Structural basis for interorganelle phospholipid transport mediated by VAT-1.

Eukaryotic cells are compartmentalized to form organelles, whose functions rely on proper phospholipid and protein transport. Here we determined the crystal structure of human VAT-1, a cytosolic soluble protein that was suggested to transfer phosphatidylserine, at 2.2 Å resolution. We found that VAT-1 transferred not only phosphatidylserine but also other acidic phospholipids between membranes in vitro Structure-based mutational analyses showed the presence of a possible lipid-binding cavity at the interface between the two subdomains, and two tyrosine residues in the flexible loops facilitated phospholipid transfer, likely by functioning as a gate to this lipid-binding cavity. We also found that a basic and hydrophobic loop with two tryptophan residues protruded from the molecule and facilitated binding to the acidic-lipid membranes, thereby achieving efficient phospholipid transfer.

Relationships between skeletal morphology and patterns of bilateral agenesis of third molars in Japanese orthodontic patients.

The aim of this study was to reveal the correlations between bilateral agenesis of third molars (M3s) and skeletal morphology in Japanese male and female orthodontic patients. Sixty patients (30 males, 30 females), with bilateral agenesis of maxillary M3s and without agenesis of mandibular M3s (group U), and 60 patients (30 males, 30 females), with bilateral agenesis of mandibular M3s and without agenesis of maxillary M3s (group L), were selected as agenesis groups. Additionally, 60 patients (30 males, 30 females) with all four M3s were selected as the control group (group C). Patients in these three groups had no agenesis of teeth other than M3s. Lateral cephalograms of each patient were used to evaluate skeletal morphology of the maxilla and mandible. Two-way analysis of variance was used for statistical comparisons. Groups U and L had significantly smaller maxillary length and area than group C. Group U exhibited a significantly smaller lower facial height than group C. Males showed significantly larger maxillary length; total mandibular and mandibular body length; mandibular ramus height; SNB angle; maxillary area; and mandibular symphysis, corpus and ramus areas than females. Females had significantly larger lower facial height, gonial angle and ANB angle than males. Smaller maxillary length and area and lower facial height should be considered in planning orthodontic treatment for patients with bilateral agenesis of maxillary and mandibular M3s.

Size and bridging of the sella turcica in Japanese orthodontic patients with tooth agenesis.

The purpose of this study was to investigate the association between the size and bridging of the sella turcica and tooth agenesis, and whether the likelihood of second premolar agenesis can be predicted from the sella turcica size and bridging in Japanese orthodontic patients. Patients were divided into four groups of 32: groups 1 and 2 consisted of patients with agenesis of the maxillary and mandibular second premolars, respectively; group 3, patients with severe tooth agenesis; and group 4, patients without tooth agenesis. Each group was divided into two subgroups of 16 each based on the patient's age: patients under 14 years of age (groups 1A through 4A, group A) and patients 14 years of age or older (groups 1B through 4B, group B). Lateral cephalograms were used to evaluate the size and bridging of the sella turcica. The interclinoidal distance (ID) was significantly shorter in groups 1 and 3 than in group 4, and in group 3 than in group 2. Group B exhibited significantly greater depth, diameter, area, and perimeter of the sella turcica than group A. Groups 3 and 1B had a significantly higher prevalence of sella turcica bridging than groups 4 and 4B, respectively. Maxillary second premolar agenesis and severe tooth agenesis were associated with a reduced ID irrespective of age and increased occurrence of sella turcica bridging. The early emergence in life of a short ID might be a predictor of possible second premolar agenesis in later life.

Correction to: Use of the Er,Cr:YSGG laser for removing remnant adhesive from the enamel surface in rebonding of orthodontic brackets.

In the original publication of the article, Fig. 2 was published incorrectly.

Use of the Er,Cr:YSGG laser for removing remnant adhesive from the enamel surface in rebonding of orthodontic brackets.

