Surface molecularly imprinted polymers based on magnetic multi-walled carbon nanotubes for the highly selective purification of resveratrol from crude extracts of Vitis vinifera, Arachis hypogaea, and Polygonum cuspidatum.

In this work, surface molecularly imprinted polymers based on magnetic multi-walled carbon nanotubes were prepared for the specific recognition and adsorption of resveratrol. The functionalization of magnetic multi-walled carbon nanotubes and the synthesis process of surface molecularly imprinted polymers were optimized. Characterizations were performed to demonstrate the successful synthesis of the imprinted materials. The imprinted materials showed satisfactory adsorption capacity of resveratrol (45.73 ± 1.72 mg/g) and excellent selectivity (imprinting factor 2.89 ± 0.15). In addition, the imprinted materials were used as adsorbents in molecularly imprinted solid-phase extraction for the purification of resveratrol from crude extracts of some food and medicinal resources, achieving recoveries of 93.69%-95.53% with high purities of 88.37%-92.33%. Moreover, the purified products exhibited extremely strong free radical scavenging activity compared with crude extracts. Overall, this work provided a promising approach for the highly selective purification of resveratrol from natural resources, which would contribute to the application of this valuable compound in the food/nutraceutical fields.

Genome-wide identification and expression analysis of MYB gene family in Cajanus cajan and CcMYB107 improves plant drought tolerance.

MYB transcription factor (TF) is one of the largest superfamilies that play a vital role in multiple plant biological processes. However, the MYB family has not been comprehensively identified and functionally verified in Cajanus cajan, which is the sixth most important legume crop. Here, 170 CcR2R3-MYBs were identified and divided into 43 functional subgroups. Segmental and tandem duplications and alternative splicing events were found and promoted the expansion of the CcR2R3-MYB gene family. Functional prediction results showed that CcR2R3-MYBs were mainly involved in secondary metabolism, cell fate and identity, developmental processes, and responses to abiotic stress. Cis-acting element analysis of promoters revealed that stress response elements were widespread in the above four functional branches, further suggesting CcR2R3-MYBs were extensively involved in abiotic stress response. The transcriptome data and qRT-PCR results indicated that most of the CcR2R3-MYB genes responded to various stresses, of which the expression of CcMYB107 was significantly induced by drought stress. Overexpression of CcMYB107 enhanced antioxidant enzyme activity and increased proline and lignin accumulation, thus improving the drought resistance of C. cajan. Furthermore, Overexpression of CcMYB107 up-regulated the expression of stress-related genes and lignin biosynthesis genes after drought stress. Our findings established a strong foundation for the investigation of biological function of CcR2R3-MYB TFs in C. cajan.

A novel three-dimensional morphological analysis of idiopathic condylar resorption following stabilisation splint treatment.

Bone modelling evaluation is important for monitoring idiopathic condylar resorption (ICR) progress. To compare condylar modelling in ICR patients treated with or without stabilisation splints (SSs). Eighty-four condyles from 84 ICR patients were studied: 42 received SS therapy (SS group); 42 received conventional therapy (control group). Cone-beam computed tomography images at diagnosis (T0) and after at least 6 months (T1) were used for three-dimensional reconstruction. Volume differences between T0 and T1 (δV) were used to evaluate the amount of modelling. Percentage of growth area (PCT) was used to assess the condylar surface growth tendency. No significant change in condylar volume was found in the SS group, whereas that in the control group was significantly decreased at T1 (P <.0001). The amount of modelling differed among condylar subregions within the SS group: among 6 subregions (P =.0137), between anterior and posterior regions (P =.0336) and between lateral, intermediate and medial regions (P =.0275). Control group condylar subregions showed no significant differences in the amount of modelling. The anabolic modelling tendency of the total condylar surface in the SS group was greater than that in the control group (P =.0251); however, there were no statistical differences in PCTs among condylar subregions in either group. SS therapy effectively reduced further bone destruction and promoted condylar modelling. Three-dimensional morphological analysis is a novel method that can accurately evaluate the amount of bone modelling and growth tendency in ICR patients.

