[Progress in antibacterial coatings of titanium implants surfaces].

In recent years, bone implant materials such as titanium and titanium alloys have been widely used in the biomedical field due to their excellent mechanical properties and good biocompatibility. However, in clinical practice, bacterial adhesion to the material surface and postoperative infection issues may lead to implantation failure. Based on the antibacterial mechanism, this review elaborated on the antibacterial surface design of titanium implants from the aspects of anti-bacterial adhesion, contact sterilization and photocontrol sterilization. Surface modification of titanium or titanium-based alloy implants with different techniques can inhibit bacteria and promote osseointegration. Thus, the application range of multifunctional titanium-based implants in the field of orthopedics will be expanded.

[Progress in preparation and application of sodium alginate microspheres].

Sodium alginate (SA) is a kind of natural polymer material extracted from kelp, which has excellent biocompatibility, non-toxicity, biodegradability and abundant storage capacity. The formation condition of sodium alginate gel is mild, effectively avoiding the inactivation of active substances. After a variety of preparation methods, sodium alginate microspheres are widely used in the fields of biomaterials and tissue engineering. This paper reviewed the common methods of preparing alginate microspheres, including extrusion, emulsification, electrostatic spraying, spray drying and coaxial airflow, and discussed their applications in biomedical fields such as bone repair, hemostasis and drug delivery.

Water-triggered shape memory cellulose / sodium alginate / montmorillonite composite sponges for rapid hemostasis.

Uncontrollable bleeding caused by severe trauma is life-threatening. Therefore, it is of great significance to develop hemostatic materials that meet the rapid hemostasis of wounds. In this study, a water-triggered shape memory carboxylated cellulose nanofiber/sodium alginate/montmorillonite (CNSAMMTCa) composite hemostatic sponge was prepared, which can promote coagulation by concentrating the blood and activating intrinsic pathway. The anisotropic three-dimensional porous structure formed by directional freeze-drying technology improved the performance of composite sponges which showed good prospects in rapid hemostasis. The results showed that CNSAMMTCa composite sponge had good porous structure, water absorption ability, cytocompatibility and blood cell aggregation capacity. Simultaneously, we confirmed that CNSA3MMT2Ca has best coagulation performance in the mouse censored bleeding model and liver rupture bleeding model. Therefore, CNSAMMTCa composite hemostatic sponge is a safe and efficient rapid hemostatic material which is expected to become an alternative material for clinical hemostatic materials.

Sodium alginate/carboxycellulose/polydopamine composite microspheres for rapid hemostasis of deep irregular wounds.

Hemostasis of deep irregular wounds is a severe problem in clinical practice. The development of rapid-acting hemostatic agents for deep and irregular wound is urgently needed. Here, sodium alginate/carboxycellulose/polydopamine (SA/CNF/PDA) microspheres was prepared by reverse emulsification and crosslinking with Ca2+, and SA/CNF/PDA composite hemostatic microspheres with porous structure were obtained by freeze-drying. SA/CNF/PDA composite hemostatic microspheres exhibited excellent porosity and water absorption which could rapidly absorb blood on the wound surface. Moreover, SA/CNF/PDA composite microspheres demonstrated remarkable hemostatic capabilities both in vitro and in vivo. It exhibited strong hemostatic performance in models of mouse tail-break and liver damage. Especially in liver injury model, it was completely hemostatic in 95 s, and blood loss (19.3 mg). The hemostatic efficacy of the SA/CNF/PDA composite microspheres was amplified through the stimulation of both exogenous and endogenous coagulation pathways. Therefore, SA/CNF/PDA composite hemostatic microspheres are suitable for rapid hemostasis of deep irregular wounds which are potential rapid hemostatic material for surgical application.

Hydroxyapatite/Polyurethane Scaffolds for Bone Tissue Engineering.

