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1.
Urol Int ; 88(1): 60-5, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22222954

RESUMO

OBJECTIVE: To investigate whether the peritoneal cavity could function as a bioreactor to produce autologous tubular grafts for ureteral reconstruction in beagles. MATERIALS AND METHODS: 8-Fr Silastic tubes were implanted into the peritoneal cavities of 6 female beagles. At 3 weeks, the tubes were harvested and the tubular tissue covering the tubes was gently everted. A segment 3 cm in length of the right mid-ureter, involving two thirds of its diameter, was removed parallel to the ureteral axis, leaving a third of the ureteral wall. A 5-Fr double-J stent was inserted into the ureter through the created defect, and two thirds of the graft were anastomosed to both edges of the ureteral defect. One third of the graft was overlapped with the retained normal ureter and anastomosed to the external surface of the lumens. Thus, the graft was partly encapsulated by the remainder of ureteral wall. The stent was maintained for 6 weeks and removed. Excretory urography was performed at 8 (n = 3) and 12 weeks (n = 3), postoperatively. Meanwhile, the neoureter was harvested and analyzed. The left ureter served as the control and a simple intubated ureterotomy was performed. RESULTS: Histological analysis of the tubular tissue demonstrated transversely arranged myofibroblasts and an outer layer of mesothelium. The tissue was easily everted and transplanted as a ureteral graft. Eight weeks postoperatively, the neoureter demonstrated normal ureteral architecture, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. Excretory urography indicated no stenosis or hydronephrosis. CONCLUSIONS: These results show that autologous tubular tissue grown within the recipients' peritoneal cavity can be used for ureteral reconstruction in the beagle model.


Assuntos
Miofibroblastos/transplante , Engenharia Tecidual/métodos , Alicerces Teciduais , Ureter/cirurgia , Obstrução Ureteral/cirurgia , Urotélio/transplante , Anastomose Cirúrgica , Animais , Reatores Biológicos , Diferenciação Celular , Proliferação de Células , Constrição Patológica , Dimetilpolisiloxanos , Modelos Animais de Doenças , Cães , Desenho de Equipamento , Feminino , Cavidade Peritoneal/cirurgia , Radiografia , Stents , Fatores de Tempo , Transplante Autólogo , Ureter/diagnóstico por imagem , Ureter/patologia , Obstrução Ureteral/diagnóstico por imagem , Obstrução Ureteral/patologia
2.
World J Urol ; 28(2): 227-32, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19588153

RESUMO

PURPOSE: To investigate whether peritoneal cavity could function as bioreactor to produce autologous tubular grafts for urethral reconstruction in male rabbits. METHODS: 8Fr silastic tubes were implanted into peritoneal cavities of nine male rabbits. By 2 weeks, tubes were harvested and the tubular tissue covering the tubes was everted. A pendulous urethral segment of 1.5 cm long was totally excised and urethroplasty was performed with the everted tubular tissue in an end-to-end fashion. Another nine male rabbits underwent the same urethral resection and re-anastomosis as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neo-urethra were harvested and analyzed grossly and histologically. RESULTS: Histological analysis of the tubular tissue demonstrated transversely arranged myofibroblasts embedded in homogeneous collagen bundles and an outer layer of mesothelium. The tissue was easily everted and successfully transplanted as a urethral graft. Serial urethrography indicated no stricture or diverticula formation. While all animals of the control group developed stricture. Histological analysis of the neo-urethra demonstrated normal urethral architecture by 1 month, composed of multi-layers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neo-urethra could be hardly distinguished from native urethra both grossly and histologically. CONCLUSIONS: These results show that the autologous tissue grown within the recipients' peritoneal cavity can be used successfully for tubularized urethral reconstruction in male rabbits.


Assuntos
Reatores Biológicos , Cavidade Peritoneal , Engenharia Tecidual/métodos , Uretra/citologia , Uretra/transplante , Animais , Dimetilpolisiloxanos , Epitélio , Masculino , Modelos Animais , Coelhos , Procedimentos de Cirurgia Plástica , Coleta de Tecidos e Órgãos , Transplante Autólogo , Uretra/crescimento & desenvolvimento
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