A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies.

Porcine circovirus type 2 (PCV2) is the etiological agent of postweaning multisystemic wasting syndrome, a disease that causes huge economic damage in swine industry. A recombinant PCV2 expressing the neutralizing VP1 epitope (aa 141-160) of the foot-and-mouth disease virus (FMDV) was rescued using an infectious cloning technique. The PCV2 antigen and FMDV-VP1 antigenic epitope of the cloned strain recPCV2-CL-VP1 were confirmed by an immunoperoxidase monolayer assay (IPMA) and a capture enzyme-linked immunosorbent assay (ELISA). The morphological features of the recPCV2-CL-VP1 were not discernibly different from those of its parental strain (PCV2-CL). However, the recombinant virus could be differentiated from its parental virus by PCR and capture ELISA. The recPCV2-CL-VP1 was demonstrated to replicate stably in PK-15 cells through ten passages. An infection experiment using BALB/c mice showed that both recPCV2-CL-VP1 and PCV2-CL could replicate in the mice, cause various pathological changes, and induce a high level of anti-Cap antibodies. The recombinant virus emulsified with Freund's adjuvant was used to immunize BALB/c mice and induced antibodies against the FMDV-VP1 epitope. Hence, the recombinant PCV2 strain, which expressed the neutralizing FMDV-VP1 epitope, provides a valuable platform to develop novel genetic vaccines.

Atrial-esophageal fistula after atrial fibrillation ablation: a case report and literature review.

Background: Atrial-esophageal fistula (AEF) is a rare, but high mortality, complication after catheter ablation. At present, there is no standard treatment for AEF. In this article, we introduce the treatment process of a case diagnosed with AEF and review the latest treatment progress of AEF. Case Description: A 65-year-old man, who received catheter ablation 2 weeks prior, presented with fever, chills, and loss of consciousness. Blood cultures grew Streptococcus viridans. A computed tomography (CT) scan of the brain showed a large area of left craniocerebral infarction and air emboli in the right lobe. The chest CT demonstrated air between the left atrium and esophagus, as well as pericardial effusions. Gastroscopy showed an esophageal fistula 35 cm away from the incisor teeth. The patient was diagnosed with AEF, sepsis, and cerebral infarction. An urgent surgical operation and supportive treatments were performed after diagnosis. Eventually, he died of sepsis and multiple organ failure 24 days after surgery. Conclusions: We have reported the treatment process of one case diagnosed with AEF and reviewed the latest treatment progress. AEF is a rare but lethal complication after catheter ablation. At present, austere challenges exist in the diagnosis and treatment of AEF. Repeat chest and head CT/magnetic resonance imaging (MRI) are essential for the identification of abnormal manifestations. In terms of treatment, urgent surgical repair is currently recommended once AEF is diagnosed. More attention should be paid to this complication.

A nanocellulose molecularly imprinted membrane: Preparation, characterization and application in targeted separation of taxane 10-deacetylbaccatin III.

Targeted separation of active phytochemicals is urgently needed in the natural medicine field. In this paper, due to the natural porosity and high biocompatibility of cellulose, a nanocellulose membrane combined with surface molecular imprinting was successfully prepared; the efficient nanocellulose-based molecular imprinted membrane (NC-MIM) provided good adsorption for the targeted separation of phytochemicals such as 10-deacetylbaccatin III (10-DAB), an essential intermediate in the synthesis of the anticancer drug paclitaxel. Through a series of characterization and adsorption experiments, the adsorption mechanism of NC-MIM was determined. At pH 8.0 and temperatures of 20 °C-40 °C, the maximum capacity of NC-MIM for adsorption of 10-DAB reached 66.90 mg g - 1, and the content of 10-DAB was dramatically increased 17.5-fold after adsorption. The specific adsorption results showed that NC-MIM had excellent capacity for targeted separation of 10-DAB from among taxane structural analogues. Even after ten cycles, NC-MIM demonstrated a remarkable adsorption capacity of 86.43%, thereby indicating exceptional selectivity and stability. The successful implementation of NC-MIM for green, safe, and efficient enrichment of phytochemicals from plants provides a promising new approach and valuable insights into its practical application.