Polyurethane (PU) and PU ceramic scaffolds are the principal materials investigated for developing synthetic bone materials due to their excellent biocompatibility and biodegradability. PU has been combined with calcium phosphate (such as hydroxyapatite [HA] and tricalcium phosphate) to prepare scaffolds with enhanced mechanical properties and biocompatibility. This article reviews the latest progress in the design, synthesis, modification, and biological attributes of HA/PU scaffolds for bone tissue engineering. Diverse HA/PU scaffolds have been proposed and discussed in terms of their osteogenic, antimicrobial, biocompatibility, and bioactivities. The application progress of HA/PU scaffolds in bone tissue engineering is predominantly introduced, including bone repair, bone defect filling, drug delivery, and long-term implants.

Improving osseointegration and antimicrobial properties of titanium implants with black phosphorus nanosheets-hydroxyapatite composite coatings for vascularized bone regeneration.

For decades, titanium implants have shown impressive advantages in bone repair. However, the preparation of implants with excellent antimicrobial properties as well as better osseointegration ability remains difficult for clinical application. In this study, black phosphorus nanosheets (BPNSs) were doped into hydroxyapatite (HA) coatings using electrophoretic deposition. The coatings' surface morphology, roughness, water contact angle, photothermal properties, and antibacterial properties were investigated. The BP/HA coating exhibited a surface roughness of 59.1 nm, providing an ideal substrate for cell attachment and growth. The water contact angle on the BP/HA coating was measured to be approximately 8.55°, indicating its hydrophilic nature. The BPNSs demonstrated efficient photothermal conversion, with a temperature increase of 42.2°C under laser irradiation. The BP/HA composite coating exhibited a significant reduction in bacterial growth, with inhibition rates of 95.6% and 96.1% against Staphylococcus aureus and Escherichia coli. In addition, the cytocompatibility of the composite coating was evaluated by cell adhesion, CCK8 and AM/PI staining; the effect of the composite coating in promoting angiogenesis was assessed by scratch assay, transwell assay, and protein blotting; and the osteoinductivity of the composite coating was evaluated by alkaline phosphatase assay, alizarin red staining, and Western blot. The results showed that the BP/HA composite coating exhibited superior performance in promoting biological functions such as cell proliferation and adhesion, antibacterial activity, osteogenic differentiation, and angiogenesis, and had potential applications in vascularized bone regeneration.

Multilayer functional bionic fabricated polycaprolactone based fibrous membranes for osteochondral integrated repair.

Osteochondral defect repair is one of the challenging problems in orthopedics. In this study, a multilayer polycaprolactone (PCL) based fibrous membrane for osteochondral defect repair was biomimetically fabricated by combining self-induced crystallization, biomimetic mineralization and layer-by-layer electrospinning techniques. The multilayer functional bionic fibrous membrane consisted of cartilage repair layer, intermediate transition repair layer and subchondral bone repair layer. Glucosamine hydrochloride (GAH) encapsulated in core-shell structured PCL fibrous membrane (MGPCL) was suitable for cartilage repair. Shish-kebab (SK) structured PCL fibrous membrane with calcium phosphate coating (MSKPCL) was designed for subchondral bone repair. SK structured MGPCL fibrous membrane (SKMGPCL) was used as intermediate transition repair. The tensile modulus of MG/SKMG/MSKPCL fibrous membrane was 34.24 ± 2.39 MPa which met the requirements of cartilage and subchondral bone repair scaffolds, and in vitro culture results showed that MG/SKMG/MSKPCL fibrous membrane had good biological activity and osteogenic ability. These results showed that MG/SKMG/MSKPCL fibrous membrane provides a promising material basis for osteochondral integrated repair scaffold.

In situ titanium phosphate formation on a titanium implant as ultrahigh bonding with nano-hydroxyapatite coating for rapid osseointegration.

