Aquatic toxicity of chemically defined microplastics can be explained by functional additives.

A novel, systematic approach to relate plastic toxicity with chemical composition is undertaken. Using industrial methods, three petroleum-based polymers, low-density polyethylene (PE), polyvinyl chloride (PVC), and polyamide (PA), and the biopolymer polyhydroxybutyrate (PHB) were manufactured in different formularies including conventional and alternative additives, and microplastics of two sizes (<250 and <20 µm) were obtained with the aim to relate their composition with environmental impact in aquatic environments. Internationally accepted standard tests of regulatory use with marine organisms representative of microalgae (Tisochrysis lutea population growth), crustaceans (Acartia clausi larval survival), and echinoderms (Paracentrotus lividus sea-urchin embryo test) support the following conclusions. Aquatic toxicity of microplastics made from conventional oil-based polymers is due to leaching of chemical additives, and not to ingestion of microplastics. Use of alternative formulations based on natural rather than synthetic chemical additives did not consistently reduce aquatic toxicity except for the replacement of triclosan by the alternative biocide lawsone. In contrast, the biopolymer tested, PHB, seemed to impact marine plankton through different mechanisms associated to the higher abundance of plastic particles within the nanometric range found in this resin and absent in other materials.

Polyethylene microplastics do not increase bioaccumulation or toxicity of nonylphenol and 4-MBC to marine zooplankton.

Global production of synthetic polymers, led by polyethylene (PE), rose steadily in the last decades, and marine ecosystems are considered as a global sink. Although PE is not biodegradable, in coastal areas it fragments into microplastics (MP) readily taken up by biota, and have been postulated as vectors of hydrophobic chemicals to marine organisms. We have tested this hypothesis using two organisms representative of the marine plankton, the holoplanktonic copepod Acartia clausi, and the meroplanktonic larva of the Paracentrotus lividus sea-urchin, and two model chemicals with similar hydrophobic properties, the 4-n-Nonylphenol and the 4-Methylbenzylidene-camphor used as plastic additive and UV filter in cosmetics. Both test species actively ingested the MP particles. However, the presence of MP never increased the bioaccumulation of neither model chemicals, nor their toxicity to the exposed organisms. Bioaccumulation was a linear function of waterborne chemical disregarding the level of MP. Toxicity, assessed by the threshold (EC10) and median (EC50) effect levels, was either independent of the level of MP or even in some instances significantly decreased in the presence of MPs. These consistent results challenge the assumption that MP act as vectors of hydrophobic chemicals to planktonic marine organisms.