A cellulose-based intelligent temperature-sensitive molecularly imprinted aerogel reactor for specific recognition and enrichment of ursolic acid.

In this article, thermosensitive molecularly imprinted polymer and composite aerogel were combined for the first time to create an intelligent temperature-responsive aerogel reactor to effectively enrich ursolic acid (UA). Because aerogel carrier had a higher specific surface area and higher porosity compared to other carriers, the ursolic acid molecularly imprinted intelligent temperature responsive aerogel reactor (ITR&AR(G570)&UA-MIP) demonstrated a higher adsorption capacity for UA. More notably, ITR&AR(G570)&UA-MIP have the extraordinary capacity to spontaneously adsorb-desorb target molecule UA by regulating the reaction temperature. The ratio of the target molecule UA to the functional monomer and crosslinker in the grafting process and external influences had a major impact on how ITR&AR(G570)&UA-MIP were prepared overall. When the molar ratio of UA to 4-VP was 1:8, the weight ratio between ITR&AR(G570)&UA-MIP and EGDMA/DVB was 1:2:10, the reaction temperature was 60 °C, and the ambient pH = 6, the material showed the best adsorption capacity, reaching a peak of about 70 mg g-1. After researching the appropriate synthesis conditions, ITR&AR(G570)&UA-MIP were applied to lingonberry (Vaccinium Vitis-Idaea L.) berry extracts in this work. The outcomes show that this technique provides a new, intelligent, temperature-controlled adsorption material for the solid-phase extraction of triterpenoid acids in natural products, with good specific adsorption performance for the target molecule UA.

A novel ternary deep eutectic solvent pretreatment for the efficient separation and conversion of high-quality gutta-percha, value-added lignin and monosaccharide from Eucommia ulmoides seed shells.

A novel ternary deep eutectic solvent (DES), consisted of choline chloride, oxalic acid and ethylene glycol, was developed as a green, low-cost and recyclable pretreatment system for multi-stage utilization of Eucommia ulmoides seed shells. Under optimum conditions, 79.7 % hemicellulose and 65.6 % lignin were quickly removed while 84.0 % cellulose was retained. After DES pretreatment, the yield and purity of gutta-percha achieved 85.1 mg/g and 96.2 %, which increased 1.4 and 1.8 folds higher than that of un-treatment ones. Meanwhile, 69.1 % enzymatic digestibility of cellulose was obtained, that was 2.3 folds higher than that of raw substrates. Moreover, 53.6 % low-condensation lignin with aromatic structures and valuable aryl-ether linkages was well collected. Importantly, the DES that has been recycled five runs can still remove 73.9 % hemicellulose and 58.0 % lignin. Overall, the DES was determined to efficiently promote the separation and conversion of high-quality gutta-percha, value-added lignin and high-yield glucose from Eucommia ulmoides seed shells.

An all-cellulose sponge with a nanofiller-assisted hierarchical cellular structure for fruit maintaining freshness.

Cellulose sponges with compressibility and resilience are an ideal packaging material for fruits with fragile skin. Here, a soft and elastic all-cellulose sponge (CS) with a hierarchical cellular structure was fabricated, where the long molecular chain cellulose constructed major pores, the cellulose at nanoscale acted as an elastic nanofiller to fill the gaps of long molecular chain cellulose fibers and constructed minor pores. With these two kinds of pores, this structure can absorb strain hierarchically. The sponge can protect fruits from mechanical damage when dropped or repeated vibration. Furthermore, the CS modified with chlorogenic acid (C-CGAS) had excellent antibacterial and antifungal abilities. Therefore, C-CGAS could extend the storage time of strawberries to 18 days without any microbial invasion, which is the longest storage time reported thus far. This study provides a new idea for the preparation of polymer sponges and a new design for the development of antimicrobial packaging materials.

