A Latex Metabolite Benefits Plant Fitness under Root Herbivore Attack.

Plants produce large amounts of secondary metabolites in their shoots and roots and store them in specialized secretory structures. Although secondary metabolites and their secretory structures are commonly assumed to have a defensive function, evidence that they benefit plant fitness under herbivore attack is scarce, especially below ground. Here, we tested whether latex secondary metabolites produced by the common dandelion (Taraxacum officinale agg.) decrease the performance of its major native insect root herbivore, the larvae of the common cockchafer (Melolontha melolontha), and benefit plant vegetative and reproductive fitness under M. melolontha attack. Across 17 T. officinale genotypes screened by gas and liquid chromatography, latex concentrations of the sesquiterpene lactone taraxinic acid ß-D-glucopyranosyl ester (TA-G) were negatively associated with M. melolontha larval growth. Adding purified TA-G to artificial diet at ecologically relevant concentrations reduced larval feeding. Silencing the germacrene A synthase ToGAS1, an enzyme that was identified to catalyze the first committed step of TA-G biosynthesis, resulted in a 90% reduction of TA-G levels and a pronounced increase in M. melolontha feeding. Transgenic, TA-G-deficient lines were preferred by M. melolontha and suffered three times more root biomass reduction than control lines. In a common garden experiment involving over 2,000 T. officinale individuals belonging to 17 different genotypes, high TA-G concentrations were associated with the maintenance of high vegetative and reproductive fitness under M. melolontha attack. Taken together, our study demonstrates that a latex secondary metabolite benefits plants under herbivore attack, a result that provides a mechanistic framework for root herbivore driven natural selection and evolution of plant defenses below ground.

A below-ground herbivore shapes root defensive chemistry in natural plant populations.

Plants display extensive intraspecific variation in secondary metabolites. However, the selective forces shaping this diversity remain often unknown, especially below ground. Using Taraxacum officinale and its major native insect root herbivore Melolontha melolontha, we tested whether below-ground herbivores drive intraspecific variation in root secondary metabolites. We found that high M. melolontha infestation levels over recent decades are associated with high concentrations of major root latex secondary metabolites across 21 central European T. officinale field populations. By cultivating offspring of these populations, we show that both heritable variation and phenotypic plasticity contribute to the observed differences. Furthermore, we demonstrate that the production of the sesquiterpene lactone taraxinic acid ß-D-glucopyranosyl ester (TA-G) is costly in the absence, but beneficial in the presence of M. melolontha, resulting in divergent selection of TA-G. Our results highlight the role of soil-dwelling insects for the evolution of plant defences in nature.

Pocket depth and bleeding on probing and their associations with dental, lifestyle, socioeconomic and blood variables: a cross-sectional, multicenter feasibility study of the German National Cohort.

BACKGROUND: To investigate the periodontal disease status in a multi-center cross-sectional study in Germany. Associations of dental, socio-economic, blood and biomedical variables with periodontal outcome parameters were evaluated. METHODS: From 4 different centers N = 311 persons were included, drawn randomly from the registration offices. Maximal pocket depth (PD) was used as primary indicator for periodontitis. It was classified as: no/mild ≤3 mm, moderate 4-5 mm, severe ≥6 mm. Associations between socioeconomic (household income, education), lifestyle, and biomedical factors and PD or bleeding on probing (BOP) per site ("Yes"/"No") was analyzed with logistic regression analysis. RESULTS: Mean age of subjects was 46.4 (range 20-77) years. A significantly higher risk of deeper pockets for smokers (OR = 2.4, current vs. never smoker) or persons with higher BMI (OR = 1.6, BMI increase by 5) was found. Severity of periodontitis was significantly associated with caries lesions (p = 0.01), bridges (p < .0001), crowns (p < .0001), leukocytes (p = 0.04), HbA1c (p < .0001) and MCV (p = 0.04). PD was positively correlated with BOP. No significant associations with BOP were found in regression analysis. CONCLUSIONS: Earlier findings for BMI and smoking with severity of PD were confirmed. Dental variables might be influenced by potential confounding factors e.g. dental hygiene. For blood parameters interactions with unknown systemic diseases may exist.

Abscisic acid-dependent regulation of small rubber particle protein gene expression in Taraxacum brevicorniculatum is mediated by TbbZIP1.