The aim of this study was to compare the use of the Er,Cr:YSGG laser with the use of adhesive-removing pliers for removing remnant adhesive from the enamel surface. A total of 54 sound premolars were divided into two groups of 27. Each group was assigned one of two adhesive removal systems: the Er,Cr:YSGG laser, and adhesive-removing pliers. Shear bond strength was measured 24 h after bracket bonding, with the bracket bonding/debonding and residual adhesive removal procedures repeated twice after the first adhesive removal. Before the first bonding and after each adhesive removal procedure, the average surface roughness (Ra) of each tooth was measured. Additionally, 14 of the premolars were examined under a scanning electron microscope. There were no significant differences in the shear bond strength between the two removal systems or between the three debonding sequences. There were significant differences in the enamel surface roughness after each removal sequence between the adhesive removal systems, and a significant increase in the enamel surface roughness was noted in each group with successive removal sequences. The scanning electron microscope images revealed evaporation of the primer and adhesive on the enamel surface and laser etching of enamel in the laser removal group, and the remnants of primer and adhesive on the enamel surface after each removal sequence in the plier removal group. The Er,Cr:YSGG laser was useful for removing remnant adhesive from the enamel surface for bracket rebonding.

Maxillary sinus size and posterior tooth inclination in Japanese orthodontic patients with agenesis of maxillary second premolars.

The purpose of this study was to investigate the expansion of the maxillary sinus and the inclinations of posterior teeth in orthodontic patients with maxillary second premolar agenesis. A total of 30 subjects with one or two congenitally missing maxillary second premolars and retained maxillary deciduous second molars (a agenesis group) were selected and divided into a unilateral agenesis group (20 subjects with one maxillary second premolar missing) and a bilateral agenesis group (10 subjects with two maxillary second premolars missing). As controls, 30 sex- and age-matched subjects without agenesis of the maxillary second premolars were selected. Oblique cephalograms were used to investigate the association of maxillary second premolar agenesis and lower maxillary sinus size and posterior tooth inclinations. Agenesis of the maxillary second premolars induced significantly large lower maxillary sinus length, depth, area and mesial inclination of the maxillary first premolar, a significantly small anterior maxillary length, and a significantly more distal position for root apex of the maxillary first premolar. There were no significant differences in any measurements of the lower maxillary sinus and posterior teeth between the non-agenesis side in the unilateral agenesis group and the control group. Maxillary second premolar agenesis caused inferior and anterior expansion of the lower maxillary sinus and the mesial inclination of the maxillary first premolars with a distal position of root apex. Unilateral agenesis of the maxillary second premolar did not affect on the lower maxillary sinus size or posterior tooth inclinations of the unaffected antimere.

Association between skeletal morphology and agenesis of all four third molars in Japanese orthodontic patients.

The purpose of this study was to clarify differences in skeletal morphologies between male and female orthodontic patients with and without agenesis of all four third molars. A total of 64 patients (32 males and 32 females) with agenesis of all four third molars without agenesis of other teeth were selected as the third molars agenesis group (group 1). In addition, 64 patients (32 males and 32 females) with all these teeth were selected as controls (group 2). Lateral cephalograms taken between the ages of 14 and 30 years were used to compare skeletal morphology between groups 1 and 2 and between sexes. Maxillary length (P < 0.001), lower facial height (P < 0.05), gonial angle (P < 0.001) and mandibular plane angle (P < 0.001) were significantly smaller in group 1 than in group 2. Irrespective of the presence or absence of all four third molars, males had significantly smaller lower facial height (P < 0.01) and mandibular plane angle (P < 0.001) and significantly greater total mandibular length (P < 0.001), mandibular body length (P < 0.001) and mandibular ramus height (P < 0.001) than females. Japanese orthodontic patients with agenesis of all four third molars had significantly small maxillary length, lower facial height, gonial angle and mandibular plane angle.

A novel import route for an N-anchor mitochondrial outer membrane protein aided by the TIM23 complex.

