis was performed after immunohistochemistry using antibodies such as von-Willebrand factor and CD68. A significant difference in MVD was found between well and moderately differentiated OSCCs (p<0.05). TAM were largely present in all studied tumors and the IMD was not different among OSCCs with different histological grades (p=0.381). Significant correlation between MVD and IMD was not observed (p=0.870). In conclusion, these results suggest that TAM and angiogenesis have an influence at different histological grades of OSCC. However, the lack of correlation between MVD and IMD could suggest that angiogenesis does not depend on the number of macrophages present in OSCC, but their predominant phenotype. Further studies involving distinct phenotypes of macrophages should be done to better understand the influence of TAM on the tumor angiogenesis.

Calcifying epithelial odontogenic tumor (CEOT): a clinicopathologic and immunohistochemical study and comparison with dental follicles containing CEOT-like areas.

OBJECTIVE: To describe the clinicopathologic, immunohistochemical, and scanning electron microscopic features of 19 cases of calcifying epithelial odontogenic tumor (CEOT) in comparison to 4 cases of dental follicles containing CEOT-like areas (DF-CEOT). STUDY DESIGN: A collaborative Latin American retrospective study. RESULTS: CEOT and DF-CEOT showed a slight predilection for females, mostly affecting the posterior mandible. CEOTs were classified as epithelium-rich (8 cases), amyloid-rich (4), and calcification-rich (3), and 4 cases showed similar proportion of the 3 components. DF-CEOTs contained odontogenic epithelium, amyloid, calcification, and clear cells. Epithelial cells were positive for cytokeratins CK5 and CK19, E-cadherin, and syndecan 1 (CD138), and focally for amyloid A. In CEOT, amyloid was positive for CD138 and amyloid A, and calcification for CK5, CD138, and amyloid A. In DF-CEOT, calcification was positive for amyloid A. CEOT showed higher Ki-67 protein and minichromosome maintenance complex component 2 (MCM-2) labeling indices than did DF-CEOT. In scanning electron microscopy, CEOT calcified material resembled bone in the 3 cases classified as calcification-rich. CONCLUSIONS: CEOT and DF-CEOT showed histomorphologic and immunohistochemical similarities, and the histogenetic significance of these features should be further studied.

Histopathological features of keratocystic odontogenic tumor: a descriptive study of 177 cases from a Brazilian population.

The aim of this study was to describe the clinicopathologic features of 177 keratocystic odontogenic tumors (KCOTs) diagnosed in a Brazilian population. A total of 177 KCOTs were reviewed and affected 158 patients with ages ranging from 5 to 79 years (mean age = 32 years) with a slight female predominance. Mandible was the most common affected site (69.3%), and a unilocular radiolucency was the most common radiographic image. Microscopically, all cases showed at least focal areas of classic KCOT, but several histological aspects were also observed, including diffuse and focal epithelial lining hyperplasia (48.6%), epithelial budding (12.4%), reactive cytological alterations (11.3%), dystrophic calcification (7.9%), daughter cysts (7.8%), odontogenic epithelial remnants (4.5%), focal areas of orthokeratinization (2.8%), and ameloblastomatous epithelium (1.7%). These variations may make KCOT diagnosis challenging in some cases, so careful full-sample analysis and knowledge of these uncommon histological features associated with KCOT are essential for correct diagnosis.

Biocompatibility of orthodontic adhesives in rat subcutaneous tissue.

OBJECTIVE: The objective of the present study was to verify the hypothesis that no difference in biocompatibility exists between different orthodontic adhesives. MATERIAL AND METHODS: Thirty male Wistar rats were used in this study and divided into five groups (n=6): Group 1 (control, distilled water), Group 2 (Concise), Group 3 (Xeno III), Group 4 (Transbond XT), and Group 5 (Transbond plus Self-Etching Primer). Two cavities were performed in the subcutaneous dorsum of each animal to place a polyvinyl sponge soaked with 2 drops of the respective adhesive in each surgical loci. Two animals of each group were sacrificed after 7, 15, and 30 days, and their tissues were analyzed by using an optical microscope. RESULTS: At day 7, Groups 3 (Transbond XT) and 4 (Xeno III) showed intense mono- and polymorphonuclear inflammatory infiltrate with no differences between them, whereas Groups 1 (control) and 2 (Concise) showed moderate mononuclear inflammatory infiltrate. At day 15, severe inflammation was observed in Group 3 (Transbond XT) compared to other groups. At day 30, the same group showed a more expressive mononuclear inflammatory infiltrate compared to other groups. CONCLUSION: Among the orthodontic adhesive analyzed, it may be concluded that Transbond XT exhibited the worst biocompatibility. However, one cannot interpret the specificity of the data generated in vivo animal models as a human response.

Recurrent bilateral gingival peripheral calcifying epithelial odontogenic tumor (Pindborg tumor): a case report.