Titanium (Ti) has been widely used as a dental implant material due to its excellent mechanical property and good biocompatibility. However, its poor biological activity severely limits its ability to bond with bony tissues. To ameliorate this situation, a preparation method of ultra-high bonding nano-hydroxyapatite (n-HA) coating on the Ti surface is urgently needed. Here, Ti phosphate/n-HA (TiP-Ca) composite coatings with ultra-high bonding were prepared by a two-step hydrothermal treatment. The TiP coating was first formed in situ on the pure Ti substrate and then n-HA crystals further grew on the TiP surface. The formation mechanism of composite coating and reasons for increased bonding strength were systematically investigated. The results show that the TiP-Ca coating remains stable and exhibits an ultra-high bonding strength with the Ti implant (up to 783.30 ± 207.46 N). An effective solution was designed to address the problems of easy peel off. Cell experiments showed that TiP-Ca could promote the adhesion of MC3T3-E1 and expression of OCN, Runx2, and ALP. In vivo evaluation further confirmed that the TiP-Ca composite coating significantly enhanced osseointegration. The designed coating shows great potential in clinical application of implants.

Calcium Phosphate Bone Cements Incorporated with Black Phosphorus Nanosheets Enhanced Osteogenesis.

For decades, calcium phosphate bone cements (CPCs) showed impressive advantages for their good biocompatibility, injectability, and osteoconductivity in the bone repair field. However, it is still difficult to prepare CPCs with outstanding antibacterial and self-curing properties, sufficient phosphorus release, and osteoinductivity for clinical application. Herein, we used partially crystallized calcium phosphate and dicalcium phosphate anhydrate particles incorporated with black phosphorous nanosheets to prepare calcium phosphate bone cements (CPCs). The curing time, compressive strength, photothermal properties, and degradation performance of BP/CPC were investigated. In addition, the cytocompatibility and osteoinductivity of BP/CPC were evaluated by cell adhesion, cytotoxicity, alkaline phosphatase detection, alizarin red staining, and western blot assay. The results indicated that BP/CPC showed adjustable curing time, good cytocompatibility, outstanding photothermal properties, and osteoinductivity, suggesting their potential application for bone regeneration.

A facile method to fabricate high performance PVA/PAA-AS hydrogel via the synergy of multiple hydrogen bonding and Hofmeister effect.

Hydrogels are widely used in biomedical engineering, which often require matched mechanical properties to meet specific demands. Recently, numerous research studies have contributed to tissue engineering hydrogels by soaking strategies to obtain designed properties. Herein, a strategy to fabricate poly(vinyl alcohol)/poly(acrylic acid)-ammonium sulfate (PVA/PAA-AS) hydrogel by successively soaking an aqueous PAA solution and (NH4)2SO4 solution based on the synergy of multiple hydrogen bonding and Hofmeister effect is reported, which exhibits remarkable comprehensive mechanical properties: rigidity (elastic modulus: 0.7-3.6 MPa), strength at break (tensile stress: 3.2-12.0 MPa; strain 320-650%), and toughness (fracture energy: 4.5-30.0 MJ m-3). Besides, PVA/PAA-AS hydrogel with unique spring-like microstructure exhibited super-resilience in 30% strain range by energy-transforming mechanism. Compared with pure PVA hydrogel, PVA/PAA-AS hydrogel has the equal excellent cytocompatibility. Therefore, PVA/PAA-AS hydrogel with high strength, modulus, toughness, super-resilience and excellent biocompatibility has potential applications in the soft tissue engineering field such as muscles, tendons, and ligaments.

Abundant tannic acid modified gelatin/sodium alginate biocomposite hydrogels with high toughness, antifreezing, antioxidant and antibacterial properties.