The membrane topology of Om45 in the yeast mitochondrial outer membrane (OM) is under debate. Here, we confirm that Om45 is anchored to the OM from the intermembrane space (IMS) by its N-terminal hydrophobic segment. We show that import of Om45 requires the presequence receptors, Tom20 and Tom22, and the import channel of Tom40. Unlike any of the known OM proteins, Om45 import requires the TIM23 complex in the inner membrane, a translocator for presequence-containing proteins, and the membrane potential (ΔΨ). Therefore, Om45 is anchored to the OM via the IMS by a novel import pathway involving the TIM23 complex.

Patterns of bilateral agenesis of maxillary third molars and agenesis of other teeth.

The purpose of this study was to investigate the patterns of bilateral agenesis of maxillary third molars and other tooth agenesis in Japanese orthodontic patients. A group of 262 subjects with bilateral agenesis of maxillary third molars (group A) was divided into three subgroups: group 1A consisting of 114 subjects without agenesis of mandibular third molars; group 2A, 31 subjects with unilateral agenesis of mandibular third molars; and group 3A, 117 subjects with agenesis of all third molars. As controls, 926 other subjects without third molar agenesis were selected (group C). Panoramic radiographs were mainly used to examine for tooth agenesis. The Chi square test and odds ratio were used to make statistical comparisons. The prevalence rates of agenesis of maxillary lateral incisors, mandibular incisors and maxillary and mandibular second premolars, and bilateral agenesis of maxillary lateral incisors and second premolars were significantly higher in any one of the third molar agenesis groups than in the control group. Characteristically, no significant increase in occurrence of bilateral agenesis of mandibular second premolars was demonstrated by the subjects with bilateral agenesis of maxillary third molars. Irrespective of whether unilateral or bilateral agenesis of mandibular third molars is present or not, the Japanese orthodontic patients with bilateral agenesis of maxillary third molars exhibited a significantly increased occurrence of unilateral or bilateral agenesis of maxillary lateral incisors, mandibular incisors and maxillary and mandibular second premolars, except for bilateral agenesis of mandibular incisors and second premolars.

Association between third molar agenesis patterns and agenesis of other teeth in a Japanese orthodontic population.

The purpose of this study was to investigate the patterns of third molar agenesis and incidence of agenesis of other permanent teeth in a Japanese orthodontic patient group. A total of 417 Japanese subjects (134 males and 283 females) with agenesis of one or more third molars were divided into four groups according to the agenesis pattern, and 874 other Japanese subjects (302 males and 572 females) without third molar agenesis were assigned to a control group. Panoramic radiographs and medical and dental records were used to examine for tooth agenesis. The Chi-square test and odds ratio were used to make statistical comparisons. The prevalence of third molar agenesis worked out at 32.3% with no statistically significant gender difference. A high prevalence rate of agenesis of third molars, unilateral or bilateral, could be considered characteristic of the Japanese orthodontic population. Significant increases in occurrence of oligodontia, and unilateral or bilateral agenesis of other teeth, including maxillary lateral incisors and maxillary and mandibular second premolars, were observed in all or almost all of the third molar agenesis groups, compared with the controls. A significantly increased prevalence rate of mandibular lateral incisor agenesis was observed in almost all of the third molar agenesis groups. The Japanese patients with third molar agenesis had a significantly increased occurrence of oligodontia, and unilateral or bilateral agenesis of maxillary and mandibular lateral incisors and second premolars, except for bilateral agenesis of mandibular lateral incisors.

Identification of marker proteins by orthodontic treatment: relationship of RANKL in the gingival crevicular fluid and of amylase in whole saliva with orthodontic treatment.