Calcifying epithelial odontogenic tumor (CEOT) is an extremely rare, benign neoplasm, accounting for approximately 1% of all odontogenic tumors. Peripheral CEOTs commonly resemble oral hyperplastic or reactive lesions and are histologically similar to their intraosseous counterparts. We report an unusual case of multifocal peripheral CEOT. A 40-year-old female presented with bilateral soft, painful, erythematous, gingival swellings localized in premolar areas of the mandibular gingiva. The presumptive diagnosis was bilateral pyogenic granuloma. The masses were surgically excised under local anesthesia without bone curettage and both recurred 12 months later. Morphologic features, and histochemical and immunohistochemical tests revealed bilateral peripheral calcifying odontogenic epithelial tumor. There is no clinical or radiographic evidence of recurrence 3.5 years after excision. This multifocal phenomenon has been reported previously only for intraosseous CEOT. Gingival masses must be carefully evaluated for clinical and histologic evidence of neoplasia.

Tumor-Infiltrating Macrophage and Microvessel Density in Oral Squamous Cell Carcinoma

Tumor-associated macrophages (TAM) are the main cellular component in stroma of many tumors and participate in tumor angiogenesis. The aim of present study was to compare the microvascular density (MVD) and infiltrating macrophage density (IMD) in oral squamous cell carcinomas (OSCCs) with different histological grades. A histomorphometric analysis was performed after immunohistochemistry using antibodies such as von-Willebrand factor and CD68. A significant difference in MVD was found between well and moderately differentiated OSCCs (p<0.05). TAM were largely present in all studied tumors and the IMD was not different among OSCCs with different histological grades (p=0.381). Significant correlation between MVD and IMD was not observed (p=0.870). In conclusion, these results suggest that TAM and angiogenesis have an influence at different histological grades of OSCC. However, the lack of correlation between MVD and IMD could suggest that angiogenesis does not depend on the number of macrophages present in OSCC, but their predominant phenotype. Further studies involving distinct phenotypes of macrophages should be done to better understand the influence of TAM on the tumor angiogenesis.

Macrófagos associados a tumores (MAT) representam o componente principal do estroma de muitos tumores, além de participar da angiogênese tumoral. Este estudo comparou a microdensidade vascular (MDV) e densidade de macrófagos infiltrando o tumor (DMIT) em carcinoma escamocelular da boca (CEC) com diferentes graus histológicos de malignidade. Análise histomorfométrica foi empregada após técnica imuno-histoquímica para os anticorpos fator von-Willebrand e CD68. Uma diferença significante entre MDV e carcinomas bem e moderadamente diferenciados foi observada (p<0,05). MAT estavam fortemente presentes em todos os tumores estudados e a DMIT não foi diferente entre os diferentes graus histológicos de malignidade do CEC (p=0,381). Correlação significante entre MDV e DMIT não foi observada (p=0,870). Em conclusão, os resultados desse estudo sugerem a influência de MAT e angiogênese nos diferentes graus histológicos de malignidade do CEC. Entretanto, a ausência de correlação entre MDV e DMIT sugere que a angiogênese não depende do número de macrófagos presentes neste tipo de câncer, mas do fenótipo predominante. Outros estudos devem ser realizados a fim de contribuir para melhor compreensão da participação de MAT na angiogênese tumoral.

Biocompatibility of orthodontic adhesives in rat subcutaneous tissue

ABSTRACT OBJECTIVE: The objective of the present study was to verify the hypothesis that no difference in biocompatibility exists between different orthodontic adhesives. MATERIAL AND METHODS: Thirty male Wistar rats were used in this study and divided into five groups (n=6): Group 1 (control, distilled water), Group 2 (Concise), Group 3 (Xeno III), Group 4 (Transbond XT), and Group 5 (Transbond plus Self-Etching Primer). Two cavities were performed in the subcutaneous dorsum of each animal to place a polyvinyl sponge soaked with 2 drops of the respective adhesive in each surgical loci. Two animals of each group were sacrificed after 7, 15, and 30 days, and their tissues were analyzed by using an optical microscope. RESULTS: At day 7, Groups 3 (Transbond XT) and 4 (Xeno III) showed intense mono- and polymorphonuclear inflammatory infiltrate with no differences between them, whereas Groups 1 (control) and 2 (Concise) showed moderate mononuclear inflammatory infiltrate. At day 15, severe inflammation was observed in Group 3 (Transbond XT) compared to other groups. At day 30, the same group showed a more expressive mononuclear inflammatory infiltrate compared to other groups. CONCLUSION: Among the orthodontic adhesive analyzed, it may be concluded that Transbond XT exhibited the worst biocompatibility. However, one cannot interpret the specificity of the data generated in vivo animal models as a human response.