The acidity of high tannic acid (TA) content solution can destroy the structure of protein, such as gelatin (G). This causes a big challenge to introduce abundant TA into the G-based hydrogels. Here, the G-based hydrogel system with abundant TA as hydrogen bonds provider was constructed by a "protective film" strategy. The protective film around the composite hydrogel was first formed by the chelation of sodium alginate (SA) and Ca2+. Subsequently, abundant TA and Ca2+ were successively introduced into the hydrogel system by immersing method. This strategy effectively protected the structure of the designed hydrogel. After treatment with 0.3 w/v TA and 0.06 w/v Ca2+ solutions, the tensile modulus, elongation at break and toughness of G/SA hydrogel increased about 4-, 2-, and 6-fold, respectively. Besides, G/SA-TA/Ca2+ hydrogels exhibited good water retention, anti-freezing, antioxidant, antibacterial properties and low hemolysis ratio. Cell experiments showed that G/SA-TA/Ca2+ hydrogels possessed good biocompatibility and could promote cell migration. Therefore, G/SA-TA/Ca2+ hydrogels are expected to be used in the field of biomedical engineering. The strategy proposed in this work also provides a new idea for improving the properties of other protein-based hydrogels.

The study of self-regulating α-TCP based composite by micro/nano scaled silk fibroin and α-CSH on physicochemical and biological properties of bone cement.

A novel self-hardening α-tricalcium phosphate (α-TCP) bone cement complexed with different content of α-calcium sulfate hemihydrate (α-CSH) and micrometer hydroxyapatite mineralized silk fibroin (HA-SF) using micro/SF as curing liquid has been investigated in this work, which was capable of tunable setting time, degradation, mechanical property and ability to anti-washout. After addition 0 ∼ 25% α-CSH to the α-TCP cement with SFFs as curing liquid, it shortened the setting time of the modified composite to 10 ∼ 30 min. Furthermore, the addition of SFFs improved the compressive strength of the composite from 5.41 MPa to 9.44 MPa. The composites with both Na2HPO4 and SFFs as curing liquid showed good anti-collapse performance. The weight loss ratio of bone cement was -0.18 ∼ 12.08% in 4 weeks when the content of α-CSH in α-TCP/α-CSH was between 0 ∼ 25 wt%. During the degradation of α-CSH, the amorphous α-TCP were deposited as hydroxyapatite to formed a plate-like products on the surface of composite. Compared to the composite with Na2HPO4 solution as the curing liquid, alkaline phosphatase (ALP) activity of the composites using SFFs as curing liquid were maintained at high levels on the 14th day especially when the Ca/P ratio was 1.7. This study provides a theoretical basis for the regeneration of bone defects guided by bone cement materials.

Cellulose based composite sponges with oriented porous structure and superabsorptive capacity for quick hemostasis.

Excessive bleeding is the leading cause of death in accidents and operations. Ca2+ crosslinked carboxyl nanocellulose (CN)/montmorillonite (MMT) composite (CaCNMMT) sponges were prepared by uniform mixing and directional freeze-drying methods which was inspired by the coordination mechanism of blood clot formation and coagulation cascade activation in natural hemostasis process. Carboxyl nanocellulose (CaCN) sponge has instantaneous water absorption capacity, and CaCNMMT sponges could further activate clotting factors. Therefore, CaCNMMT sponges achieved quick hemostasis by efficient concentrating blood, inducing hemocyte aggregation and stimulating coagulation cascade activation based on the synergistic effects of CN and MMT. Blood clotting index of CaCNMMT (15.90 ± 0.52 %) was significantly lower than CaCN (59.3 ± 1.43 %), and APTT time (22 ± 2 s) was almost equivalent to MMT (20 ± 2 s). CaCNMMT sponge showed good quick hemostatic effect on massive hemorrhage in both tail-breaking and liver injury model which provided a new strategy for the application of MMT in hemostatic and trauma treatment fields.

Bifunctional electrospun poly (L-lactic acid) membranes incorporating black phosphorus nanosheets and nano-zinc oxide for enhanced biocompatibility and antibacterial properties in catheter materials.

