Comparison of viral elution-concentration methods for recovering noroviruses from deli meats.

This study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats. Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (PEG 6000) precipitation, skimmed milk flocculation (SMF), TRIzol® reagent, and a combination of PEG/TRIzol® and SMF/TRIzol® methods. Molecular analysis using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) revealed TRIzol® as the best method to be used for viral recovery from ham with medium recovery rates of 37.6% for NoV GI and 50.1% for NoV GII. Viral recovery from turkey meat showed medium recovery rates of 14.4% for NoV GI and 8.9% for NoV GII. For MNV-1, the rates varied from 0.5% to 80.8% not only according to the matrix but also with the associated virus and its inoculum (NoV GI or GII). The monitoring of commercial samples obtained in the Great Metropolitan region of Rio de Janeiro in order to demonstrate the occurrence of NoV GI and GII contamination in both matrices was also performed in 60 samples. NoV GI or GII were not detected in any samples, while MNV-1 used as the sample process control viruswas successfully recovered in 100% of samples.

Evaluación de la resistencia a la tracción de tres dispositivos ortodóncicos instalados con resina autopolimerizable en premolares: un estudio in vitro / Evaluation of the tensile strength of three orthodontic devices installed with self-curing resin in premolars: an in vitro study

Las tracciones orto-quirúrgicos son técnicas ampliamente utilizadas en odontología para la reposición en oclusión de dientes que incluidos. Nuestro objetivo fue evaluar la resistencia a la tracción de tres tipos de dispositivos de tracción. En este estudio in vitro fueron utilizados 45 premolares sanos, conservados inicialmente en suero fisiológico al 0,9%; fueron divididos en tres grupos, de los cuales 15 recibieron la instalación de brackets, 15 recibieron botones ortodóncicos y 15 recibieron la instalación de mallas. La adhesión fue realizada con resina de autocurado y en todos ellos se instaló un alambre ortodóncico de 0,30mm; los dientes fueron fijados con resina acrílica y almacenados en suero fisiológico al 0,9% por 72 horas. A continuación fueron sometidos a test de tracción mecánica, con una tracción máxima de 50N, siendo analizados con del programa Logger Pro®. Los brackets demostraron mayor resistencia a la tracción (44,6N), seguidos por los botones y finalmente por las mallas (32N). La principal área de falla fue la interface dispositivo/resina, siendo las mallas las que presentaron el mayor índice de fracaso (66%). Ningún alambre presentó ruptura en los análisis ejecutados. Las mallas presentan los resultados mecánicos más pobres cuando son comparados con botones y brackets; el área de ruptura más frecuente corresponde a la interface dispositivo/resina

Ortho-surgical tractions are techniques very used in dentistry for the reposition in occlusion of embedded teeth. The aim of this research was to evaluated the resistance to traction of three kind of devices used in a traction technique. In this in vitro research was used 45 bicuspid, initially maintained in saline solution (0,9%); were formed three groups, where 15 received brackets, 15 received button and 15 received mesh. The adhesion was do it with self-curing resin and in all of them was installed an orthodontics wire of 0,30 mm; the teeth was fixed with acrylic resin and maintained in saline solution (0,9%) for 72 hours. Follow that, the samples were submitted to mechanical traction test, with maximum traction of 50N, being analyzed with Logger Pro software. The brackets show more resistance to the traction (44,6N), follow that button and finally for mesh (32N). The principal areas of fracture was to device/resin interface, presented the mesh a more failure percentage (66%). Non wire presented fracture in the analyses. The mesh present poor results when compared to buttons and brackets; the more prevalent area failure was device/resin interface