Síndrome de Sjogren e sialadenites inespecíficas: estudo do infiltrado inflamatório das glândulas salivares / Sjogren's syndrome and unspecific sialadenitis: study of the inflammatory infiltrate of salivary glands

As glândulas salivares säo sítio freqüente de patologias localizadas e sistêmicas, apresentando infiltraçäo de células inflamatórias, acompanhada de alteraçöes morfológicas e funcionais. Utilizando-se da técnica da imuno-histoquímica, foi avaliada a presença e a distribiçäo de células mononucleares, identificadas pelos anticorpos monoclonais CD20, CD45RO, CD8, CD57, CD68 e Plasma Cell, em glândulas salivares menores de lábio inferior de pacientes com síndrome de Sjogren (SS). Os mesmos procedimentos foram realizados para casos de sialadenites inespecíficas näo auto-imunes. As diferenças entre ambas as patologias, para cada um dos anticorpos usados, näo foram estatisticamente significativas. Entretanto, o infiltrado inflamatório observado nas glândulas salivares de pacientes com SS apresentou distribuiçäo focal e tendência a maior concentraçäo de células CD20+, CD45RO+ e CD57+, em comparaçäo ao infiltrado presente nas sialadenites inespecíficas. Estas, por sua vez, mostraram padräo inflamatório predominantemente difuso e tendência a maior concentraçäo de células CD68+, Plasma Cell+, e CD8+ em relaçäo à SS. Os resultados sugerem que, na SS, a resposta imunológica é caracterizada pela organizaçäo de áreas semelhantes a folículos linfóides, para a diferenciaçäo de linfócitos B em células produtoras de imunoglobulinas, com o auxílio de subclasses de linfócitos T. Nas sialadenites inespecíficas, a resposta imunológica é secundária às alteraçöes atróficas locais, tem caráter autolimitante e näo ocasiona maiores prejuízos funcionais

Prevalência de patologias sistêmicas ou condiçöes especiais em pacientes odontológicos atendidos em pronto-socorro: análise de 2.000 atendimentos / The prevalence of the systemic pathologies or special conditions in patients attended at the odontological emergency service: two thousand consultation evaluation

Um total de 2000 pacientes atendidos no Serviço de Emergência Odontológica do Hospital Universitário Antônio Pedro foram submetidos à anamnese padräo, a fim de avaliar a prevalência de patologias sistêmicas ou condiçöes especiais e sua relaçäo com possíveis ligaçöes que eventualmente pudessem ocorrer durante o atendimento. Verificou-se que 10,55 por cento dos pacientes eram medicamente comprometidos, sendo estes, pricipalemnte adultos e do sexo feminino. A hipertensäo arterial foi a alteraçäo mais comumente constatada. Durante os atendimentos ocorreram 25 episódios emergenciais, a maioria, associados ao grupo dito especial

Fatores prognósticos do câncer bucal / Prognostic factors of oral cancer

O carcinoma de células escamosas é o tumor maligno que tem, geralmente o seu prognóstico e tratamento baseados no estadiamento e no grau de diferenciaçäo histológica. Entretanto, como nem sempre a evoluçäo clínica do processo segue os parâmetros clínico-patológicos esperados, novos fatores prognósticos estäo sendo estudados. O objetivo deste trabalho é fazer uma revisão da literatura acerca dos fatores prognósticos do carcinoma de células escamosas bucais e mostrar que é necessário fazer a correlaçäo destes fatores para determinar o comportamento biológico do tumor

Estudo da expressäo imunohistoquímica de células mononucleares CD20+, CD45RO+, CD57+, CD68+, CD8+ e plasma cell+, no infiltrado inflamatório de glândulas salivares menores associadas à síndrome de Sjögren e nas sialadenites inespecíficas / Immunohistochemical study of the presence and distribution of CD20+, CD45RO+, CD8+, CD57+, CD68+ and plasma cell+ inflammatory mononuclear cells, in Sjögren's syndrome associated minor salivary glands, and in inespecific sialadenitis

As glândulas salivares säo sítio freqüente de patologias localizadas e sistêmicas, apresentando infiltraçäo de células inflamatórias, acompanhada de alteraçöes morfológicas e funcionais. Utilizando-se da técnica da imunohistoquímica foi avaliada a presença e a distribuiçäo de células mononucleares, identificadas pelos anticorpos monoclonais CD20, CD45RO, CD8, CD57, CD68 e Plasma Cell, em glândulas salivares menores de lábio inferior de pacientes com Síndrome de Sjögren, uma exocrinopatia de natureza autoimune. Os mesmos procedimentos foram realizados para casos de sialadenites inespecíficas, näo auto imunológicas. Os resultados comparativos entre ambas as patologias, para cada um dos anticorpos usados, näo foram estatisticamente significativos. Entretanto, o infiltrado inflamatório nas glândulas salivares na SS apresentaram distribuiçäo focal e tendência à maior concentraçäo de células CD20+, CD45RO+ e CD57+, em comparaçäo às sialadenites inespecíficas. Estas, por sua vez, mostraram padräo inflamatório predominantemente difuso e tendência à maior concentraçäo de células CD68+, Plasma Cell e CD8, em relaçäo à SS. Os resultados sugerem que na SS, a resposta imunológica é caracterizada pela organizaçäo de áreas semelhantes e folículos linfóides, para a diferenciaçäo de linfócito B em células produtoras de imunoglobulinas, com o auxílio de subclasses de linfócitos T. Nas sialadenites inespecíficas, a resposta imunológica é secundária as alteraçöes atróficas locais, de caráter autolimitante e, sem ocasionar maiores prejuízos funcionais