Initial oral biofilm formation on titanium implants with different surface treatments: An in vivo study.

OBJECTIVE: The aim of this study was to examine in vivo the initial bacterial adhesion on titanium implants with different surface treatments. DESIGN: Ten subjects wore oral splints containing machined pure titanium disks (Ti-M), acid-etched titanium (Ti-AE) and anodized and laser irradiated disks (Ti-AL) for 24h. After this period, disks were removed from the splints and adherent bacteria were quantified by an enzymatic assay to assess total viable bacteria and by Real Time PCR to evaluate total bacteria and Streptococcus oralis levels. Additionally, the initial adherent microorganisms were visualized by scanning electron microscopy (SEM). Titanium surface morphology was verified using SEM, and roughness was evaluated by profilometer analysis. RESULTS: Regarding titanium surface roughness, Ti-AL (1.423±0.397) showed significantly higher Ra values than did Ti-M (0.771±0.182) and Ti-AE (0.735±0.196) (p<0.05, ANOVA - Tahame). Ti-AE and Ti-AL presented roughened micro-structure surfaces characterized by open pores, whereas Ti-M showed long grooves alternating with planed areas. Comparing the Ti-M, Ti-AE and Ti-AL groups for viable bacteria (MTT assay), total bacteria and S. oralis quantification (qPCR), no significant differences were observed among these three groups (p>0.05, ANOVA - Tahame). SEM images showed similar bacterial adhesion on the three titanium surfaces, predominantly characterized by cocci and several bacilli, indicating an initial colonization of the oral biofilm. CONCLUSION: In conclusion, roughness and microtopography did not stimulate initial biofilm formation on titanium surfaces with different surface treatments.

La displasia cemento ósea florida y su diagnóstico diferencial / The Florid cemento-bony dysplasia and the differential diagnosis

Las lesiones benignas fibro-óseas de los maxilares constituyen un grupo diverso de enfermedades con una característica histológica común: la sustitución de hueso normal por tejido compuesto de colágeno y fibroblastos, con cantidades variables de una sustancia mineral que puede ser de hueso, cemento o ambos. Estas lesiones incluyen la displasia fibrosa, la displasia cemento-ósea en sus variantes: periapical, focal o florida, el cementoma gigantiforme familiar y el fibroma osificante cemento-osificante. La osteomielitis es un proceso inflamatorio agudo o crónico de los espacios medulares o corticales del hueso, que se extiende más allá del sitio inicial de desenvolvimiento. La osteomielitis esclerosante difusa es más frecuente en adultos, principalmente en la mandíbula. Se presenta radiográficamente como una lesión radiopaca difusa adyacente a los dientes, que puede ser multifocal. El objetivo de este trabajo fue describir un caso inusual de lesión fibro-ósea, cuyo diagnóstico diferencial se hizo también con la osteomielitis de los maxilares. Se concluyó que las lesiones fibro-óseas benignas, presentaron muchas similitudes con respecto a sus aspectos clínicos, radiográficos e histológicos. Por lo tanto, es fundamental el análisis conjunto de estas informaciones para obtener un diagnóstico definitivo(AU)

The benign fibrous-bony lesions are a diverse group of diseases with common features: replacement of normal bone by tissue composed of collagen and fibroblasts, with variable amounts of a mineral substance that could be bone, cement or both. These lesions include the fibrous dysplasia, the periapical cement-bony dysplasia, focal or florid, familiar giant cementoma and ossifying fibroma (cement-ossifying). Osteomyelitis is an acute or chronic inflammatory process of medullar or cortical spaces of bone extending beyond the onset site of development. Diffuse sclerosing osteomyelitis involves to adults mainly the mandible and radiographically is a diffuse radiopaque lesion adjacent to teeth and could be multifocal. The aim of present paper is to describe an uncommon case of fibrous-bony lesion also diagnosed with maxillary osteomyelitis. We conclude that the above mentioned lesions are very similar regarding its clinical, radiographic and histological features. Thus, it is fundamental the combined analysis of these informations to obtain a definite diagnosis(AU)

Papilomavírus humano como fator de risco para o carcinoma bucal e de orofaringe / Human papillomavirus as a risk factor for oral and oropharyngeal carcinoma

Introdução: Atualmente é bem aceito o papel de agentesinfecciosos como importantes fatores de risco para algumasneoplasias malignas. Na década de 1980, foi demonstrada aprimeira evidência do papilomavirus humano (HPV) como fator derisco para o CEC de boca. Desde então, centenas de pesquisasjá foram publicadas sobre esse assunto. Objetivo: O objetivodeste artigo foi, através de uma revisão da literatura, analisar asevidências científicas a respeito do HPV como fator de risco parao carcinoma bucal e de orofaringe. Método: O HPV é o principalagente infeccioso envolvido na patogênese do CEC bucal e deorofaringe, sendo o subtipo 16 o mais frequentemente encontrado.A partir desta revisão da literatura, fundamentada principalmenteem revisões sistemáticas e meta-análises. Conclusão: O HPV éum fator de risco independente para carcinomas espinocelularesde boca e orofaringe, principalmente o de tonsilas.