RESUMO
Saliva is an underused fluid with considerable promise for biomedical testing. Its potential is particularly great for monitoring small-molecule analytes since these are often present in saliva at concentrations that correlate well with their free levels in blood. We describe the development of a prototype diagnostic device for the rapid detection of the antiepileptic drug (AED) phenytoin in saliva. The multicomponent system includes a hand-portable surface plasmon resonance (SPR) imaging instrument and a disposable microfluidic assay card.
Assuntos
Saliva/química , Ressonância de Plasmônio de Superfície , Biomarcadores/análise , Biomarcadores/química , Biomarcadores/metabolismo , Microfluídica , Ressonância de Plasmônio de Superfície/instrumentação , Ressonância de Plasmônio de Superfície/métodosRESUMO
This paper describes an approach for fabricating multi-layer microfluidic systems from a combination of glass and plastic materials. Methods and characterization results for the microfabrication technologies underlying the process flow are presented. The approach is used to fabricate and characterize multi-layer plastic/glass microfluidic systems containing electrical and mechanical functionality. Hot embossing, heat staking of plastics, injection molding, microstenciling of electrodes, and stereolithography were combined with conventional MEMS fabrication techniques to realize the multi-layer systems. The approach enabled the integration of multiple plastic/glass materials into a single monolithic system, provided a solution for the integration of electrical functionality throughout the system, provided a mechanism for the inclusion of microactuators such as micropumps/valves, and provided an interconnect technology for interfacing fluids and electrical components between the micro system and the macro world.
Assuntos
Vidro/química , Membranas Artificiais , Microfluídica/instrumentação , Plásticos/química , Materiais Biocompatíveis/química , Condutividade Elétrica , Desenho de Equipamento , Microeletrodos , Microscopia Eletrônica de Varredura , Fotografação/métodos , PressãoAssuntos
Ouro/química , Membranas Artificiais , Nanopartículas/química , Nanopartículas/ultraestrutura , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Fotoquímica/métodos , Cristalização/métodos , Temperatura Alta , Íons , Substâncias Macromoleculares/química , Conformação Molecular , Nanotecnologia/métodos , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
The development of a microneedle-based biosensor array for multiplexed in situ detection of exercise-induced metabolic acidosis, tumor microenvironment, and other variations in tissue chemistry is described. Simultaneous and selective amperometric detection of pH, glucose, and lactate over a range of physiologically relevant concentrations in complex media is demonstrated. Furthermore, materials modified with a cell-resistant (Lipidure(®)) coating were shown to inhibit macrophage adhesion; no signs of coating delamination were noted over a 48-h period.