A sensitive and reversible staining of proteins on blot membranes.

A modified, sensitive and reversible method for protein staining on nitrocellulose (NC) and polyvinylidine fluoride (PVDF) membranes was developed in Western blotting. The method employed Congo red staining to visualize proteins on different blot membranes. Staining of proteins with Congo red dye is more faster procedures. According to the experimental results, approximate 20 ng proteins could be detected in 3 min in room temperature. The staining on the proteins is easily reversible with Congo red destaining solution for NC and PVDF membranes, so that the blot membranes can be reused for Western blotting. In addition, we confirmed that the staining method is fully compatible with Western blot detection. NC and PVDF membranes treatment with Congo red staining does not interfere with conventional chemiluminescent substrates of peroxidase. As compared to MemCode reversible protein stain kits from Pirece Biotechnology, the staining technique is more sensitive, lower of cost, convenient and not adversely affecting subsequent Western blotting results. On the other hand, the stain is more sensitive than the Ponceau S staining. Therefore, Congo red staining is a promising and ideal alternative for current protein stain. Besides, the binding modes of Congo red or Ponceau S stain were investigated using various 2D and 3D molecular docking and demonstrated potential molecular basis for sensitivity of Congo red staining are higher than Ponceau S.

[Research progress in co-delivery of gene and chemotherapy drugs with cationic liposome carrier for cancer therapy].

Despite recent advances in conventional therapeutic approaches for cancer, the efficacy of chemotherapy for cancer is limited due to the drug resistance and toxic side effects during treatment. To overcome drug resistance, higher doses of the toxic chemotherapy drugs are frequently administered, thus leading to even severe adverse side effects, which have limited their clinical application. Cationic liposome as a novel non-viral carrier for co-delivery of gene and chemotherapy drugs in cancer gene therapy has already attracted more and more attention in recent years. Most importantly, this combined strategy can generate a significant synergistic effect, which can silence the related gene expression and increase the concentration of the intracellular chemotherapy drugs. This approach allows the use of a much lower dose of the chemotherapy drugs to achieve same therapeutic effect, which may have the potential for overcoming some major limitations of the conventional chemotherapy. In conclusion, co-delivery of gene and chemotherapy drugs with cationic liposome delivery system will play a vital role in the future and especially could be a promising clinical treatment for drug-resistant tumors.

Does Soil Treated with Conidial Formulations of Trichoderma spp. Attract or Repel Subterranean Termites?

Previous studies showed that many wood-rotting fungi were attractive to termites; however, little attention has been paid to the relationship between termites and soil fungus. In the present study, different designs of two-choice tests were conducted to investigate the behaviors of two subterranean termites, Coptotermes formosanus Shiraki (wood-feeding lower termites) and Odontotermes formosanus (Shiraki) (fungus-growing higher termites), in response to soil (or sand) treated with the commercial conidial formulations of Trichoderma harzianum Rifai (BioWorks) and Trichoderma viride Pers. ex Fries (Shuiguxin). The short-term (1 d) choice tests showed no significant difference in termite aggregation (C. formosanus and O. formosanus) between treated and untreated soil, regardless of Trichoderma species and concentrations. However, in the long-term choice tests, C. formosanus consumed significantly more wood in the chambers containing soil treated with the conidial formulation of T. viride (1 × 108 conidia/g) than that containing untreated soil. The tunneling choice tests showed that sand treated with T. viride (1 × 106 or 1 × 108 conidia/g) or T. harzianum (1 × 106 conidia/g) significantly increased the tunneling activities of C. formosanus. However, sand treated with T. viride (1 × 106 or 1 × 108 conidia/g) had a repellent effect on O. formosanus. Our study showed that the two subterranean termites behaved differently when responding to the conidial formulations of Trichoderma.

Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.

Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications. The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan. The cationic liposome was composed of 3ß-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan. The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay. The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA. Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone. In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.