Natural rubber is a high-molecular-mass biopolymer found in the latex of >2,500 plant species, including Hevea brasiliensis, Parthenium argentatum and Taraxacum spp. The active sites of rubber biosynthesis are rubber particles, which comprise a hydrophobic rubber core surrounded by a phospholipid monolayer membrane containing species-dependent lipids and associated proteins. Small rubber particle proteins are the most abundant rubber particle-associated proteins in Taraxacum brevicorniculatum (TbSRPPs) and may promote rubber biosynthesis by stabilizing the rubber particle architecture. We investigated the transcriptional regulation of genes encoding SRPPs and identified a bZIP transcription factor (TbbZIP.1) similar to the Arabidopsis thaliana ABI5-ABF-AREB subfamily, which is thought to include downstream targets of ABA and/or abiotic stress-inducible protein kinases. The TbbZIP.1 gene was predominantly expressed in laticifers and regulates the expression of TbSRPP genes in an ABA-dependent manner. The individual TbSRPP genes showed distinct induction profiles, suggesting diverse roles in rubber biosynthesis and stress adaptation. The potential involvement of TbSRPPs in the adaptation of T. brevicorniculatum plants to environmental stress is discussed based on our current knowledge of the stress-response roles of SRPPs and their homologs, and the protective function of latex and rubber against pathogens. Our data suggest that TbSRPPs contribute to stress tolerance in T. brevicorniculatum and that their effects are mediated by TbbZIP.1.

Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of Taraxacum brevicorniculatum.

Certain Taraxacum species, such as Taraxacum koksaghyz and Taraxacum brevicorniculatum, produce large amounts of high-quality natural rubber in their latex, the milky cytoplasm of specialized cells known as laticifers. This high-molecular mass biopolymer consists mainly of poly(cis-1,4-isoprene) and is deposited in rubber particles by particle-bound enzymes that carry out the stereospecific condensation of isopentenyl diphosphate units. The polymer configuration suggests that the chain-elongating enzyme (rubber transferase; EC 2.5.1.20) is a cis-prenyltransferase (CPT). Here, we present a comprehensive analysis of transgenic T. brevicorniculatum plants in which the expression of three recently isolated CPTs known to be associated with rubber particles (TbCPT1 to -3) was heavily depleted by laticifer-specific RNA interference (RNAi). Analysis of the CPT-RNAi plants by nuclear magnetic resonance, size-exclusion chromatography, and gas chromatography-mass spectrometry indicated a significant reduction in rubber biosynthesis and a corresponding 50% increase in the levels of triterpenes and the main storage carbohydrate, inulin. Transmission electron microscopy revealed that the laticifers in CPT-RNAi plants contained fewer and smaller rubber particles than wild-type laticifers. We also observed lower activity of hydroxymethylglutaryl-coenzyme A reductase, the key enzyme in the mevalonate pathway, reflecting homeostatic control of the isopentenyl diphosphate pool. To our knowledge, this is the first in planta demonstration of latex-specific CPT activity in rubber biosynthesis.

Initial forces and moments delivered by removable thermoplastic appliances during rotation of an upper central incisor.

OBJECTIVE: To determine the forces and moments delivered to a maxillary central incisor during rotation by three different thermoplastic appliances with identical thickness. MATERIALS AND METHODS: Five identical appliances were manufactured from each of three materials (Ideal Clear 1.0 mm, Erkodur 1.0 mm, Biolon 1.0 mm). An upper central incisor fixed in a measuring device was rotated around its central axis in 0.5-degree steps to +/-2.5 degrees, +/-5 degrees, and +/-7.5 degrees (equivalent to an activation of +/-0.17 mm, +/-0.34 mm, and +/-0.51 mm of the incisor edge) in the clockwise and anticlockwise directions with the respective appliance fixed in place. For statistical analysis, the moments Tz (rotation) and forces Fz (intrusion) were tested. Means and standard deviations for Tz and median and 25% and 75% quantiles for Fz were calculated. An analysis of variance (ANOVA) was performed. RESULTS: The minimal moment was determined at a rotation of -0.17 mm (-7.3 Nmm, +/-0.8), and the maximal moment at a deflection of -0.51 mm (-71.8 Nmm. +/-2.5) was recorded. The minimal value for Fz was measured at an activation of -0.17 mm (0.0 N), and the highest intrusive forces were evaluated for a rotation of -0.51 mm (-5.8 N). The particular material sometimes had a significant (P < .05) influence on the forces delivered and the moments. CONCLUSIONS: During rotation with aligners, an intrusive force can also be observed. The direction of rotation, and the materials used all exert an influence on the force delivery properties of the appliance.