Orthodontic medical treatment is performed to move a tooth to the optimal position to obtain optimal occlusion. Orthodontic treatment is accompanied by mechanical stress due to orthodontic force and by psychological stress that is experienced as pain or displeasure. The purpose of this study was to identify stress marker proteins during orthodontic treatment. Levels of receptor activator of NFκB (RANKL) and heat shock protein 70 (HSP70) in the gingival crevicular fluid (GCF) were analyzed as markers of mechanical stress, and levels of chromogranin A (CgA) and amylase in whole saliva were analyzed as markers of psychological stress. GCF was collected from control and experimental teeth at initiation of treatment and 24 h after treatment. Whole saliva was collected before treatment, at initiation of treatment and 24 h after treatment. RANKL was expressed at 24 h after treatment in the experimental GCF, but not in the control GCF. HSP70 appeared to be constitutively expressed in GCF, and its levels showed no major change between the control and experimental groups from initiation of treatment to 24 h after treatment. Amylase activity in whole saliva was enhanced at 24 h after treatment compared to control, but CgA levels showed little change between the groups. These results indicated that RANKL and amylase may be the candidate markers for mechanical and psychological stress, respectively, during orthodontic treatment, even though the total protein concentration and amylase activity displayed a large standard deviation among subjects. Further studies are therefore required to establish these markers for clinical use.

Patterns of tooth agenesis in Japanese subjects with bilateral agenesis of mandibular second premolars.

The aim of this study was to explore the patterns of tooth agenesis in Japanese orthodontic patients with bilateral agenesis of mandibular second premolars. A total of 80 Japanese orthodontic patients with 2 congenitally missing mandibular second premolars were selected as the subjects of this study (experimental group). Another 80 individuals without bilateral agenesis of mandibular second premolars were collected for comparison (control group). The 2 groups were matched with regard to sex. Radiographs, dental casts and records of medical and/or dental treatment were used to identify tooth agenesis. The Chi-square test, odds ratio, and test for equality were used to make statistical comparisons. The prevalence rates of other types of tooth agenesis were significantly higher in the experimental group than in the control group. The occurrence of agenesis of maxillary and mandibular lateral incisors and third molars, and maxillary second premolars was also significantly higher in the experimental group than in the control group. Significantly increased prevalence rates of symmetrical tooth agenesis, with third molars included, and oligodontia were observed in the experimental group. Japanese subjects with bilateral agenesis of mandibular second premolars are at significantly high risk of agenesis of other types of permanent teeth, symmetrical tooth agenesis and oligodontia.

Short-term treatment effects of quad-helix on maxillomandibular expansion in patients with maxillary incisor crowding.

The purpose of this study was to evaluate the effects of the maxillary arch expansion on maxillomandibular arch widths in patients treated with the quad-helix versus untreated controls. The treatment group consisted of 50 consecutive patients treated for maxillary incisor crowding with a quad-helix appliance in the early mixed dentition. Lateral cephalograms and dental casts taken at the start (T0) and end (T1) of the quad-helix treatment were obtained. The control group consisted of 50 untreated patients with the same type of malocclusion. Two consecutive lateral cephalograms and dental casts of each untreated patient were taken at about the same time as T0 and T1. All these study materials were analyzed for comparison between the two groups. The mean ages at T0 and T1 in the two groups were about the same. The maxillary first molars moved and tipped distally in the treatment group and mesially in the control group. The quad-helix treatment actually expanded the mandibular and maxillary arches concurrently. The more the maxillary arch widths were expanded and the less the maxillary first molars were inclined distally, the more the mandibular arch widths were expanded. The quad-helix activation caused lingual tipping and mesiobuccal rotation of the maxillary first molars. The mesiobuccal rotation of the maxillary first molars could turn molar occlusal relationships for the better from Class II to Class I. The quad-helix treatment gives rise to spontaneous expansion of the mandibular arch concurrent with maxillary expansion in the early mixed dentition patients with maxillary incisor crowding.

Effects of tooth bleaching on shear bond strength of brackets rebonded with a self-etching adhesive system.