A Comparison of Volume of Tissue Removed and Biomechanical Analysis of Different Access Cavity Designs in 2-rooted Mandibular First Molars: A Multisample 3-dimensional Finite Element Analysis.

INTRODUCTION: The aim of this study was to compare the biomechanical properties and the amount of coronal tissue removed among the different access cavities with a multisample 3-dimensional finite element analysis in the mandibular first molar. The correlation between the amount of tissue removed and the fracture resistance of the teeth was also analyzed. METHODS: Micro-computed tomography data from 20 2-rooted mandibular first molars were included in this study as 3-dimensional modeling prototypes. Models of untreated molars and molars treated with the traditional access cavity (TradAC), the conservative access cavity (ConsAC), and the straight-line minimally invasive endodontic access cavities (SMIAC) were created. Each model was loaded in 3 ways to simulate the functional conditions of occlusion. The amount of tissue removed and the maximum stress in the cervical region were recorded and analyzed, and the correlation between them was also analyzed. RESULTS: The amount of coronal tissue and pericervical dentin (PCD) removed in SMIAC and ConsAC was less than that of TradAC. The mean maximum stress in the cervical region was significantly smaller in SMIAC and ConsAC than in TradAC. The amount of hard tissue and PCD loss was positively correlated with the maximum stress in the cervical region of the tooth. CONCLUSIONS: In mandibular first molars, it could be beneficial to improve the fracture resistance of the tooth after endodontic treatment by the minimally invasive access cavity to reduce the loss of coronal tooth tissue and PCD. The SMIAC may be an option balancing biomechanical properties and clinical convenience.

Three-dimensional analysis of coronal root canal morphology of 136 permanent mandibular first molars by micro-computed tomography.

BACKGROUND/PURPOSE: Minimally invasive endodontic approach become a research hotspot and may prevent the fracture of endodontically-treated teeth. This research aims to measure the coronal root canal morphology of permanent mandibular first molars in 3D and propose a new minimally invasive endodontic approach based on this measurement. MATERIALS AND METHODS: Data of 136 permanent mandibular first molars were involved and reconstructed in 3D models with canals. Then, the morphology characteristics of the coronal root canal were measured. RESULTS: Overall, the distribution of root canal orifices was more centralized than other landmarks. The landmarks were located more mesiobuccally to the center of the occlusal plane of molars. Specifically, the measurements of the maximum curvature of coronal root canals in the axial direction were: in 3-canals 2-rooted teeth, the average angles of curvatures were 23°,25°,11° for mesiobuccal (MB), mesiolingual (ML) and distobuccal (DB) canals, respectively; in 4-canals 2-rooted teeth were 23°,25°,12°,16°for MB, ML, DB, and distolingual (DL) canals, respectively; in 4-canals 3-rooted teeth were 25°,27°,17°,39° for MB, ML, DB, and DL canals, respectively. The degrees of coronal root canal curvatures in the horizontal direction were: in 3-canals teeth, the average angles of curvatures were -1°,47°,-2° for MB, ML and DB canals, respectively; in 4-canals 2-rooted teeth were -4°,41°,-25°,48° for MB, ML, DB, and DL canals, respectively; in 4-canals 3-rooted teeth were -3°,33°,-43°,79° for MB, ML, DB, and DL canals, respectively. CONCLUSION: The results of this study are similar to those previously obtained using CBCT and can help us design endodontic approaches.

[Segmentation and accuracy validation of mandibular molar and pulp cavity on cone-beam CT images by U-net neural network].