Torquing an upper central incisor with aligners--acting forces and biomechanical principles.

The forces delivered by aligners during torquing have still not been investigated. The purpose of this study was to measure the forces delivered to an upper central incisor during torquing with three different materials of the same thickness, and to describe the biomechanical principles of torquing with aligners. Five identical appliances were manufactured from each of three materials, all with a thickness of 1.0 mm (Ideal Clear®, Erkodur®, and Biolon®). An upper central incisor, as part of the measuring device, was torqued in defined steps in the vestibular and palatal directions with the respective appliance in place. For statistical analysis, the resulting forces, Fx (forces acting in the palatal and facial directions) and Fz (intrusive force as a side-effect) at a displacement of ±0.15 and ±0.8 mm from the tooth at the gingival margin were calculated. The mean Fx forces for ±0.15 mm displacement ranged from -1.89 N [standard deviation (SD) 0.48] to 0.11 N (SD 0.1). The mean Fz forces were between -0.97 N (SD 0.57) and -0.07 N (SD 0.22). The highest intrusive forces were measured during palatal displacement of the measuring tooth. An influence of direction of displacement on the levels of force was observed, especially for Fz at the greater displacement of ±0.8 mm. In relation to the intended amount of root movement during torquing, aligners tend to 'lift up' and therefore no effective force couple can be established for further root control. The force delivery properties are also influenced by the material used and the shape of the tooth.

Influence of thermoplastic appliance thickness on the magnitude of force delivered to a maxillary central incisor during tipping.

INTRODUCTION: The aim of the study was to quantify the forces delivered by thermoplastic appliances made of 2 materials with 2 thicknesses to a maxillary central incisor during tipping. METHODS: Two materials were tested, each in 2 thicknesses: Erkodur (Erkodent Erich Kopp GmbH, Pfalzgrafenweiler, Germany) 1.0 and 0.8 mm, and Biolon (Dreve Dentamid GmbH, Unna, Germany), 1.0 and 0.75 mm. For each material, 5 appliances were produced. To measure the forces applied, an isolated measuring tooth, part of a standardized resin model, was deflected in 0.05 degrees steps from 0 degrees to 0.42 degrees in the vestibular and palatine directions, after placing the respective appliance on the model. For statistical analysis, the force components Fx/tipping and Fz/intrusion at a displacement of +/- 0.151 mm from the incisor edge were selected. Means and standard deviations were calculated. The Wilcoxon 2-sample test for group pairings was used. RESULTS: The norms for the mean Fx forces ranged from 1.62 (SD, 0.41) to 5.35 N (SD, 0.63). The mean Fz forces were between 0.07 (SD, 0.13) and -2.47 N (SD, 0.34). The highest intrusive forces were measured during vestibular displacement of the measuring tooth. The forces delivered by the thick appliances were overall significantly higher (P <0.0001) than those of the thin materials. The forces delivered by the Biolon appliances were generally significantly higher (P <0.0001) than those for the Erkodur materials. CONCLUSIONS: The forces applied were mostly too high when compared with those stated in the literature as ideal. In addition to thickness, the thermoforming process influences the magnitude of the force delivered by a thermoformed appliance.

Initial forces generated by three types of thermoplastic appliances on an upper central incisor during tipping.

The force properties of thermoformed appliances have not been systematically investigated. Therefore, the aim of the present study was to quantify the forces delivered by thermoplastic appliances manufactured from three different materials, with the same thickness, on a central upper incisor, during tipping. Five identical appliances were manufactured from three different materials all with a thickness of 1.0 mm (Ideal Clear, Erkodur, and Biolon). For measuring the forces, an isolated measuring tooth, as part of a standardized resin model incorporated in a newly developed measuring device, was tipped in nine 2.7 arc minute (0.04629 degree) steps, from 0 to 0.416 degrees in the vestibular and palatal directions around a rotational axis through the virtual apex, after positioning an appliance on the model. For statistical analysis, the force components Fx/tipping and Fz/intrusion at a displacement of +/-0.151 mm from the incisor edge were determined. Means and standard deviations (SDs) were calculated. The Kruskal-Wallis test for overall effects and the Wilcoxon two-sample test for individual group pairings were used (P < 0.05 significance level). The mean Fx forces ranged from -2.82 N (SD 0.62) to 5.42 N (SD 0.56). The mean Fz forces were between -0.14 N (SD 0.52) and -2.3 N (SD 0.43). The highest intrusive forces were measured during vestibular displacement of the measuring tooth. The forces delivered by the Biolon appliance were found to be much greater (P < 0.01) than those of the other materials. The forces delivered by the materials investigated were mostly higher than those stated in the literature.