The purpose of this study was to ascertain the effects of tooth bleaching on the shear bond strength of orthodontic brackets rebonded with a self-etching adhesive system. A total of 39 premolars were collected and divided into three equal groups: in group 1 bracket bonding was performed without bleaching treatment; specimens in group 2 were bonded immediately after bleaching; and group 3 teeth were bleached, then immersed in artificial saliva and left for 7 days before bonding. The shear bond strength was measured, with the bonding/debonding procedures repeated once after the first debonding, and the bracket/adhesive failure modes were evaluated by the adhesive remnant index after each debonding. Excepting the mean shear bond strength for group 2 after the first debonding, the overall mean values reached the minimum clinical requirement of 6 MPa. The mean values at the first and second debondings were significantly higher in groups 1 and 3 than in group 2. Between groups 1 and 3, significant differences were noted at the first debonding, but not at the second debonding. Group 2 showed significant differences in mean shear bond strength between the first and second debondings. Bond failure at the enamel-adhesive interface occurred more frequently in group 2 than in groups 1 or 3 after the first debonding. The bracket-rebonding procedure can recover the reduced shear bond strength caused by immediate bonding after bleaching to a clinically acceptable level, but not to the prebleaching level.

Shear bond strength of rebonded brackets after removal of adhesives with Er,Cr:YSGG laser.

This study was conducted to examine the bond strength of rebonded orthodontic brackets after adhesive residuals on the surface of the bracket bases were removed by Er,Cr:YSGG lasers. Seventy-six brackets bonded to premolars with a self-etching primer adhesive system were equally divided into four groups after the first debonding with the bracket bases (Group 1) untreated, and treated by (Group 2) Er,Cr:YSGG laser, (Group 3) sandblaster, and (Group 4) Er,Cr:YSGG laser/sandblaster. The treated brackets were rebonded to the new premolars in the same manner as the first-stage experiment. The shear bond strengths were measured, with the bonding/debonding procedures repeated once after the first debonding, and the bracket/adhesive failure modes were evaluated after each debonding. The treated bracket base surfaces were observed under a scanning electron microscopy (SEM). The mean rebond strengths were significantly lower in group 1 than in other groups, and there were no significant differences between the other groups. The mean initial bond strength was significantly higher than the mean rebond strength in group 1 but there was no significant difference between the two in the other three groups. Failures at the bracket-adhesive interface occurred frequently at second debonding in group 1. Under the SEM, residual adhesive was removed from the bracket bases by Er,Cr:YSGG laser, while adhesive remnant was seen underneath the meshwork of the bracket bases and microroughness appeared on the meshwork after sandblasting. Er,Cr:YSGG laser certainly could serve the purpose of promoting the use of recycled orthodontic brackets.

Effects of plastic bracket primer on the shear bond strengths of orthodontic brackets.

BACKGROUND/PURPOSE: To assess the usefulness of plastic bracket primer (PBP) for improving the bond strength of plastic brackets (PBs) using three types of orthodontic brackets, including PBs, metal brackets (MBs), and ceramic brackets (CBs). MATERIALS AND METHODS: A total of 162 premolars were gathered and divided equally into six groups of 27. Three groups were tested with the application of PBP (PB+, MB+, and CB+), and three groups were tested without primer (groups PB-, MB-, and CB-). All the groups were bonded using BeautiOrtho Bond II self-etching adhesive. The shear bond strength (SBS) was measured and the bond failure mode was evaluated using the adhesive remnant index after debonding. RESULTS: There were significant differences in the mean SBS between groups PB-, MB and CB-, between PB+ and CB+, and between MB+ and CB+. Group PB + had a significantly higher mean SBS than group PB-. The occurrence of bond failure at the enamel and adhesive interface was more frequent in groups PB+ and CB- than in group PB-; and in groups PB+ and CB + than in group MB+. CONCLUSION: Plastic bracket primer can increase the bond strength of PBs to the level of metal brackets, but not to the level of ceramic brackets.

Shear bond strength of orthodontic brackets bonded with resin coating material.

The purpose of this study was to determine whether a system using a resin coating material (PRG Barrier Coat) with anticariogenic ability can effectively bond orthodontic brackets to human teeth. Resin-modified glass-ionomer cement system (Fuji Ortho LC, group 1) and resin composite cement systems (BeautyOrtho Bond) combined with a self-etching primer (group 2), with the resin coating material (group 3), and with the resin coating material after an organic acid etching agent (group 4) were used for bracket bonding. The mean shear bond strength (SBS) was significantly higher in group 1 than in groups 2, 3 and 4. Groups 2 and 4 exhibited a significantly higher mean SBS than group 3. The resin composite cement system combined with the resin coating material after the organic acid etching agent can serve as an alternative for orthodontic bracket bonding.