PURPOSE: To realize the automatic segmentation of mandibular molar and pulp cavity on cone-beam CT (CBCT) images by U-net convolutional neural network, and to use the 3D models reconstructed by Micro-CT data as the ground truth to validate its accuracy. METHODS： Twenty groups of small field of view(FOV) CBCT data containing complete mandibular molars were collected from the Department of Radiology, Affiliated Stomatology Hospital of Tongji University. After preprocessing, an endodontic specialist labeled teeth and pulp cavities by MITK Workbench software. These data were used as the training set for training U-net neural network. In addition, five mandibular molars and corresponding small FOV CBCT data were collected. These five CBCT were processed in the same way and used as the testing set. Then, teeth and pulp cavities on CBCT images of the testing set were segmented and reconstructed by U-net neural network and the same specialist. The isolated teeth were scanned by a Micro-CT machine after preprocessing and the results were reconstructed to 3D models, which were used as the ground truth. Then the 3D models reconstructed by the specialist's labeling, U-net network segmentation results, and the ground truth in the testing set were compared. Dice similarity coefficient(DSC), average symmetric surface distance (ASSD), Hausdorff distance (HD), and morphological analysis were used to evaluate the results. SPSS 20.0 software package was used for statistical analysis. RESULTS: Compared with the ground truth, the segmentation accuracy of the U-net neural network measured by DSC, ASSD, and AHD was (95.30±1.01)%, (0.11±0.02) mm, and (1.05±0.31) mm in teeth and (81.21±2.27)%, (0.15±0.05) mm, and (3.29±1.85) mm in the pulp cavity, respectively. Morphological analysis results showed that the U-net network segmentation results were similar to the ground truth in tooth and pulp chamber. As for the segmentation results of root canals, only thick root canals could be segmented rather than the thin root canals, such as the canals in the apical third and lateral root canals. CONCLUSIONS: Under the experimental conditions, the U-net neural network trained by the specialist's labeling realized the automatic and accurate segmentation of mandibular molar and their pulp chamber on CBCT images. For the segmentation of root canals, the results need to be further improved.

Minimally invasive treatment of calcified root canals in anterior teeth with digital guide technique.

OBJECTIVES: This study aims to establish a minimally invasive treatment using a customized digital template and a miniaturized bur for pulp canal obliteration (PCO). METHODS: Cone-beam computed tomography (CBCT) and digital oral scans of patients diagnosed with PCO in anterior teeth were obtained. Root canal morphology was reconstructed to accurately show the location, length, and direction of obliteration. A digital template was designed and fabricated using visual design software and 3D printing technology and used as guide for the miniaturized bur and drill sleeve during calcified tissue removal. A conventional root canal treatment was performed after the lumen of the root canal was reached. RESULTS: In both cases, the planned access cavity and glide paths were successfully established. In case 1, the deviated angle of the access cavity was 1.37°±0.07°, the deviation at the bottom of the miniaturized bur was 0.08-0.81 mm, the deviation at the tip of the bur was 0.05-1.13 mm, and difference in substance loss was 0.84-4.25 mm³. In case 2, the deviated angle of the access cavity was 3.09° ± 0.12°, the deviation at the bottom of the bur was 0.09-0.68 mm, the deviation at the tip of the bur was 0.29-0.66 mm, and the difference in substance loss was 0.55-3.79 mm3. CONCLUSIONS: Micro-guided endodontics is a novel approach for localizing and negotiating obliterated root canals and guarantees long-term prognosis without requiring excessive hard tissue removal.

Soft and elastic silver nanoparticle-cellulose sponge as fresh-keeping packaging to protect strawberries from physical damage and microbial invasion.

