Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers.

Specific recognition and highly sensitive detection of biomarkers play an essential role in identification, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIPs) have been widely used to capture biomimetic receptors for targets in various complex matrices due to their superior recognition ability, structural stability, and rapid separation characteristics, which overcome the existing deficiencies of traditional recognition elements such as antibodies, aptamers. The integration of MMIPs as recognition elements with chemical sensors opens new opportunities for the development of advanced analytical devices with improved selectivity and sensitivity, shorter analysis time, and lower cost. Recently, MMIPs-chemical sensors (MMIPs-CS) have made significant progress in detection, but many challenges and development spaces remain. Therefore, this review focuses on the research progress of the sensor based on biomarker detection and introduces the surface modification of the magnetic support material used to prepare high selective MMIPs, as well as the selective extraction of target biomarkers by MMIPs from the complex biological sample matrix. Based on the understanding of optical sensors and electrochemical sensors, the applications of MMIPs-optical sensors (MMIPs-OS) and MMIPs-electrochemical sensors (MMIPs-ECS) for biomarker detection were reviewed and discussed in detail. Moreover, it provides an overview of the challenges in this research area and the potential strategies for the rational design of high-performance MMIPs-CS, accelerating the development of multifunctional MMIPs-CS.

SERS-based boronate affinity biosensor with biomimetic specificity and versatility: Surface-imprinted magnetic polymers as recognition elements to detect glycoproteins.

Glycoproteins are a class of proteins with significant biological functions and clinical implications. Due to glycoproteins' reliability for the quantitative analysis, they have been used as biomarkers and therapeutic targets for disease diagnosis. We propose a sandwich structure-based boronate affinity biosensor that can separate and detect target glycoproteins by magnetic separation and Surface-enhanced Raman scattering (SERS) probes. The biosensor relies on boronic acid affinity magnetic molecularly imprinted polymer (MMIPs) with pH response as "capturing probe" for glycoproteins, and Au-MPBA@Ag modified with 4-mercaptophenylboronic acid (MPBA) as SERS probes, among which, MPBA has both strong SERS activity and can specifically recognize and bind to glycoproteins. MMIPs ensured specific and rapid analysis, and SERS detection provided high sensitivity. The proposed boronate affinity SERS strategy exhibited universal applicability and provided high sensitivity with limit of detection of 0.053 ng/mL and 0.078 ng/mL for horseradish peroxidase and acid phosphatase, respectively. Ultimately, the boronate affinity SERS strategy was successfully applied in detection of glycoprotein in spiked serum sample with recovery between 90.6% and 103.4%, respectively. In addition, this study used a portable Raman meter, which can meet the requirements of point-of-care testing. The biosensor presented here also has advantages in terms of cost-effectiveness, stability, and detection speed.