Bond strength of indirect bonded brackets in orthodontic adhesives with different viscosities.

The aim of this study was to assess the effects of three adhesives with different viscosities and an adhesion promoter on the shear bond strength (SBS) of orthodontic brackets bonded to human premolars with an indirect bonding system (IDBS). High, medium and low viscosity IDBSs with and without application of the adhesion promoter were used. The mean SBSs of the high and low viscosity IDBSs were significantly higher than that of the medium viscosity IDBS. Application of the adhesion promoter significantly increased the SBSs. The adhesion promoter significantly increased the surface roughness and free-energy of enamel. Irrespective of application or nonapplication of the adhesion promoter, the high and low viscosity IDBSs are effective for bracket bonding. Use of the medium viscosity IDBS in combination with the adhesion promoter is recommended for obtaining a clinically acceptable SBS.

Cephalometric evaluation of maxillary sinus sizes in different malocclusion classes.

We found few studies on the association between maxillary sinus size and malocclusion in an electronic search using PubMed. The purpose of this study was to investigate maxillary sinus size in different malocclusion groups and the association between maxillary sinus size and dentofacial morphology by the use of lateral cephalometric radiographs. A total of 120 lateral cephalograms were used. These radiographs were derived from subjects with skeletal Class I, Class II, and Class III malocclusions, classified on the basis of the A-N-B angle. Each malocclusion group consisted of 20 boys and 20 girls ranging in age from 12 to 16 years. Two linear measurements and three area measurements were made to evaluate maxillary sinus size, and four angular and eight linear measurements were made to evaluate dentofacial morphology. Analysis of variance and Pearson's correlation analysis were performed for statistical comparison. The maxillary sinuses showed no significant differences in size between the different classes of skeletal malocclusion or between sexes. However, the maxillary sinus measurements were significantly correlated with several dentofacial morphological measurements. When formulating an orthodontic treatment plan, orthodontists should take into consideration the fact that the patients 12 to 16 years old with large cranial bases and nasomaxillary complexes tend to have larger maxillary sinuses, but there is no significant association between maxillary sinus size and the A-N-B angle denoting the sagittal skeletal jaw relationship.

Effects of premolar extractions on Bolton overall ratios and tooth-size discrepancies in a Japanese orthodontic population.

INTRODUCTION: The purpose of this study was to investigate the effects of premolar extractions on the Bolton overall ratios and overall tooth-size discrepancies in a Japanese orthodontic population. METHODS: Mesiodistal tooth widths were measured on 198 pretreatment dental casts of subjects with Class I, Class II, and Class III malocclusions. The overall ratios and tooth-size discrepancies were determined before and after hypothetical premolar extractions. Before and after extractions, the subjects were divided into small, normal, and large overall ratio groups categorized by the Bolton standard deviation definition, and into small, normal, and large correction groups by the actual amount of change calculated for tooth-size correction in millimeters. Extractions were performed in the following combinations: (1) all first premolars, (2) all second premolars, (3) maxillary first and mandibular second premolars, and (4) maxillary second and mandibular first premolars. For statistical evaluations, analysis of variance, Kruskal-Wallis, Friedman, Scheffé, Bonferroni, and Tukey tests were performed. RESULTS: The overall ratios decreased in every malocclusion group after extraction of any combination of premolars. The decreases were significantly notable in combinations 2 and 4. Some subjects in the normal overall ratio and maxillary and mandibular correction groups moved into the clinically significant tooth-size discrepancy group after premolar extraction, and the reverse was also true. CONCLUSIONS: In formulating a treatment plan involving premolar extractions, orthodontists should consider that the overall ratios might decrease, and normal and clinically significant tooth-size discrepancies could change mutually after extractions.