Strawberry is a nutritious food that is susceptible to mechanical injury and microbiological infection. Traditional coatings for strawberry packaging provide resistance against microbial infection but not against mechanical damage. In this study, a soft and elastic cellulose sponge modified with silver nanoparticles (AgNPs@CS-1:1) was prepared as strawberry packaging material, and it provided effective protection against mechanical damage. In addition, after 1000 cyclic compression, AgNPs@CS-1:1 presented only 16.80% unrecoverable deformation and still had elasticity, suggesting its fatigue resistance and durable protection for strawberry against damage caused by repeated vibrations during transportation. In addition, AgNPs@CS-1:1 had good antibacterial (E. coli and S. aureus) and antifungal (Rhizopus stolonifer) abilities. The storage time of strawberries packaged by AgNPs@CS-1:1 was extended to 12 days without microbial invasion. Thus, AgNPs@CS-1:1 provided dual protection at the physical and microbial levels. This study proposes a new method for the preservation of strawberries based on the utilization of cellulose.

Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports.

BACKGROUND: A palatal radicular groove is an unusual developmental deformity of the tooth, which may serve as a channel linking the periodontal and periapical inflammation, and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves. CASE SUMMARY: Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work. Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery. Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves, and 1162 operational taxonomic units were obtained. The phyla of Firmicutes and Proteobacteria predominated in most of the samples. An unknown genus from the Bacillaceae family, Lactococcus, and Porphyromonas were the most abundant genera identified. There was no difference in the microbiota richness and diversity in three sections of the groove. CONCLUSION: The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections. The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.

Micro-Computed Tomography-Guided Artificial Intelligence for Pulp Cavity and Tooth Segmentation on Cone-beam Computed Tomography.

INTRODUCTION: This study proposes a novel data pipeline based on micro-computed tomographic (micro-CT) data for training the U-Net network to realize the automatic and accurate segmentation of the pulp cavity and tooth on cone-beam computed tomographic (CBCT) images. METHODS: We collected CBCT data and micro-CT data of 30 teeth. CBCT data were processed and transformed into small field of view and high-resolution CBCT images of each tooth. Twenty-five sets were randomly assigned to the training set and the remaining 5 sets to the test set. We used 2 data pipelines for U-Net network training: one manually labeled by an endodontic specialist as the control group and one processed from the micro-CT data as the experimental group. The 3-dimensional models constructed using micro-CT data in the test set were taken as the ground truth. The Dice similarity coefficient, precision rate, recall rate, average symmetric surface distance, Hausdorff distance, and morphologic analysis were used for performance evaluation. RESULTS: The segmentation accuracy of the experimental group measured by the Dice similarity coefficient, precision rate, recall rate, average symmetric surface distance, and Hausdorff distance were 96.20% ± 0.58%, 97.31% ± 0.38%, 95.11% ± 0.97%, 0.09 ± 0.01 mm, and 1.54 ± 0.51 mm in the tooth and 86.75% ± 2.42%, 84.45% ± 7.77%, 89.94% ± 4.56%, 0.08 ± 0.02 mm, and 1.99 ± 0.67 mm in the pulp cavity, respectively, which were better than the control group. Morphologic analysis suggested the segmentation results of the experimental group were better than those of the control group. CONCLUSIONS: This study proposed an automatic and accurate approach for tooth and pulp cavity segmentation on CBCT images, which can be applied in research and clinical tasks.

A porous cellulose-based molecular imprinted polymer for specific recognition and enrichment of resveratrol.

A novel resveratrol molecularly imprinted polymer (p-CM@MPS@MIP-Res) was prepared on the surface of silanized porous cellulose microspheres (p-CM@MPS) for the first time, and was successfully applied for the efficient enrichment of targeted resveratrol in Polygonum cuspidatum. The adsorption kinetics and adsorption equilibrium of p-CM@MPS@MIP-Res were also studied in detail. Compared with non-molecularly imprinted polymer (p-CM@MPS@NIP), the prepared p-CM@MPS@MIP-Res showed high adsorption capacity for resveratrol, the adsorption capacity of the p-CM@MPS@MIP-Res could reach to 11.56 mg/g. Furthermore, the stability of the p-CM@MPS@MIP-Res was evaluated and the result showed that the p-CM@MPS@MIP-Res could be reused for 5 runs. Finally, the p-CM@MPS@MIP-Res was applied to enrich the resveratrol in Polygonum cuspidatum sample, the content of resveratrol in the extraction solution could be increased greatly from 4.23 % to 23.74 %, indicating the p-CM@MPS@MIP-Res was a promising adsorbent for efficiently separation and enrichment of resveratrol in Polygonum cuspidatum.

