1.
Biochem Biophys Res Commun
; 389(4): 684, 2009 Nov 27.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19766593
2.
ACS Appl Mater Interfaces
; 4(11): 5945-8, 2012 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23039103
RESUMO
Self-assembly of thiol-modified oligonucleotides on Au films has great importance for biosensor applications. Prior to the self-assembly, a piranha treatment (PT) is commonly used to clean the Au surface. Here we report that near-surface oxidized sulfur modifications on Au thin films by PT for longer than 60 s have serious effects on the self-assembled monolayer (SAM) formation of thiol-modified single-stranded thrombin binding aptamer (s-TBA), and a PT time of 10-30 s is optimal for s-TBA SAM formation. These results have important implication to SAM formation of biomolecules, especially for the thiol-modified ones where a careful consideration of this key step could significantly enhance the SAM formation and biosensor performance.