Periodontal Health and Use of Oral Health Services: A Comparison of Germans and Two Migrant Groups.

A cross-sectional study was performed with 251 individuals, consisting of 127 Germans, 68 migrants from Turkey, and 56 resettlers (migrants from the former Soviet Union with German ancestors) to compare periodontal health status, with a special focus on associations with lifestyle and anthropometric factors, and use of dental health services. Maximal pocket depth was used as a clinical surrogate marker for periodontitis. Other variables were obtained by questionnaires administered by a Turkish or Russian interpreter. The age- and sex-adjusted prevalence of periodontitis was significantly higher in Turks (odds ratio (OR) 2.84, 95% CI = 1.53-5.26) and slightly higher in resettlers (OR = 1.33, 95% CI = 0.71-2.49). These differences are partly explained by a differential distribution of known risk factors for periodontitis. A full model showed a higher prevalence of maximal pocket depth above 5 mm in Turks (OR = 1.97, 95% CI = 0.99-3.92). Use of oral health services was significantly lower in the two migrant groups. Individuals who reported regular visits to a dentist had significantly less periodontitis, independent of migrant status. A reasonable conclusion is that, since oral health causes major chronic diseases and has a major effect on total health system expenditures, public health efforts both generally and specifically focused on migrant groups are warranted.

The use of a neodymium-iron-boron magnet device for positioning a multi-stranded wire retainer in lingual retention--a pilot study in humans.

The aim of this study was to evaluate the time requirement of a newly developed device made of neodymium-iron-boron (NdFeB) magnets for positioning a multi-stranded, canine-to-canine retainer during bonding compared with dental floss and a transfer tray. Forty-five patients aged between 12 and 33 years (26 male, 19 female) previously treated with fixed appliances were enrolled in the study. The patients were randomly allocated to three groups (15 per group). For each group a mandibular canine-to-canine retainer of 0.018 inch Dentaflex multi-stranded wire (Dentaurum) was prefabricated for each patient on a cast. The bonding procedure was identical, except for the method of positioning the wire during adhesive fixation: group A dental floss, group B a small prefabricated transfer tray of dental resin and group C the NdFeB magnet device. For each group, the time required for the complete bonding process was measured. Kruskal-Wallis and Wilcoxon-Mann-Whitney tests were used for group and pairwise comparisons, respectively. The three methods required statistically significant different times (P < 0.001). The Wilcoxon-Mann-Whitney test revealed that wire positioning with the magnet device was significantly faster [4.98 minutes; standard deviation (SD) 0.68 minutes] than with dental floss (7.65 minutes, SD 1.14 minutes; P = 0.0001) or with transfer tray (5.75 minutes, SD 0.57 minutes; P = 0.001). The NdFeB magnet device is a timesaving appliance for positioning a multi-stranded, canine-to-canine retainer during bonding when compared with dental floss and an individually prefabricated transfer tray.

Morphological structures and protrusive cranial border guidance of the temporomandibular joint of Cercopithecus mona.

Morphological parameters of the temporomandibular joint (TMJ) of Cercopithecus mona were analyzed by sagittal medial/lateral slicing of the entire joint. The slice contours of the osseous structures of the joint surfaces were approximated by circles. In this manner, the main parameter of the protrusive cranial border guidance, the protrusive dimeric Link chain (DLC), could be measured. In each joint, all slices yielded protrusive DLCs which were nearly parallel to each other. In medial/lateral direction all parts of the joints participate in force transmission in initial protrusive cranial border function.

Ophthalmological manifestations of Parry-Romberg syndrome.

