RESUMO
We report on a 3-year-old boy with moderate developmental retardation, microcephaly, and malformations of ears, lids, mouth, and thumbs. Cytogenetic analysis demonstrated a direct duplication of chromosome subregion 4(q21.3-->q31.3). Confirmation of this specific rearrangement was performed by fluorescent in situ hybridization (FISH) with a chromosome painting probe and by means of quantitative Southern hybridization with DNA probes localized within the chromosome 4 region presumed to be duplicated.
Assuntos
Anormalidades Múltiplas/genética , Aberrações Cromossômicas/genética , Cromossomos Humanos Par 4 , Deficiências do Desenvolvimento/genética , Microcefalia/genética , Adulto , Southern Blotting , Pré-Escolar , DNA/análise , Face/anormalidades , Ossos Faciais/anormalidades , Feminino , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Masculino , Polegar/anormalidadesRESUMO
The release characteristics of potassium chloride was studied when it was coated with a selection of polymers; from the results obtained, a suitable batch was microencapsulated using a gelatin-gum arabic coacervate system. The microencapsulated product offers better controlled release for this drug when compared to standard table and powder forms.
Assuntos
Cápsulas , Cloreto de Potássio/administração & dosagem , Preparações de Ação Retardada , Polímeros , Cloreto de Potássio/metabolismo , Solubilidade , Fatores de TempoRESUMO
The Coulter counter has become one of the methods of choice for the measurement of small particle sizes. However, many colloidal walled microcapsules are prone to hydration when dispersed in the electrolyte solution used in Coulter measurements. This hydration causes the walls to swell, producing different size analyses dependent upon the time of measurement. In the present work the change in size with time was studied for microcapsules with gelatin-acacia coacervate, or ethylcellulose, walls. The former, although rendered insoluble with formalin, still hydrated; the latter were almost unaffected by water. A sample size of 30 mg was required to prevent blocking of the orifice with larger samples, or too few particles to permit measurement in smaller samples. The coacervate coated microcapsules showed a bimodal distribution, in part due to aggregate of smaller microcapsules, and this distribution changed significantly over the time of measurement. Although the peak due to larger microcapsules did not disappear, the number of large microcapsules fell significantly, most probably because of the disintegration of aggregated microcapsules. Microcapsules with ethyl cellulose walls did not show a bimodal distribution and the particle size analysis did not alter significantly with time.