RESUMO
CASE: We performed arthroscopic superior capsule reconstruction (ASCR) for cuff tear arthropathy (CTA) with humeral head deformity. A 62-year-old man presented with severely limited shoulder motion and recalcitrant omalgia. He had a history of dental implant removal due to metal allergy, and his Constant score was 21 points. We diagnosed CTA with Hamada classification grade 5 and performed ASCR to avoid allergic reactions. Severe night pain improved within 1 week of ASCR, and his Constant score after 2 years was 74 points. CONCLUSION: ASCR may be an effective alternative treatment for patients with CTA with humeral head deformity.
Assuntos
Artroplastia/métodos , Lesões do Manguito Rotador/complicações , Artropatia de Ruptura do Manguito Rotador/cirurgia , Articulação do Ombro/cirurgia , Dor de Ombro/cirurgia , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Lesões do Manguito Rotador/diagnóstico por imagem , Articulação do Ombro/diagnóstico por imagem , Dor de Ombro/etiologiaRESUMO
Microfluidic systems have been developed for patterning single cells to study cell-cell interactions. However, patterning multiple types of cells to understand heterogeneous cell-cell interactions remains difficult. Here, it is aimed to develop a cell-trapping device to assemble multiple types of cells in the well-controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell-trapping area. The cell-trapping area is controlled by moving the parylene sheet on an SU-8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell-trapping. The assembly of cells on a parylene sheet with round and line-shaped apertures is demonstrated. The cell-cell contacts of the trapped cells are then investigated by direct cell-cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell-trapping area, three different types of cells in the well-controlled order are assembled. The correct cell order rate obtained using the device is 27.9%, which is higher than that obtained without the sliding parylene system (0.74%). Furthermore, the occurrence of cell-cell contact between the three cell types assembled is verified. This cell-patterning device will be a useful tool for investigating heterogeneous cell-cell interactions.