For several decades, urinary tract infections caused by catheter-associated devices have negatively impacted not only medical device utilization, but also patient health. As such, the creation of catheter materials with both superior biocompatibility and antibacterial properties has become necessary. This study aimed to produce electrospun membranes based on polylactic acid (PLA) with the incorporation of black phosphorus nanosheets (BPNS) and nano-zinc oxide (nZnO) particles, as well as a mixture of both, in order to design bifunctional membranes with enhanced bioactivity and antibacterial features. The optimum spinning process was determined through examination of various PLA mass concentrations, spinning solution propelling speeds, and receiving drum rotating speeds, with emphasis on the mechanical properties of PLA membranes. Additionally, the antibacterial properties and cytocompatibility of the ZnO-BP/PLA antibacterial membranes were explored. Results demonstrated that the ZnO-BP/PLA antibacterial membranes displayed a rich porous structure, with uniform distribution of nZnO particles and BPNS. With the increase of polylactic acid concentration and the decrease of spinning solution advancing and drum rotation speeds, the mechanical properties of the fiber membrane were significantly improved. Furthermore, the composite membranes exhibited remarkable photothermal therapy (PTT) capabilities when aided by the synergistic effect of BP nanosheets and ZnO. This was achieved through near-infrared (NIR) irradiation, which not only dissipated the biofilm but also enhanced the release capability of Zn2+. Consequently, the composite membrane demonstrated an improved inhibitory effect on both Escherichia coli and Staphylococcus aureus. The results of cytotoxicity and adhesion experiments also indicated good cytocompatibility, with cells growing normally on the surface of the ZnO-BP/PLA antibacterial membrane. Overall, these findings validate the utilization of both BPNS and n-ZnO fillers in the creation of novel bifunctional PLA-based membranes, which possess both biocompatibility and antibacterial properties for interventional catheter materials.

Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.

Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid. Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone. Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM. To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology. The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface. The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM. The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively. After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%. In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.

Core-Shell Nanofibers with a Shish-Kebab Structure Simulating Collagen Fibrils for Bone Tissue Engineering.

The repair of bone defects is one of the great challenges facing modern orthopedics clinics. Bone tissue engineering scaffold with a nanofibrous structure similar to the original microstructure of a bone is beneficial for bone tissue regeneration. Here, a core-shell nanofibrous membrane (MS), MS containing glucosamine (MS-GLU), MS with a shish-kebab (SK) structure (SKMS), and MS-GLU with a SK structure (SKMS-GLU) were prepared by micro-sol electrospinning technology and a self-induced crystallization method. The diameter of MS nanofibers was 50-900 nm. Contact angle experiments showed that the hydrophilicity of SKMS was moderate, and its contact angle was as low as 72°. SK and GLU have a synergistic effect on cell growth. GLU in the core of MS was demonstrated to obviously promote MC3T3-E1 cell proliferation. At the same time, the SK structure grown on MS-GLU nanofibers mimicked natural collagen fibers, which facilitated MC3T3-E1 cell adhesion and differentiation. This study showed that a biomimetic SKMS-GLU nanofibrous membrane was a promising tissue engineering scaffold for bone defect repair.

Electrospun polyamide-6/chitosan nanofibers reinforced nano-hydroxyapatite/polyamide-6 composite bilayered membranes for guided bone regeneration.

Periodontal defect poses a significant challenge in orthopedics. Guided Bone Regeneration (GBR) membrane is considered as one of the most successful methods applied to reconstruct alveolar bone and then to achieve periodontal defect repair/regeneration. In this paper, a novel polyamide-6/chitosan@nano-hydroxyapatite/polyamide-6 (PA6/CS@n-HA/PA6) bilayered tissue guided membranes by combining a solvent casting and an electrospinning technique was designed. The developed PA6/CS@n-HA/PA6 composites were characterized by a series of tests. The results show that n-HA/PA6 and electrospun PA6/CS layers are tightly bound by molecular interaction and chemical bonding, which enhances the bonding strength between two distinct layers. The porosity and adsorption average pore diameter of the PA6/CS@n-HA/PA6 membranes are 36.90 % and 22.61 nm, respectively. The tensile strength and elastic modulus of PA6/CS@n-HA/PA6 composites are 1.41 ± 0.18 MPa and 7.15 ± 1.09 MPa, respectively. In vitro cell culture studies demonstrate that PA6/CS@n-HA/PA6 bilayered scaffolds have biological safety, good bioactivity, biocompatibility and osteoconductivity.