Parry-Romberg syndrome is a rare disease characterized by slowly progressive atrophy affecting facial subcutaneous tissues, including the underlying muscles and osteocartilaginous structures. Various periocular, ocular, and neuro-ophthalmological manifestations have been described in Parry-Romberg syndrome. The most common periocular disorders include enophthalmos, eyelid, and orbit alterations. The most frequent ocular disorders include corneal and retinal changes, and the most common neuro-ophthalmological disorders involve optic nerve, ocular motor and pupillary dysfunction. Besides the characteristic facial abnormalities, systemic manifestations may occur, including neurologic, dermatologic, cardiac, endocrine, infectious, orthodontic, and maxillofacial disorders. So far, mainly brief case reports describe these ophthalmological findings. Therefore, we summarize the ocular, periocular, and neuro-ophthalmological findings in detail, describe diagnostic modalities, and outline therapeutic options.

Prevalence of TMD symptoms in Turkish migrants and re-settlers from the former Soviet Union in comparison to a German group.

OBJECTIVES: The objective of this study was to investigate the prevalence of temporomandibular disorder (TMD) symptoms among Turks and re-settlers with German origin from Russia and to compare those findings with a German group from the same area. METHOD: Sixty-nine Turkish migrants, 50 re-settlers, and 96 Germans were clinically examined according to a short version of the Research Diagnostic Criteria (RDC/TMD) protocol. The subjects participated in a feasibility study of the German National Cohort and were recruited from the study center Heidelberg/Mannheim of the cluster Baden-Württemberg/Saarland. RESULTS: Significant differences emerged between the three ethnic groups for unassisted opening without pain, maximum unassisted opening, and overbite, with highest values for the German group. No significant differences were found for muscle pain on palpation or muscle and joint pain during opening. DISCUSSION: As the authors identified significant differences between the different ethnic groups for metric measurements, it might be beneficial to include questions concerning the ethnicity to the German version of the RDC/TMD for further research, to make the results more comparable.

Detection and size differentiation of simulated tooth root defects using flat-panel volume computerized tomography (fpVCT).

OBJECTIVE: Our aim was to evaluate the capacity of flat-panel volume computerized tomography (fpVCT) to enable the observer to detect and differentiate 3 different sizes of simulated tooth root defects in radiographs. STUDY DESIGN: Three simulated tooth root defects of different sizes and a defect-free area (160 sites in total) were randomly distributed on the buccal and lingual surface of 20 mandibular premolar roots of Sus scrofa domestica with round burs. For the imaging of the specimens, an fpVCT prototype was used. Findings were evaluated by 3 observers. RESULTS: Cavity 0 (no lesion) was correctly identified in 53%, cavity 1 in 69%, cavity 2 in 96%, and cavity 3 in 89%. Altogether, the simulated cavities were classified in a correct manner in 77%. The values were compared using receiver operating characteristic curves. The area under the curve (AUC) for cavity 0 versus the pooled results for cavities 1-3 was found to be 0.72. The AUC for the pooled results for 0-2 (no pathologic impact) versus cavity 3 (potential pathologic impact) was 0.94. There was no significant dependence of the results on the observer (P = .37). Results with P < .05 were considered to be significant. CONCLUSIONS: Flat-panel volume computerized tomography, which is currently used only as a research tool, has a high potential in detection and differentiation at an early stage of external root resorption cavities with pathologic relevance..

Imaging of the midpalatal suture in a porcine model: flat-panel volume computed tomography compared with multislice computed tomography.

OBJECTIVE: An investigation was conducted to compare the image quality of prototype flat-panel volume computed tomography (fpVCT) and multislice computed tomography (MSCT) of suture structures. STUDY DESIGN: Bone samples were taken from the midpalatal suture of 5 young (16 weeks) and 5 old (200 weeks) Sus scrofa domestica and fixed in formalin solution. An fpVCT prototype and an MSCT were used to obtain images of the specimens. The facial reformations were assessed by 4 observers using a 1 (excellent) to 5 (poor) rating scale for the weighted criteria visualization of the suture structure. A linear mixed model was used for statistical analysis. Results with P < .05 were considered to be statistically significant. RESULTS: The visualization of the suture of young specimens was significantly better than that of older animals (P < .001). The visualization of the suture with fpVCT was significantly better than that with MSCT (P < .001). CONCLUSION: Compared with MSCT, fpVCT produces superior results in the visualization of the midpalatal suture in a Sus scrofa domestica model.