A cellulose-based temperature sensitivity molecular imprinted hydrogel for specific recognition and enrichment of paclitaxel.

A microcrystalline cellulose-based temperature sensitivity paclitaxel molecular imprinted hydrogel (MCC-TSMIHs-PTX) was successfully prepared by temperature-sensitive monomer N-isopropylacrylamide, functional monomer 4-vinylpyridine, cross-linking agent N, N'-methylenebisacrylamide and microcrystalline cellulose. They showed imprinting effective responses to the temperature changes. The results of adsorption kinetics, adsorption equilibrium, thermodynamics, selectivity and reusability showed the successful formation of a grafting thermosensitivity hydrogel with higher adsorption capacity and specific recognition. When the temperature reached 308 K, imprinting effect of hydrogel cavities would be most effective and conducive to capture template molecules. When the temperature reached 288 K, the lowest imprinting effect would facilitate the desorption of PTX. Finally, the MCC-TSMIHs-PTX was applied to enrich the paclitaxel in Taxus × media extracts samples, the relative contents of PTX in the samples were increased greatly from 7.23% to 78.32%, indicating the MCC-TSMIHs-PTX was a stable adsorption capacity for efficient separation and enrichment of PTX in Taxus × media extracts.

Magnetism and NIR dual-response polypyrrole-coated Fe3O4 nanoparticles for bacteria removal and inactivation.

Microbial contamination of water represents a great threat to the public health that has attracted worldwide attention. In this work, polypyrrole magnetic nanoparticles (Fe3O4@PPy NPs) with sterilization properties were fabricated. More specifically, the Fe3O4@PPy NPs obtained via aqueous dispersion polymerization and an in situ chemical oxidative polymerization exhibited a cationic surface and high photothermal conversion efficiency. More than 50% of bacteria adsorption can be achieved at a dosage of 100 µg/mL Fe3O4@PPy NPs under magnetic field, and high photothermal sterilization efficacy (~100%) can be obtained upon NIR exposure at the same dosage for 10 min. Noteworthy, the Fe3O4@PPy NPs can be recycled by magnetism and reused without affecting their photothermal sterilization capability. This study clearly provides experimental evidence of the great potential of Fe3O4@PPy NPs as stable and reusable nanocomposite materials for bacteria adsorption and photothermal sterilization performance. The application of Fe3O4@PPy NPs can realize enviromental-friendly bacterial contaminated water treatment as well as provide stratgies for synergistical antibacterial materials design.

Preparation and application of modified three-dimensional cellulose microspheres for paclitaxel targeted separation.

In this article, we successfully prepared three-dimensional cellulose microspheres modified by molecularly imprinted polymer for paclitaxel recognition and separation (3D-CM &PTX&MIPs). The material was characterized by Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TG) and diffraction of X-rays (XRD). Under the optimized adsorption conditions, the maximum adsorption capacity reached 65.7 mg/g. And after 5 runs of reuse, (3D-CM&PTX&MIPs) still maintained a reusability rate of 90%. Besides, (3D-CM&PTX&MIPs) showed excellent selectivity for target PTX. Finally, (3D-CM&PTX&MIPs) was used for PTX recognition and separation in the extracts of yew leaves. This research laid a good foundation and scientific basis for the efficient, environmentally friendly, and rapid enrichment of metabolites in plants using bio-based molecularly imprinted polymers.