Mineralized Polyamide66/Calcium Chloride Nanofibers for Bone Tissue Engineering.

This study aimed at designing a novel electrospun scaffolding material that structurally and chemically resembles native extracellular matrix for bone tissue engineering. Calcium chloride-complexed polyamide66 (PA66/CaCl2) and pure PA66 electrospun nanofibers were fabricated by the electrospinning method. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared (FTIR) spectroscopy were used to investigate the effect of the presence of ionized salt in the polymer solution on the mechanical properties and other properties of the electrospun scaffolds. The results show that addition of CaCl2 to PA66 solution can achieve an internal modification and improve the tensile strength and modulus of the polymeric electrospun nanofiber. Ca2+ uploaded through electrospun fibers could provide nucleation sites for the formation of hydroxylapatite (HA) coating. After mineralization, the tensile strength and modulus of HA/PA66/CaCl2 scaffolds reach up to 41.33 ± 16.17 MPa and 168.59 ± 42.20 MPa, respectively. LIVE/DEAD assay shows that compared with pure PA66 scaffolds, a greater density of viable MC3T3-E1 cells were seen on the HA/PA66/CaCl2 scaffolds. Cell Counting Kit-8 results indicate that HA/PA66/CaCl2 scaffolds displays a more favorable ability to promote MC3T3-E1 cell proliferation and growth than that of the other groups with the prolongation of culture time. These results demonstrate that HA/PA66/CaCl2 scaffolds that structurally and chemically resemble native bone have a good cytocompatibility, and might be a potential candidate for bone tissue engineering. [Figure: see text] Impact statement In this work, the calcium chloride-complexed polyamide66 (PA66/CaCl2) hybrid nanofibers were prepared by adding CaCl2 to the PA66 formic acid solution before electrospinning, and then mineralized by saturated calcium phosphate solution. The findings of this study show that the addition of CaCl2 could achieve an internal modification, improve the tensile strength and modulus of the polymeric electrospun nanofiber, and provide nucleation sites for biomimetic mineralization. This research provides insight and foundation of application of mineralized PA66/CaCl2 electrospun scaffolding material that structurally and chemically resembles native extracellular matrix for bone tissue engineering.

High strength polyvinyl alcohol/polyacrylic acid (PVA/PAA) hydrogel fabricated by Cold-Drawn method for cartilage tissue substitutes.

Poly (vinyl alcohol) (PVA) hydrogel has been considered as promising cartilage replacement materials due to its excellent characteristics such as high water content, low frictional behavior and excellent biocompatibility. However, lack of sufficient mechanical properties and cytocompatibility are two key obstacles for PVA hydrogel to be applied as cartilage substitutes. Herein, Polyacrylic acid (PAA) has been introduced into PVA hydrogel to balance these problems. Compared with pure PVA hydrogel, PVA/PAA hydrogel has the equal excellent biocompatibility, and its cell adhesion is significantly improved. In order to further improve the mechanical properties of hydrogels, Cold-Drawn treatment of hydrogels is performed in this paper. Compared to pure 12% PVA hydrogel, 40.8-fold, 50.8-fold, and 46.8-fold increase in tensile strength, tensile modulus, and toughness, respectively, which can be obtained from 12% PVA/PAA Cold-Drawn hydrogel. These biocompatible composite hydrogels have a great application potential as cartilage tissue substitutes.

Porous PVA/SA/HA hydrogels fabricated by dual-crosslinking method for bone tissue engineering.

In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method. The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail. The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure. The FTIR and XRD results indicate that PVA, SA and HA could be uniformly compounded. The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA. The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40. In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface. Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation. Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells. It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.